期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 155, 期 -, 页码 28-37出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2015.11.006
关键词
Tau-fluvalinate; Flumethrin; Calf thymus DNA; Pesticide; Groove binding; Spectroscopy
类别
资金
- National Natural Science Foundation of China [31460422, 21167013]
- Natural Science Foundation of Jiangxi Province [20143ACB20006, 20142BAB204001]
- Joint Specialized Research Fund for the Doctoral Program of Higher Education [20123601110005]
- Program of Jiangxi Provincial Department of Science and Technology [20141BBG70092]
- State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-ZZA-201302, SKLF-ZZB-201305]
Tau-fluvalinate (TFL) and flumethrin (FL), widely used in agriculture and a class of synthetic pyrethroid pesticides with a similar structure, may cause a potential security risk. Herein, the modes of binding in vitro of TFL and FL with calf thymus DNA (ctDNA) were characterized by fluorescence, UV-vis absorption, circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy with the aid of viscosity measurements, melting analyses and molecular docking studies. The fluorescence titration indicated that both TFL and FL bound to ctDNA forming complexes through hydrogen bonding and van der Waals forces. The binding constants of TFL and FL with ctDNA were in the range of 10(4) L mol(-1), and FL exhibited a higher binding propensity than TFL. The iodide quenching effect, single/double-stranded DNA effects, and ctDNA melting and viscosity measurements demonstrated that the binding of both TFL and FL to ctDNA was groove mode. The FT-IR analyses suggested the A-T region of the minor groove of ctDNA as the preferential binding for TFL and FL, which was confirmed by the displacement assays with Hoechst 33258 probe, and the molecular docking visualized the specific binding. The changes in CD spectra indicated that both FL and TFL induced the perturbation on the base stacking and helicity of B-DNA, but the disturbance caused by FL was more obvious. Gel electrophoresis analyses indicated that both TFL and FL did not cause significant DNA cleavage. This study provides novel insights into the binding properties of TFL/FL with ctDNA and its toxic mechanisms. (C) 2015 Elsevier B.V. All rights reserved.
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