期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 224, 期 -, 页码 290-297出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.10.044
关键词
Electrochemical genosensor; SH-ssDNA probe; RNA transcripts; Avian Influenza Virus H5N1; Screen printed gold electrodes; Miniaturized genosensor
资金
- Innovative Economy Program [POIG.01.01.02-00-007/08]
- Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland
The genosensors based on thiolated ssDNA probe deposited on the two types of gold electrodes: screen-printed (miniaturized) and disc electrodes destined for determination of specific sequences of DNA and RNA derived from Avian Influenza Virus H5N1 have been proposed. The working principle of genosensor is based on the ion-channel mechanism. The analytical signals generated upon hybridization processes were recorded using electrochemical technique - Osteryoung square wave voltammetry in the presence of a redox active marker [Fe(CN)(6)](3-/4-) in the sample solution. The miniaturized genosensor based on screen printed gold electrodes was able to detect the 20-mer complementary DNA oligonucleotide sequence as well as similar to 280-mer RNA sequences containing the complementary 20-mer sequence in various positions: at T-terminus, at 5'-terminus and in the middle of the RNA transcript at the 1 pM concentration. The measuring systems were selective. Non-complementary 20-mer oligonucleotide sequence as well as RNA transcript without complementary region generated weak response. The RNA transcripts were also tested with gold disc electrodes modified in the same manner. This device was able to detect similar to 280-mer RNA sequences, but at higher concentration of 10 pM. The good discrimination of the position of complementary part in the similar to 280-mer RNA sequences was observed with using both types of modified electrodes. (C) 2015 Published by Elsevier B.V.
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