期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 236, 期 -, 页码 815-824出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2015.12.099
关键词
Neuronal labeling; Neural microelectrode; Microiontophoresis; Neuroanatomical tracer injection; Biotinylated dextran amine
资金
- Hungarian Brain Research Program [KTIA NAP 13-2-2015-0004, KTIA 13 NAP A IV/1-4,6]
- National Research, Development & Innovation Office [TET_14_FR-1-2015-0030]
- Bolyai Janos Scholarship
- HAS
- Alexander von Humboldt Foundation
This paper presents the results of in vivo local release of a neuronal tracer, biotinylated dextran amine (BDA) in the rat somatosensory cortex using monolithically integrated microfluidic channel of a silicon neural microelectrode. The tracer injection is controlled by iontophoresis using Pt electrodes in the vicinity of the outlet of the microfluidic channel. Using 35 mu A, 57 s on/off cycle and 1520 min total injection time the localized injection resulted in clear anterograde and retrograde BDA labeling both within the cortex and in subcortical structures. Anterograde and retrograde labeling revealed the fine details of neuronal processes including dendritic spines and axon terminal-like endings. Injection sites appeared clear lacking any strong diffuse background labeling. Electrophysiological recording performed with the same microdevice immediately after the iontophoresis indicated normal cortical functioning. The results prove that the combination of in vivo multichannel neural recording and controlled tracer injection using a single implanted microdevice is feasible, and therefore it can be a powerful tool for studying the connectome of the brain. (C) 2016 Elsevier B.V. All rights reserved.
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