Article
Biochemistry & Molecular Biology
Qianshun Chen, Zuxiong Zhang, Li Xie, Chen Huang, Xing Lin, Wei Tang, Jiangxing Xu, Bin Qiu, Xunyu Xu
Summary: In this study, a one-step aptasensor for ultrasensitive detection of homocysteine (HCY) was developed using multifunctional carbon nanotubes, namely magnetic multi-walled carbon nanotubes (Fe3O4@MWCNTs) combined with an aptamer (Apt) for HCY (Fe3O4@MWCNTs-Apt). The Fe3O4@MWCNTs-Apt demonstrated multiple functions, including selective capture of target molecules, separation of captured molecules, and enhanced signal response. The aptasensor showed a good linear relationship between peak current of SWV and HCY concentration, with a satisfactory limit of detection. Successful detection of HCY in the plasma of lung cancer patients suggested the potential of this aptasensor for practical clinical applications.
BIOELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Yichun Xu, Xiaoyun Wang, Hongwei Chen, Li Chen, Wei Chen, Xinyang Yin, Ailin Liu, Xinhua Lin, Shaohuang Weng, Yanjie Zheng
Summary: The facile two-step SWV method combined with electrodeposition successfully prepared PtNPs/NPG modified electrode, which significantly improved the sensitivity and accuracy of miRNA-126 detection by using CHA. This testing method has potential value for early diagnosis of cancers.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Dong Liu, Shuyun Meng, Xiuli Shen, Yuye Li, Xiaohong Yan, Tianyan You
Summary: This work presents a dual-ratiometric aptasensor for streptomycin detection, utilizing the in-situ coupling of photoelectrochemical and electrochemical assays. The use of methylene blue as a bifunctional probe enables the simultaneous generation of photocurrent and redox current, resulting in enhanced analytical performances compared to single-signal or single-ratiometric assays. The MB-based dual-mode sensing offers a promising tool for studying interactions between PEC and EC behaviors.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Saeromi Chung, Jason K. Sicklick, Partha Ray, Drew A. Hall
Summary: An electrochemical sensor based on a conformation-changing aptamer has been developed to detect the cancer biomarker soluble KIT in human serum. The sensor shows high specificity and a wide dynamic range, successfully detecting KIT in different liquids.
Article
Chemistry, Analytical
Baozhong Zhang, Xinyue Ma, Lingling Xie, Xiquan Li, Lingyun Chen, Baoshan He
Summary: An ultrasensitive electrochemical aptasensor was developed in this study for the detection of tobramycin (TOB) residues. The sensor utilizes a signal amplification strategy and provides a broad linear range with a low detection limit. It exhibits favorable stability, specificity, and reproducibility, and has been successfully applied to practical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Lijun Luo, Xiaohong Liu, Xiaoya Bi, Libo Li, Tianyan You
Summary: In this study, the effects of methylene blue (MB) on the lumiphore and co-reactant of self-enhanced electrochemiluminescence (ECL) composites were investigated for the first time. The dual-quenching effects of MB on the ECL signal were ingeniously integrated and designed in a ratiometric aptasensor for zearalenone detection, significantly enhancing the sensitivity. This innovative ratiometric aptasensor achieved a wide linear range and a low detection limit, broadening the application in sensitive and precise analysis of mycotoxins.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Electrochemistry
Samet Sahin, Sevval Kaya, Zafer Ustundag, Mustafa Oguzhan Caglayan
Summary: An electrochemical signal switch aptasensor was successfully developed for sensitive detection of insulin. The fabricated aptasensor showed good detection performance in physiological conditions after optimizing the fabrication parameters. It exhibited a wide linear working range and low detection limit.
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Chengli Zhang, Xun You, Yuliang Li, Yuanyuan Zuo, Wei Wang, DanDan Li, ShengWei Huang, Hangzhi Hu, Feng Yuan, Fuqiang Shao, Minggang Yin
Summary: In this study, an aptamer-based amplification strategy using Ce-MOF and m-PdNFs-G4-MBs nanocomposite was developed for the sensitive detection of dopamine (DA). The results showed that the new detection platform achieved a minimum detection limit of 6 pmol/L for DA with good specificity. This platform has the potential to provide a convenient and efficient tool for the diagnosis of multiple neurological disorders in the future.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biophysics
Shubhangi Shukla, Pratik Joshi, Parand Riley, Roger J. Narayan
Summary: Square wave voltammetry is used to evaluate antigen-antibody coupling at the solid-liquid interface, and the modified electrodes with variable antibody surface coverage can be analyzed by changing current components. Detection of antigen coupling rate can be achieved with optimized variables.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Zhoubing Zhang, Xiaorun Jia, Xueqin Xu
Summary: A ratiometric electrochemical aptasensor based on gold nanoparticles (AuNPs) functionalization and hybridization chain reaction (HCR) has been designed for the detection of streptomycin. The aptasensor utilizes the binding of streptomycin aptamer and capture probe to detach ferrocene from the sensing interface, followed by the capture of methylene blue electrochemical probe through HCR. The use of ratiometric method improves the accuracy and sensitivity, enabling the detection of streptomycin in milk and honey samples.
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biology
George Oliveira, Jose Mauricio Schneedorf
Summary: The study conducted kinetic analysis of ricin-mediated adenine release using square wave voltammetry, revealing an unusual kinetic profile and a high catalytic efficiency.
Article
Chemistry, Analytical
Ma Lucia Abad-Gil, Ma Jesus Gismera, Teresa Sevilla, Jesus R. Procopio
Summary: In this study, the electrochemical behavior of MIT on a gold electrode was investigated and square wave voltammetric methods were developed for its determination at different concentration levels. MIT showed an irreversible and diffusion-controlled oxidation process, with pH-dependent potential and peak current values. By using square-wave voltammetry (SWV) and adsorptive stripping approach (SWAdSV), it is possible to determine MIT in water samples with high sensitivity and low detection limits. Both SWV and SWAdSV methods were successfully applied for the analysis of MIT in different water samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Jiansen Li, Fengzhen Yang, Xiaofeng Chen, Honggang Fang, Chuanyun Zha, Jingcheng Huang, Xia Sun, Mohamed Bedair Mohamed Ahmed, Yemin Guo, Yuan Liu
Summary: In this study, a dual-ratiometric electrochemical aptasensor was developed for simultaneous detection of malathion and profenofos. Metal ions, hairpin-tetrahedral DNA nano-structures, and nanocomposites were used to create the sensing framework and signal amplification strategy. The detection limits of the aptasensor for malathion and profenofos were 4.3 pg mL-1 and 13.3 pg mL-1, respectively.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Pachanuporn Sunon, Kamonwad Ngamchuea
Summary: A highly sensitive and rapid electrochemical sensor was developed using a molecularly imprinted polymer (MIP) for detecting melatonin. The MIP was synthesized through electropolymerization without the need for an initiating reagent. The sensor exhibited good selectivity for melatonin and showed excellent reproducibility and repeatability. It was successfully applied for detecting melatonin in urine and saliva samples with high recoveries.
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)