Article
Chemistry, Multidisciplinary
Federico Pini, Laura Frances-Soriano, Vittoria Andrigo, Marta Maria Natile, Niko Hildebrandt
Summary: The main challenge for UCNPs is to optimize their implementation in FRET biosensing and bioimaging for highly sensitive, wash-free, multiplexed, accurate, and precise quantitative analysis of biomolecules and biomolecular interactions. We have developed an analytical model that can determine the ideal UCNP-FRET system within a short period of time, resulting in significant improvements in sensitivity.
Article
Nanoscience & Nanotechnology
Tong Chen, Yunfei Shang, Yuyan Zhu, Shuwei Hao, Chunhui Yang
Summary: In this study, a novel upconversion nanostructure was explored for highly efficient FRET, achieving significant improvement in FRET efficiency by transferring excitation energy to an acceptor with the optimal doping ratio of lanthanide elements. The designed nanoprobe exhibited a lower detection limit and higher sensitivity, outperforming reported probes for detecting NO2-.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Mingmin Wu, Mengtian Zhang, Zhiwei Fan, Xinyue Qin, Xiaoxia Zhu, Haiwei Ji, Yuling Qin, Qi Wang, Li Wu
Summary: Sensitive detection of DNA methyltransferase activity using a new fluorescence transducer based on Forster resonance energy transfer (FRET) is presented, with high analytical performance and capability for enzyme inhibitor screening. The method offers a promising strategy for high-performance diagnosis, drug discovery, and treatment effectiveness evaluation in various disease-related methyltransferase activities.
Article
Biochemistry & Molecular Biology
Aleksey M. Krasnyi, Alsu A. Sadekova, Vlada V. Kometova, Valeriy V. Rodionov, Ekaterina L. Yarotskaya, Gennadiy T. Sukhikh
Summary: The DNA methylation profile of breast cancer differs from healthy tissues and can be used as a diagnostic and prognostic biomarker. The study aimed to compare gene methylation levels in small malignant breast cancer tumors, healthy tissue, and fibroadenoma, and to evaluate the effectiveness of the modified MS-HRM method for analysis. Differences in methylation levels were found for several genes, with a combination of four genes showing the highest diagnostic value for all breast cancer samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Dong-Min Kim, Jina Seo, Dong-Wook Kim, Woong Jeong, Sang-Hyun Hwang, Dong-Eun Kim
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Medical Laboratory Technology
Soo-Kyung Kim, John Jeongseok Yang, Sang-Hyun Hwang, Heungsup Sung, Sung Shin, Sun-Young Ko, Heung-Bum Oh
Summary: The study developed prediction programs for HLA-DQ antibodies in deceased donor kidney transplant patients, showing good accuracy. The pDQ DSA had high agreement and accuracy, and patients positive for DQ or pDQ DSA had significantly higher frequency of antibody-mediated rejection treatment than negative patients.
ANNALS OF LABORATORY MEDICINE
(2021)
Article
Biophysics
Andi Muhammad Ichzan, Sang-Hyun Hwang, Hyejin Cho, Chiew San Fang, Seonhwa Park, Gyeongho Kim, Jihyeon Kim, Ponnusamy Nandhakumar, Byeongjun Yu, Sangyong Jon, Kwang-sun Kim, Haesik Yang
Summary: The study successfully overcame the challenges of low detection limits in solid-phase RPA by combining it with electrochemical detection and using a new primer concentration combination. Experimental results on hepatitis B virus demonstrated that this method achieved better amplification efficiency for HBV genomic DNA compared to other methods.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Virology
Yasmin Hisham, Yaqoub Ashhab, Sang-Hyun Hwang, Dong-Eun Kim
Summary: An in silico workflow pipeline was established to identify B-cell- and T-cell-stimulating antigens of SARS-CoV-2 viral proteins. Thirteen potential epitopes were selected as subunit vaccine candidates through a reverse vaccinology approach, despite mutational amino acid substitutions.
Article
Cell Biology
John Jeongseok Yang, Su Jin Lee, Yu Jin Lee, Dae-Hyun Ko, Young-Uk Cho, Seongsoo Jang, Chan-Jeoung Park, Heung-Bum Oh, Sang-Hyun Hwang
BIOPRESERVATION AND BIOBANKING
(2022)
Article
Chemistry, Analytical
Islam Seder, Dong-Min Kim, Sang-Hyun Hwang, Heungsup Sung, Dong-Eun Kim, Sung-Jin Kim
Summary: The article discusses a microfluidic platform for the sample-to-answer detection of viral RNA from clinical swab samples in a fully automated and highly sensitive manner. This platform allows for accurate diagnosis of infectious diseases in point-of-care settings.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Letter
Medical Laboratory Technology
Bosung Park, Jin Seok Kim, Hee-Jeong Youk, Yousun Chung, Hyungsuk Kim, Sang-Hyun Hwang, Heung-Bum Oh, Dae-Hyun Ko
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Chemistry, Analytical
Soo-Kyung Kim, Yoon-Hee Oh, Dae-Hyun Ko, Heungsup Sung, Heung-Bum Oh, Sang-Hyun Hwang
Summary: Rapid, simple, and inexpensive diagnostic point-of-care tests are crucial for controlling infectious diseases in resource-limited settings. In this study, a new detection system based on nanoparticle-DNA aggregation was developed for visual detection of hepatitis C virus. The results showed that the system had a high limit of detection and analytical specificity, and it was comparable to the standard real-time PCR assay.
Article
Chemistry, Analytical
Jamin Ku, Khushbu Chauhan, Sang-Hyun Hwang, Yong-Joo Jeong, Dong-Eun Kim
Summary: Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that allows the simple, quick, and low-cost detection of various viral genes. However, LAMP assays are susceptible to generating non-specific amplicons. In this study, it was found that poly(ethylene glycol)-engrafted nanosized graphene oxide (PEG-nGO) can significantly enhance the specificity of LAMP by adsorbing surplus ssDNA primers, reducing non-specific annealing. The inclusion of PEG-nGO greatly improves the specificity and sensitivity of the LAMP assay, facilitating the detection of HCV-positive clinical samples with superior precision compared to RT-qPCR.
Letter
Medical Laboratory Technology
Jiyeon Kim, Miyoung Kim, Young-Uk Cho, Sang-Hyun Hwang, Seongsoo Jang, Eul-Ju Seo, Dok Hyun Yoon, Heounjeong Go, Chan-Jeoung Park
ANNALS OF LABORATORY MEDICINE
(2023)
Letter
Medical Laboratory Technology
Jeayeon Ryu, Jihyun Choi, Miyoung Kim, Young -Uk Cho, Sang-Hyun Hwang, Seongsoo Jang, Chan-Jeoung Park
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Hyunji Kim, Sung Shin, Sang-Hyun Hwang, Miyoung Kim, Young-uk Cho, Seongsoo Jang
Summary: The adoption of high-sensitivity flow cytometry (HSFC) has been slow in routine laboratory settings due to concerns about result reliability and reproducibility. This study aimed to validate an HSFC protocol for detecting Tfh cells in a real-world laboratory environment. The Tfh cell panel was analytically validated using rigorous testing, and concerns about result reliability and reproducibility in real-world laboratories were addressed through systematic validation. Strategic validation of flow cytometry panels can facilitate the adoption of HSFC in clinical laboratories, even with limited resources.
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Sang Ki Kim, Jong Uk Lee, Myeong Jin Jeon, Soo-Kyung Kim, Sang-Hyun Hwang, Min Eui Hong, Sang Jun Sim
Summary: A colorimetric-fluorescent dual-mode lateral flow immunoassay sensor incorporating metal-enhanced fluorescence (MEF) was developed for the rapid and sensitive serological detection of SARS-CoV-2 IgG.
Article
Biochemistry & Molecular Biology
Jung Min Kim, Eunbi Yi, Hyungwoo Cho, Woo Seon Choi, Dae-Hyun Ko, Dok Hyun Yoon, Sang-Hyun Hwang, Hun Sik Kim
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Gastroenterology & Hepatology
Seri Hong, Hye Young Shin, Bomyee Lee, Na Rae Hwang, Sang-Hyun Hwang, Jae Kwan Jun