Article
Medicine, General & Internal
Brooke L. Deatherage Kaiser, Dawn N. Birdsell, Janine R. Hutchison, Johanna Thelaus, Sarah C. Jenson, Voahangy Andrianaivoarimanana, Mona Bystroem, Kerstin Myrtennaes, Ryelan F. McDonough, Roxanne D. Nottingham, Jason W. Sahl, Herbert P. Schweizer, Minoarisoa Rajerison, Mats Forsman, David S. Wunschel, David M. Wagner
Summary: Antimicrobial resistance is a growing concern, and we studied the proteins associated with Yersinia pestis and Francisella tularensis strains to identify potential protein signatures for antimicrobial resistance (AMR). We found that protein expression was significantly influenced by AMR, even in the absence of antibiotics in growth media. Additionally, the abundance of specific proteins involved in metabolic pathways and biological functions was altered in AMR strains, regardless of species or resistance mechanism. These features could be used to develop novel approaches for identifying AMR phenotypes.
FRONTIERS IN MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Hyun-Joong Kim, Hee-Won Yoon, Min-A Lee, Young-Hoon Kim, Chang Joo Lee
Summary: Ensuring the supply of microbiological risk-free water is crucial for food safety and public hygiene. This study evaluated the disinfection efficiencies of UV-C irradiation on Shigella flexneri and Listeria monocytogenes in drinking water using a water purifier. The findings revealed that UV-C irradiation effectively disinfects microbial contaminants in drinking water and provided insights into the bacterial features and responses after UV-C irradiation.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Lalitha Tadala, Dorothee Langenbach, Mirjam Dannborg, Ramon Cervantes-Rivera, Atin Sharma, Kevin Vieth, Lisa M. Rieckmann, Alkwin Wanders, David A. Cisneros, Andrea Puhar
Summary: This study demonstrates that the mechanosensitive plasma membrane channel PIEZO1 acts as a sensor for bacterial entry and initiates immune signaling through ATP secretion and gene expression. It suggests that the detection of infection by PIEZO1 is driven by physical signals instead of chemical ligands, independent of detection of microbial molecules.
Article
Multidisciplinary Sciences
Gina M. Borgo, Thomas P. Burke, Cuong J. Tran, Nicholas T. N. Lo, Patrik Engstrom, Matthew D. Welch
Summary: This study identified a phospholipase enzyme in Rickettsia bacteria that plays an important role in the invasion and spread of infection by helping the bacteria escape from host cell vacuoles, preventing targeting by autophagy, and promoting bacterial motility and spread to other cells.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Josephine Moran, Liam Feltham, James Bagnall, Marie Goldrick, Elizabeth Lord, Catherine Nettleton, David G. Spiller, Ian Roberts, Pawel Paszek
Summary: The paper investigates the real-time interaction between the food-borne pathogen L. monocytogenes and host macrophages, revealing heterogeneous outcomes of infection and the impact of bacterial quantity on macrophage phagocytosis ability. It also highlights the role of population-level PrfA-mediated activity in regulating host-pathogen interactions.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Wieslaw Swietnicki
Summary: The review discusses strategies for designing vaccines against selected pathogens using bacterial secretory systems and connects them to the biology of the system. Examples are given for Y. pestis, S. enterica, B. anthracis, S. flexneri, and other human pathogens, and their effectiveness and long-term protection are discussed.
Article
Medicine, Research & Experimental
A. Eskova, A. A. Yakovlev, A. Kim
Summary: The study found that marine saprotrophic bacteria Flavobacterium sp. and Micrococcus luteus can form mixed-species biofilms with pathogenic bacteria such as Yersinia pseudotuberculosis and Listeria monocytogenes, promoting the growth of the pathogens and potentially contributing to their preservation in the marine environment.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Jong-Seong Lim, Jae-Won Ha
Summary: This study evaluates the effect of different growth temperatures on the resistance of Listeria monocytogenes and Yersinia enterocolitica to low-energy X-ray irradiation and elucidates the mechanisms of resistance variability. The results indicate that the increased resistance to X-ray irradiation at low temperatures in the two pathogens is induced by different mechanisms.
Article
Immunology
Martha A. L. Boening, Gerald P. Parzmair, Andreas Jeron, Henning P. Duesedau, Olivia Kershaw, Baolin Xu, Borna Relja, Dirk Schlueter, Ildiko Rita Dunay, Annegret Reinhold, Burkhart Schraven, Dunja Bruder
Summary: Mice lacking the adhesion and degranulation-promoting adaptor protein (ADAP) are highly susceptible to infection by the intracellular pathogen Listeria monocytogenes (Lm), showing enhanced immunopathology and increased infiltration of immune cells. Despite high phagocyte numbers in infected tissues, ADAP-deficient mice struggle to control pathogen growth efficiently, indicating a functional impairment in infection-primed phagocytes. Analysis revealed molecular alterations in pro-inflammatory mediators following activation in ADAP-deficient host, leading to enhanced immunopathology and reduced pathogen elimination capacity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Shahar Rotem, Ohad Shifman, Moshe Aftalion, David Gur, Tamar Aminov, Ronit Aloni-Grinstein
Summary: The rapid antibiotic susceptibility test method MAPt, which can be performed directly on whole blood samples, offers advantages by omitting time-consuming steps, determining minimal inhibitory concentration quickly, and improving rapid diagnosis and treatment of pathogens.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
I. Ziegler, P. Vollmar, M. Knuepfer, P. Braun, K. Stoecker
Summary: The study aimed to determine and compare the limits of detection (LOD) of several commercially available LFAs using viable risk group-3 strains. The results showed significant variations in LODs between different tests and organisms, highlighting the importance of using accurately quantified viable strains for determining LODs.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Review
Microbiology
Vishnu Raman, Chinmay P. Deshpande, Shradha Khanduja, Lars M. Howell, Nele Van Dessel, Neil S. Forbes
Summary: Bacterial delivery platforms can improve the safety and prolong the treatment time of cancer therapies. By genetically modifying bacteria, they can express a range of therapies that match multiple cancer indications. Research on gut microbe pathogenesis has led to the development of safe bacterial therapies.
CELL HOST & MICROBE
(2023)
Article
Multidisciplinary Sciences
Damian Lobato-Marquez, Jingwei Xu, Gizem Oezbaykal Gueler, Adaobi Ojiakor, Martin Pilhofer, Serge Mostowy
Summary: Septins are cytoskeletal proteins that form cage-like structures to restrict the movement of bacteria within cells. Research shows that septin complexes can recognize bacterial cells and assemble as filaments on their surface.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
E. Amini, F. Salimi, S. Imanparast, F. N. Mansour
Summary: This study aimed to find lactic acid bacteria that can produce antibacterial exopolysaccharide (EPS) against foodborne pathogens. The EPS produced by the AS20(1) strain showed inhibitory effects on the growth of Listeria monocytogenes, Yersinia enterocolitica, and Bacillus cereus. The AS20(1) strain was identified as Lacticaseibacillus paracasei and showed probiotic properties. The antibacterial EPS had various functional groups, an amorphous structure, and a smooth surface, making it suitable for food packaging.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Alberto Schiraldi, Roberto Foschino
Summary: The two-parameter Schiraldi's model fits growth curves of psychrotrophic pathogens more reliably than the Baranyi and Roberts' model. The increase of temperature has a milder effect on the growth rate than expected. Parameter beta defines the number of cell duplications and appears to be independent of temperature.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Immunology
Xin Tan, Mathieu Coureuil, Elodie Ramond, Daniel Euphrasie, Marion Dupuis, Fabiola Tros, Julie Meyer, Ivan Nemazanyy, Cerina Chhuon, Ida Chiara Guerrera, Agnes Ferroni, Isabelle Sermet-Gaudelus, Xavier Nassif, Alain Charbit, Anne Jamet
CLINICAL INFECTIOUS DISEASES
(2019)
Article
Infectious Diseases
Charles Vidal, Florence Moulin, Xavier Nassif, Louise Galmiche, Delphine Borgel, Alain Charbit, Capucine Picard, Jean-Paul Mira, Olivier Lortholary, Anne Jamet, Julie Toubiana
BMC INFECTIOUS DISEASES
(2019)
Article
Immunology
Xin Tan, Elodie Ramond, Anne Jamet, Jean-Philippe Barnier, Baptiste Decaux-Tramoni, Marion Dupuis, Daniel Euphrasie, Fabiola Tros, Ivan Nemazanyy, Jason Ziveri, Xavier Nassif, Alain Charbit, Mathieu Coureuil
JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Biochemical Research Methods
Jason Ziveri, Cerina Chhuon, Anne Jamet, Heloise Rytter, Guenole Prigent, Fabiola Tros, Monique Barel, Mathieu Coureuil, Claire Lays, Thomas Henry, Nicholas H. Keep, Ida Chiara Guerrera, Alain Charbit
MOLECULAR & CELLULAR PROTEOMICS
(2019)
Editorial Material
Biochemistry & Molecular Biology
Xin Tan, Mathieu Coureuil, Alain Charbit, Anne Jamet
TRENDS IN MICROBIOLOGY
(2020)
Article
Microbiology
Anne Jamet, Julien Guglielmini, Bryan Brancotte, Mathieu Coureuil, Daniel Euphrasie, Julie Meyer, Johanna Roux, Jean-Philippe Barnier, Emmanuelle Bille, Agnes Ferroni, Jean-Francois Magny, Christine Bole-Feysot, Alain Charbit, Xavier Nassif, Sylvain Brisse
Summary: The study developed a core genome multilocus sequence typing (cgMLST) scheme for Staphylococcus epidermidis, which proved effective in identifying and tracking the long-lasting endemic persistence of S. epidermidis clones within and across hospital wards. The publicly available tool may enable international harmonization of epidemiological surveillance of multidrug-resistant S. epidermidis clones, with the identification of gene distribution differences between infection and commensal isolates.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Review
Microbiology
Heloise Rytter, Anne Jamet, Mathieu Coureuil, Alain Charbit, Elodie Ramond
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Elodie Ramond, Anne Jamet, Xiongqi Ding, Daniel Euphrasie, Clemence Bouvier, Louison Lallemant, Xiangyan He, Laurence Arbibe, Mathieu Coureuil, Alain Charbit
Summary: The study established a Drosophila melanogaster larvae model for oral S. aureus infection, revealing that S. aureus infection stimulates host immunity and the catalase of S. aureus plays a key role in this complex environment. It is proposed that fly larvae can provide a general model for studying the colonization capabilities of human pathogens.
Article
Microbiology
Heloise Rytter, Anne Jamet, Jason Ziveri, Elodie Ramond, Mathieu Coureuil, Pauline Lagouge-Roussey, Daniel Euphrasie, Fabiola Tros, Nicolas Goudin, Cerina Chhuon, Ivan Nemazanyy, Fabricio Edgar de Moraes, Carlos Labate, Ida Chiara Guerrera, Alain Charbit
Summary: Metabolic pathways are crucial to the virulence of pathogenic bacteria and potential targets for antibacterial interventions. The pentose phosphate pathway (PPP) plays a key role in the intracellular survival of Francisella novicida, connecting with multiple other metabolic networks. Proteomics and metabolomics analyses show that PPP is a major hub linking glycolysis, the tricarboxylic acid cycle, fatty acid degradation, and sulfur metabolism, highlighting its importance in Francisella's pathogenesis and growth.
Article
Biochemical Research Methods
Vincent Jung, Kevin Roger, Cerina Chhuon, Louise Pannetier, Joanna Lipecka, Josue Sulub Gomez, Pascal Chappert, Alain Charbit, Ida Chiara Guerrera
Summary: This work combines biolayer interferometry (BLI) technique with mass spectrometry (MS) to demonstrate the feasibility of BLI-MS in complex biological mixtures.
Article
Immunology
Elodie Ramond, Agathe Lepissier, Xiongqi Ding, Clemence Bouvier, Xin Tan, Daniel Euphrasie, Pierre Monbernard, Marion Dupuis, Bruno Saubamea, Ivan Nemazanyy, Xavier Nassif, Agnes Ferroni, Isabelle Sermet-Gaudelus, Alain Charbit, Mathieu Coureuil, Anne Jamet
Summary: This study investigates the role of persistent Staphylococcus aureus clones in cystic fibrosis (CF) disease. The results suggest that the lung-adapted variants of S. aureus may promote inflammation and contribute to the progression of chronic lung disease.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Xiongqi Ding, Xiali Fu, Daniel Euphrasie, Agnes Ferroni, Isabelle Sermet-Gaudelus, Alain Charbit, Mathieu Coureuil, Anne Jamet
Summary: This study investigated the virulence and antimicrobial resistance-associated genes and within-host evolution polymorphisms in 14 persistent clones of Staphylococcus aureus from chronically infected children with cystic fibrosis. The study identified convergent mutations in genes involved in intracellular invasion and persistence, which play an important role in the long-term persistence ability of S. aureus.
MICROBES AND INFECTION
(2023)
Article
Chemistry, Multidisciplinary
Mar Eroles, Javier Lopez-Alonso, Alexandre Ortega, Thomas Boudier, Khaldoun Gharzeddine, Frank Lafont, Clemens M. Franz, Arnaud Millet, Claire Valotteau, Felix Rico
Summary: Monocytes activated by pro-inflammatory signals adhere to the vascular endothelium and migrate from the bloodstream to the tissue ultimately differentiating into macrophages. Cell mechanics and adhesion play a crucial role in macrophage functions during this inflammatory process. However, how monocytes change their adhesion and mechanical properties upon differentiation into macrophages is still not well understood.
Article
Gastroenterology & Hepatology
S. Salome-Desnoulez, S. Poiret, B. Foligne, G. Muharram, V Peucelle, F. Lafont, C. Daniel
Summary: The study used fluorescently labeled L. plantarum strains to monitor the persistence of these bacteria in inflamed guts using both noninvasive whole-body imaging and ex vivo fluorescence confocal microscopy. The results showed that orally administered anti-inflammatory strains persisted longer and at higher counts in the inflamed colon, and in more severely inflamed areas they were in direct contact with damaged epithelial cells.
Article
Chemistry, Multidisciplinary
Sebastien Janel, Michka Popoff, Nicolas Barois, Elisabeth Werkmeister, Severine Divoux, Franck Perez, Frank Lafont
Review
Cell Biology
Alyssa Kearly, Andrew D. L. Nelson, Aleksandra Skirycz, Monika Chodasiewicz
Summary: Stress Granules (SGs) and Processing-bodies (P-bodies) are important biomolecular condensates that play crucial roles in maintaining mRNA balance and regulating stress responses. They are composed of proteins and RNAs involved in translation, protein folding, and energy metabolism.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
P. Lemonnier, T. Lawson
Summary: Stomatal conductance plays a crucial role in determining CO2 uptake and water loss in plants, affecting overall water status and productivity. However, the signals coordinating mesophyll demands for CO2, the role of chloroplasts in stomatal function, and other GC metabolic processes in stomatal function remain poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Matteo Gionfriddo, Timothy Rhodes, Spencer M. Whitney
Summary: Rubisco is a key enzyme that facilitates the entry of CO2 into the biosphere, but its catalytic properties are slow and error-prone. More effective Rubisco variants have been discovered in certain algae, offering the potential to significantly improve crop productivity. However, incompatibilities in protein folding have hindered the transplantation of these variants into plants. Directed evolution is now being explored to enhance Rubisco catalysis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Vittoria Clapero, Stephanie Arrivault, Mark Stitt
Summary: The Calvin-Benson cycle has undergone massive selection and co-evolution with carbon-concentrating mechanisms due to changing environmental factors. Metabolite profiling reveals species-specific variations in the operation of the cycle, indicating the influence of different modes of photosynthesis. Connectivity analysis identifies constraints and driving factors for cross-species diversity in the cycle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Sukhbir Kaur, David D. Roberts
Summary: Thrombospondin-1 modulates cell behavior by interacting with components of the extracellular matrix and cell surface receptors. Its release and expression are influenced by injuries and various diseases, while its sustained presence in the extracellular space is regulated by receptor-mediated clearance. Thrombospondin-1 plays important roles in immune responses.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Richard P. Tucker, Josephine C. Adams
Summary: Thrombospondins (TSPs) play diverse roles in animals and have been found to belong to a superfamily that includes different subgroups such as mega-TSPs, sushi-TSPs, and poriferan-TSPs. Invertebrates encode a greater diversity of TSP superfamily members than vertebrates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
James Petrik, Sylvia Lauks, Bianca Garlisi, Jack Lawler
Summary: Many cancers start with a small nest of transformed cells that can remain dormant. Thrombospondin-1 (TSP-1) initially promotes dormancy by suppressing angiogenesis, but over time, factors promoting angiogenesis become dominant and recruit various cells to form a complex tumor microenvironment. TSPs play a role in the proliferation, migration, and invasion of cells in the tumor microenvironment, as well as influencing the immune characteristics and phenotype of tumor-associated macrophages.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hana Fakim, Christine Vande Velde
Summary: There has been increasing attention to the role of phase-separated biomolecular condensates, specifically stress granules, in neurodegenerative diseases like ALS. ALS-associated mutations in genes involved in stress granule assembly have been found, and stress granule proteins have been detected in pathological inclusions in ALS patient neurons. However, protein components of stress granules are also present in other physiological biomolecular condensates, which have not been adequately discussed in relation to ALS. This review explores the functions of TDP-43 and FUS in physiological condensates occurring in the nucleus and neurites beyond stress granules, and discusses the impact of ALS-linked mutations on their ability to phase separate and perform their functions in stress-independent biomolecular condensates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Alexander Lin, Yogambha Ramaswamy, Ashish Misra
Summary: Smooth muscle cells, endothelial cells, and macrophages in blood vessels display remarkable heterogeneity, and their developmental origins may influence their plasticity. Unbiased single cell whole transcriptome analysis techniques are revolutionizing the understanding of cellular diversity and plasticity, providing insights for therapeutic research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Elton P. Hudson
Summary: The Calvin Benson cycle plays a crucial role in the ecological and biotechnological aspects of bacteria. Recent studies have shed light on the regulation of this cycle in bacteria, with post-transcriptional and post-translational regulation being important in phototrophic bacteria, and transcriptional regulation being prominent in chemolithoautotrophic bacteria. Understanding the regulation of the Calvin Benson cycle has implications for enhancing CO2 fixation and improving the synthesis of desired products. Non-canonical cycles may offer potential benefits for industrial applications.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Xin-Guang Zhu, Haim Treves, Honglong Zhao
Summary: This paper discusses the major regulatory mechanisms over the Calvin Benson Cycle (CBC) that maintain homeostasis of metabolite levels. These mechanisms include redox regulation of enzymes, metabolite regulations (especially allosteric regulations), and balanced activities of enzymes. These regulatory mechanisms are crucial for maintaining high flux and photosynthetic efficiency in CBC.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hunter C. Herriage, Yi-Ting Huang, Brian R. Calvi
Summary: Apoptosis prevents the inappropriate acquisition of extra copies of the genome, known as polyploidy, but the polyploid state can suppress apoptosis. The mechanisms linking apoptosis and polyploid cell cycles are still largely unknown, and studying the regulation of apoptosis in development and cancer could lead to more effective therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Daniel Campbell, Steven Zuryn
Summary: Mitochondrial dynamics play a crucial role in regulating cellular and organismal homeostasis, impacting various aspects of an organism's healthspan. By studying the nematode Caenorhabditis elegans, a comprehensive understanding of the impact of mitochondrial dynamics on homeostasis over a lifetime can be obtained.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Diba Borgmann, Henning Fenselau
Summary: Maintaining blood glucose at an appropriate physiological level requires coordination of multiple organs and tissues, with the vagus nerve playing a key role in central control. Recent studies have revealed the cellular identity, neuroanatomical organization, and functional contributions of vagal neurons in the regulation of systemic glucose metabolism. These findings provide new insights into the precise roles of vagal neurons in coordinating glucose levels and offer potential avenues for treating glucose metabolism disorders.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Tatiana C. Coverdell, Stephen B. G. Abbott, John N. Campbell
Summary: In this article, we review how genetic technology and single-cell genomics are revealing the organizational principles of the efferent vagus in unprecedented detail.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
