Article
Cell Biology
Shreya Rajachandran, Xin Zhang, Qiqi Cao, Andre L. Caldeira-Brant, Xiangfan Zhang, Youngmin Song, Melanie Evans, Orhan Bukulmez, Edward J. Grow, Makoto Nagano, Kyle E. Orwig, Haiqi Chen
Summary: In this study, the molecular, cellular, and spatial composition of SSC niches was systematically dissected using spatial transcriptomics, computational analyses, and functional assays. The results showed that pleiotrophin regulates mouse SSC functions through syndecan receptors, and ephrin-A1 may be a niche factor that influences human SSC functions. Moreover, the spatial re-distribution of inflammation-related LR interactions was found to underlie diabetes-induced testicular injury. This study provides a systems approach to understanding the complex organization of the stem cell microenvironment in health and disease.
Article
Environmental Sciences
XueXia Liu, ZhiXin Wang, Fujun Liu
Summary: Mice treated with BPA exhibited decreased sperm quality, lower serum testosterone levels, and a sub-fertile phenotype, mainly due to impaired germ cell proliferation. BPA had a concentration-dependent inhibition effect on germ cell proliferation, leading to reduced male fertility.
Article
Cell Biology
Yoshifumi Mori, Seiji Takashima, Mito Kanatsu-Shinohara, Zheng Yi, Takashi Shinohara
Summary: The study found that Cdc42 is essential for the development of the germline niche, as its deficiency results in a significant reduction in the number of spermatogonial stem cells in the testes, affecting GDNF expression and Sertoli cell development.
Article
Agriculture, Dairy & Animal Science
Yi Zheng, Qiang Gao, Tianjiao Li, Ruifang Liu, Zechao Cheng, Ming Guo, Jinhong Xiao, De Wu, Wenxian Zeng
Summary: The study systematically investigated the development patterns of Sertoli cells and spermatogonia in pigs from neonate to sexual maturity, shedding light on previously unknown details. The findings not only expand knowledge about spermatogenesis and testicular development in pigs, but also provide a theoretical basis for porcine breeding and rearing.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Hue M. La, Jinyue Liao, Julien M. D. Legrand, Fernando J. Rossello, Ai-Leen Chan, Vijesh Vaghjiani, Jason E. Cain, Antonella Papa, Tin Lap Lee, Robin M. Hobbs
Summary: This study uncovers the roles of growth factor signalling and mTORC1 in the regeneration of spermatogonial stem cells (SSCs). Single-cell analysis reveals the unique molecular features of regenerative SSCs and the changes in the composition of undifferentiated spermatogonia during germline recovery. The data suggest that transient mTORC1 activation is critical for the regenerative response, while sustained mTORC1 signalling is detrimental for SSC maintenance. Inhibition of growth factor signalling disrupts the regenerative state and limits germline recovery.
NATURE COMMUNICATIONS
(2022)
Article
Obstetrics & Gynecology
Amir Hossein Hasani Fard, Mahmoud Valizadeh, Zohreh Mazaheri, Seyed Jalil Hosseini
Summary: This study found that miR-106b-5p enhances the proliferative potential of SSCs and has significant value in the treatment of male infertility.
REPRODUCTIVE SCIENCES
(2022)
Article
Developmental Biology
Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick, P. Hande Ozdinler, Douglas A. Gray, Kelsey Fisher-Wellman, Brian P. Hermann, Christopher B. Geyer
Summary: We have developed a facile in vitro differentiation and meiotic initiation system that can be manipulated using chemical agents that cannot be administered to live animals. Additionally, we introduced a transgenic mouse model enabling the isolation of specific developmental stages of spermatogonia and meiotic spermatocytes.
Article
Biochemistry & Molecular Biology
Zhiming Shen, Min Chen, Yang Gao, Fangfang Dong, Changhuo Cen, Haowei Wu, Nan Wang, Xiuhong Cui, Chunsheng Han, Fei Gao
Summary: This study found that inactivation of Foxo1 after birth leads to germ cell loss and male infertility. Foxo1 is involved in the development of spermatogonia after birth, and its function is probably not regulated by the PI3K/PTEN signaling pathway.
Article
Developmental Biology
Na Liu, Fatimunnisa Qadri, Hauke Busch, Stefanie Huegel, Gabin Sihn, Ilya Chuykin, Enno Hartmann, Michael Bader, Franziska Rother
Summary: The protein Kpna6 plays a crucial role in spermatogenesis and male fertility, with its deficiency leading to infertility and various effects on sperm and Sertoli cells. Its absence results in impaired Sertoli cell function, incomplete sperm maturation, and altered expression of genes involved in sperm production.
Article
Agriculture, Dairy & Animal Science
Ke Hu, Yuanyuan Gao, Yifan Xu, Chaofan He, Kaixian Wang, Leina Li, Yaping Liao, Xiaohua Liu, Meng Liang
Summary: This study reveals the elevated expression of lncRNA-Gm2044 in nonobstructive azoospermia and its partial impairment on spermatogenesis in a transgenic mouse model. Transcriptome sequencing analysis uncovers the potential signaling pathways involved in the repression of spermatogenesis. The findings suggest that lncRNA-Gm2044 is a novel molecular target for the diagnosis and treatment of male infertility.
Review
Cell Biology
Peiting Mo, Ziran Zhao, Xinpeng Ke, Yong Fan, Chaohui Li
Summary: An increasing number of men requiring long-term drug therapy has raised concerns about its impact on male fertility. However, there is a lack of evaluation in clinical practice. This article reviews the effects of small molecule medications on male fertility and explores the potential of stem cell therapy and exosomes treatment in addressing male infertility, aiming to provide recommendations for evaluating the effects of drugs on male fertility and strategies for diagnosis and treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Wasim Shah, Ranjha Khan, Basit Shah, Asad Khan, Sobia Dil, Wei Liu, Jie Wen, Xiaohua Jiang
Summary: The process of spermatogenesis relies on hormones and growth factors acting through endocrine and paracrine pathways. Sertoli cells are major regulators, while sex hormones play a significant role in determining their proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Zongzhuang Wen, Haixia Zhu, Jing Wang, Bin Wu, Aizhen Zhang, Hui Zhao, Chenyang Song, Shuangyuan Liu, Yin Cheng, Hongxiang Wang, Jianyuan Li, Daqing Sun, Xiaolong Fu, Jiangang Gao, Min Liu
Summary: This study generated a germ cell-specific deletion mutant of the Hspa5 gene (Hspa5-cKO) using CRISPR/Cas9 technology and the Cre/Loxp system. Hspa5 knockout resulted in severe germ cell loss and vacuolar degeneration of seminiferous tubules, leading to complete arrest of spermatogenesis, testicular atrophy, and male infertility in adult mice. Single-cell RNA sequencing analysis further confirmed that HSPA5 is crucial for germ cell development.
Article
Oncology
Yibo Wu, Ranran Zhang, Cong Shen, Jinfu Xu, Tiantian Wu, Xiaoyan Huang, Mingxi Liu, Hong Li, Dewu Xu, Bo Zheng
Summary: This study suggests that the Asb15 gene is not essential for spermatogenesis or fertility in mice. These findings can prevent redundant research by other scientists and provide new information for further studies on the genetics of fertility in humans.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2022)
Article
Reproductive Biology
Xianrong Xiong, Xiangyue Huang, Yanjin Zhu, Zhuo Hai, Xixi Fei, Bangting Pan, Qinhui Yang, Yan Xiong, Wei Fu, Daoliang Lan, Xiaojian Zhang, Jian Li
Summary: By establishing a testis-specific knockout model of Kdm2a, it was found that the loss of Kdm2a did not completely compromise sperm production, possibly due to compensatory effects from its homologous gene Kdm2b.
Review
Cell Biology
Alyssa Kearly, Andrew D. L. Nelson, Aleksandra Skirycz, Monika Chodasiewicz
Summary: Stress Granules (SGs) and Processing-bodies (P-bodies) are important biomolecular condensates that play crucial roles in maintaining mRNA balance and regulating stress responses. They are composed of proteins and RNAs involved in translation, protein folding, and energy metabolism.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
P. Lemonnier, T. Lawson
Summary: Stomatal conductance plays a crucial role in determining CO2 uptake and water loss in plants, affecting overall water status and productivity. However, the signals coordinating mesophyll demands for CO2, the role of chloroplasts in stomatal function, and other GC metabolic processes in stomatal function remain poorly understood.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Matteo Gionfriddo, Timothy Rhodes, Spencer M. Whitney
Summary: Rubisco is a key enzyme that facilitates the entry of CO2 into the biosphere, but its catalytic properties are slow and error-prone. More effective Rubisco variants have been discovered in certain algae, offering the potential to significantly improve crop productivity. However, incompatibilities in protein folding have hindered the transplantation of these variants into plants. Directed evolution is now being explored to enhance Rubisco catalysis.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Vittoria Clapero, Stephanie Arrivault, Mark Stitt
Summary: The Calvin-Benson cycle has undergone massive selection and co-evolution with carbon-concentrating mechanisms due to changing environmental factors. Metabolite profiling reveals species-specific variations in the operation of the cycle, indicating the influence of different modes of photosynthesis. Connectivity analysis identifies constraints and driving factors for cross-species diversity in the cycle.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Sukhbir Kaur, David D. Roberts
Summary: Thrombospondin-1 modulates cell behavior by interacting with components of the extracellular matrix and cell surface receptors. Its release and expression are influenced by injuries and various diseases, while its sustained presence in the extracellular space is regulated by receptor-mediated clearance. Thrombospondin-1 plays important roles in immune responses.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Richard P. Tucker, Josephine C. Adams
Summary: Thrombospondins (TSPs) play diverse roles in animals and have been found to belong to a superfamily that includes different subgroups such as mega-TSPs, sushi-TSPs, and poriferan-TSPs. Invertebrates encode a greater diversity of TSP superfamily members than vertebrates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
James Petrik, Sylvia Lauks, Bianca Garlisi, Jack Lawler
Summary: Many cancers start with a small nest of transformed cells that can remain dormant. Thrombospondin-1 (TSP-1) initially promotes dormancy by suppressing angiogenesis, but over time, factors promoting angiogenesis become dominant and recruit various cells to form a complex tumor microenvironment. TSPs play a role in the proliferation, migration, and invasion of cells in the tumor microenvironment, as well as influencing the immune characteristics and phenotype of tumor-associated macrophages.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hana Fakim, Christine Vande Velde
Summary: There has been increasing attention to the role of phase-separated biomolecular condensates, specifically stress granules, in neurodegenerative diseases like ALS. ALS-associated mutations in genes involved in stress granule assembly have been found, and stress granule proteins have been detected in pathological inclusions in ALS patient neurons. However, protein components of stress granules are also present in other physiological biomolecular condensates, which have not been adequately discussed in relation to ALS. This review explores the functions of TDP-43 and FUS in physiological condensates occurring in the nucleus and neurites beyond stress granules, and discusses the impact of ALS-linked mutations on their ability to phase separate and perform their functions in stress-independent biomolecular condensates.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Alexander Lin, Yogambha Ramaswamy, Ashish Misra
Summary: Smooth muscle cells, endothelial cells, and macrophages in blood vessels display remarkable heterogeneity, and their developmental origins may influence their plasticity. Unbiased single cell whole transcriptome analysis techniques are revolutionizing the understanding of cellular diversity and plasticity, providing insights for therapeutic research.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Elton P. Hudson
Summary: The Calvin Benson cycle plays a crucial role in the ecological and biotechnological aspects of bacteria. Recent studies have shed light on the regulation of this cycle in bacteria, with post-transcriptional and post-translational regulation being important in phototrophic bacteria, and transcriptional regulation being prominent in chemolithoautotrophic bacteria. Understanding the regulation of the Calvin Benson cycle has implications for enhancing CO2 fixation and improving the synthesis of desired products. Non-canonical cycles may offer potential benefits for industrial applications.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Xin-Guang Zhu, Haim Treves, Honglong Zhao
Summary: This paper discusses the major regulatory mechanisms over the Calvin Benson Cycle (CBC) that maintain homeostasis of metabolite levels. These mechanisms include redox regulation of enzymes, metabolite regulations (especially allosteric regulations), and balanced activities of enzymes. These regulatory mechanisms are crucial for maintaining high flux and photosynthetic efficiency in CBC.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Hunter C. Herriage, Yi-Ting Huang, Brian R. Calvi
Summary: Apoptosis prevents the inappropriate acquisition of extra copies of the genome, known as polyploidy, but the polyploid state can suppress apoptosis. The mechanisms linking apoptosis and polyploid cell cycles are still largely unknown, and studying the regulation of apoptosis in development and cancer could lead to more effective therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Daniel Campbell, Steven Zuryn
Summary: Mitochondrial dynamics play a crucial role in regulating cellular and organismal homeostasis, impacting various aspects of an organism's healthspan. By studying the nematode Caenorhabditis elegans, a comprehensive understanding of the impact of mitochondrial dynamics on homeostasis over a lifetime can be obtained.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Diba Borgmann, Henning Fenselau
Summary: Maintaining blood glucose at an appropriate physiological level requires coordination of multiple organs and tissues, with the vagus nerve playing a key role in central control. Recent studies have revealed the cellular identity, neuroanatomical organization, and functional contributions of vagal neurons in the regulation of systemic glucose metabolism. These findings provide new insights into the precise roles of vagal neurons in coordinating glucose levels and offer potential avenues for treating glucose metabolism disorders.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)
Review
Cell Biology
Tatiana C. Coverdell, Stephen B. G. Abbott, John N. Campbell
Summary: In this article, we review how genetic technology and single-cell genomics are revealing the organizational principles of the efferent vagus in unprecedented detail.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2024)