Article
Cell Biology
Jennifer Imm, Ehsan Pishva, Muhammadd Ali, Talitha L. Kerrigan, Aaron Jeffries, Joe Burrage, Enrico Glaab, Emma L. Cope, Kimberley M. Jones, Nicholas D. Allen, Katie Lunnon
Summary: This study investigated dynamic changes in DNA methylation during neuronal differentiation using induced pluripotent stem cell-based models. Significant loci with progressive alterations in DNA methylation were identified during the differentiation process. A network analysis revealed a set of densely connected genes, with STAT3 showing the highest connectivity and influencing neuronal differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Article
Cell Biology
Ivan N. Vlasov, Anelya Kh. Alieva, Ekaterina V. Novosadova, Elena L. Arsenyeva, Anna V. Rosinskaya, Suzanna A. Partevian, Igor A. Grivennikov, Maria I. Shadrina
Summary: Parkinson's Disease is a severe neurodegenerative disease characterized by a deficiency of the dopaminergic system. While genetic factors play a significant role in the pathogenesis of PD, gene expression changes may also be important. Research suggests that TNF may play a crucial role in PD-related changes, with INHBA, WNT7A, and DKK1 identified as possible downstream effectors of TNF.
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Karo Talvio, Rimante Minkeviciene, Kayla G. Townsley, Venkat Swaroop Achuta, Laura M. Huckins, Padraic Corcoran, Kristen J. Brennand, Maija L. Castren
Summary: This study compared the transcriptomes of human neural progenitors (NPCs) generated from patient-derived induced pluripotent stem cells (iPSCs) of three fragile X syndrome (FXS) and three control male donors. The results showed altered expression of several genes related to triplet repeat instability, RNA splicing, testes development, and pathways previously shown to be affected in FXS. The study also revealed disturbed growth linked to reduced expression of LYNX1, suggesting dysregulated cholinergic system. The findings highlight the potential of human iPSCs in disease modeling and therapeutic interventions for FXS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lital Gildin, Rossana Rauti, Ofir Vardi, Liron Kuznitsov-Yanovsky, Ben M. Maoz, Menahem Segal, Dalit Ben-Yosef
Summary: Fragile X syndrome affects the development and function of human neuronal networks, with smaller cell bodies and reduced connections observed in FX-iNs compared to control iNs. FX networks also exhibit higher spontaneous burst-firing activity and lower network synchrony, providing insight into the intellectual dysfunction associated with FXS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hui Huang, Soujanya S. Karanth, Ya Guan, Sebastian Freeman, Ryan Soron, David S. Godovich, Jianjun Guan, Kaiming Ye, Sha Jin
Summary: This study utilizes oxygen-releasing biomaterials and an oxygenated scaffold culture platform to enhance oxygen supply during 3D iPSC pancreatic differentiation. The oxygenation does not affect the scaffold's mechanical properties, but improves oxygen tension within the scaffolds. The unique 3D differentiation system enables the generation of islet organoids with enhanced expression of islet signature genes and proteins, as well as improved sensitivity to glucose for insulin secretion.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Oncology
A. Pham, C. Selenou, E. Giabicani, V. Fontaine, S. Marteau, F. Brioude, L. David, D. Mitanchez, M. L. Sobrier, I. Netchine
Summary: This study extensively analyzed the methylation levels of imprinted control regions (ICRs) in iPSCs and found abnormal hypermethylation at certain ICRs, resulting in loss of parental imprinting (LOI) and biallelic expression of imprinted genes. They also discovered that the epiPS (TM) culture medium and culturing iPSCs under hypoxic conditions prevented hypermethylation at ICRs while preserving the properties of iPSCs. This suggests that human iPSCs are a promising cellular model to study the physiopathology of imprinting disorders (IDs) and test therapeutic strategies.
CLINICAL EPIGENETICS
(2022)
Article
Cell Biology
Alexandra Neaverson, Malin H. L. Andersson, Osama A. Arshad, Luke Foulser, Mary Goodwin-Trotman, Adam Hunter, Ben Newman, Minal Patel, Charlotte Roth, Tristan Thwaites, Helena Kilpinen, Matthew E. Hurles, Andrew Day, Sebastian S. Gerety
Summary: Efficient and effective methods for converting human induced pluripotent stem cells into differentiated derivatives are critical for performing robust, large-scale studies of development and disease modelling, and for providing a source of cells for regenerative medicine. Here, we describe a 14-day neural differentiation protocol which allows for the scalable, simultaneous differentiation of multiple iPSC lines into cortical neural stem cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Shuo Cao, Xinyue Gao, Fangyuan Liu, Yanglin Chen, Qin Na, Qiaoqiao Meng, Peng Shao, Chen Chen, Yongli Song, Baojiang Wu, Xihe Li, Siqin Bao
Summary: This study derived iPSCs from fibroblasts of a patient with acute myelitis and compared them to iPSCs from normal individuals. The patient-derived iPSCs showed attenuated potential for ectoderm differentiation, which was found to be related to the Wnt signaling pathway. This study provides a novel approach to investigating the pathogenesis of acute myelitis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Hematology
Haiyun Pei, Huilin Li, Lei Xu, Bowen Zhang, Heng Zhang, Yali Jia, Liqing Liang, Xiaoyan Xie, Zeng Fan, Zhou Yang, Xiaoling Wang, Feiling Song, Lijuan He, Wen Yue, Xuetao Pei
Summary: The major challenge in using human induced pluripotent stem cells (hiPSCs) is their efficient and controlled differentiation into specific cell types. This study focused on understanding the characteristics of hiPSCs derived from human umbilical arterial endothelial cells (HuA-iPSCs) and their differentiation potential. It was found that HuA-iPSCs retained a transcriptional memory and DNA methylation signature similar to their parental cells, and they showed the highest efficiency in differentiating into hematopoietic cells compared to other hiPSCs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Alexander Bluemke, Erica Ijeoma, Jessica Simon, Rachel Wellington, Medania Purwaningrum, Sergei Doulatov, Elizabeth Leber, Marta Scatena, Cecilia M. Giachelli
Summary: This study compared the ability of two different iPSC lines (PBMC-derived and fibroblast-derived) and two differentiation protocols (embryoid body-based and monolayer-based) to differentiate into osteoclasts. The results showed that embryoid body-based differentiation was successful in generating osteoclasts, while monolayer-based differentiation was not. There was no difference in osteoclast differentiation between the two iPSC lines.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Medicine, Research & Experimental
Ping Lu, Guang Yang, Lichun Jiang, Wen He, Wanwan Wu, Lingbin Qi, Shijun Shen, Junhua Rao, Peng Zhang, Zhigang Xue, Cizhong Jiang, Guoping Fan, Xianmin Zhu
Summary: This study found that Leptin mutant rats exhibit NASH phenotypes and observed NASH progression through hepatic transcriptome analysis. The research revealed that Leptin-deficient rats bear more similarities to human NASH patients, providing a valuable pre-clinical rodent model for evaluating NASH drug safety and efficacy.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Environmental Sciences
Shibiao Bai, Ping Lu
Summary: This study investigated two types of growth disturbances in tree-ring sequences in China, revealing nine reactivation phases of a landslide over the past 30 years. Hazard analysis showed that the probability of landslide reactivation in the next 20 years was highest in the upper part of the landslide and on the boundaries of new and old landslides, while the lower landslide body was more stable. The research demonstrated the usefulness of dendrochronology in reconstructing modern reactivations of a large colluvial landslide.
LAND DEGRADATION & DEVELOPMENT
(2021)
Article
Engineering, Geological
Ping Lu, Wenyang Shi, Qunming Wang, Zhongbin Li, Yuanyuan Qin, Xuanmei Fan
Summary: The study incorporated Sentinel-2 10-m fused NIR narrow, red-edge-1, SWIR-1, and SWIR-2 bands into change detection-based Markov random field (CDMRF) for co-seismic landslide mapping. The results showed that the mapping performances with the fused Sentinel-2 bands were significantly improved for both seismic events. The study highlighted the novelty of using fused Sentinel-2 bands and the synergy of red-edge, NIR narrow, and SWIR bands for more reliable landslide mapping.
Article
Oncology
Qing Huang, Changchun Chen, Sheng Hu, Huijing Wu, Ding Yu, Xianmin Zhu, Chang Xue, Yuebing Wu, Jing Tang, Rong Xie, Fengming Ran
Summary: We report a rare case of lung adenocarcinoma with synchronous EGFR G719A and BRAF V600E mutations, in which the patient had a long-term response to afatinib, highlighting its efficacy as a therapeutic option for such patients.
Article
Geochemistry & Geophysics
Ping Lu, Wenyang Shi, Zhongbin Li
Summary: This study demonstrates the suitability and potential of low-orbit miniature satellites like PlanetScope for rapid mapping of landslide inventory. By combining change detection methods with the region-based level set evolution (RLSE) method, the efficiency and accuracy of landslide mapping can be improved.
IEEE GEOSCIENCE AND REMOTE SENSING LETTERS
(2022)
Article
Oncology
Shuang Dong, Wuling Ou, Yi Zhong, Xianmin Zhu, Qian Cai, Jing Zhang, Fengming Ran, Yu Qian, Jun Wang, Sheng Hu
Summary: The combination therapy of apatinib and pemetrexed shows promising efficacy and high safety profile in advanced NSCLC patients, highlighting the need for further studies to evaluate its effectiveness and safety.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Geography, Physical
Davide Festa, Manuela Bonano, Nicola Casagli, Pierluigi Confuorto, Claudio De Luca, Matteo Del Soldato, Riccardo Lanari, Ping Lu, Michele Manunta, Mariarosaria Manzo, Giovanni Onorato, Federico Raspini, Ivana Zinno, Francesco Casu
Summary: This study utilizes satellite-based multi-temporal interferometric datasets to investigate terrain changes in Italy, and classifies triggering factors, showing that landslides and subsidence events are the main causes of terrain deformation.
ISPRS JOURNAL OF PHOTOGRAMMETRY AND REMOTE SENSING
(2022)
Article
Oncology
Jing Tang, Xuqian Ma, Xin Chen, Mingqian Feng, Xin Jin, Ke Wang, Jixi Wan, Xianmin Zhu, Rong Xie, Shuang Dong, Sheng Hu, Hui Jiang, Conghua Xie
Summary: This study investigated the potential of B7-H4-targeted immunotherapy for breast cancer. Four monoclonal antibodies against B7-H4 were generated and used to create T cell-engaged bispecific antibodies and immunotoxins. In vitro cytotoxicity assays showed that both bispecific antibodies and immunotoxins were toxic to B7-H4 positive cells, with the former being more potent. In vivo studies revealed that immunotoxin was slightly more effective in suppressing tumor growth. Furthermore, the combination of bispecific antibody and oncolytic virus HSV-2 showed a synergistic effect in suppressing tumor growth without causing obvious adverse effects. These findings suggest a novel strategy for enhancing antitumor immune response.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Environmental Sciences
Qi Liu, Jie Niu, Ping Lu, Feifei Dong, Fujun Zhou, Xianglian Meng, Wei Xu, Shan Li, Bill X. Hu
Summary: This study compared the performance of machine learning (ML) and deep learning (DL) methods in estimating the active layer thickness (ALT) and seasonal thaw depth of permafrost on the Qinghai-Tibetan Plateau. The results showed that convolutional neural networks (CNN) and long short-term memory (LSTM) models developed with longer lagging times exhibited better performance in thaw depth prediction compared to the randomforest (RF) models. The study also found an increasing trend in ALT on the Qinghai-Tibetan Plateau from 2003 to 2011, especially in the northern region. Additionally, the seasonal thaw depth deepened in different seasons.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Oncology
Xianmin Zhu, Shuang Dong, Jing Tang, Rong Xie, Huijing Wu, Paul Hofman, Maciej M. Mrugala, Sheng Hu
Summary: This case report suggests that immunotherapy combined with TMZ may be an effective and safe therapeutic option for untreated BM in NSCLC patients. Although the results are encouraging, further research is needed to confirm the synergy observed.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Geochemistry & Geophysics
Ping Lu, Jiangping Han, Yonghong Yi, Tong Hao, Fujun Zhou, Xianglian Meng, Yinsheng Zhang, Rongxing Li
Summary: This study utilized InSAR technology to monitor the dynamic changes in permafrost in the Hoh Xil region on the Tibetan Plateau. The results show that the permafrost in this region is experiencing disturbances, which are closely related to changes in land surface temperature. Thawing permafrost has led to the formation and evolution of thermokarst landforms, such as retrogressive thaw slumps and thermokarst lakes.
IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING
(2023)
Article
Geochemistry & Geophysics
Chengyuan Zhang, Qunming Wang, Ping Lu, Yong Ge, Peter M. Atkinson
Summary: This article proposes a fast and slow changes constrained spatio-temporal subpixel mapping (FSSTSPM) method to enhance subpixel mapping (SPM) by utilizing temporal information from time-series images. The FSSTSPM method is validated using synthetic datasets and real datasets, and the results demonstrate its superiority, especially in the presence of proportion errors.
IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING
(2022)
Article
Engineering, Electrical & Electronic
Wenyang Shi, Ping Lu
Summary: This study utilized multiple methods to efficiently and rapidly perceive geological hazards along the Sichuan-Tibet Railway, providing important references for railway construction. The results showed that the combination of ICA and MRF performed more completely and accurately in extracting landslides.
IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING
(2021)
Article
Biochemistry & Molecular Biology
Di He, Di Wang, Ping Lu, Nan Yang, Zhigang Xue, Xianmin Zhu, Peng Zhang, Guoping Fan
Summary: The study revealed significant heterogeneity within early-stage LUAD carrying EGFR mutations, indicating complex interactions among tumor cells, stromal cells and immune infiltrates in the TME.