期刊
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
卷 17, 期 1, 页码 753-759出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14686996.2016.1243000
关键词
DNA; G-quadruplex; duplex; molecular crowding; osomolyte
资金
- MEXT, Japan [KAKENHI 15H03840, 16K14042]
- JSPS, Japan [2306]
- Asahi Glass Foundation
- Grants-in-Aid for Scientific Research [16K14042, 15H03840, 15H01807, 16H01692] Funding Source: KAKEN
We systematically investigated effects of molecular crowding with trimethylamine N-oxide (TMAO) as a zwitterionic and protective osmolyte and urea as a nonionic denaturing osmolyte on conformation and thermodynamics of the canonical DNA duplex and the non-canonical DNA G-quadruplex. It was found that TMAO and urea stabilized and destabilized, respectively, the G-quadruplex. On the other hand, these osmolytes generally destabilize the duplex; however, it was observed that osmolytes having the trimethylamine group stabilized the duplex at the lower concentrations because of a direct binding to a groove of the duplex. These results are useful not only to predict DNA structures and their thermodynamics under physiological environments in living cells, but also design of polymers and materials to regulate structure and stability of DNA sequences.
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