Article
Dermatology
Gopal Chovatiya, Sangeeta Ghuwalewala, Lauren D. Walter, Benjamin D. Cosgrove, Tudorita Tumbar
Summary: This study investigates the molecular heterogeneity of hair follicle stem cells (HFSCs) using high-resolution single-cell RNA sequencing and identifies five major populations distinguished by known markers associated with the bulge and outer root sheath (ORS). Lineage trajectory analysis predicts prospective differentiation paths of cellular states within these populations. Gene set enrichment analysis reveals potential distinct functions of different subpopulations within the bulge and ORS, shedding light on communication and nutrient transfer mechanisms within the hair follicle.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Cell & Tissue Engineering
Yuyang Gan, Hailin Wang, Lijuan Du, Zhexiang Fan, Pingping Sun, Kaitao Li, Qian Qu, Jin Wang, Ruosi Chen, Zhiqi Hu, Yong Miao
Summary: A large number of pelage HF-MSCs can be quickly and conveniently isolated from adult mice using a two-step Ficoll density gradient sedimentation method. These cells possess similar characteristics and functions as whisker HF-MSCs and can promote hair growth by secreting exosomes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Veterinary Sciences
Wei Yan, Fei Hao, Xiaoshu Zhe, Yingmin Wang, Dongjun Liu
Summary: This study confirmed the biological characteristics and differentiation potential of PHFSCs and SHFSCs from Arbas Cashmere goats, expanding and refining our knowledge of adult stem cell types and characteristics.
BMC VETERINARY RESEARCH
(2022)
Article
Oncology
Hui Cheng, Fei Liu, Manqian Zhou, Shang Chen, Haoyan Huang, Yue Liu, Xiaotong Zhao, Qiaonan Zhang, Xinrun Zhou, Zongjin Li, Hong Cai
Summary: The study found that the PGE2 matrix can promote early stage hair follicle development and improve the condition of hair follicle stem cells. In addition, the PGE2 delivery system may be a new strategy for treating hair disorders.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Dermatology
Wen-Yen Huang, Jin-Bon Hong, Michael Chang, Shih-Yi Wang, Shih-Fan Lai, Hsiung-Fei Chien, Sung-Jan Lin
Summary: The effects of cyclophosphamide on anagen hair follicles depend on dosage, with low dosages leading to dystrophy that can be self-repaired, while high dosages prematurely induce other growth stages. Analysis of cell dynamics revealed that apoptosis of central cells in hair bulbs is repairable, bulge stem cells remain unaffected, and basal cup cells can quickly replenish damaged cells and reconstruct structures.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Cell Biology
Michiko Yamane, Nanako Takaoka, Koya Obara, Kyoumi Shirai, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Robert M. Hoffman, Yasuyuki Amoh
Summary: Hair-follicle-associated pluripotent (HAP) stem cells, located in hair follicles, can differentiate into multiple cell types, including neurons and cardiomyocytes. Studies have shown that HAP stem cells treated with neural-induction medium can differentiate into dopaminergic neurons, which may potentially help improve symptoms of Parkinson's disease in the future.
Article
Cell & Tissue Engineering
Eva Maria Rico-Leo, Luis Francisco Lorenzo-Martin, Angel Carlos Roman, Xose Ramon Bustelo, Jaime Maria Merino, Pedro Maria Fernandez-Salguero
Summary: The aryl hydrocarbon receptor (AhR) plays a crucial role in skin regeneration and homeostasis by maintaining the identity of epidermal stem cells, making it a potential target for treating cutaneous pathologies.
Article
Materials Science, Biomaterials
Sugi Hirano, Tatsuto Kageyama, Maki Yamanouchi, Lei Yan, Kohei Suzuki, Katsumi Ebisawa, Keiichiro Kasai, Junji Fukuda
Summary: Hair regeneration using HFSCs and dermal papilla cells has potential for alopecia treatment. This study developed a novel approach for HFSC expansion through cell aggregates formation and Matrigel encapsulation. The HFSCs cultured using a microarray device showed a 20-fold increase in quantity and higher expression of trichogenic stem cell markers compared to conventional culture methods. HFSCs cultured using this method combined with embryonic mesenchymal cells demonstrated vigorous hair regeneration in nude mice. Moreover, the expansion of human HFSCs from androgenetic alopecia patients showed significant improvement using this approach. Therefore, this study proposes a useful method for HFSC expansion in hair regenerative medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Integrative & Complementary Medicine
Xiaojiao Fan, Jing Chen, Yajun Zhang, Siyi Wang, Wenqian Zhong, Huipu Yuan, Xia Wu, Chaochen Wang, Yixin Zheng, Yuan Wei, Ying Xiao
Summary: This study clarified the mechanism by which Alpinetin (AP) promotes hair regeneration by activating HFSCs via Wnt signaling. These findings may contribute to the development of a new generation of pilatory that is more efficient and less cytotoxic for treating alopecia.
Review
Cell Biology
Sangbum Park
Summary: Hair follicles undergo continuous growth and regression cycles driven by the regulation of stem cells and niche components. Complex morphogenesis is required to build the structure of hair follicles during embryonic development. Understanding the formation and maintenance of hair follicles, as well as the mechanisms for hair follicle generation and skin regeneration, is crucial.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jingjing Wang, Yuheng Fu, Wenmao Huang, Ritusree Biswas, Avinanda Banerjee, Joshua A. Broussard, Zhihai Zhao, Dongmei Wang, Glen Bjerke, Srikala Raghavan, Jie Yan, Kathleen J. Green, Rui Yi
Summary: This study reveals that hair follicle stem cells (SCs) and hair germ (HG) progenitors have different mechanical properties, with SCs being stiff and resistant to size change, while HG progenitors are soft and periodically enlarge and contract. During hair follicle growth, HGs reduce contraction and more frequently enlarge, which is associated with weakening of the actomyosin network and nuclear YAP accumulation. miR-205 can reduce actomyosin contractility and activate hair regeneration in mice.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Kefei Nina Li, Tudorita Tumbar
Summary: Stem cells, particularly hair follicle stem cells, actively communicate with their microenvironment by sending out signals; the reconstruction of stem cell niches is crucial for skin homeostasis and regeneration; the interaction between hair follicle stem cells and their niches provides important insights into skin biology.
Article
Biology
Sisi Zhang, Rachel W. S. Chan, Ernest H. Y. Ng, William S. B. Yeung
Summary: Notch signaling plays a key role in maintaining the quiescent state of endometrial mesenchymal stromal/stem cells (eMSC), and is influenced by Notch and Wnt signaling from the microenvironment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemical Research Methods
Allen W. Lynch, Christina Theodoris, Henry Long, Myles Brown, X. Shirley Liu, Clifford A. Meyer
Summary: MIRA is a comprehensive methodology that compares gene expression and chromatin accessibility in single cells to infer regulatory mechanisms driving cell fate transitions. It models cell states and regulatory potential of gene loci to represent cell states in an efficient and interpretable latent space, and reveals the variable influence of local accessibility on transcription at different gene loci.
Article
Multidisciplinary Sciences
Carla M. Abreu, Mariana T. Cerqueira, Rogerio P. Pirraco, Luca Gasperini, Rui L. Reis, Alexandra P. Marques
Summary: The conditioned medium from interfollicular keratinocytes (KCs-CM) has been shown to improve the hair inductive ability of DP cells, with significant effects on DP cell growth and restoration of activity. Additionally, DP cells cultured with KCs-CM in spheroids demonstrated superior activity and supported the formation of hair follicle- and sebaceous gland-like structures in mice when co-grafted with human keratinocytes.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Glen A. Bjerke, Rui Yi
Article
Multidisciplinary Sciences
Jingjing Wang, Jerome E. Lee, Kent Riemondy, Yang Yu, Steven M. Marquez, Eric C. Lai, Rui Yi
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Daniel T. Youmans, Anne R. Gooding, Robin D. Dowell, Thomas R. Cech
Summary: Mutations in the SUZ12 subunit of PRC2 can drive it into PRC2.1 or PRC2.2 subcomplexes, affecting the expression of certain genes in pluripotent stem cells, particularly homeobox transcription factors.
Article
Medicine, Research & Experimental
Fabienne Gally, Sarah K. Sasse, Jonathan S. Kurche, Margaret A. Gruca, Jonathan H. Cardwell, Tsukasa Okamoto, Hong W. Chu, Xiaomeng Hou, Olivier B. Poirion, Justin Buchanan, Sebastian Preissl, Bing Ren, Sean P. Colgan, Robin D. Dowell, Ivana Yang, David A. Schwartz, Anthony N. Gerber
Summary: The G/T transversion rs35705950, located 3 kb upstream of the MUC5B start site, is identified as a key risk factor for idiopathic pulmonary fibrosis. Through analysis of accessible chromatin and nuclease sensitivity data, it is shown that this region affects the expression of MUC5B and is subject to epigenetic remodeling in IPF patients' lung tissue.
Article
Multidisciplinary Sciences
Qing Yang, Tassa K. Saldi, Patrick K. Gonzales, Erika Lasda, Carolyn J. Decker, Kimngan L. Tat, Morgan R. Fink, Cole R. Hager, Jack C. Davis, Christopher D. Ozeroff, Denise Muhlrad, Stephen K. Clark, Will T. Fattor, Nicholas R. Meyerson, Camille L. Paige, Alison R. Gilchrist, Arturo Barbachano-Guerrero, Emma R. Worden-Sapper, Sharon S. Wu, Gloria R. Brisson, Matthew B. McQueen, Robin D. Dowell, Leslie Leinwand, Roy Parker, Sara L. Sawyer
Summary: The study found that even asymptomatic individuals with SARS-CoV-2 may have low viral loads that are not infectious. Moreover, only 2% of individuals are responsible for carrying 90% of the virions circulating within communities, potentially acting as viral supercarriers and superspreaders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Marko Melnick, Patrick Gonzales, Thomas J. LaRocca, Yuping Song, Joanne Wuu, Michael Benatar, Bjorn Oskarsson, Leonard Petrucelli, Robin D. Dowell, Christopher D. Link, Mercedes Prudencio
Summary: This study developed a bioinformatic pipeline to identify potential etiological agents in RNA-seq data, successfully confirming the presence of known and novel viral sequences through testing and application in practical cases.
G3-GENES GENOMES GENETICS
(2021)
Article
Engineering, Biomedical
Cierra J. Walker, Claudia Crocini, Daniel Ramirez, Anouk R. Killaars, Joseph C. Grim, Brian A. Aguado, Kyle Clark, Mary A. Allen, Robin D. Dowell, Leslie A. Leinwand, Kristi S. Anseth
Summary: Increased extracellular stiffness activates fibroblasts, leading to the transformation of transiently activated fibroblasts into myofibroblasts with condensed chromatin. The condensed chromatin structure of myofibroblasts is associated with cytoskeletal stability, and nuclear mechanosensing plays a key role in driving distinct chromatin signatures in persistently activated fibroblasts.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Immunology
Lara N. Janiszewski, Michael Minson, Mary A. Allen, Robin D. Dowell, Amy E. Palmer
Summary: The study shows that host cells undergoing Salmonella infection undergo widespread changes in metal transport, metal-dependent genes, and metal homeostasis genes to remodel iron, copper, and zinc availability. Infection increases cytosolic zinc levels while limiting zinc within phagosomes. Manipulation of zinc at the host-pathogen interface is more nuanced than that of iron or copper.
INFECTION AND IMMUNITY
(2021)
Article
Biochemistry & Molecular Biology
Arnav Gupta, Sarah K. Sasse, Margaret A. Gruca, Lynn Sanford, Robin D. Dowell, Anthony N. Gerber
Summary: This study employed precision nuclear run-on sequencing and assay for transposase-accessible chromatin sequencing to investigate the effects of wood smoke particles on airway epithelial cells. It found a primary role for AHR in mediating the response to WSP in airway epithelial cells, as well as crosstalk between AHR and NF kappa B signaling in controlling proinflammatory gene expression.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Cecilia B. Levandowski, Taylor Jones, Margaret Gruca, Sivapriya Ramamoorthy, Robin D. Dowell, Dylan J. Taatjes
Summary: This study investigated the impact of the interaction between Delta 40p53 and WTp53 on growth arrest and mRNA biogenesis. The results showed that Delta 40p53:WTp53 can influence cellular response to Nutlin-induced p53 activation, as well as specific changes in mRNA induction in response to other stimuli.
Article
Biology
Jonathan D. Rubin, Jacob T. Stanley, Rutendo F. Sigauke, Cecilia B. Levandowski, Zachary L. Maas, Jessica Westfall, Dylan J. Taatjes, Robin D. Dowell
Summary: Transcription Factor Enrichment Analysis (TFEA) is a computational method that detects positional motif enrichment associated with changes in transcription, providing insights into cellular processes. TFEA is applicable to various types of transcriptional regulation data, identifying key regulators and unraveling temporal regulatory networks.
COMMUNICATIONS BIOLOGY
(2021)
Editorial Material
Cell Biology
Rui Yi, Yali Dou
Summary: A study uncovers that Pioneer transcription factors can bind closed chromatin regions, modify local accessibility, and activate target genes. The SOX9 transcription factor drives cell fate switching by activating hair follicle cell enhancers, while simultaneously repressing epidermal enhancers through sequestration of epigenetic factors.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Dongmei Wang, Haimin Li, Navdeep S. Chandel, Yali Dou, Rui Yi
Summary: This study reveals the important roles of MOF and H4K16ac in controlling cellular function and regulating mammalian tissue development. Conditional deletion of Mof in the skin causes severe defects in the self-renewal of basal epithelial progenitors, epidermal differentiation, and hair follicle growth, resulting in barrier defects and perinatal lethality. The study also highlights the essential functions of MOF-regulated genes in mitochondria and cilia.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Chi Zhang, Dongmei Wang, Jingjing Wang, Li Wang, Wenli Qiu, Tsutomu Kume, Robin Dowell, Rui Yi
Summary: This study reveals that epithelial cells escaping from their niche in aged tissues contribute to stem cell reduction and tissue degeneration, leading to hair follicle miniaturization. The reduced expression of cell adhesion and extracellular matrix genes in aged hair follicle stem cells regulated by Foxc1 and Nfatc1 is a key factor in this process. Live imaging captures the migration of individual epithelial cells away from the stem cell compartment, illustrating a previously unknown mechanism of tissue deterioration during aging.
Review
Biochemistry & Molecular Biology
Joseph F. Cardiello, Gilson J. Sanchez, Mary A. Allen, Robin D. Dowell
TRANSCRIPTION-AUSTIN
(2020)
