期刊
RNA BIOLOGY
卷 14, 期 1, 页码 20-28出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1251541
关键词
Nascent RNA; neuronal RNA; newly synthesized RNA; PABP; RNA in zebrafish brain; visualization of RNA; Y10B
资金
- Max Planck Society
- European Research Council
- DFG CRC [1080, 902]
- DFG Cluster of Excellence for Macromolecular Complexes
The neuronal transcriptome changes dynamically to adapt to stimuli from the extracellular and intracellular environment. In this study, we adapted for the first time a click chemistry technique to label the newly synthesized RNA in cultured hippocampal neurons and intact larval zebrafish brain. Ethynyl uridine (EU) was incorporated into neuronal RNA in a time- and concentration-dependent manner. Newly synthesized RNA granules observed throughout the dendrites were colocalized with mRNA and rRNA markers. In zebrafish larvae, the application of EU to the swim water resulted in uptake and labeling throughout the brain. Using a GABA receptor antagonist, PTZ (pentylenetetrazol), to elevate neuronal activity, we demonstrate that newly transcribed RNA signal increased in specific regions involved in neurogenesis.
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