期刊
CHROMOSOME RESEARCH
卷 23, 期 4, 页码 767-779出版社
SPRINGER
DOI: 10.1007/s10577-015-9495-3
关键词
Centromere; CENP-A; ChIP-seq; Gasterosteus aculeatus; Threespine stickleback
资金
- National Science Foundation [DGE-1256082]
- National Institutes of Health Chromosome Metabolism and Cancer Training Grant [T32 CA009657]
- Fred Hutchinson Cancer Research Center
Centromere sequences exist as gaps in many genome assemblies due to their repetitive nature. Here we take an unbiased approach utilizing centromere protein A (CENP-A) chomatin immunoprecipitation followed by high-throughput sequencing to identify the centromeric repeat sequence in the threespine stickleback fish (Gasterosteus aculeatus). A 186-bp, AT-rich repeat was validated as centromeric using both fluorescence in situ hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This repeat hybridizes strongly to the centromere on all chromosomes, with the exception of weak hybridization to the Y chromosome. Together, our work provides the first validated sequence information for the threespine stickleback centromere.
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