Article
Biochemistry & Molecular Biology
Suphagan Boonpethkaew, Jitlada Meephansan, Onjira Jumlongpim, Pattarin Tangtanatakul, Wipasiri Soonthornchai, Jongkonnee Wongpiyabovorn, Ratchanee Vipanurat, Mayumi Komine
Summary: Exploring the transcriptome in the peripheral edge of lesional skin is crucial for understanding the molecules and signaling pathways that contribute to inflammation. The study identified differentially expressed genes in the lesional skin, which enhanced functions related to tissue growth, cell migration, and immune response. The findings suggest that inflammation in the lesional skin may be driven by specific signaling pathways, potentially leading to the discovery of new therapeutic targets for psoriasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chih-Chieh Chen, Yung-Che Chou, Chia-Yi Hsu, Eing-Mei Tsai, Tze-Kiong Er
Summary: In this study, RNA sequencing analysis was used to identify a potential pathogenic gene of endometriosis. The differentially expressed genes were found to be enriched in several signaling pathways, providing new insights into the underlying mechanisms of endometriosis.
Review
Immunology
Peng Zhang, Yuwen Su, Siying Li, Hui Chen, Ruifang Wu, Haijing Wu
Summary: This paper reviews the different subtypes of T lymphocytes and their functions in psoriasis, as well as provides an overview of targeted therapies for treating psoriasis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Otto Seppaelae, Jean-Claude Walser, Teo Cereghetti, Katri Seppaelae, Tiina Salo, Coen M. Adema
Summary: This study used RNA sequencing to characterize the transcriptome profiles of snails, identifying immune defense factors and showing high among-individual variation in response to immune stimuli and environmental conditions. These findings suggest the importance of including factors with high individual variation in future ecoimmunological studies to understand differences in parasite resistance among natural snail populations.
Article
Psychiatry
William W. Eaton, Katrina M. Rodriguez, Mekha A. Thomas, Jeanette Johnson, Monica V. Talor, Curtis Dohan, Clifton O. Bingham III, Rashelle Musci, Kimberly Roth, Deanna L. Kelly, Daniela Cihakova, Erika Darrah
Summary: The relationship between schizophrenia and rheumatoid arthritis has been observed for 85 years, but the mechanisms driving this association are unknown. This study analyzed differences in cytokines, selected genes, and antibodies related to gluten sensitivity, celiac disease, and systemic autoimmunity in patients with schizophrenia, rheumatoid arthritis, and healthy controls. The analysis revealed potential subgroups within schizophrenia patients based on their cytokine profiles, providing new directions for treatment strategies.
PSYCHIATRY RESEARCH
(2022)
Article
Medicine, General & Internal
Magdalena Kutwin, Monika Migdalska-Sek, Ewa Brzezianska-Lasota, Piotr Zelga, Anna Wozniacka
Summary: Psoriasis is an immune disease characterized by elevated expression of IL-17A and IL-23R in the skin of patients, while IL-10 expression is lower. The study found a positive correlation between IL-23A expression levels and PASI index in psoriasis patients. Further research is needed to understand the exact role of these cytokines in the pathogenesis of psoriasis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Dermatology
Pinyadapat Vacharanukrauh, Jitlada Meephansan, Saranyoo Ponnikorn, Pattarin Tangtanatakul, Wipasiri Soonthornchai, Jongkonnee Wongpiyabovorn, Patlada Ingkaninanda, Akimichi Morita
Summary: Through RNA sequencing and pathway analysis, the study found that NB-UVB treatment may improve psoriasis by suppressing NF-κB, enhancing sirtuin signaling pathway, and decreasing the function of key upstream regulators associated with proinflammatory cytokines. The study also highlighted the gene expression changes associated with the modulation of autoinflammation after NB-UVB treatment.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Himani Punia, Jayanti Tokas, Anurag Malik, Sonali Sangwan, Anju Rani, Shikha Yashveer, Saleh Alansi, Maha J. Hashim, Mohamed A. El-Sheikh
Summary: Salinity stress is a major concern for global food security, and understanding the mechanisms that enable plants to survive under salt stress is crucial. Sorghum has become a focus of research in this area, with a systematic analysis of differentially expressed genes and their functions revealing involvement in important metabolic processes under high salinity. The findings highlight the potential for developing salt-resistant sorghum lines by targeting genes involved in response to salinity.
Article
Veterinary Sciences
Yameng Wei, Kangle Yi, Caomeihui Shen, Xue Chen, Tariq Iqbal, Maosheng Cao, Tong Chen, Yang Luo, Jianbo Li, Xu Zhou, Chunjin Li, Lu Chen
Summary: In this study, the toxic effects of cadmium on the liver of Xiangxi heifers were investigated using whole-transcriptome sequencing. The results showed that cadmium exposure led to differential expression of genes involved in autophagy regulation, apoptosis, lipid metabolism, anti-inflammation, and antioxidant enzyme activity. These findings provide valuable biomarkers for predicting and treating cadmium-related diseases in the future.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Timothy J. Liu, Lynlee L. Lin, Erin McMeniman, Jason Wu, Yung-Ching Kao, Snehlata Kumari, Glen M. Boyle, James W. Wells, H. Peter Soyer, Jazmina L. Gonzalez-Cruz
Summary: In this pilot study, the possibility of diagnosing inflammatory skin diseases from an immunological perspective was explored. Significant differences in the expression of inflammatory markers were detected between lesional and perilesional biopsies, indicating the potential of inflammatory signatures as diagnostic differentiators and alternative targets in anti-inflammatory treatment strategies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Shizhe Yu, Haoren Wang, Lingpeng Yang, Yingxue Yan, Qiang Cai, Duo Ma, Long Jiang, Zehai Gao, Zhiyong Yu, Zongping Xia
Summary: The comprehensive study of the spatial-cellular anatomy of the human liver is crucial for understanding the cellular origins of liver disease. In this study, spatial transcriptomics were conducted on normal human liver tissue sections, providing detailed transcriptional information on liver zonation. A total of 6581 high-quality spots from normal livers were analyzed, mainly consisting of hepatocytes, which were classified into four sub-groups. These data serve as a reliable reference for studying the spatial heterogeneity of liver lobules.
Review
Immunology
Xiaoqian Liang, Caixin Ou, Jiayi Zhuang, Jinsheng Li, Fangfei Zhang, Yuanqiu Zhong, Yongfeng Chen
Summary: Psoriasis is a multifactorial immune-mediated disease with incompletely elucidated specific pathogenesis, and the skin microbiota may play a significant role in its provocation or exacerbation, interacting with innate immunity to stimulate T-cell populations and trigger immune cascade responses that ultimately lead to psoriasis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Plant Sciences
Ming Xin, Changbao Li, Hock Eng Khoo, Li Li, Xuemei He, Ping Yi, Yayuan Tang, Jian Sun
Summary: This study aimed to evaluate the changes in aromatic components of Tainong mango during fruit development, ripening, and storage, and identified the potential molecular insights of mango development and aroma formation in the fruit, revealing that volatile components and gene expression levels change with fruit ripening and storage time.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
T. Martynova, P. Kamanda, C. Sim
Summary: Identifying sex-specific gene expression patterns in Culex mosquitoes during pupal and adult stages using RNA-seq analysis provides important insights into the development and physiology of these disease vectors, which will aid in the development of more effective control methods.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Dermatology
Kim Klapan, Ziva Frangez, Nikita Markov, Shida Yousefi, Dagmar Simon, Hans-Uwe Simon
Summary: Atopic dermatitis and psoriasis are common chronic inflammatory skin diseases. Autophagy dysfunction may be associated with the pathogenesis of these diseases, in which the cytokine TNF-alpha may play a dual role in regulating autophagy.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Ophthalmology
Gaetan Le-Bel, Manel Benhassine, Solange Landreville, Sylvain L. Guerin
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Cell & Tissue Engineering
Pascale Desjardins, Camille Couture, Lucie Germain, Sylvain L. Guerin
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Gaetan Le-Bel, Sergio Cortez Ghio, Louis-Philippe Guerin, Francis Bisson, Lucie Germain, Sylvain L. Guerin
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Genetics & Heredity
Genevieve Rioux, Zainab Ridha, Melissa Simard, Florence Turgeon, Sylvain L. Guerin, Roxane Pouliot
Review
Biochemistry & Molecular Biology
Louis-Philippe Guerin, Gaetan Le-Bel, Pascale Desjardins, Camille Couture, Elodie Gillard, Elodie Boisselier, Richard Bazin, Lucie Germain, Sylvain L. Guerin
Summary: Every day, a large number of American workers require medical treatment for eye injuries sustained at work; corneal diseases rank as the fifth leading cause of blindness globally, and can be treated through corneal transplantation; tissue-engineered corneal substitutes show promise as future alternatives for corneal transplants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Melissa Simard, Genevieve Rioux, Sophie Morin, Cyril Martin, Sylvain L. Guerin, Nicolas Flamand, Pierre Julien, Julie Fradette, Roxane Pouliot
Summary: Clinical studies have shown that diets enriched with omega-3 polyunsaturated fatty acids could alleviate psoriasis symptoms, but the mechanisms are poorly understood. This study investigated the effects of ALA on psoriatic keratinocytes in a 3D skin model. ALA supplementation decreased keratinocyte proliferation, improved epidermal differentiation, and altered levels of lipid mediators.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Engineering, Biomedical
Genevieve Rioux, Melissa Simard, Sophie Morin, Isabelle Lorthois, Sylvain L. Guerin, Roxane Pouliot
Summary: This study developed an advanced in vitro 3D psoriatic skin model using activated T cells and cells from psoriatic skin, mimicking psoriatic characteristics and inflammatory environment. The model proved effective for investigating epidermal and T cell interaction.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Christelle Gross, Gaetan Le-Bel, Pascale Desjardins, Manel Benhassine, Lucie Germain, Sylvain L. Guerin
Summary: This study investigated the transcriptional pattern of corneal epithelial cells during deep wound healing in hTECs, revealing a significant repression of CLU gene expression during wound closure, which may be a prerequisite for hTEC wound closure. The study also identified regulatory regions in the CLU gene promoter and demonstrated that transcription factors AP-1 and Sp1/Sp3 play a role in the repression of CLU gene expression during corneal wound healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Manel Benhassine, Gaetan Le-Bel, Sylvain L. Guerin
Summary: This study identified the expression pattern of STAT family members in uveal melanoma and evaluated their contribution to HTR2B gene expression. The results showed that STAT proteins play a positive role in regulating the expression of HTR2B in UM cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Pascale Desjardins, Gaetan Le-Bel, Sergio C. Ghio, Lucie Germain, Sylvain L. Guerin
Summary: This study elucidates the molecular mechanisms by which WNK1 contributes to corneal wound healing. By regulating the activity and expression of transcription factors, WNK1 can impact the transcription of multiple genes, thereby controlling the migration, proliferation, and differentiation of corneal epithelial cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Clinical Neurology
Vincent Roy, Jay P. Ross, Remy Pepin, Sergio Cortez Ghio, Alyssa Brodeur, Lydia Touzel Deschenes, Gaetan Le-Bel, Daniel E. Phillips, Genevieve Milot, Patrick A. Dion, Sylvain Guerin, Lucie Germain, Francois Berthod, Francois A. Auger, Guy A. Rouleau, Nicolas Dupre, Francois Gros-Louis
Summary: This study reveals that RNF213 may play a crucial role in maintaining cerebral endothelial cell integrity, and its disruption may contribute to the early pathological mechanism of Moyamoya disease. Additionally, the study further emphasizes the importance of blood-brain barrier integrity.
Article
Biochemistry & Molecular Biology
Pascale Desjardins, Rebecca Berthiaume, Camille Couture, Gaetan Le-Bel, Vincent Roy, Francois Gros-Louis, Veronique J. Moulin, Stephanie Proulx, Sylvain Chemtob, Lucie Germain, Sylvain L. Guerin
Summary: Corneal wound healing involves communication between different cell types, which is partially mediated by exosomes. This study isolated exosomes released by different corneal cell types and found that they can accelerate wound healing. Additionally, exosomes alter the expression pattern of regulated genes. These findings are important for understanding the mechanisms of corneal wound repair and may lead to new therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Gaetan Le-Bel, Pascale Desjardins, Christelle Gross, Sergio Cortez Ghio, Camille Couture, Lucie Germain, Sylvain L. Guerin
Summary: This study evaluated the impact of postmortem/storage time on the properties of human limbal epithelial cells (hLECs) used for tissue-engineered human corneas (hTECs). The results showed that hLECs with a short postmortem/storage time had higher proliferative capacity and faster wound healing compared to those with a long postmortem/storage time, resulting in higher quality hTECs.
Article
Cell Biology
Genevieve Rioux, Florence Turgeon, Gaetan Le-Bel, Camille Grenier, Sylvain L. Guerin, Roxane Pouliot
Summary: Psoriasis is a complex, immune-mediated skin disease whose underlying mechanisms are largely unknown. In this study, a tissue-engineered human skin substitute enriched in T cells was used to investigate the cellular and molecular mechanisms involved in psoriasis. Gene profiling revealed significant changes in gene expression between psoriatic and healthy skin substitutes, and two deregulated genes, PTPRM and NELL2, were found to influence the ERK1/2 signaling pathway. Increased DNA-binding properties of NF-kappa B and Sp1 transcription factors, downstream targets of ERK1/2, were observed under psoriatic conditions. Inhibition of RSK, a downstream effector of ERK1/2, showed therapeutic potential for psoriasis treatment.
Article
Biochemistry & Molecular Biology
Sergio Cortez Ghio, Martin A. Barbier, Emilie J. Doucet, Imad Debbah, Meryem Safoine, Gaetan Le-Bel, Andreanne Cartier, Emilie Jolibois, Amelie Morissette, Danielle Larouche, Julie Fradette, Sylvain L. Guerin, Alain Garnier, Lucie Germain
Summary: We developed a new chemically defined serum-free medium, Surge SFM, which can support massive expansion of human primary keratinocytes and production of skin substitutes. Compared to conventional serum-containing medium, Surge SFM can more efficiently expand keratinocytes and properly form stratified epidermis. It also retains keratin-19-positive epithelial stem cells and maintains similar gene expression as with the conventional medium. Surge SFM can be used to produce bilayered self-assembled skin substitutes histologically similar to those produced using serum-containing medium, which can persist for up to six months when grafted onto mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
