期刊
PURINERGIC SIGNALLING
卷 13, 期 1, 页码 13-26出版社
SPRINGER
DOI: 10.1007/s11302-016-9537-0
关键词
Microvesicles; Microglia; Neuropathic pain; Spinal nerve ligation, P2X7
资金
- National Natural Science Foundation of China [81371253]
- Shanghai Municipal Education Commission [14ZZ083]
- Shanghai Changzheng Hospital [2015CZQN01]
Microglia are critical in the pathogenesis of neuropathic pain. In this study, we investigated the role of microvesicles (MVs) in neuropathic pain induced by spinal nerve ligation (SNL) in rats. First, we found that MVs shed from microglia were increased in the cerebrospinal fluid and dorsal horn of the spinal cord after SNL. Next, MVs significantly reduced paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). In addition, the P2X7-p38 pathway was related to the bleb of MVs after SNL. Interleukin (IL)-1 beta was found to be significantly upregulated in the package of MVs, and PWT and PWL increased following inhibition with shRNA-IL-1 beta. Finally, the amplitude and frequency of spontaneous excitatory postsynaptic currents increased following stimulation with MVs. Our results indicate that the P2X7-p38 pathway is closely correlated with the shedding of MVs from microglia in neuropathic pain, and MVs had a significant effect on neuropathic pain by participating in the interaction between microglia and neurons.
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