Article
Biochemistry & Molecular Biology
Pramod Shah, Chien-Sheng Chen
Summary: Antimicrobial peptides (AMPs) show promise as alternatives to combat various pathogenic microorganisms, with marine AMPs such as Polyphemusin-I targeting nucleic acid-related proteins. Studies have identified intracellular protein targets of Polyphemusin-I and revealed differences in protein targets between marine and terrestrial AMPs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pramod Shah, Chien-Sheng Chen
Summary: Sub5 is a synthetic AMP effective against fungi, with unknown targets, but identified to have 128 potential protein targets, primarily involving actin-associated proteins. Various enrichment analyses revealed significant associations with actin-related processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Infectious Diseases
Davor Juretic
Summary: This review focuses on peptides that can easily reach intracellular targets with little or no toxicity to mammalian cells, specifically optimized for cell-penetrating, antimicrobial, anticancer, antiviral, antifungal, and anti-inflammatory activity. The review suggests exploring wide-spectrum multifunctionality for novel nontoxic hybrids with cell-penetrating peptides.
Article
Biochemistry & Molecular Biology
Pramod Shah, Chien-Sheng Chen
Summary: Cell-penetrating peptides (CPPs) are peptides that can translocate across the cell envelope and have been used as vehicles for drug delivery. In this study, the potential protein targets of Penetratin, a well-known CPP, were screened using high-throughput proteome microarrays. The results showed that Penetratin targets proteins involved in ribonucleoprotein complex biogenesis, nucleic acid metabolic processes, and protein-protein interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
Qi-Yu Zhang, Zhi-Bin Yan, Yue-Ming Meng, Xiang-Yu Hong, Gang Shao, Jun-Jie Ma, Xu-Rui Cheng, Jun Liu, Jian Kang, Cai-Yun Fu
Summary: Antimicrobial peptides (AMPs) are a promising candidate to combat antibiotic resistant bacteria, showing broad-spectrum antimicrobial activity with low toxicity and utilizing sophisticated mechanisms of action. This comprehensive review provides an overview of the origin, structural characteristics, mechanisms of action, biological activities, and clinical applications of AMPs. Strategies to optimize and develop AMP-based treatments for potential antimicrobial and anticancer therapeutics are also discussed.
MILITARY MEDICAL RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Laura K. Buckton, Marw N. Rahimi, Shelli R. McAlpine
Summary: Developing macrocyclic peptides that can reach intracellular targets is a significant challenge, and current chemical strategies can improve cell permeability, but often at the expense of biological activity.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Medicinal
Rikeshwer Prasad Dewangan, Devesh Pratap Verma, Neeraj Kumar Verma, Ankit Gupta, Garima Pant, Kalyan Mitra, Saman Habib, Jimut Kanti Ghosh
Summary: In this study, a new type of proline-rich peptide mimetic was designed and synthesized. These lipopeptides exhibited significant antimicrobial activity against resistant bacteria and demonstrated resistance against trypsin digestion. They also showed synergistic antimicrobial activity when combined with tested antibiotics. The lipopeptides were found to interact with the bacterial ribosome and inhibit protein synthesis. Furthermore, they demonstrated superior clearance of bacteria compared to a conventional antibiotic.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Immunology
Diana Ivonne Duarte-Mata, Mario Cesar Salinas-Carmona
Summary: Intracellular bacteria cause difficult-to-resolve infections, and standard therapy antibiotics are often ineffective due to poor cellular uptake. Antimicrobial peptides (AMPs) show promise as a therapeutic approach because of their bactericidal properties and ability to modulate immune responses. This review focuses on AMPs used to treat intracellular bacterial infections and their impact on immune mechanisms.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Igor S. D. Costa, Tiago Junot, Fernanda L. Silva, Wanessa Felix, Jose L. Cardozo Fh, Antonio F. Pereira de Araujo, Constanca Pais do Amaral, Sonia Goncalves, Nuno C. Santos, Jose R. S. A. Leite, Carlos Bloch Jr, Guilherme D. Brand
Summary: The existence of antimicrobial encrypted fragments in human proteins has been demonstrated, and algorithms have been developed to identify these segments on a large scale. These fragments are typically cationic and amphiphilic segments that exert antimicrobial activity by disrupting membranes. The study found that about 2% of all human proteins contain these segments, with a higher prevalence in transmembrane and peripheral membrane proteins. These segments have conservation scores similar to other residues with similar solvent accessibility.
Article
Chemistry, Multidisciplinary
Atsushi Ohta, Mikimasa Tanada, Shojiro Shinohara, Yuya Morita, Kazuhiko Nakano, Yusuke Yamagishi, Ryusuke Takano, Shiori Kariyuki, Takeo Iida, Atsushi Matsuo, Kazuhisa Ozeki, Takashi Emura, Yuuji Sakurai, Koji Takano, Atsuko Higashida, Miki Kojima, Terushige Muraoka, Ryuuichi Takeyama, Tatsuya Kato, Kaori Kimura, Kotaro Ogawa, Kazuhiro Ohara, Shota Tanaka, Yasufumi Kikuchi, Nozomi Hisada, Ryuji Hayashi, Yoshikazu Nishimura, Kenichi Nomura, Tatsuhiko Tachibana, Machiko Irie, Hatsuo Kawada, Takuya Torizawa, Naoaki Murao, Tomoya Kotake, Masahiko Tanaka, Shiho Ishikawa, Taiji Miyake, Minoru Tamiya, Masako Arai, Aya Chiyoda, Sho Akai, Hitoshi Sase, Shino Kuramoto, Toshiya Ito, Takuya Shiraishi, Tetsuo Kojima, Hitoshi Iikura
Summary: Establishing a technological platform for creating clinical compounds that inhibit intracellular protein-protein interactions is crucial for developing valuable drugs. This study validated a new methodology for creating oral drugs that surpass the rule of 5 and can target intracellular tough proteins. The researchers also discovered a KRAS inhibitory clinical compound as an example of their platform technology.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Shang-Chuen Wu, Nourine A. Kamili, Marcelo Dias-Baruffi, Cassandra D. Josephson, Matthew F. Rathgeber, Melissa Y. Yeung, William J. Lane, Jianmei Wang, Hau-Ming Jan, Seth Rakoff-Nahoum, Richard D. Cummings, Sean R. Stowell, Connie M. Arthur
Summary: Adaptive immunity is regulated by tolerogenic mechanisms, but it faces challenges in combating microbes that mimic self-antigens. A study shows that the innate immune lectin Galectin-7 (Gal-7) can recognize various microbes that display blood group-like antigens, leading to their loss of viability without causing harm to host cells.
Review
Immunology
Danieli F. Buccini, Marlon H. Cardoso, Octavio L. Franco
Summary: Bacterial infections caused by intracellular pathogens are difficult to control with conventional antibiotic therapies, leading to increased interest in antimicrobial peptides and cell-penetrating peptides as potential alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Food Science & Technology
Muhammad Zohaib Aslam, Shumaila Firdos, Zhousi Li, Xiang Wang, Yangtai Liu, Xiaojie Qin, Shuo Yang, Yue Ma, Xuejuan Xia, Bolin Zhang, Qingli Dong
Summary: With the increase in antibiotic resistance, the focus has shifted to antimicrobial peptides (AMPs) as alternatives to antibiotics. AMPs have the ability to disrupt bacterial membranes and kill pathogens. This review discusses the structure and properties of AMPs, spectroscopy techniques used for evaluating their targeting mechanism and safety, and the production of AMPs from animal source, such as whey protein. It emphasizes the importance of utilizing whey protein to produce safe AMPs, which can enhance their efficacy and stability while reducing environmental pollution.
Article
Biochemistry & Molecular Biology
Beatriz Lima, Maria Ricci, Adriana Garro, Tunde Juhasz, Imola Csilla Szigyarto, Zita Papp, Gabriela Feresin, Jose Garcia de la Torre, Javier Lopez Cascales, Livia Fulop, Tamas Beke-Somfai, Ricardo D. Enriz
Summary: A theoretical and experimental study was conducted on a new series of small-sized antibacterial peptides, showing that these peptides have a unique mechanism of action and pharmacophoric requirements. Evaluation of physiochemical parameters modulating antimicrobial activity and biophysical characterization of the peptides revealed important insights for designing new antibacterial peptides with specific structural characteristics.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Food Science & Technology
Jose J. L. Silva, Maria R. A. de Miranda, Pedro F. N. Souza
Summary: Fungal contamination is a major cause of food spoilage, posing serious risks to human health and economic losses. This review discusses the recent advances in using synthetic peptides as agents to address foodborne fungal resistance and their applications in food preservation.
CURRENT OPINION IN FOOD SCIENCE
(2023)