期刊
PROTEOMICS
卷 16, 期 5, 页码 783-798出版社
WILEY
DOI: 10.1002/pmic.201500305
关键词
Community proteomics; Metaproteomics; Microbiology; Microbiota; Model systems
资金
- U.S. DOE-BER
- Innovation Fund Denmark (EcoDesign MBR)
- Innovation Fund Denmark (NomiGas)
- Danish Council for Independent Research
- Aalborg University, Denmark
- Carl-Zeiss-Stiftung
- DFG-Cluster of Excellence Inflammation at Interfaces, CL-X
Metaproteomicsthe large-scale characterization of the entire protein complement of environmental microbiota at a given point in timehas provided new features to study complex microbial communities in order to unravel these black boxes. New technical challenges arose that were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. Following a short introduction to microbial communities and metaproteomics, we introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters, and activated sludge. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability, or reliable protein extraction. The implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics.
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