Article
Biochemistry & Molecular Biology
Xiaoxue Cheng, Xiaosong Li, Yuexi Kang, Decai Zhang, Qiubo Yu, Junman Chen, Xinyu Li, Li Du, Tiantian Yang, Yao Gong, Ming Yi, Songzhi Zhang, Shasha Zhu, Shijia Ding, Wei Cheng
Summary: This study proposes a new strand displacement reaction model to enhance the efficiency of RNA in situ imaging. The approach allows for monitoring changes in gene expression within cells and holds potential value for clinical applications.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biophysics
Ssu-Chia Chen, Kuan-Ting Chen, Amily Fang-Ju Jou
Summary: The novel dual-amplification-boosted electrochemical biosensor was developed for detecting pancreatic cancer-associated miR-196b, showing a detection limit of 0.26 pM and nice analytical reproducibility. The biosensor offers great promise as a complementary tool for real sample detection in the clinic.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yanmei Zhou, Shupu Xie, Bo Liu, Cong Wang, Yibo Huang, Xiaoru Zhang, Shusheng Zhang
Summary: In this study, a chemiluminescence biosensor based on CRISPR-Cas12a and cation exchange reaction was developed for the detection of miRNA-21. The biosensor utilized rolling circle amplification and transcleavage activity of CRISPR-Cas12a to convert target RNA into DNA triggers, leading to the inhibition of CuS nanoparticles' binding to streptavidin and suppression of the cation exchange reaction, resulting in a signal off biosensor. The developed biosensor showed high sensitivity with a detection limit of 16 aM for miRNA-21 and can be used with both professional chemiluminescence instruments and smartphones for portable and low-cost assays.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Gerda Kildisiute, Waleed M. Kholosy, Matthew D. Young, Kenny Roberts, Rasa Elmentaite, Sander R. van Hooff, Clarissa N. Pacyna, Eleonora Khabirova, Alice Piapi, Christine Thevanesan, Eva Bugallo-Blanco, Christina Burke, Lira Mamanova, Kaylee M. Keller, Karin P. S. Langenberg-Ververgaert, Philip Lijnzaad, Thanasis Margaritis, Frank C. P. Holstege, Michelle L. Tas, Marc H. W. A. Wijnen, Max M. van Noesel, Ignacio Del Valle, Giuseppe Barone, Reinier van der Linden, Catriona Duncan, John Anderson, John C. Achermann, Muzlifah Haniffa, Sarah A. Teichmann, Dyanne Rampling, Neil J. Sebire, Xiaoling He, Ronald R. de Krijger, Roger A. Barker, Kerstin B. Meyer, Omer Bayraktar, Karin Straathof, Jan J. Molenaar, Sam Behjati
Summary: The neuroblastoma cancer cells resemble fetal sympathoblasts, indicating a pan-neuroblastoma cancer cell state that could potentially be targeted for novel treatment strategies. The findings highlight the importance of understanding the phenotype of neuroblastoma cells for the development of immunotherapeutic and targeted avenues.
Article
Chemistry, Multidisciplinary
Shanshan Yu, Jinhua Shang, Shizhen He, Qing Wang, Ruomeng Li, Yingying Chen, Xiaoqing Liu, Fuan Wang
Summary: In this study, a high-contrast in vivo imaging of microRNA (miRNA) was achieved by integrating an entropy-driven catalytic DNA amplification machine with on-site amplified substrate exposure procedure. The undesired signal leakage was eliminated by grafting substrate subunits into an auxiliary DNA polymerization amplification accessory. The intact machine-propelling functions were restored by cell-specific mRNA, ensuring the high specificity of on-site amplified miRNA imaging. This intelligent machinery shows great potential in biomedical diagnosis.
Article
Multidisciplinary Sciences
Yingfeng Tao, Xiaoliu Zhou, Leqiang Sun, Da Lin, Huaiyuan Cai, Xi Chen, Wei Zhou, Bing Yang, Zhe Hu, Jing Yu, Jing Zhang, Xiaoqing Yang, Fang Yang, Bang Shen, Wenbao Qi, Zhenfang Fu, Jinxia Dai, Gang Cao
Summary: In this study, the authors developed a versatile pi-FISH rainbow method that can efficiently detect a range of biomolecules, including DNA, RNA, proteins, and neurotransmitters. They successfully applied this method to detect gene expression in different species and decoded the spatial distribution of neuron subclusters using only two rounds of hybridization. Furthermore, they combined pi-FISH rainbow with hybridization chain reaction to develop pi-FISH+ technology for detecting short nucleic acid fragments in circulating tumor cells.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Lulu Guo, Huan Li, Rujian Zhao, Yidan Tang, Bingling Li
Summary: The novel RNA sensing platform combines RNA:DNA hybridization, DSN amplification, and PGM readout, showing sensitivity, specificity, and general applicability to different types of RNA. The simple and instrument-free detection procedure holds great potential for the development of portable and point-of-care measurements.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Tingting Xu, Yao Sun, Sha Yu, Shaojun Wu, Yu Su, Ye Tian, Yuanzhen Zhou, Jun-Jie Zhu
Summary: A novel fluorogenic RNA aptamer nanodevice has been developed, which integrates an entropy-driven RNA amplifier with near-infrared light control to achieve high contrast and sensitivity for imaging low-abundance mRNA in living cells. This design principle offers a new approach for developing low-background imaging systems for live-cell studies and manipulation.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Lars E. Borm, Alejandro Mossi Albiach, Camiel C. A. Mannens, Jokubas Janusauskas, Ceren Ozgun, David Fernandez-Garcia, Rebecca Hodge, Francisca Castillo, Charlotte R. H. Hedin, Eduardo J. Villablanca, Per Uhlen, Ed S. Lein, Simone Codeluppi, Sten Linnarsson
Summary: EEL FISH is a method that allows for rapid processing of large tissue samples while maintaining high resolution. By electrophoretically transferring RNA, EEL speeds up data acquisition and preserves single-cell resolution, enabling the study of spatial transcriptomes in the mouse brain and the human visual cortex.
NATURE BIOTECHNOLOGY
(2023)
Article
Oncology
Amelie Boichard, Scott M. Lippman, Razelle Kurzrock
Summary: The study found that high-level gene amplifications may not necessarily lead to overexpression at the RNA level, and non-focal amplifications are more likely to be silenced. If non-focal amplifications do result in overexpression, they may have therapeutic potential. Silencing specific high-level focal or non-focal gene amplifications could explain resistance to targeted drugs.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zhenming Zhang, Xin Ma, Jinwen Zhu, Ruhong Yan, Peng Miao
Summary: The study introduces a roll-to-roll DNA nanomachine for highly sensitive electrochemical detection of miRNA, offering a wide detection range with a limit of detection as low as 10 aM. The practicality is demonstrated by satisfactory results in human serum samples.
Article
Biochemical Research Methods
Chen Lin, Meng Jiang, Ling Liu, Xiaoyuan Chen, Yuancun Zhao, Lu Chen, Yujuan Hong, Xin Wang, Chengye Hong, Xihu Yao, Rongqin Ke
Summary: The asmFISH assay introduces a more efficient and specific method for highly specific imaging of individual transcripts in fixed cells and tissues, compared to the padlock probe assay. It can be applied not only to cultured cells, but also to fresh frozen and formalin-fixed, paraffin-embedded tissue sections.
Article
Biophysics
Jin Jiao, Chengjie Duan, Ji Zheng, Dayong Li, Chao Li, Zhongyun Wang, Tao Gao, Yang Xiang
Summary: The competitive endogenous RNA hypothesis involves circRNA-miRNA interaction in the regulation of gene expression, particularly in tumors and other diseases. A biosensing method has been developed for directly analyzing cmRRI, providing insights into RNA dialogue strategy.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Yinghui Feng, Qi Liu, Xinyi Zhao, Miao Chen, Xiaotong Sun, Hexiang Li, Xiaoqing Chen
Summary: In this study, a framework nucleic acid (FNA)-based nonenzymatic spatial-confinement amplifier was developed for rapid and reliable intracellular miRNA imaging. By encapsulating the localized catalytic hairpin assembly (L-CHA) reactor in the inner cavity of the FNA and utilizing the spatial-confinement effect, the reaction rate was accelerated and the dynamic performance was improved.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mengdie Li, Jiayang He, Xin Shang, Chunli Yang, Yuqing Zhang, Siyu Zuo, Ruo Yuan, Wenju Xu
Summary: In this study, a fluorescence platform based on rHCR was developed for signal amplification. By manipulating a specific DNA trigger and a T-analogous amplicon, multi green-emissive Ag nanoclusters can be concatenated for amplifiable signal readout. The rational combination of nonenzymatic rHCR and label-free fluorescent concatemers enables simplified, rapid, and highly sensitive detection.
ANALYTICAL CHEMISTRY
(2022)
