Article
Immunology
Chenyang Han, Yongjia Sheng, Jin Wang, Xiaohong Zhou, Wenyan Li, Caiqun Zhang, Li Guo, Yi Yang
Summary: By activating ROS, NOX4 promotes the M1 polarization of intestinal macrophages in inflammatory bowel disease (IBD), further exacerbating intestinal inflammation and mucosal barrier injury.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anthony L. Sylvester, David X. Zhang, Sophia Ran, Natalya S. Zinkevich
Summary: Reactive oxygen species (ROS) play important roles in health and disease, with both beneficial and harmful effects. Different sources of ROS have different roles in various pathologies. NOX1 is associated with hypertension through ROS production, making it a promising therapeutic target. NOX2 and NOX4 produce hydrogen peroxide, influencing cellular processes. Increased ROS production from NOX5 contributes to atherosclerosis.
Editorial Material
Plant Sciences
Pooja Singh, Vipul Mishra, Durgesh Kumar Tripathi, Francisco J. Corpas, Vijay Pratap Singh
Summary: Reactive oxygen species (ROS) play a crucial role in plant development and stress response. Recent research by Li et al. showed that ROS production is regulated by receptor-like cytoplasmic kinase (RLCK)-mediated phosphorylation of respiratory burst oxidase homolog D (RBOHD), which in turn modulates plant immunity and enhances biotic stress tolerance.
TRENDS IN PLANT SCIENCE
(2022)
Article
Immunology
Dipasree Hajra, Abhilash Vijay Nair, Atish Roy Chowdhury, Soumyadeep Mukherjee, Ritika Chatterjee, Dipshikha Chakravortty
Summary: This study reveals a novel role of U32 peptidase YdcP in Salmonella Typhimurium by protecting the bacteria from oxidative stress. The knockout of YdcP leads to attenuated intracellular proliferation of Salmonella and increased susceptibility to in vitro oxidative stress.
MICROBIAL PATHOGENESIS
(2022)
Article
Plant Sciences
Hirofumi Yoshioka, Yuta Hino, Keiichiro Iwata, Takaya Ogawa, Miki Yoshioka, Nobuaki Ishihama, Hiroaki Adachi
Summary: Plants exhibit sequential immune responses, pattern-triggered immunity (PTI), followed by effector-triggered immunity (ETI). Long-lasting MAPK activity during ETI induces robust ROS burst mediated by an NADPH oxidase in the plasma membrane, as well as in chloroplasts. Mining hydrogen peroxide receptors and ROS sensor proteins is crucial for understanding ROS-dependent cellular events. Additionally, a live-imaging system for MAPK activity at the single cell and organelle levels using a FRET-based biosensor is introduced. Spatiotemporal observations of MAPK activities in response to pathogen and herbivore attacks will offer new insights into the role of MAPK and ROS in plant immunity.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Min Jeong Kim, Su Jeong Ha, Bo Ram So, Chang-Kil Kim, Kyung-Min Kim, Sung Keun Jung
Summary: The skin, as the primary defense organ, is commonly affected by skin cancer due to cell degeneration caused by UV radiation. Nicotinamide adenine dinucleotide phosphate oxidase (NOX) and epidermal growth factor receptor (EGFR) play crucial roles in UV-induced skin carcinogenesis.
Article
Biochemistry & Molecular Biology
Lukas Gola, Laura Bierhansl, Julia Csatari, Christina B. Schroeter, Lisanne Korn, Venu Narayanan, Manuela Cerina, Sara Abdolahi, Anna Speicher, Alexander M. Hermann, Simone Koenig, Albena T. Dinkova-Kostova, Tawfeeq Shekh-Ahmad, Sven G. Meuth, Heinz Wiendl, Ali Gorji, Matthias Pawlowski, Stjepana Kovac
Summary: Hyperexcitability is associated with neuronal dysfunction, cellular death, and neurodegeneration. NOX4, an NADPH oxidase, is found to regulate reactive oxygen species (ROS) levels and calcium homeostasis, thereby preventing hyperexcitability and neuronal death. These findings suggest that NOX4 might have a crucial role in neurodegenerative diseases by acting as a potential redox regulator.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mara Fiorani, Rita De Matteis, Barbara Canonico, Giulia Blandino, Alessandro Mazzoli, Mariele Montanari, Andrea Guidarelli, Orazio Cantoni
Summary: The conversion of human SW872 preadipocytes to mature adipocytes involves time-dependent changes in differentiation markers' expression, morphological changes, and accumulation of lipid droplets. Reactive oxygen species (ROS) formation was significant at specific time points during differentiation, with NADPH oxidase (NOX)-2 playing a role. Mitochondrial ROS (mROS) were only detected in the late phase of differentiation, indicating potential mitochondrial dysfunction. These findings suggest a potential link between ROS, adipogenesis, and adipose tissue dysfunction in SW872 cells.
Article
Biochemistry & Molecular Biology
Stephenson B. Owusu, Elodie Hudik, Celine Ferard, Sophie Dupre-Crochet, Eric C. D. K. Addison, Kwasi Preko, Tania Bizouarn, Chantal Houee-Levin, Laura Baciou
Summary: Simulating oxidative stress with gamma irradiation leads to shortened half-lives of neutrophils but pre-activates surviving cells to produce superoxide anions. Incomplete assembly of the NADPH oxidase complex after irradiation significantly enhances neutrophil reactivity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Man-Hau Ho, Chia-Hung Yen, Tsung-Hsun Hsieh, Tzu-Jen Kao, Jing-Yuan Chiu, Yung-Hsiao Chiang, Barry J. Hoffer, Wen-Chang Chang, Szu-Yi Chou
Summary: This study found that CCL5 plays an important role in activating GPX1 antioxidant activity during days 1-3 post-injury, protecting hippocampal neurons from ROS damage, and improving memory function after trauma.
Article
Biochemistry & Molecular Biology
Rana M. Nassif, Elias Chalhoub, Pia Chedid, Margarita Hurtado-Nedelec, Elia Raya, Pham My-Chan Dang, Jean-Claude Marie, Jamel El-Benna
Summary: Metformin exhibits anti-inflammatory effects by inhibiting the differentiation of human monocytes into proinflammatory macrophages and by limiting ROS production by macrophages via the activation of AMPK.
Article
Microbiology
Hasnaa Maksouri, Dounia Darif, Jerome Estaquier, Myriam Riyad, Christophe Desterke, Meryem Lemrani, Pham My-Chan Dang, Khadija Akarid
Summary: This study evaluated the impact of Moroccan strains of L. major and L. tropica on PMNs. The results showed that L. tropica inhibited O-2(-) production, while L. major did not. Additionally, Leishmania soluble antigens from both species inhibited O-2(-) induction.
Article
Biochemistry & Molecular Biology
Ping Li, Lulu Zhao, Fan Qi, Nang Myint Phyu Sin Htwe, Qiuying Li, Dawei Zhang, Fucheng Lin, Keke Shang-Guan, Yan Liang
Summary: The study revealed that RIPK plays a crucial role in regulating ROS production in various layers of the plant immune system, contributing to immune responses. RIPK mutants showed reduced ROS production in response to immune elicitors and increased susceptibility to necrotrophic bacteria.
Article
Agronomy
Jie Li, Lidan Feng, Dong Li, Xianglin Liu, Yangyang Pan, Jing He, Junxia Zhang
Summary: This study investigates the role of ROS mediated by NADPH oxidase in the growth of F. solani. The results demonstrate that NCF2 gene plays a significant role in regulating the activity of NADPH oxidase and the expression of ROS metabolism enzyme genes, which affects colony growth in F. solani. Furthermore, exogenous H2O2 treatment also influences the growth and ROS metabolism of F. solani.
Article
Biotechnology & Applied Microbiology
Mengxue Liu, Yao Liu, Lei Zhang, Facheng Qiu
Summary: In this study, the function of NADPH oxidase in Chlorella pyrenoidosa was investigated. The results showed that NADPH oxidase actively participated in the production of intracellular ROS, and the ROS levels were higher under nitrogen and phosphorus-deficient conditions.
BIOTECHNOLOGY LETTERS
(2023)
Review
Pediatrics
Francesco Morini, Kelly M. A. Dreuning, Maarten J. H. Janssen Lok, Tomas Wester, Joep P. M. Derikx, Florian Friedmacher, Hiromu Miyake, Haitao Zhu, Luca Pio, Martin Lacher, Stefania Sgro, Augusto Zani, Simon Eaton, L. W. Ernest van Heurn, Agostino Pierro
Summary: This study evaluated the evidence on pediatric inguinal hernia repair and compiled recommendations on six topics including surgical approach, timing, and anesthesia technique. Results suggest that laparoscopic repair may be beneficial for children with bilateral hernia, while preterm infants may benefit from regional anesthesia and delayed surgery. Evidence for other topics was less conclusive.
EUROPEAN JOURNAL OF PEDIATRIC SURGERY
(2022)
Article
Surgery
Henning C. Fiegel, Stefan Gfroerer, Till-Martin Theilen, Florian Friedmacher, Udo Rolle
Summary: Ovarian lesions in children are mainly cysts in younger age groups, while teratomas or other germ cell tumors are more common in older children. Surgery should aim to preserve healthy ovarian tissue in all cases.
INNOVATIVE SURGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Govardhana R. Pinnelli, Erika Plettner
Summary: Fluorescent analogues of gypsy moth sex pheromone disparlure were synthesized and characterized for binding to pheromone-binding proteins LdisPBP1 and LdisPBP2. The fluorescence binding assay showed different affinities of disparlure enantiomers to the two proteins. Competitive binding assays demonstrated effective displacement of fluorescent disparlure by disparlure enantiomers.
JOURNAL OF CHEMICAL ECOLOGY
(2022)
Article
Pediatrics
Kerstin Saalabian, Florian Friedmacher, Till-Martin Theilen, Daniel Keese, Udo Rolle, Stefan Gfroerer
Summary: Background: Duodenal obstruction is a rare cause of congenital bowel obstruction. Prenatal ultrasound could be suggestive of duodenal atresia if polyhydramnios and the double bubble sign are visible. Prenatal diagnosis is important for prompt surgical treatment.
Article
Multidisciplinary Sciences
Liang-Cui Chu, Pedro Arede, Wei Li, Erika C. Urdaneta, Ivayla Ivanova, Stuart W. McKellar, Jimi C. Wills, Theresa Frohlich, Alexander von Kriegsheim, Benedikt M. Beckmann, Sander Granneman
Summary: RNA-binding proteins play a crucial role in controlling gene expression. This study identifies hundreds of RNA-binding proteins in methicillin-resistant Staphylococcus aureus (MRSA) and demonstrates that a major transcription factor binds RNAs near intrinsic transcription terminators using its helix-turn-helix domain.
NATURE COMMUNICATIONS
(2022)
Review
Pediatrics
Florian Friedmacher, Udo Rolle, Prem Puri
Summary: This article provides an up-to-date overview of congenital diaphragmatic hernia (CDH), including its implicated transcription factors, molecules regulating cell migration, and components contributing to extracellular matrix formation. The article also discusses the significance of genetic models in studying altered lung development in relation to the human situation.
FRONTIERS IN PEDIATRICS
(2022)
Article
Biology
Lewis Macdonald, Gillian C. Taylor, Jennifer Margaret Brisbane, Ersi Christodoulou, Lucy Scott, Alex von Kriegsheim, Janet Rossant, Bin Gu, Andrew J. Wood
Summary: Researchers have developed a genetic engineering method using CRISPR technology to efficiently degrade endogenous proteins in vivo, and found that degradation kinetics depend on the dosage of the protein, ligand, and substrate receptor. Applying this method to the study of key regulators of cell division, they discovered that these regulators are essential for cell division in precursor lymphocytes.
Article
Genetics & Heredity
Jennifer Lennon, Petra zur Lage, Alex von Kriegsheim, Andrew P. Jarman
Summary: Axonemal dynein motors are complex protein complexes that drive ciliary movement. The Drosophila homologues of DNAAF4 and DNAAF6, CG14921/Dnaaf4 and CG5048/Dnaaf6, are found to associate in a complex similar to R2TP and play a crucial role in dynein assembly during the development of motile cilia.
FRONTIERS IN GENETICS
(2022)
Review
Pediatrics
Till-Martin Theilen, Yannick Braun, Konrad Bochennek, Udo Rolle, Henning C. Fiegel, Florian Friedmacher
Summary: Significant progress has been made in the management of Wilms tumor in recent years, including collaborative efforts, protocol-driven therapy, and technical innovations. This article provides a comprehensive overview of current treatment strategies, highlighting the use of nephron-sparing surgery and minimally invasive approaches. It also discusses surgical concepts for metastatic disease, advances in tumor imaging technology, and potential prognostic biomarkers.
FRONTIERS IN PEDIATRICS
(2022)
Article
Multidisciplinary Sciences
Ana Martin-Vega, Laura Ruiz-Peinado, Rocio Garcia-Gomez, Ana Herrero, Dalia de la Fuente-Vivas, Swetha Parvathaneni, Ruben Caloto, Marta Morante, Alex von Kriegsheim, Xose R. Bustelo, David B. Sacks, Berta Casar, Piero Crespo
Summary: RAS-ERK pathway signals are regulated by scaffold proteins, and it is revealed that scaffold proteins can interact with each other and undergo phosphorylation reactions. The trans-phosphorylation process participates in KSR1-regulated adipogenesis and the cytotoxicity exhibited by KSR-directed inhibitors. This finding has implications in signaling and the design of scaffold protein-aimed therapeutics.
Article
Pharmacology & Pharmacy
Gabriela Dorcioman, Valentina Grumezescu, George E. Stan, Mariana Carmen Chifiriuc, Gratiela Pircalabioru Gradisteanu, Florin Miculescu, Elena Matei, Gianina Popescu-Pelin, Irina Zgura, Valentin Craciun, Faik Nuzhet Oktar, Liviu Duta
Summary: Novel biomaterials with excellent bone regeneration potential were synthesized from marine-derived hydroxyapatite by pulsed laser deposition. The rough surfaces of the thin films promoted cell adhesion and implant anchorage. The thin films exhibited strong hydrophilic behavior, superior bonding strength adherence, low cytotoxicity, and effective antimicrobial activity. These cost-effective materials from sustainable sources are recommended for the development of innovative coatings for metallic dental implants.
Article
Polymer Science
Maria Minodora Marin, Ioana Catalina Gifu, Gratiela Gradisteanu Pircalabioru, Madalina Albu Kaya, Rodica Roxana Constantinescu, Rebeca Leu Alexa, Bogdan Trica, Elvira Alexandrescu, Cristina Lavinia Nistor, Cristian Petcu, Raluca Ianchis
Summary: Natural polysaccharides are recommended for medical applications due to their low cytotoxicity and hydrophilicity. Polysaccharides can be used in additive manufacturing to achieve various customized 3D structures. In this study, silica nanoparticles were added to a microbial polysaccharide to obtain printable hydrogel nanocomposites.
Article
Oncology
Aoife Nolan, Cinzia Raso, Walter Kolch, Alex von Kriegsheim, Kieran Wynne, David Matallanas
Summary: RAS proteins play a crucial role in cell signalling, regulating cell functions such as proliferation, differentiation, and death. Mutations in genes of this family, particularly KRAS, are common and were believed to constitutively activate KRAS. However, recent findings show that some mutants can switch between active and inactive states. This, along with the development of covalent KRASG12C inhibitors, has led to the emergence of KRAS inhibitors in clinical use. Despite this, resistance to targeted therapies remains a challenge, and effective treatments for other KRAS mutants are lacking. To overcome these hurdles and accelerate RAS targeting therapies, a comprehensive understanding of the molecular mechanisms underlying KRAS signalling networks and the differences in downstream signalling of KRAS mutants is needed. This study employed affinity purification mass-spectrometry proteomics to analyze the interactome of KRAS wild-type and three KRAS mutants. Through bioinformatic analysis and experimental validation, the researchers mapped the signalling network mediated by different KRAS proteins. The study also revealed novel crosstalk between KRAS and effector pathways, including AKT and JAK-STAT signalling modules.
Review
Pediatrics
Florian Friedmacher, Udo Rolle
Summary: Interstitial cells of Cajal (ICCs) are pacemaker cells in the gastrointestinal tract that control motility by generating slow waves and inducing contractions. Traditionally, ICCs have been identified using the c-kit marker, but anoctamin-1 is now recognized as a more specific marker. This article provides an overview of ICCs' origin, distribution, and functions, and discusses various pediatric disorders involving ICC dysfunction.
PEDIATRIC SURGERY INTERNATIONAL
(2023)
Article
Pediatrics
Florian Friedmacher
Summary: The operative management of long-gap esophageal atresia (LGEA) is challenging due to its rarity and complexity. Efforts should be made to preserve the native esophagus, and the preferred treatment option is the creation of an initial gastrostomy followed by delayed primary anastomosis. This article provides an update on the technique, challenges, and long-term outcomes of this approach.
PEDIATRIC SURGERY INTERNATIONAL
(2023)