期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 113, 期 47, 页码 13390-13395出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1613825113
关键词
DNA replication; CMG; Archaea; MCM; helicase
资金
- Biotechnology and Biological Sciences Research Council
- Wellcome Trust
- Max Planck Society
- Indiana University College of Arts and Sciences
- BBSRC [BB/G00028X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G00028X/1] Funding Source: researchfish
The regulated recruitment of Cdc45 and GINS is key to activating the eukaryotic MCM(2-7) replicative helicase. We demonstrate that the homohexameric archaeal MCM helicase associates with orthologs of GINS and Cdc45 in vivo and in vitro. Association of these factors with MCM robustly stimulates the MCM helicase activity. In contrast to the situation in eukaryotes, archaeal Cdc45 and GINS form an extremely stable complex before binding MCM. Further, the archaeal GINS center dot Cdc45 complex contains two copies of Cdc45. Our analyses give insight into the function and evolution of the conserved core of the archaeal/eukaryotic replisome.
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