Article
Biology
Hongki Song, William T. Wickner, Adam Linstedt
Summary: The yeast vacuolar membrane fusion process can be achieved using a synthetic tether instead of the HOPS complex. This suggests that Sec17 and Sec18 play a crucial role in membrane fusion and may have a general role in promoting fusion across different cellular processes.
Article
Chemistry, Multidisciplinary
Mette Galsgaard Malle, Philipp M. G. Loeffler, Soren S-R Bohr, Magnus Berg Sletfjerding, Nikolaj Alexander Risgaard, Simon Bo Jensen, Min Zhang, Per Hedegard, Stefan Vogel, Nikos S. Hatzakis
Summary: In this study, a single-particle combinatorial multiplexed liposome fusion mediated by DNA is presented, enabling multistep and non-deterministic fusion of nanocontainers. Efficient and leakage-free stochastic fusion sequences were observed for arrays of target liposomes functionalized with individual DNA strands and labeled with distinct chromophores. The method allows high-throughput combinatorial multiplex screens using minimal material.
Article
Multidisciplinary Sciences
Rohith K. Nellikka, Bhavya R. Bhaskar, Kinjal Sanghrajka, Swapnali S. Patil, Debasis Das
Summary: Alpha-synuclein plays a crucial role in the pathogenesis of Parkinson's disease by directly regulating individual exocytotic release events, controlling vesicular secretion. It forms an inhibitory complex to decrease the open probability of fusion pores, influenced by various factors. This study reveals the key role of alpha-synuclein in modulating pore properties and highlights its regulatory action in membrane fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Paul Heo, Jeff Coleman, Jean-Baptiste Fleury, James E. Rothman, Frederic Pincet
Summary: Fusion pores of discrete sizes were observed during vesicle fusion with a lipid bilayer, with single SNAREpin and dual SNAREpin actions corresponding to different peaks in pore size distribution. The study provides insights into the energy landscape of fusion pores and the role of SNAREpins in cellular trafficking and neurotransmitter release mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Plant Sciences
Changxin Luo, Yumei Shi, Yun Xiang
Summary: SNARE proteins play essential roles in maintaining cellular homeostasis and regulating root growth and development in Arabidopsis. Recent studies have shown that SNAREs are involved in various fundamental processes and signaling networks related to vesicle trafficking.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Engineering, Environmental
Maura J. Donohue, Maily Pham, Stephanie Brown, Kaveri M. Easwaran, Stephen Vesper, Jatin H. Mistry
Summary: This study investigated the detection methods of Legionella pneumophila in tap water samples in buildings across the United States and their performance under different water quality conditions. The results showed variations in method performance when using three culture methods and one molecular method. Water quality influenced the detection results of L. pneumophila, especially the total organic carbon and heterotrophic bacterial counts were positively correlated with its detection frequency. Therefore, water quality should be considered when selecting L. pneumophila detection methods.
Review
Neurosciences
Chong Yan, Jie Jiang, Yuan Yang, Xiaoqi Geng, Wei Dong
Summary: This review highlights the roles of VAMP2 protein in the presynaptic cytoplasm, as well as its interaction with other fusion-related proteins. VAMP2 plays a crucial role in mediating synaptic vesicle fusion and neurotransmitter release during the fusion process.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Infectious Diseases
Jamie Gorzynski, Bryan Wee, Melissa Llano, Joana Alves, Ross Cameron, Jim McMenamin, Andrew Smith, Diane Lindsay, J. Ross Fitzgerald
Summary: Through whole-genome sequencing, this study investigates the evolutionary and epidemiological relationships of Legionella pneumophila isolates in Scotland over 36 years. The analysis reveals the presence of endemic clones in hospital, community, and travel-associated environments, and suggests the importance of redefining cluster and outbreak definitions. The study highlights the significance of whole-genome sequencing in real-time identification and mitigation of clinically important endemic clones.
Article
Biology
Pedro Escoll, Lucien Platon, Mariatou Drame, Tobias Sahr, Silke Schmidt, Christophe Rusniok, Carmen Buchrieser
Summary: Research shows that Legionella pneumophila reverses the activity of the mitochondrial FOF1-ATPase during infection, maintaining mitochondrial membrane potential and preventing macrophage cell death. This manipulation delays host cell death and preserves the bacterial replication niche, allowing for preferential bacterial replication in cells with maintained mitochondrial membrane potential.
Review
Biochemistry & Molecular Biology
Cilem Ozdemir, Nilfer Sahin, Tuba Edgunlu
Summary: Research has found that expression differences, abnormal expressions, or dysfunctions of SNARE complexes involved in neurodevelopment are associated with autism spectrum disorders. Further understanding of SNARE mechanisms is crucial for understanding and treating autism spectrum disorders.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Multidisciplinary Sciences
Tao-Tao Chen, Yanling Lin, Shijun Zhang, Shuxin Liu, Lei Song, Wenhong Zhong, Zhao-Qing Luo, Aidong Han
Summary: The intracellular bacterial pathogen Legionella pneumophila hijacks host vesicular transport factor p115 to localize its effector protein LegA15 in host lipid droplets (LDs) and causes fragmentation of host Golgi apparatus. LegA15 is an atypical GTPase that modulates host LD homeostasis through its GTPase activity.
Review
Microbiology
Ayesha Ayesha, Franklin Wang-Ngai Chow, Polly Hang-Mei Leung
Summary: Legionella pneumophila, an intracellular pathogen, utilizes OMVs to communicate with and modulate host immune response, playing a crucial role in disease pathogenesis. Understanding the functions of OMVs provides valuable insights for combating Legionnaires' disease.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Nathan K. Glueck, Kevin M. O'Brien, Danielle C. Seguin, Vincent J. Starai
Summary: Legionella pneumophila is a facultative intracellular bacterial pathogen that alters host organelle trafficking to construct the bacteria-laden LCV. The effector protein LegC7 functions to recruit host ER material to the bacterial phagosome during infection, potentially through interaction with host membrane tethering complexes and/or cargo adapters, inducing abnormal ER:endosome interactions.
Review
Medicine, General & Internal
Valeria Iliadi, Jeni Staykova, Sergios Iliadis, Ina Konstantinidou, Polina Sivykh, Gioulia Romanidou, Daniil F. Vardikov, Dimitrios Cassimos, Theocharis G. Konstantinidis
Summary: Legionella spp. is a potentially fatal bacterium that can cause pneumonia and can be transmitted through inhalation of aerosols. It can invade various organs and cause severe manifestations, including septic shock.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Marietta S. Kaspers, Vivian Pogenberg, Christian Pett, Stefan Ernst, Felix Ecker, Philipp Ochtrop, Michael Groll, Christian Hedberg, Aymelt Itzen
Summary: In this study, the authors uncover the mechanism and substrate recognition of Legionella effector Lem3, which removes a phosphocholine moiety from the human protein Rab1 during infection. They present the crystal structure of the stabilised Lem3:Rab1b complex, revealing the catalytic mechanism and substrate recognition of PPM phosphatases shaped Lem3. This research is of significant importance for understanding bacterial pathogenesis and protein substrate recognition.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Ilona Kondratiuk, Shrutee Jakhanwal, Jialin Jin, Udhayabhaskar Sathyanarayanan, Benjamin Kroppen, Ajaybabu Pobbati, Anita Krisko, Uri Ashery, Michael Meinecke, Reinhard Jahn, Dirk Fasshauer, Ira Milosevic
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Yelda Birinci, Julia Preobraschenski, Marcelo Ganzella, Reinhard Jahn, Yongsoo Park
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Nicole Scott-Hewitt, Fabio Perrucci, Raffaella Morini, Marco Erreni, Matthew Mahoney, Agata Witkowska, Alanna Carey, Elisa Faggiani, Lisa Theresia Schuetz, Sydney Mason, Matteo Tamborini, Matteo Bizzotto, Lorena Passoni, Fabia Filipello, Reinhard Jahn, Beth Stevens, Michela Matteoli
Article
Biophysics
Agata Witkowska, Susann Spindler, Reza Gholami Mahmoodabadi, Vahid Sandoghdar, Reinhard Jahn
BIOPHYSICAL JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Xiandeng Wu, Marcelo Ganzella, Jinchuan Zhou, Shihan Zhu, Reinhard Jahn, Mingjie Zhang
Summary: The tethering of synaptic vesicles to the active zone is determined by phase separation, forming two distinct SUV pools, which have general implications in cellular processes like vesicular formation and trafficking.
Article
Multidisciplinary Sciences
Zacharie Taoufiq, Momchil Ninov, Alejandro Villar-Briones, Han-Ying Wang, Toshio Sasaki, Michael C. Roy, Francois Beauchain, Yasunori Mori, Tomofumi Yoshida, Shigeo Takamori, Reinhard Jahn, Tomoyuki Takahashi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Cell Biology
Cyril Cheret, Marcelo Ganzella, Julia Preobraschenski, Reinhard Jahn, Gudrun Ahnert-Hilger
Summary: VGLUTs play a dual role in neurons by filling synaptic vesicles with glutamate and regulating intracellular Pi levels through transport activity. Studies have shown that VGLUT-mediated Pi influx is counteracted by Pi efflux, highlighting the importance of VGLUTs in both glutamate loading and Pi restoration.
Article
Biochemical Research Methods
Ivan Silbern, Kuan-Ting Pan, Maksims Fiosins, Stefan Bonn, Silvio O. Rizzoli, Eugenio F. Fornasiero, Henning Urlaub, Reinhard Jahn
Summary: The study investigates the impact of synaptic vesicle cycling-dependent phosphorylation events on various functions in synaptic transmission, including exo- and endocytosis processes.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Sabine Wittig, Marcelo Ganzella, Marie Barth, Susann Kostmann, Dietmar Riedel, Angel Perez-Lara, Reinhard Jahn, Carla Schmidt
Summary: The protein interactions within synaptic vesicles are complex and can be categorized into specific modules or interactions caused by 'crowding'. Understanding these interactions provides insights into the composition and function of synaptic vesicles.
NATURE COMMUNICATIONS
(2021)
Review
Biophysics
Tobias Grothe, Julia Nowak, Reinhard Jahn, Peter Jomo Walla
Summary: The passage describes various fluorescence-based methods developed over the past decade to monitor membrane fusion, docking, distances, and curvature. These tools were primarily developed using liposomes as model systems, and their application range and limitations are discussed with examples from protein-mediated vesicle docking and fusion studies.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Judyta K. Juranek, Konark Mukherjee, Reinhard Jahn, Jia-Yi Li
Summary: Synaptic transmission relies on neurotransmitters stored in synaptic vesicles and released at the active zone. Research shows a robust bi-directional, coordinated trafficking of synaptic vesicle and active zone proteins in peripheral nerves, which plays a critical role in neuronal function and neurodegenerative diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Agata Witkowska, Leonard P. Heinz, Helmut Grubmueller, Reinhard Jahn
Summary: Proteins driving membrane fusion must overcome energy barriers to induce intermediate steps where lipid bilayers' integrity is lost. The metastable intermediate preceding hemifusion does not require but is enhanced by divalent cations, characterized by the absence of proteins and local membrane thickening, induced by lipid rearrangements due to dehydration of the membrane surface revealed by simulations.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Changwon Kim, Min Ju Shon, Sung Hyun Kim, Gee Sung Eun, Je-Kyung Ryu, Changbong Hyeon, Reinhard Jahn, Tae-Young Yoon
Summary: Using single-molecule methods, researchers demonstrated the cooperativity between NSF and the SNARE complex to prevent ATP consumption without productive disassembly.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Seiichi Koike, Reinhard Jahn
Summary: Membrane traffic in eukaryotic cells is regulated by transport vesicles that contain targeting signals recognized by tethering factors. The final step, membrane fusion, is mediated by SNARE proteins. The role of SNARE proteins in targeting specificity is still a topic of debate.
BIOCHEMICAL JOURNAL
(2022)
Review
Cell Biology
Reinhard Jahn, David C. Cafiso, Lukas K. Tamm
Summary: This article reviews the SNARE-mediated fusion pathway, including the assembly of SNARE proteins into complexes, the regulation of this assembly by accessory proteins, and the overcoming of energy barriers that prevent membrane fusion.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)