Article
Multidisciplinary Sciences
Lei Zhang, James E. Kent, Meredith Whitaker, David C. Young, Dominik Herrmann, Alexander E. Aleshin, Ying-Hui Ko, Gino Cingolani, Jamil S. Saad, D. Branch Moody, Francesca M. Marassi, Sabine Ehrt, Michael Niederweis
Summary: The gene rv0455c is essential for the growth of Mycobacterium tuberculosis in low-iron conditions. Its absence results in reduced secretion of siderophores and impaired replication of the pathogen in mice. The crystal structure of Rv0455c provides insight into the unique siderophore secretion system of M. tuberculosis.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Jeong In Hur, Jinshil Kim, Sangryeol Ryu, Byeonghwa Jeon
Summary: This study demonstrates the existence of phylogenetic clusters in Campylobacter jejuni associated with cold stress tolerance and identifies the genetic element cfrA as a contributor to cold stress tolerance.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Qingli Song, Lishan Zhang, Guibin Wang, Huanhuan Song, Shuangziying Zhang, Jindong Yao, Xiangmin Lin
Summary: This study used quantitative proteomics to compare differentially expressed proteins between the delta ahcirA and wild-type strains of Aeromonas hydrophila. Bioinformatics analysis showed that the expression of peptide, histidine, and arginine ATP-binding cassette (ABC) transporter system-related proteins was significantly higher in the delta ahcirA strain. Further experiments revealed that delta ahcirA grew slower than the wild-type strain in nutrient-limited medium supplemented with dipeptide, histidine, and arginine. Far-western blot analysis confirmed that AhCirA can directly bind to histidine/arginine and dipeptide small-molecule substrates. These findings suggest that AhCirA plays a role in amino acid and peptide uptake as a channel-forming porin and interacts with ABC transporters to transport nutrient substances.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Immunology
Huiwen Wang, Ximin Zeng, Liu Cao, Qiang He, Jun Lin
Summary: The Ent conjugate vaccine effectively controlled Gram-negative pathogens, particularly the leading foodborne bacterial pathogen.
Article
Chemistry, Inorganic & Nuclear
Jinyoung Oh, Dahyun Kang, Sugyeong Hong, Sun H. Kim, Jun-Ho Choi, Jiwon Seo
Summary: Siderophore-mimicking macrocyclic peptoids were synthesized and studied for their stable catecholate-iron complex structure. Peptoid 3 showed comparable iron binding affinity to deferoxamine, with enhanced stability upon air exposure, as determined by UV-vis, fluorescence, EPR spectroscopies, and DFT calculations.
DALTON TRANSACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Anna M. Banas, Katarzyna M. Bocian-Ostrzycka, Stanislaw Dunin-Horkawicz, Jan Ludwiczak, Piotr Wilk, Marta Orlikowska, Agnieszka Wyszynska, Maria Dabrowska, Maciej Plichta, Marta Spodzieja, Marta A. Polanska, Agata Malinowska, Elzbieta Katarzyna Jagusztyn-Krynicka
Summary: The bacterial Dsb proteins catalyze the formation of disulfide bridges between cysteine residues, stabilizing protein structures. Campylobacter jejuni's oxidizing Dsb system consists of two monomeric DsbAs and one dimeric bifunctional protein. The two monomeric DsbAs in C. jejuni are dispensable proteins, with homologous structures to EcDsbL. Comparative proteomics showed that the Dsb system targets several respiratory and periplasmic transport proteins, essential for the bacterium's respiratory process in oxygen-limiting conditions. The detailed analysis provides insights into potential antibacterial targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, David Perez-Pascual, Rebecca J. Stevick, Clayton Birkett, Jean-Marc Ghigo, Mark J. McBride
Summary: Flavobacterium columnare, a costly pathogen in the freshwater fish-farming industry, produces siderophores for iron uptake. This study identifies genes involved in iron uptake and reveals their importance for growth under iron-limited conditions. Mutants with deletions in these genes showed decreased virulence but still caused infection. Survivors of exposure to these mutants were partially protected against later infection by the wild-type pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Parker R. Stow, Zachary L. Reitz, Timothy C. Johnstone, Alison Butler
Summary: Chirality plays a crucial role in the iron uptake process, influencing the configuration of Fe(iii) complexes. Synthesizing different diastereomers of ferric complexes of triscatechol siderophores helped define the impact of Lys and Ser residues on the configuration of the complexes. Genomic searches in microbes identified biosynthetic gene clusters responsible for producing these diastereomers, with relevant genes found in plant and opportunistic pathogens.
Article
Chemistry, Medicinal
Katsushiro Miyamoto, Hiroaki Kawano, Naoko Okai, Takeshi Hiromoto, Nao Miyano, Koji Tomoo, Takahiro Tsuchiya, Jun Komano, Tomotaka Tanabe, Tatsuya Funahashi, Hiroshi Tsujibo
Summary: Vibrio vulnificus M2799 secretes the catecholate siderophore vulnibactin to capture ferric ions for growth, utilizing a ferric-import system composed of various proteins including an isochorismate synthase and an outer membrane receptor. In infectious diseases, periplasmic-binding proteins and ferric-chelate reductases involved in the ferric-siderophore utilization system could be potential targets for drug discovery.
Article
Engineering, Environmental
Donglei Zhang, Xiaolei Liu, Yuefei Ding, Juan Liu, Hongchen Jiang, Hailiang Dong
Summary: The air oxidation rate of Cr1-x Fe x (OH)(3) in dark and alkaline environments is influenced by pH, the Fe/Cr ratio, and the presence of siderophores. The role of oxygen in the dark and the effects of organic ligands on Cr(III) oxidation are often overlooked. This study investigates the kinetics of Cr1-x Fe x (OH)(3) oxidation by oxygen in the dark, considering pH, Fe/Cr ratio, and the presence of siderophores. Results show that pH and Fe/Cr ratio linearly affect the Cr(III) oxidation rate, while the siderophore promotes the formation of soluble organically complexed Cr(III) and enhances its oxidation by oxygen. This study emphasizes the importance of oxygen and its interaction with siderophores in the oxidation of Cr(III)-Fe(III) hydroxides.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Harald Nothaft, Xiaoming Bian, Asif Shajahan, William G. Miller, David T. Bolick, Richard L. Guerrant, Parastoo Azadi, Kenneth K. S. Ng, Christine M. Szymanski
Summary: The study identified C. jejuni isolates expressing a hexasaccharide lacking the glucose branch, caused by a missing aspartate in the DXDD motif of the glucosyltransferase PglI. Mouse vaccination with E. coli expressing the full-length heptasaccharide demonstrated reduction in C. jejuni colonization and weight loss. The study suggests the importance of considering glucose inclusion in N-glycan-based vaccines for preventing campylobacteriosis.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biology
Stephanie Peters, Ben Pascoe, Zuowei Wu, Sion C. Bayliss, Ximin Zeng, Adam Edwinson, Sakteesh Veerabadhran-Gurunathan, Selina Jawahir, Jessica K. Calland, Evangelos Mourkas, Robin Patel, Terra Wiens, Marijke Decuir, David Boxrud, Kirk Smith, Craig T. Parker, Gianrico Farrugia, Qijing Zhang, Samuel K. Sheppard, Madhusudan Grover
Summary: Researchers used whole-genome sequencing and phenotypic analysis to identify potential genetic factors linked to irritable bowel syndrome caused by Campylobacter jejuni infection. They found that specific genetic variations in bacterial stress response, adhesion protein, and core biosynthetic pathway genes may contribute to the development of post-infection irritable bowel syndrome (PI-IBS). The study demonstrated that certain Campylobacter genotypes exhibit increased virulence in vitro, shedding light on the complex host-pathogen interactions underlying this condition.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Robert Zscherp, Janetta Coetzee, Johannes Vornweg, Joerg Grunenberg, Jennifer Herrmann, Rolf Mueller, Philipp Klahn
Summary: The design, synthesis, and biological evaluation of the artificial enterobactin analogue Ent(KL) and its fluorophore-conjugates are described in this study. Ent(KL) can promote the growth of Gram-negative pathogens and has a iron binding constant similar to natural enterobactin. The conjugates are recognized by outer membrane siderophore receptors and can deliver molecular cargos to bacterial cells.
Article
Chemistry, Inorganic & Nuclear
Pawel Jewula, Mickael Grandmougin, Melanie Choppin, Anna Maria Chiara Tivelli, Agnese Amati, Yoann Rousselin, Lydia Karmazin, Jean-Claude Chambron, Michel Meyer
Summary: Six- and seven-membered cyclic hydroxamic acids are commonly found in siderophores and can act as terminal binding units. However, the coordination chemistry of these ligands with Fe3+, the target metal ion of siderophores, has not been previously investigated. In this study, four complexes were synthesized and studied, and X-ray diffraction analysis showed that the AZEPO(-) complexes had better minimized interligand repulsion energies and shorter Fe-O bond distances compared to the PIPO complexes.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Afruja Khan, Avijit Sardar, Pradip K. Tarafdar, Amirul I. Mallick
Summary: Campylobacter jejuni uses naturally secreted outer membrane vesicles (OMVs) to deliver toxins to target cells, relying on their biophysical and compositional attributes. The study reveals that OMVs uptake is mediated by cytosolic and multiple endocytic processes, showing target cell-specific preferential uptake.
ACS INFECTIOUS DISEASES
(2023)
Article
Biochemical Research Methods
Jean L. Whittingham, Shumpei Hanai, James A. Brannigan, William T. Ferreira, Eleanor J. Dodson, Johan P. Turkenburg, Jared Cartwright, Simon M. Cutting, Anthony J. Wilkinson
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2020)
Article
Biophysics
Katherine A. Edmonds, Yifan Zhang, Daniel J. Raines, Anne-K Duhme-Klair, David P. Giedroc
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Chemistry, Medicinal
Thomas J. Sanderson, Conor M. Black, James W. Southwell, Ellis J. Wilde, Apurva Pandey, Reyme Herman, Gavin H. Thomas, Eszter Boros, Anne-Kathrin Duhme-Klair, Anne Routledge
ACS INFECTIOUS DISEASES
(2020)
Article
Biochemical Research Methods
Paul S. Bond, Keith S. Wilson, Kevin D. Cowtan
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Biochemical Research Methods
Nicholas G. S. McGregor, Johan P. Turkenburg, Kristian B. R. Morkeberg Krogh, Jens Erik Nielsen, Marta Artola, Keith A. Stubbs, Herman S. Overkleeft, Gideon J. Davies
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Review
Chemistry, Medicinal
James W. Southwell, Conor M. Black, Anne-Kathrin Duhme-Klair
Summary: Trojan Horse antibacterials, also known as antibacterial siderophore conjugates, have attracted increasing attention in the field of antibiotics due to their potential to exploit specific pathways in bacteria for active iron uptake and bypass membrane-associated resistance mechanisms. Evaluating the bacterial uptake and identifying the pathways are crucial for improving the design of such antibacterials.
Article
Multidisciplinary Sciences
Olga Moroz, Elena Blagova, Edward Taylor, Johan P. Turkenburg, Lars K. Skov, Garry P. Gippert, Kirk M. Schnorr, Li Ming, Liu Ye, Mikkel Klausen, Marianne T. Cohn, Esben G. W. Schmidt, Soren Nymand-Grarup, Gideon J. Davies, Keith S. Wilson
Summary: Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall, and enzymes from GH25 family have been identified and characterized for potential commercial application in animal feed industry. The crystal structure of the A. alcalophilum enzyme at atomic resolution was reported, showing promise for processing bacterial debris in the animal gut.
Article
Biochemical Research Methods
Olga Moroz, Elena Blagova, Andrey A. Lebedev, Filomeno Sanchez Rodriguez, Daniel J. Rigden, Jeppe Wegener Tams, Reinhard Wilting, Jan Kjolhede Vester, Elena Longhin, Gustav Hammerich Hansen, Kristian Bertel Romer Morkeberg Krogh, Roland A. Pache, Gideon J. Davies, Keith S. Wilson
Summary: Beta-galactosidases can catalyze the hydrolysis of lactose into galactose and glucose, as well as the formation of galactooligosaccharides by transglycosylation. The structure of a multidomain beta-galactosidase from Bifidobacterium bifidum revealed possible mechanisms for switching between lactose hydrolysis and transgalactosylation activities. Engineering opportunities for selective modification of beta-galactosidases for industrial purposes may be possible in the future.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Natalia G. Baranska, Alison Parkin, Anne -K. Duhme-Klair
Summary: We report the electrochemical study of iron(III) catecholate siderophores using a boron-doped diamond working electrode. Our results reveal the formation of coordinatively unsaturated tetracoordinate complexes of iron(III) azotochelin, contrary to other siderophore complexes which favor hexacoordinate environment. The reduction potential of iron(III) azotochelin shows notable pH and buffer composition dependence. The study also extends to the investigation of iron(III) hexadentate siderophores.
INORGANIC CHEMISTRY
(2022)
Article
Microbiology
Adam M. Hughes, John F. Darby, Eleanor J. Dodson, Samuel J. Wilson, Johan P. Turkenburg, Gavin H. Thomas, Anthony J. Wilkinson
Summary: Peptide transporters play important roles in nutrition and cell signaling in Bacillus subtilis. Crystal structures of the substrate binding proteins OppA and DppE have revealed their specific binding modes and substrates, shedding light on the uptake and recycling of peptides and their significance in bacterial adaptation and development.
Article
Biochemical Research Methods
Elena V. Blagova, Alex H. Miller, Megan Bennett, Rosalind L. Booth, Eleanor J. Dodson, Anne-Kathrin Duhme-Klair, Keith S. Wilson
Summary: Siderophore-binding proteins from two thermophilic bacteria, Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius, were identified as homologues of the well characterized protein CjCeuE from Campylobacter jejuni. The crystal structures of these proteins were determined and their thermostability was found to be higher than CjCeuE. The homologues also showed enhanced tolerance to the organic solvent dimethylformamide (DMF). These thermophilic homologues offer advantages in the development of artificial metalloenzymes.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Maryam Nazari Haghighi Pashaki, Tae-Kyu Choi, Egmont J. Rohwer, Thomas Feurer, Anne-Kathrin Duhme-Klair, Wojciech Gawelda, Andrea Cannizzo
Summary: Photo-induced oxidation-enhancement in biomimetic bridged Ru(ii)-Mo(vi) photo-catalyst is unexpectedly photo-activated in ps timescales, where one-photon absorption generates an excited state activating both photo-oxidized and photo-reduced catalytic centres independently.
CHEMICAL COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Rosalind L. Booth, Gideon Grogan, Keith S. Wilson, Anne-Kathrin Duhme-Klair
RSC CHEMICAL BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Ben Coulson, Mark Isaacs, Leonardo Lari, Richard E. Douthwaite, Anne-K. Duhme-Klair
CHEMICAL COMMUNICATIONS
(2019)