Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Seongjun So, Yoonkyung Park, Soon Suk Kang, Jongsuk Han, Jeong Hye Sunwoo, Whanseo Lee, Jin Kim, Eun Ah Ye, Jae Yong Kim, Hungwon Tchah, Eunju Kang, Hun Lee
Summary: Corneal endothelial cells (CECs) cannot proliferate or recover, causing decreased cell density and loss of pump/barrier function. The study investigated the efficacy and safety of transplanting induced pluripotent stem cell (iPSC)-derived CECs into a corneal endothelial dysfunction (CED) rabbit model. Results showed successful differentiation of iPSCs into CECs and improved corneal transparency after transplantation. iPSC-derived CECs may be a promising cellular resource for treating CED.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Xiao Yu Ng, Gary S. L. Peh, Gary Hin-Fai Yam, Hwee Goon Tay, Jodhbir S. Mehta
Summary: Corneal endothelial dysfunction is a major cause of corneal blindness, and the current treatment option is corneal transplantation. However, there is a shortage of suitable donor graft material, leading to the exploration of stem cell-based regenerative medicine using induced pluripotent stem cells (iPSCs). This review discusses the progress and challenges in inducing iPSCs into corneal endothelial cells (CECs), as well as the potential risks and solutions for clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Baoqi Sun, Timur Bikkuzin, Xuran Li, Yan Shi, Hong Zhang
Summary: CE dysfunction can be treated through corneal transplantation, but issues such as global cornea shortage and graft failure still exist, highlighting the need for finding an alternative to CE.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Biotechnology & Applied Microbiology
Kangli Cui, Tingwei Chen, Yujuan Zhu, Yang Shi, Yaqiong Guo, Jianhua Qin
Summary: This study established a new model system by utilizing human-induced pluripotent stem cells to create placenta-like organoids with vascular-like structures in a three-dimensional culture. The resulting tissue resembled first-trimester human placental development and exhibited complex placental components and secretory function. This model system opens new avenues for studying normal early human placental development and its disease states.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Helena Isla-Magrane, Anna Veiga, Jose Garcia-Arumi, Anna Duarri
Summary: A two-step method was established to obtain self-organized multizone ocular progenitor cells (mzOPCs) from 2D hiPSC cultures within three weeks, which were then grown in suspension to create 3D multiocular organoids. These organoids exhibited correct layering of retinal regions, resembled transparent ocular-surface epithelium in the corneal regions, and contained interactions between different cell types and regions of the eye system. This approach demonstrated the advantages of the combined 2D-3D construction tissue model in creating a more ocular native-like cellular environment for modeling eye development and disease.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Shuo Cao, Xinyue Gao, Fangyuan Liu, Yanglin Chen, Qin Na, Qiaoqiao Meng, Peng Shao, Chen Chen, Yongli Song, Baojiang Wu, Xihe Li, Siqin Bao
Summary: This study derived iPSCs from fibroblasts of a patient with acute myelitis and compared them to iPSCs from normal individuals. The patient-derived iPSCs showed attenuated potential for ectoderm differentiation, which was found to be related to the Wnt signaling pathway. This study provides a novel approach to investigating the pathogenesis of acute myelitis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Cell Biology
Pyry Gronroos, Tanja Ilmarinen, Heli Skottman
Summary: This study successfully generated CEnC-like cells from human pluripotent stem cells using a defined differentiation method involving small molecule induction and retinoic acid. The induced cells exhibited morphological characteristics and expression of CEnC markers, demonstrating the potential of this method for regenerative medicine applications in corneal endothelial cells.
Article
Chemistry, Medicinal
Daichi Onozato, Isamu Ogawa, Yuriko Kida, Shota Mizuno, Tadahiro Hashita, Takahiro Iwao, Tamihide Matsunaga
Summary: Novel budding-like organoids derived from human iPS cells exhibit characteristics of mature small intestine and high expression of drug transporters, showing potential for disease modeling and drug screening applications.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Cell & Tissue Engineering
Finn J. Hawkins, Shingo Suzuki, Mary Lou Beermann, Cristina Barilla, Ruobing Wang, Carlos Villacorta-Martin, Andrew Berical, J. C. Jean, Jake Le Suer, Taylor Matte, Chantelle Simone-Roach, Yang Tang, Thorsten M. Schlaeger, Ana M. Crane, Nadine Matthias, Sarah X. L. Huang, Scott H. Randell, Joshua Wu, Jason R. Spence, Gianni Carraro, Barry R. Stripp, Andras Rab, Eric J. Sorsher, Amjad Horani, Steven L. Brody, Brian R. Davis, Darrell N. Kotton
Summary: The directed differentiation of human iPSCs into airway basal cells (iBCs) demonstrates the functional characteristics of airway basal cells and can be used to model characteristic changes in airway diseases.
Article
Multidisciplinary Sciences
Yanxing Wei, Tianyu Wang, Lishi Ma, Yanqi Zhang, Yuan Zhao, Kathryn Lye, Lu Xiao, Chunlin Chen, Zhijian Wang, Yanlin Ma, Xiaohua Zhou, Fei Sun, Weili Li, Caroline Dunk, Siliang Li, Andras Nagy, Yanhong Yu, Guangjin Pan, Stephen J. Lye, Yongli Shan
Summary: This passage discusses the successful generation of trophoblast stem cells from primed human pluripotent stem cells, with BMP4 playing a significant role in the process. The derived TSCs are similar to blastocyst-derived hTSCs in various characteristics, and the chromatin accessibility dynamics and histone modifications involved in specifying hPSC-derived TSCs are defined. Additionally, knockout of H3K27 methyltransferases (EZH1/2) is shown to increase the efficiency of hTSC derivation from primed hPSCs.
Article
Cell Biology
Taiki Satoh, Marcelo A. S. Toledo, Janik Boehnke, Kathrin Olschok, Niclas Flosdorf, Katrin Goetz, Caroline Kuestermann, Stephanie Sontag, Kristin Sere, Steffen Koschmieder, Tim H. Bruemmendorf, Nicolas Chatain, Yoh-ichi Tagawa, Martin Zenke
Summary: Dendritic cells (DC) are professional antigen-presenting cells that can be divided into different subsets, with the DC3 subset having proinflammatory properties. Human iPS cell-derived DC3 exhibit similar characteristics to blood DC3 in vitro. The JAK2 V617F mutation enhances DC3 production and biases towards erythrocytes and megakaryocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Ophthalmology
Mohammed Ziaei, Akilesh Gokul, Hans Vellara, Dipika Patel, Charles N. J. McGhee
Summary: This study found that in eyes with keratoconus, epithelial debridement increased the anterior corneal prolateness and tended to increase astigmatism while shifting its axis towards the against-the-rule orientation.
CANADIAN JOURNAL OF OPHTHALMOLOGY-JOURNAL CANADIEN D OPHTALMOLOGIE
(2021)
Review
Urology & Nephrology
Richard W. Naylor, Mychel R. P. T. Morais, Rachel Lennon
Summary: The glomerular basement membrane (GBM) is crucial for kidney filtration, with studies revealing insights into its composition, assembly, developmental transitions, and filtration role. Defects in GBM components are associated with hereditary and acquired diseases, with current treatments focused on reducing intraglomerular pressure and addressing underlying causes. Therapies targeting GBM maintenance and repair could lead to new treatments for GBM-associated diseases.
NATURE REVIEWS NEPHROLOGY
(2021)
Article
Ophthalmology
Akilesh Gokul, Mohammed Ziaei, Jeremy J. Mathan, Jina Han, Stuti L. Misra, Dipika Patel, Charles N. J. McGhee
Summary: This study investigated the epidemiologic, demographic, and clinical characteristics of keratoconus patients managed by optometrists in New Zealand. The results showed that keratoconus is relatively common in New Zealand, with most patients having mild to moderate disease. However, Maori and Pacific Peoples may have greater disease severity.
Article
Anatomy & Morphology
Peter W. Hadden, Dane A. Gerneke, Charles N. J. McGhee, Jie Zhang
Summary: In this study, the skeletal elements present in the penguin eye and orbit were imaged using macro photographs and micro-CT, revealing that the penguin scleral ring not only maintains the shape of the eye but also provides protection and a site of insertion for rectus muscles. The extreme variability in the os opticus suggests that it is not essential to normal function.
JOURNAL OF MORPHOLOGY
(2021)
Article
Ophthalmology
Jay J. Meyer, Charlotte A. Jordan, Dipika V. Patel, Akilesh Gokul, Simon J. Dean, Suheb Ahmed, Shanu Subbiah, Charles N. J. McGhee
Summary: This study demonstrates that CXL is effective in reducing maximum keratometry and maintaining better visual acuity for at least 5 years in patients with progressive keratoconus. However, complications may occur in some participants and require careful consideration.
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Review
Ophthalmology
Aaron P. C. Ong, Jie Zhang, Andrea L. Vincent, Charles N. J. McGhee
Summary: Megalocornea and anterior megalophthalmos disorders are characterized by a corneal diameter > 12.5 mm, often congenital and associated with syndromes. Megalocornea spectrum disorders are distinguished from keratoglobus by their typically progressive nature. Surgical management can be challenging due to specific anatomical features.
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Article
Ophthalmology
Jie Zhang, Mohammed Ziaei, James McKelvie, Charles N. J. McGhee, Dipika Patel
Summary: The study investigated the in vivo biocompatibility of an acellular collagen-based scaffold for anterior lamellar keratoplasty in a rabbit model. Results showed that the scaffold supported corneal re-epithelialization without signs of rejection but further modifications are needed for better integration.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Ye Li, Akilesh Gokul, Charles McGhee, Mohammed Ziaei
Summary: The study found that both devices showed good repeatability but poor agreement in biometric measurements in keratoconus patients. There was no significant difference in repeatability between eyes with or without prior crosslinking or contact lens wear, but the interchangeable use of the two devices is not recommended.
Article
Multidisciplinary Sciences
Ye Li, Akilesh Gokul, Charles McGhee, Mohammed Ziaei
Summary: This study investigated the repeatability of corneal thickness and epithelial thickness measurements in keratoconus patients using AS-OCT. The results showed that AS-OCT provides reliable measurements in both central and peripheral cornea, and a history of CXL has no significant impact on the repeatability of measurements.
Article
Multidisciplinary Sciences
Alexander Buckenham Boyle, Soobin Namkung, William Shew, Akilesh Gokul, Charles N. J. McGhee, Mohammed Ziaei
Summary: This study assessed the agreement and repeatability of horizontal visible iris diameter (HVID) or white-to-white (WTW) measurements between four imaging modalities. The results indicated significant differences in measurements among the devices, with Galilei G2 showing the highest repeatability and Orbscan IIz the lowest.
Article
Ophthalmology
Xu Chen, Jiaxin Zhao, Katja C. Iselin, Davide Borroni, Davide Romano, Akilesh Gokul, Charles N. J. McGhee, Yitian Zhao, Mohammad-Reza Sedaghat, Hamed Momeni-Moghaddam, Mohammed Ziaei, Stephen Kaye, Vito Romano, Yalin Zheng
Summary: The study evaluated the accuracy of convolutional neural networks technique in detecting keratoconus using colour-coded corneal maps obtained by a Scheimpflug camera. Results showed that the CNN model has excellent detection performance and can accurately differentiate between different severities of the disease, with potential for screening and management of keratoconus.
BMJ OPEN OPHTHALMOLOGY
(2021)
Article
Multidisciplinary Sciences
Ioannis N. Petropoulos, Kathryn C. Fitzgerald, Jonathan Oakley, Georgios Ponirakis, Adnan Khan, Hoda Gad, Pooja George, Dirk Deleu, Beatriz G. Canibano, Naveed Akhtar, Ashfaq Shuaib, Ahmed Own, Taimur Malik, Daniel B. Russakoff, Joseph L. Mankowski, Stuti L. Misra, Charles N. J. McGhee, Peter Calabresi, Shiv Saidha, Saadat Kamran, Rayaz A. Malik
Summary: Axonal loss is the main determinant of disease progression in multiple sclerosis, and corneal confocal microscopy was found to be useful in detecting corneal axonal loss in different courses of MS. The study confirmed significant corneal axonal loss in MS patients, including those with clinically isolated syndrome, using two independent segmentation methods. The corneal nerve parameters were comparably reduced in different courses of MS, with excellent reproducibility between the algorithms.
SCIENTIFIC REPORTS
(2021)
Article
Pediatrics
Richard W. Naylor, Elizabeth Watson, Samantha Williamson, Rebecca Preston, J. Bernard Davenport, Nicole Thornton, Martin Lowe, Maggie Williams, Rachel Lennon
Summary: A novel homozygous truncating variant in CD151 was identified in a young child with nail dystrophy and persistent urinary tract infections presenting with nephrotic-range proteinuria. The imaging of patient kidney tissue revealed thickening of GBM and podocyte effacement. Functional testing in zebrafish confirmed that depletion of cd151 caused proteinuria, indicating the association of CD151 variant with nephrotic-range proteinuria and microscopic hematuria.
PEDIATRIC NEPHROLOGY
(2022)
Article
Multidisciplinary Sciences
Ranjay Jayadev, Mychel R. P. T. Morais, Jamie M. Ellingford, Sandhya Srinivasan, Richard W. Naylor, Craig Lawless, Anna S. Li, Jack F. Ingham, Eric Hastie, Qiuyi Chi, Maryline Fresquet, Nikki-Maria Koudis, Huw B. Thomas, Raymond T. O'Keefe, Emily Williams, Antony Adamson, Helen M. Stuart, Siddharth Banka, Damian Smedley, David R. Sherwood, Rachel Lennon
Summary: By utilizing bioinformatic and in vivo approaches, we have identified a network of proteins involved in basement membrane regulation and function. This study highlights the complexity of basement membranes and their impact on human health.
Article
Urology & Nephrology
Richard W. Naylor, Emmanuel Lemarie, Anthony Jackson-Crawford, J. Bernard Davenport, Aleksandr Mironov, Martin Lowe, Rachel Lennon
Summary: Zebrafish is a valuable model for studying kidney dysfunction. Researchers in this study created a transgenic zebrafish line that can quantitatively measure proteinuria, providing a new tool for screening drugs to alleviate proteinuria.
KIDNEY INTERNATIONAL
(2022)