Article
Oncology
Jonathan Dauve, Nicolas Belloy, Romain Rivet, Nicolas Etique, Pierre Nizet, Katarzyna Pietraszek-Gremplewicz, Konstantina Karamanou, Manuel Dauchez, Laurent Ramont, Stephane Brezillon, Stephanie Baud
Summary: This study investigated the interactions between lumican-derived peptides and MMP-14 using molecular modeling. The results showed that different types of lumican-derived peptides have varying effects on MMP-14 activity, inhibiting melanoma cell migration and proliferation.
Article
Biochemistry & Molecular Biology
Jin-Young Lee, Da-Ae Kim, Eun-Young Kim, Eun-Ju Chang, So-Jeong Park, Beom-Jun Kim
Summary: Lumican inhibits osteoclastogenesis by suppressing Akt activity, leading to an osteoprotective role by simultaneously increasing bone formation and decreasing bone resorption, suggesting it represents a dual-action therapeutic target for osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Romain Rivet, Rajas Mallenahalli Rao, Pierre Nizet, Nicolas Belloy, Louise Huber, Manuel Dauchez, Laurent Ramont, Stephanie Baud, Stephane Brezillon
Summary: This study characterized the structure and dynamics of four SLRPs using in silico 3D modeling, and evaluated their capacity to bind to MMP-14 and regulate its activity. The results demonstrated that each SLRP exhibited significant inhibition of MMP-14 activity, which was related to the core protein conformation as well as the glycan chain structures and dynamics.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Konstantina Karamanou, Marco Franchi, Isabelle Proult, Romain Rivet, Demitrios Vynios, Stephane Brezillon
Summary: Lumican inhibits melanoma cell lung metastasis by targeting Snail-induced migration, affecting various signaling pathways and molecules, and suppressing tumor cell invasion. This study highlights the potential significance of a lumican-based strategy in melanoma treatment by targeting Snail-induced metastasis.
Article
Environmental Sciences
Po-Chung Ju, Yung-Chuan Ho, Pei-Ni Chen, Hsiang-Lin Lee, Szu-Yu Lai, Shun-Fa Yang, Chao-Bin Yeh
Summary: Kaempferol inhibits the invasive properties of human HCC cells by targeting MMP-9 and Akt pathways, showing potential as an adjuvant therapeutic agent for the treatment of human HCC cells.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Neurosciences
Juan Carlos Martinez-Camarillo, Christine K. Spee, Gloria Paulina Trujillo-Sanchez, Anthony Rodriguez, David R. Hinton, Alessandra Giarola, Victor Pikov, Arun Sridhar, Mark S. Humayun, Andrew C. Weitz
Summary: Modulating ocular sympathetic activity can affect the progression of age-related macular degeneration. Surgical and pharmacological inhibition of ocular sympathetic activity can inhibit the development of CNV.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Cell Biology
Yazhen Xu, Bingyu Guo, Xiaoyan Liu, Kai Tao
Summary: miR-34a inhibits melanoma growth by targeting ZEB1, suggesting a potential novel target for melanoma treatment.
Article
Biochemistry & Molecular Biology
Akos Janos Varga, Istvan Balazs Nemeth, Lajos Kemeny, Janos Varga, Laszlo Tiszlavicz, Dinesh Kumar, Steven Dodd, Alec W. M. Simpson, Tunde Buknicz, Rob Beynon, Deborah Simpson, Tibor Krenacs, Graham J. Dockray, Andrea Varro
Summary: Micro-environmental factors play an important role in the progression of melanoma. Gastrin concentration is significantly associated with melanoma thickness and metastasis. Gastrin promotes migration and invasion of melanoma cells through CCK2 receptors, and also affects adjacent stromal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Cheng-Chen Huang, Chun-Wen Su, Po-Hui Wang, Yen-Ting Lu, Yu-Ting Ho, Shun-Fa Yang, Chung-Han Hsin, Chiao-Wen Lin
Summary: This study found that dihydromyricetin (DHM) inhibited the migration and invasion of nasopharyngeal carcinoma (NPC) cells by suppressing the expression of matrix metalloproteinases-2 (MMP-2) via down regulating the extracellular signal-regulated kinase 1 and 2 (ERK1/2) signaling pathway.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Oncology
Christopher Groth, Ludovica Arpinati, Merav E. Shaul, Nina Winkler, Klara Diester, Nicolas Gengenbacher, Rebekka Weber, Ihor Arkhypov, Samantha Lasser, Vera Petrova, Hellmut G. Augustin, Peter Altevogt, Jochen Utikal, Zvi G. Fridlender, Viktor Umansky
Summary: Myeloid-derived suppressor cells (MDSC) are a heterogeneous myeloid cell population expanded in tumor-bearing hosts and contribute to immunosuppression, making them a valuable therapeutic target. In melanoma, both polymorphonuclear (PMN) and monocytic (M) MDSC subsets have comparable immunosuppressive activities, with PMN-MDSC recruitment mediated by the CXCR2/CXCL1 axis. Inhibiting CXCR2 decreases PMN-MDSC infiltration, improves survival, and reduces metastasis, suggesting a critical role of PMN-MDSC in suppressing the NK cell-mediated anti-tumor response.
Article
Oncology
Longhui Ruan, Weifeng Liu, Yanhui Yang, Zhijie Chu, Cheng Yang, Tianbao Yang, Junjun Sun
Summary: The study reveals that low expression of TRIM16 is associated with better prognosis in colorectal cancer, and its overexpression can notably inhibit cancer cell metastasis. The inhibition effect of TRIM16 on cancer metastasis is dependent on the ubiquitination of Snail protein, a key regulator of the epithelial-mesenchymal transition process, ultimately suppressing cancer progression.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Oncology
Roberto Coppo, Francesca Orso, Federico Virga, Alberto Dalmasso, Desiree Baruffaldi, Lei Nie, Fabiana Clapero, Daniela Dettori, Lorena Quirico, Elena Grassi, Paola Defilippi, Paolo Provero, Donatella Valdembri, Guido Serini, Mehran M. Sadeghi, Massimiliano Mazzone, Daniela Taverna
Summary: ESDN plays a critical role in melanoma progression by regulating the expression of E-selectin in endothelial cells, affecting melanoma cell adhesion, extravasation, and metastasis formation. The study proposes a protective role for ESDN in melanoma spread and highlights its therapeutic potential.
Article
Chemistry, Multidisciplinary
Zhongying Du, Zhesheng He, Jiadong Fan, Yangao Huo, Bo He, Yueran Wang, Qianqian Sun, Wenchao Niu, Wencong Zhao, Lina Zhao, Peng Cao, Kai Cao, Dongfang Xia, Qing Yuan, Xing-Jie Liang, Huaidong Jiang, Yong Gong, Xueyun Gao
Summary: Studies have shown that gold clusters inhibit human thioredoxin reductase, leading to tumor cell apoptosis, and may serve as potential drugs for cancer treatment. This study reveals the structural mechanism of gold inhibiting enzyme activity and provides insights for the design of novel anti-tumor nanodrugs.
Article
Multidisciplinary Sciences
Ewa Mrowczynska, Ewa Mazurkiewicz, Antonina Joanna Mazur
Summary: This paper introduces a gelatin zymography assay for analyzing the gelatinase activity of melanoma cells, and describes the experimental procedure in detail. The method is simple, sensitive, and cost-effective, allowing for the detection of both active and inactive forms of metalloproteases.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Environmental Sciences
Chung-Yuan Lee, Yung-Chuan Ho, Chiao-Wen Lin, Min-Chieh Hsin, Po-Hui Wang, Ya-Cheng Tang, Shun-Fa Yang, Yi-Hsuan Hsiao
Summary: The synthetic curcumin analog, EF-24, has been shown to have enhanced bioavailability and stronger anti-inflammatory and anti-cancer activity compared to curcumin. This study investigated the effects of EF-24 on TPA-induced cellular migration of cervical cancer and found that EF-24 significantly reduced cellular migration and invasion. Further analysis revealed that EF-24 modulated MMP-9 expression by inhibiting the phosphorylation of the p38 signaling pathway. These findings suggest that EF-24 may have potential as a chemopreventive agent for cervical cancer.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Oncology
Izabela Papiewska-Pajak, Patrycja Przygodzka, Damian Krzyzanowski, Kamila Soboska, Izabela Szulc-Kielbik, Olga Stasikowska-Kanicka, Joanna Boncela, Malgorzata Wagrowska-Danilewicz, M. Anna Kowalska
Summary: Understanding the role of extracellular vesicles (EVs) in cancer progression is crucial, as EVs released from cells with migratory properties contain different factors compared to EVs from original tumor cells. These EVs could potentially serve as biomarkers to denote the stage of colorectal cancer (CRC) and be targeted to prevent cancer progression.
Article
Oncology
Patrycja Przygodzka, Ewelina Sochacka, Kamila Soboska, Marcin Pacholczyk, Izabela Papiewska-Pajak, Tomasz Przygodzki, Przemyslaw Plocinski, Steven Ballet, An De Prins, Joanna Boncela
Summary: The study found elevated expression of NMU and NMUR2 in CRC tissue samples and variable expression in CRC cell lines. It was shown for the first time that NMUR2 activation induces signaling in CRC cells, and NMU increases motility and invasiveness in NMUR2-positive CRC cells, as well as promoting prometastatic integrin receptor subunit expression.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jolanta Kryczka, Jakub Kryczka, Lukasz Janczewski, Anna Gajda, Andrzej Fraczyk, Joanna Boncela, Beata Kolesinska, Ewa Brzezianska-Lasota
Summary: One of the main challenges in the treatment of non-small-cell lung cancer is the development of resistance to cisplatin-based chemotherapy. This study investigates the potential of isothiocyanates (ITCs) and the ALDH inhibitor disulfiram (DSF) as adjuvants to cisplatin chemotherapy. The results show that certain ITCs and DSF can reduce the cisplatin tolerance of resistant lung cancer cells, suggesting their potential in overcoming drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jakub Kryczka, Joanna Boncela
Summary: Different drug combinations, including irinotecan, are important in treating colorectal cancer (CRC). However, chemotherapy often leads to drug resistance. This study compared mRNA expression profiles of CRC cell lines and patient samples to select new candidate genes for irinotecan resistance. The results showed that in vitro models have limitations and cannot fully reflect the complex drug resistance network observed in patients.
Article
Cell Biology
Romain Rivet, Rajas Mallenahalli Rao, Pierre Nizet, Nicolas Belloy, Louise Huber, Manuel Dauchez, Laurent Ramont, Stephanie Baud, Stephane Brezillon
Summary: This study characterized the structure and dynamics of four SLRPs using in silico 3D modeling, and evaluated their capacity to bind to MMP-14 and regulate its activity. The results demonstrated that each SLRP exhibited significant inhibition of MMP-14 activity, which was related to the core protein conformation as well as the glycan chain structures and dynamics.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Patrycja Przygodzka, Kamila Soboska, Ewelina Sochacka, Marcin Pacholczyk, Marcin Braun, Hassan Kassassir, Izabela Papiewska-Pajak, Michal Kielbik, Joanna Boncela
Summary: The study revealed that NMU levels are elevated in human CRC compared to normal tissue, with high NMUR1 expression correlating with shorter overall survival in CRC patients. NMUR1 expression was identified in macrophages, endothelial cells, platelets, and platelet microparticles, with NMU-stimulated macrophages increasing the migratory potential of CRC cells. Additionally, NMU activation induced pro-metastatic phenotypes in macrophages and endothelial cells, changing their secretome.
CELL COMMUNICATION AND SIGNALING
(2022)
Review
Cell & Tissue Engineering
Charlie Colin-Pierre, Oussama El Baraka, Louis Danoux, Vincent Bardey, Valerie Andre, Laurent Ramont, Stephane Brezillon
Summary: This article reviews the structure, classification, and role of heparan sulfate proteoglycans (HSPGs) in stem cell fate regulation. Specifically, it focuses on hair follicle stem cell fate and the potential use of HSPGs to treat hair loss from dermatological and cosmeceutical perspectives.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Charlie Colin-Pierre, Valerie Untereiner, Ganesh D. Sockalingum, Laurent Ramont, Stephane Brezillon
Summary: The expression of glypicans in different hair follicle (HF) compartments was investigated using infrared spectral imaging (IRSI) for the first time, showing the distribution changes of GPC4 and GPC6 in HFs during different phases of the hair growth cycle. Western blot assays confirmed these findings. IRSI demonstrated its ability to identify the location of proteins, proteoglycans, glycosaminoglycans, and sulfated glycosaminoglycans in HFs in a label-free manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Agnieszka Wosiak, Dagmara Szmajda-Krygier, Jacek Pietrzak, Joanna Boncela, Ewa Balcerczak
Summary: Changes in the expression of SMAD4 gene may affect the sensitivity and resistance of colorectal cancer cells to 5-FU.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Kamila Baran, Joanna Wasko, Jakub Kryczka, Joanna Boncela, Slawomir Jablonski, Beata Kolesinska, Ewa Brzezianska-Lasota, Jacek Kordiak
Summary: A comparative analysis using high-performance liquid chromatography coupled to mass spectrometry was conducted to identify key proteins in serum exosomes that play a crucial role in the development and metastasis of non-small lung cancer. The study identified 150 NSCLC proteins involved in osmoregulation, cell adhesion, cell motility, and differentiation, with three proteins showing significant involvement in cancer-associated fibroblasts and tumor-associated macrophages infiltration processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Critical Care Medicine
Auguste Dargent, Hugo Dumargne, Marie Labruyere, Stephane Brezillon, Sylvie Brassart-Pasco, Mathieu Blot, Pierre-Emmanuel Charles, Isabelle Fournel, Jean-Pierre Quenot, Marine Jacquier
Summary: This article aims to review the literature and summarize the current knowledge along these specific axes, as well as methodological aspects related to interstitium exploration.
JOURNAL OF INTENSIVE CARE
(2023)
Article
Oncology
Jakub Kryczka, Joanna Boncela
Summary: ABCC4 and ABCG2 may have predictive/diagnostic potential in colorectal cancer (CRC) beyond their known functions as drug exporters. By analyzing transcriptomic data of CRC patients, this study identified two subpopulations with high expression levels of ABCC4 or ABCG2, which exhibit distinct gene expression patterns and potential molecular functions.
