期刊
PLANT SCIENCE
卷 242, 期 -, 页码 131-139出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2015.09.008
关键词
MAS; Rice breeding; QTL; SNP; Gene pyramiding
资金
- SATREPS project Development of crop genotypes for midlands and mountain areas of north Vietnam
- SATREPS project Improvement in productivity and yield stability of rice under Kenya's biotic and abiotic stress conditions through tailor-made breeding and development of cultivation methods
- National Bio-Resource Project for Rice
DNA marker-assisted selection (MAS) has become an indispensable component of breeding. Single nucleotide polymorphisms (SNP) are the most frequent polymorphism in the rice genome. However, SNP markers are not readily employed in MAS because of limitations in genotyping platforms. Here the authors report a Golden Gate SNP array that targets specific genes controlling yield-related traits and biotic stress resistance in rice. As a first step, the SNP genotypes were surveyed in 31 parental varieties using the Affymetrix Rice 44K SNP microarray. The haplotype information for 16 target genes was then converted to the Golden Gate platform with 143-plex markers. Haplotypes for the 14 useful allele are unique and can discriminate among all other varieties. The genotyping consistency between the Affymetrix microarray and the Golden Gate array was 92.8%, and the accuracy of the Golden Gate array was confirmed in 3 F-2 segregating populations. The concept of the haplotype-based selection by using the constructed SNP array was proofed. (C) 2015 The Authors. Published by Elsevier Ireland Ltd.
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