Article
Biochemistry & Molecular Biology
Lingjiang Zeng, Qiaozhuo Zhang, Chunxue Jiang, Yueyue Zheng, Youwei Zuo, Jianbo Qin, Zhihua Liao, Hongping Deng
Summary: In this study, A. belladonna plants without anisodamine and scopolamine were generated for the first time by CRISPR/Cas9-based disruption of hyoscyamine 6 beta-hydroxylase (AbH6H). These plants exhibited significantly elevated hyoscyamine production while lacking anisodamine and scopolamine, showing great potential for cost-effective production of hyoscyamine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Diletta Piatti, Riccardo Marconi, Simone Angeloni, Giovanni Caprioli, Filippo Maggi, Emanuele Ribatti, Vittorio Cattaneo, Gianni Sagratini
Summary: This study developed a method to measure the content of scopolamine and hyoscyamine in medicinal plants and analyzed the content of these alkaloids in different parts of four Solanaceae plants.
NATURAL PRODUCT RESEARCH
(2023)
Article
Agricultural Engineering
Junlan Zeng, Muqi Liu, Lingjiang Zeng, Chunxian Yang, Ping He, Min Lin, Zhihua Liao, Fei Qiu
Summary: In this study, the roles of four novel biosynthesis genes in engineering tropane alkaloids (TAs) production were investigated. The results showed that co-overexpression of PYKS and CYP82M3 significantly increased the production of tropinone and tropine. Overexpression of UGT1 or LS greatly enhanced the production of littorine, hyoscyamine, anisodamine, and scopolamine. When all four biosynthesis genes were overexpressed together, the highest production of TAs was achieved, indicating a synergistic effect on TAs' production.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Tengfei Zhao, Junlan Zeng, Mei Yang, Fei Qiu, Yueli Tang, Lingjiang Zeng, Chunxian Yang, Ping He, Xiaozhong Lan, Min Chen, Zhihua Liao, Fangyuan Zhang
Summary: The study investigated the metabolic and developmental roles of the ornithine decarboxylase (ODC) gene in A. belladonna through RNAi-mediated suppression. The results showed that ODC plays a critical role in regulating the biosynthesis of polyamines and medicinal tropane alkaloids, as well as participating in pollen development.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Hannah M. Parks, Maris A. Cinelli, Matthew A. Bedewitz, Josh M. Grabar, Steven M. Hurney, Kevin D. Walker, A. Daniel Jones, Cornelius S. Barry
Summary: This study reveals major changes in the root alkaloid metabolome of Atropa belladonna when pyrrolidine ketide synthase (AbPyKS) is silenced, leading to the discovery of almost 40 pyrrolidine alkaloids that increase. These alkaloids share a core structure formed through the nonenzymatic Mannich-like decarboxylative condensation of the N-methyl Delta(1)-pyrrolinium cation with 2-O-malonylphenyllactate. This research provides insights into the complexity of the A. belladonna root metabolome and lays the foundation for further exploration of the biosynthesis, function, and potential applications of these novel alkaloids.
Article
Biotechnology & Applied Microbiology
Qiaozhuo Zhang, Mengjiao Liang, Junlan Zeng, Chunxian Yang, Jianbo Qin, Wei Qiang, Xiaozhong Lan, Min Chen, Min Lin, Zhihua Liao
Summary: Transgenic homozygous lines of Atropa belladonna with high tolerance to glyphosate and increased production of anticholinergic tropane alkaloids (TAs) were successfully generated. These plants not only tolerated the recommended dose of glyphosate in the field but also showed significantly higher levels of TA production compared to the wild-type plants.
METABOLIC ENGINEERING
(2022)
Review
Plant Sciences
Yuru Wen, Yiran Liao, Yueli Tang, Hongbo Zhang, Jiahui Zhang, Zhihua Liao
Summary: This review discusses the impact of elicitation on the biosynthesis of tropane alkaloids (TAs). It covers single-elicitor treatment, multiple-elicitor treatment, and the combination of elicitation strategy with other strategies. While the synergistic effects of multiple elicitors have been observed, the underlying molecular mechanism remains largely unknown. Further research is needed to effectively implement elicitation strategies in commercial production and explore the integration of elicitation with other strategies.
Article
Plant Sciences
Farnoosh Fattahi, Abdolali Shojaeiyan, Javier Palazon, Elisabeth Moyano, Laura Torras-Claveria
Summary: This study is the first assessment of the effect of beta-CD and Cor elicitors on tropane alkaloid production. In A. acuminata hairy roots, Cor reduced HYO levels but increased SCO content. In A. belladonna hairy roots, all elicitors had a negative effect on both HYO and SCO production.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biotechnology & Applied Microbiology
Juan M. Minoia, Maria E. Villanueva, Guillermo J. Copello, Julian Rodriguez Talou, Alejandra B. Cardillo
Summary: A biocatalytic system was developed for the production of anisodamine and scopolamine, utilizing affinity-immobilized ChBD-H6H which retained catalytic activity in chitin matrices. Enzyme-recycling through adsorption-desorption strategies improved product yields and allowed for the simple and economical reutilization of the enzyme.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Qiaozhuo Zhang, Mengjiao Liang, Yuanyuan Liu, Chunxian Yang, Junlan Zeng, Jianbo Qin, Xiaozhong Lan, Min Lin, Min Chen, Jin Wang, Zhihua Liao
Summary: Through metabolic engineering, new varieties of Atropa belladonna with glyphosate resistance and high-yield scopolamine have been developed, significantly increasing production and holding great value for industrial purposes.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Chemistry, Physical
Fei Qiu, Yijun Yan, Junlan Zeng, Jian-Ping Huang, Lingjiang Zeng, Wei Zhong, Xiaozhong Lan, Min Chen, Sheng-Xiong Huang, Zhihua Liao
Summary: The study on hyoscyamine dehydrogenase revealed the important role of the AbHDH gene in the biosynthesis of hyoscyamine and provided relevant biochemical and metabolic insights. AbHDH was found to enhance the production of hyoscyamine but did not significantly affect the biosynthesis of hyoscyamine, anisodamine, or scopolamine when suppressed.
Article
Biochemistry & Molecular Biology
Fumihito Hasebe, Honoka Yuba, Takashi Hashimoto, Kazuki Saito, Nobutaka Funa, Tsubasa Shoji
Summary: This passage discusses the biosynthesis process of tropane alkaloids in the roots of medicinal plants, outlining the key metabolic enzymes and reaction mechanisms involved. The generation of mutants through genome editing techniques demonstrates the impact of PYKS activity loss on tropane alkaloid metabolism, offering valuable insights into the role of scaffold-forming reaction in this biosynthetic pathway.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Vincent Courdavault, Julien Cassereau, Nicolas Papon
Summary: Tropane alkaloids are difficult to produce in plants, with complex chemical structures, and currently rely heavily on intensive farming of Duboisia shrubs. Adverse factors can affect the production of tropane alkaloids. Recent technological breakthroughs, such as the development of a yeast cell factory, have provided an alternative method for the synthesis of tropane alkaloids.
Article
Plant Sciences
E. Sepehri, B. Hosseini, A. Hedayati
Summary: This study found that iron oxide nano-particles can stimulate the production of tropane alkaloids in Atropa belladonna L., including atropine and scopolamine. The results also showed that the highest phenol and flavonoid content in the peppers was obtained when treated with 250 mg/L elicitor. Furthermore, the highest expression level of the h6h gene was observed in hairy roots stimulated with 750 mg/L iron oxide for 48 hours.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
A. Hedayati, S. Hemmaty, E. Nourozi, A. Amirsadeghi
Summary: This study investigated the effect of yeast extract on the growth, gene expression, and alkaloid production of Atropa belladonna hairy roots. High concentrations of yeast extract were found to effectively increase the tropane alkaloid content in hairy roots, but also reduced growth rate. High levels of scopolamine and atropine were obtained with specific concentrations of yeast extract and exposure times.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)