Review
Biotechnology & Applied Microbiology
Chang Liu, Sijin Li
Summary: This review summarizes the progress made in the past 10 years in type III PKS-catalyzed natural product biosynthesis in microorganisms, with a focus on complete biosynthesis strategies and achievements.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuru Tong, Tianyuan Hu, Lichan Tu, Kang Chen, Tiezheng Liu, Ping Su, Yadi Song, Yuan Liu, Luqi Huang, Wei Gao
Summary: Acyclic terpenes, commonly found in plants, are controlled by different terpene synthases. The study found that terpene synthases from Tripterygium wilfordii exhibited substrate promiscuity and key residues were identified for the generation of diterpene product. Additionally, an engineered yeast producing high levels of nerolidol was constructed for potential applications in microbial fermentation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Plant Sciences
Renu Bisht, Aniket Bhattacharyya, Ankita Shrivastava, Priti Saxena
Summary: Plants produce a variety of secondary metabolites through type III PKSs, which have pharmaceutical, medicinal, and industrial applications, making them important tools for bioengineering. These secondary metabolites affect human health and provide survival advantages for the plant species, showcasing the potential for generating new drugs and modified products through enzyme manipulation.
FRONTIERS IN PLANT SCIENCE
(2021)
Letter
Chemistry, Organic
Bei Zhang, Wenzheng Jin, Yunyun Zhang, Yan Dai, Haohao Li, Xuri Wu, Jun Luo, Yijun Chen
Summary: By genome mining, we have discovered a new type I/type III PKS hybrid called cinnamomycin A-D, which is derived from 3,5-dihydroxybenzoic acid. Through genetic manipulation, enzymatic reaction, and precursor feeding, we proposed the biosynthetic pathway of cinnamomycins.
Article
Chemistry, Organic
Bei Zhang, Wenzheng Jin, Yunyun Zhang, Yan Dai, Haohao Li, Yanzi Sun, Xuri Wu, Jun Luo, Yijun Chen
Summary: Through genome mining, the discovery and characterization of atypical compounds, cinnamomycin A-D, with selective antiproliferative activity. The biosynthetic pathway of cinnamomycins was proposed based on genetic manipulation, enzymatic reaction, and precursor feeding.
Article
Biochemistry & Molecular Biology
Zeping Cai, Xingkun Zhao, Chaoye Zhou, Ting Fang, Guodao Liu, Jiajia Luo
Summary: In this study, we identified 3087 tandem duplicated genes in the S. tora genome and found 14 PKS genes involved in secondary metabolite pathways. Through phylogenetic analysis, we classified the PKS genes into three groups and identified seven genes with high expression in the seeds, which may be associated with the biosynthesis of anthraquinones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agricultural Engineering
Mengjun Xiao, Binbin Wang, Yanan Feng, Peiwen Sun, Mei Rong, Yangyang Liu, Deli Chen, Feifei Lv, Zhihui Gao, Jianhe Wei
Summary: This study reports the isolation and characterization of three type III polyketide synthases with multiple catalytic activities from Aquilaria sinensis, showing that their expression is induced by salt, salicylic acid, or methyl jasmonate. The enzymes exhibit different pH and temperature optima for catalyzing various compounds, and are localized in the cytoplasm and nucleus.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Fumitaka Kudo, Kosuke Kishikawa, Kazuma Tsuboi, Takafusa Kido, Takeo Usui, Junko Hashimoto, Kazuo Shin-ya, Akimasa Miyanaga, Tadashi Eguchi
Summary: In this study, the biosynthetic gene cluster for virustomycin A, an antibiotic produced by Streptomyces graminofaciens A-8890, was identified. The study also demonstrated the exchangeability of the acyltransferase domain between the PKSs involved in FD-891 biosynthesis and the Vsm PKSs. By exchanging the malonyltransferase domain in the loading module of the PKSs, a two-carbon-elongated analog 26-ethyl-FD-891 was successfully produced with potent cytotoxic activity.
Article
Plant Sciences
Dongmei Guo, Hanyan Wang, Shumin Zhang, Ting Lan
Summary: This study provides new insights into the classification, origin, and evolutionary history of the type III polyketide synthase (PKS) supergene family through molecular evolutionary analysis and phylogenomics analysis. The results showed strong positive natural selection in the Pinaceae and Fabaceae lineages, and positively selected sites in the proteins might have driven the functional divergence between the two types. The study also revealed the common occurrence of convergent evolution in enzymes involved in plant flavonoid biosynthesis.
Article
Plant Sciences
Tian Hu, Hao Feng, Yapei Zhao, Wei Yang, Ruiqi Liu, Guanglin Li
Summary: This study investigated the biochemical and functional characteristics of two MTPSL enzymes in moss, revealing differences in monoterpene synthase and sesquiterpene synthase activities. One of the enzymes exhibited antibacterial effects on various bacteria and enhanced resistance in transgenic Arabidopsis thaliana plants. Overall, this study provides new insights into the functions of moss MTPSLs.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Rong Chen, Tianyuan Hu, Ming Wang, Yuhan Hu, Shu Chen, Qiuhui Wei, Xiaopu Yin, Tian Xie
Summary: This study analyzed the metabolites of Curcuma wenyujin's leaf and tuber tissues, identifying 11 curcuminoid metabolites and revealing the biosynthesis pathway. Two candidate type III polyketide synthases (PKSs) were identified, with CwPKS1 being the first one capable of synthesizing hydrogenated derivatives of curcuminoids. Notably, a mutation (G219D) resulted in the complete inactivation of CwPKS1.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Shengling Xie, Lihan Zhang
Summary: Bioinformatics has become an essential tool in natural products research, particularly in converting sequence data of biosynthetic gene clusters into chemical information. Type II polyketide synthase, a well-studied class of non-modular biosynthetic machinery, serves as a model system to demonstrate the applications of bioinformatics in this field. This review highlights the contributions of bioinformatics in enhancing the understanding of type II polyketide synthases in terms of enzymology, evolution, structural prediction of biosynthetic products, genome mining, and global analysis of polyketide products.
Article
Biochemical Research Methods
Matthias Schmidt, Namil Lee, Chunjun Zhan, Jacob B. Roberts, Alberto A. Nava, Leah S. Keiser, Aaron A. Vilchez, Yan Chen, Christopher J. Petzold, Robert W. Haushalter, Lars M. Blank, Jay D. Keasling
Summary: In this study, the influence of 11 different codon variants on heterologous expression was analyzed. The best-performing codon variants resulted in a 50-fold increase in protein levels and successful synthesis of unnatural polyketides in different hosts. Additionally, an online tool for codon optimization was developed.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Microbiology
Qingpei Liu, Dan Zhang, Yao Xu, Shuaibiao Gao, Yifu Gong, Xianhua Cai, Ming Yao, Xiaolong Yang
Summary: This study reported the genome sequence information of the endophytic fungus Preussia isomera and discovered two novel polyketide synthases (PKSs) for synthesizing orsellinic acid derivatives. A non-native PKS was also constructed for the large-scale biosynthesis of orsellinic acid. This work provides valuable insights into the biosynthesis of PKs in P. isomera and offers a new platform for the production of high-level environment-friendly orsellinic acid derivatives and the development of new antimicrobial agents.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Baiying Xing, Jiahui Yu, Changbiao Chi, Xueyang Ma, Qingxia Xu, Annan Li, Yuanjie Ge, Zhengdong Wang, Tan Liu, Hongli Jia, Fuling Yin, Juan Guo, Luqi Huang, Donghui Yang, Ming Ma
Summary: In this study, the structures and functions of pimarane-type diterpene synthases were characterized, revealing that Sat1646 and Stt4548 utilize distinct modes of intermediate stabilization. By comparing the mechanisms of different DTSs, it was inferred that they control the conversion of a single substrate CPP into structurally diverse diterpene products.
COMMUNICATIONS CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sean R. Johnson, Wajid Waheed Bhat, Jacob Bibik, Aiko Turmo, Britta Hamberger, Bjoern Hamberger
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Plant Sciences
Sean R. Johnson, Wajid Waheed Bhat, Radin Sadre, Garret P. Miller, Alekzander Sky Garcia, Bjorn Hamberger
Article
Biology
Dongyan Zhao, John P. Hamilton, Wajid Waheed Bhat, Sean R. Johnson, Grant T. Godden, Taliesin J. Kinser, Benoit Boachon, Natalia Dudareva, Douglas E. Soltis, Pamela S. Soltis, Bjoern Hamberger, C. Robin Buell
Article
Plant Sciences
Mohd Ishfaq Khan, Shahzad A. Pandith, Salika Ramazan, Manzoor A. Shah, Akhtar H. Malik, Zafar A. Reshi
Review
Plant Sciences
Shahzad A. Pandith, Salika Ramazan, Mohd Ishfaq Khan, Zafar A. Reshi, Manzoor A. Shah
Article
Plant Sciences
Niha Dhar, Sreelatha Sarangapani, Vaishnavi Amarr Reddy, Nadimuthu Kumar, Deepa Panicker, Jingjing Jin, Nam-Hai Chua, Rajani Sarojam
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Genetics & Heredity
Kin H. Lau, Wajid Waheed Bhat, John P. Hamilton, Joshua C. Wood, Brieanne Vaillancourt, Krystle Wiegert-Rininger, Linsey Newton, Britta Hamberger, Daniel Holmes, Bjoern Hamberger, C. Robin Buell
Article
Plant Sciences
Garret P. Miller, Wajid Waheed Bhat, Emily R. Lanier, Sean R. Johnson, Davis T. Mathieu, Bjorn Hamberger
Article
Biology
John P. Hamilton, Grant T. Godden, Emily Lanier, Wajid Waheed Bhat, Taliesin J. Kinser, Brieanne Vaillancourt, Haiyan Wang, Joshua C. Wood, Jiming Jiang, Pamela S. Soltis, Douglas E. Soltis, Bjoern Hamberger, C. Robin Buell
Article
Plant Sciences
Mohd Ishfaq Khan, Anwar Shahzad, Irfan Bashir Ganie, Shahzad A. Pandith
Summary: A highly efficient in vitro regeneration system was developed for Rheum spiciforme Royle, resulting in significant increase in seed germination rates with the use of plant hormones in the medium. Different explants showed varying responses to regeneration conditions, leading to successful cultivation and transplantation.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Chemistry, Multidisciplinary
Mervin Chun-Yi Ang, Niha Dhar, Duc Thinh Khong, Tedrick Thomas Salim Lew, Minkyung Park, Sreelatha Sarangapani, Jianqiao Cui, Aniket Dehadrai, Gajendra Pratap Singh, Mary B. Chan-Park, Rajani Sarojam, Michael Strano
Summary: This study developed real-time and nondestructive nanosensors for detecting NAA and 2,4-D in plants, replacing traditional destructive measurement methods. These selective sensors for synthetic auxins showed accumulation capabilities in various plant species, aiding in rapid testing of herbicide susceptibility.
Article
Biochemistry & Molecular Biology
Fani Ntana, Wajid W. Bhat, Sean R. Johnson, Hans J. L. Jorgensen, David B. Collinge, Birgit Jensen, Bjorn Hamberger
Summary: The study found that the expression of the SiTPS gene in Serendipita indica was upregulated when colonizing tomato roots, with its product viridiflorol possessing antifungal properties. While overexpression of SiTPS did not improve fungal colonization ability, viridiflorol inhibited spore germination of a phytopathogenic fungus, indicating that SiTPS and its products may provide a competitive advantage for S. indica during root colonization.
Article
Biotechnology & Applied Microbiology
Shahzad A. Pandith, Niha Dhar, Sumedha Bhosale, Vitthal T. Barvkar, Sumeer Razdan, Manzoor A. Shah, Surrinder K. Lattoo
Summary: Rheum australe, an endangered medicinal and vegetable herb, contains bio-active phyto-constituents with remedying properties. In this study, the RaALS gene, involved in the biosynthesis of these phyto-constituents, was isolated and characterized. The expression of RaALS was found to be correlated with the concentration of metabolites in different parts of the plant. Moreover, RaALS was also responsive to abiotic stressors. These findings highlight the importance of RaALS in the metabolic engineering of R. australe.
PLANT BIOTECHNOLOGY REPORTS
(2023)
Article
Microbiology
Mohd Ishfaq Khan, Nargis Bashir, Shahzad A. A. Pandith, Anwar Shahzad, Vitthal T. T. Barvkar, Anupama A. A. Pable, Manzoor A. A. Shah, Zafar A. A. Reshi
Summary: Two filamentous endophytic ascomycete fungi with different phenotypes, Fusarium proliferatum and Aureobasidium pullulans, were isolated and characterized from the vegetative tissue of the threatened and endemic trans-Himalayan medicinal herb. Molecular identification and chemical analysis showed that these endophytes possibly play an important role in metabolite synthesis of the host.
Review
Plant Sciences
Mohd Ishfaq Khan, Shahzad A. Pandith, Manzoor A. Shah, Zafar A. Reshi
Summary: From simple algal forms to the most advanced angiosperms, calcium oxalate (CaOx) crystals (CRs) are found in majority of photosynthetic organisms. Various studies have shown that CaOx biomineralization is genetically regulated and not a random event. They have roles in calcium regulation, detoxification, light reflectance, protection against grazing, and other functions, making it important to understand their synthesis and regulation in relation to potential crop improvement and climate change. This review critically addresses the origin, synthesis, significance, regulation, and fate of CaOx CRs in plants and soil, presenting them as potential biominerals for future climate-driven issues.
PLANT AND CELL PHYSIOLOGY
(2023)