Review
Microbiology
Bin Hu, Muhammad Junaid Rao, Xiuxin Deng, Sheo Shankar Pandey, Connor Hendrich, Fang Ding, Nian Wang, Qiang Xu
Summary: Citrus Huanglongbing (HLB), also known as citrus greening, is a devastating citrus disease with no commercial citrus cultivars resistant to it. The most prevalent strain associated with HLB, Candidatus Liberibacter asiaticus (CLas), has yet to be cultured in vitro. Research has focused on the complex pathosystem of Ca. Liberibacter, including the interaction of host and CLas, and the impact of CLas infection on host transcripts, proteins, and metabolism. Identifying candidate genes for CLas pathogenicity and improving HLB tolerance in citrus are key research areas for the future.
Article
Plant Sciences
Subhas Hajeri, Sandra Olkowski, Lucita Kumagai, Neil Mcroberts, Raymond K. Yokomi
Summary: Early detection and prompt response are crucial in eradicating 'huanglongbing' in California. A study found that testing the peduncle tissue of fruit consistently resulted in higher detection rates of the disease compared to other tissue types.
Article
Microbiology
Ling Zhang, Ziyi Li, Minli Bao, Tao Li, Fang Fang, Yongqin Zheng, Yaoxin Liu, Meirong Xu, Jianchi Chen, Xiaoling Deng, Zheng Zheng
Summary: CLasMV1, a novel single-stranded DNA phage, was identified in a CLas strain from China. It shares similarities with phages in the Microviridae family. Partial integration of CLasMV1 was found in the chromosome of strain GDHZ11, and complete sequences were found in other CLas strains with variations. PCR tests detected CLasMV1 in a majority of CLas strains from southern China. This discovery expands our understanding of phage diversity in CLas and aids current research in HLB.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Nik J. Cunniffe, Robert Turgeon, Silvio A. Lopes
Summary: The study found that the movement speed of Las in plants is between 2.9-3.8 cm per day, and the colonization of plants by Las is influenced by temperature, with 25.7 degrees Celsius being the optimal condition. This explains the higher impact and incidence of huanglongbing disease during the winter months or regions of milder climates in Brazil.
Article
Agronomy
Chuanyu Yang, Veronica Ancona
Summary: Huanglongbing (HLB) is an important citrus disease caused by an uncultured bacterium called 'Candidatus Liberibacter asiaticus' (CLas). In this study, a method using a dye called propidium monoazide (PMA) was developed to rapidly evaluate the effectiveness of HLB therapies in field experiments. The PMA-qPCR method was able to differentiate between live and dead bacteria, and its effectiveness depended on the primer set used. Application of this method in field tests showed that certain antibiotics displayed significant antibacterial activity against CLas, which was not detected by traditional qPCR analysis. This method could facilitate the validation of treatments against HLB and the implementation of effective management strategies.
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Vikram Dalal, Brajesh Kumar Savita, Poonam Dhankhar, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
Summary: The study utilized structure-based drug design approach to screen and identify potent molecules against 2Cys Prx, which showed higher binding affinity with Prx compared to its substrate. Molecular dynamics analysis revealed that the Prx-inhibitor complexes were more stable.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Plant Sciences
P. A. Silva, J. Huang, N. A. Wulff, Z. Zheng, R. Krugner, J. Chen
Summary: This study presents the draft whole-genome sequence of 'Ca. L. asiaticus' strain 9PA from a sweet orange tree in Sao Paulo State, Brazil, providing valuable genetic resources for research and control of HLB.
Review
Microbiology
Chuanyu Yang, Veronica Ancona
Summary: This review summarizes the main aspects of mechanisms against citrus greening, including inhibition of virulence genes, activation of plant defense genes and metabolites, and the role of citrus microbiome in combatting the disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Yongqin Zheng, Jun Guo, Xiaoling Deng, Zheng Zheng
Summary: The draft genome sequence of a 'Candidatus Liberibacter asiaticus' strain isolated from an HLB-affected lime tree in Myanmar was reported, enriching the current genome database and facilitating research on HLB epidemiology in Asia and beyond.
Article
Plant Sciences
Hongwei Shi, Zuhui Yang, Jie Huang, Haodi Wu, Shimin Fu, Weimin Li, Xiuping Zou, Changyong Zhou, Xuefeng Wang
Summary: Autophagy is manipulated by the citrus Huanglongbing-associated intracellular bacterium CLas. The CLas effector SDE4405 interacts with host ATG8 proteins to activate autophagy and suppress plant defense responses. This study provides new insights into the interaction between CLas and citrus hosts.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jessica Dominguez, Krishnaswamy Jayachandran, Ed Stover, Joseph Krystel, Kateel G. Shetty
Summary: Huanglongbing (HLB), or citrus greening, is a bacterial disease that poses a threat to the sustainability of the citrus industry worldwide. There is no known cure for HLB, but research has found that certain bacteria within citrus trees have the potential to effectively control the disease and may serve as a source of novel antimicrobials.
Article
Plant Sciences
Fengnian Wu, Lihui Liu, Eduardo G. P. Fox, Xiaoling Deng, Meirong Xu, Zheng Zheng, Xiuhong Li, Jiawei Fu, Hui Zhu, Jianjian Huang, Tiejun Deng
Summary: This study investigated the effects of 'Ca. L. asiaticus' infection on key physiological variables in citrus plants. Results showed that most variables, except soluble sugar, were affected by increased infection titers, with the variety C. maxima showing greater resistance. The infection was linked to a decrease in chlorophyll concentration and cell membrane integrity, and an increase in free proline and starch contents.
Article
Agriculture, Multidisciplinary
Honghong Deng, Yi Zhang, Laura Reuss, Joon Hyuk Suh, Qibin Yu, Guolu Liang, Yu Wang, Fred G. Gmitter
Summary: This study compared the volatile changes in HLB-tolerant Sugar Belle and HLB-sensitive Murcott mandarins after CaLas infection, uncovering differences in response to HLB and underlying tolerance mechanisms. Both cultivars showed increased total volatiles and de novo-synthesized new volatiles as key responses to HLB. Additionally, changes in terpenoid biosynthetic pathway, green leaf volatile, and thymol metabolic pathways were observed in response to CaLas infection, with SB mandarins exhibiting simultaneous defense and growth leading to greater HLB tolerance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agronomy
Thais Magni Cavichioli, Maiara Curtolo, Mariangela Cristofani-Yaly, Josiane Rodrigues, Helvecio Della Coletta-Filho
Summary: This study found resistance against Huanglongbing (HLB) in certain citrus hybrid varieties. Long-term observations showed a decrease in infection rate for several hybrids, which also had lower levels of starch accumulation and callose deposition.
Article
Plant Sciences
Qing Zhang, Xiuting Hua, Hong Liu, Yuan Yuan, Yan Shi, Zhengchao Wang, Muqing Zhang, Ray Ming, Jisen Zhang
Summary: The study identified 105 ST genes in Saccharum and revealed different expression patterns, providing insights into the molecular mechanisms of sugar metabolism.
Article
Microbiology
Yue Liu, Huichun Yang, Qi Liu, Xiaowen Zhao, Sasa Xie, Ziting Wang, Ronghui Wen, Muqing Zhang, Baoshan Chen
Summary: The intercropping of soybean and sugarcane is important for sustainable development, with different sugarcane varieties playing a significant role in influencing rhizosphere bacterial communities and nutrient utilization. Specifically, ZZ9 variety was found to increase bacterial diversity and create a more conducive rhizosphere environment for sugarcane growth when intercropped with soybean.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Abdullah Khan, Hongtao Jiang, Junyao Bu, Muhammad Adnan, Syeda Wajeeha Gillani, Muqing Zhang
Summary: This study evaluated the impact of two different sugarcane cultivars on the rhizosphere microbial community and cane morphological characters during consecutive ratooning. The results showed significant changes in the rhizosphere microbiome, with cultivar ZZ-13 causing more alterations and resulting in a more favorable environment for sugarcane growth. This finding contributes to a better understanding of the interaction between rhizosphere microorganisms and ratoon sugarcane.
Article
Biochemistry & Molecular Biology
Sehrish Akbar, Wei Yao, Lifang Qin, Yuan Yuan, Charles A. Powell, Baoshan Chen, Muqing Zhang
Summary: Through transcriptome and proteomics analysis, this study revealed that the Badila sugarcane genotype showed significant upregulation of sugar metabolizing and transporting transcripts after infection with Sugarcane mosaic virus (SCMV), potentially enhancing virus multiplication and infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Zhe Chai, Jinlan Fang, Wei Yao, Yang Zhao, Guangyuan Cheng, Sehrish Akbar, Muhammad Tahir Khan, Baoshan Chen, Muqing Zhang
Summary: ScGAIL is involved in the regulation of plant growth and development, inhibiting sugarcane growth by disrupting the GA signaling pathway.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Jihua Wang, Yan Gu, Yu Mei, Shiqiang Xu, Guoliang Li, Muqing Zhang, Shaohai Yang
Summary: The study aims to estimate the balanced nutrient requirements of sugarcane in China through the QUEFTS model, showing a linear increase in cane yield before reaching 60%-70% of potential yield. The total plants required 1.70 kg N, 0.21 kg P, and 2.52 kg K to produce 1000 kg of cane, with corresponding internal efficiencies of 587.34, 4739.47, and 396.12 kg/kg for N, P, and K.
JOURNAL OF PLANT NUTRITION
(2022)
Article
Plant Sciences
Fan Yu, Xinwang Zhao, Jin Chai, Xueer Ding, Xueting Li, Yongji Huang, Xianhong Wang, Jiayun Wu, Muqing Zhang, Qinghui Yang, Zuhu Deng, Jiming Jiang
Summary: Karyotypes provide important cytogenetic information for understanding phylogenetic relationships and evolutionary origins in related eukaryotic species. Oligonucleotide-based chromosome painting has emerged as a powerful tool for cytogenetic studies, especially in plant species with a large number of chromosomes. By developing oligo-based chromosome painting probes for Saccharum officinarum and conducting comparative chromosome analysis among different species within the Saccharum complex, novel cytotypes and chromosome rearrangements were discovered, shedding light on the evolution of these species.
Article
Microbiology
Zhen Huang, Huixue Li, Yuming Zhou, Yixue Bao, Zhenzhen Duan, Caixia Wang, Charles A. Powell, Baoshan Chen, Muqing Zhang, Wei Yao
Summary: In this study, 316 candidate secreted effector proteins (CSEPs) in the fungus F. sacchari were identified using bioinformatic prediction approaches. The functions of 163 CSEPs were investigated using a heterogeneous expression system in N. benthamiana. We found that some CSEPs suppressed cell death while others caused cell death. Expression analysis of these CSEPs during F. sacchari infection suggested their involvement in sugarcane-F. sacchari interactions.
Article
Agriculture, Multidisciplinary
Abdullah Khan, Ziting Wang, Zhengxia Chen, Junyao Bu, Muhammad Adnan, Muqing Zhang
Summary: The study revealed significant differences in rhizosphere microbiome, soil properties, root structure, and sugar content among 18 sugarcane genotypes under the same environmental condition. Certain genotypes, such as FN-1702, GUC-3, ZZ-13, ZZ-10, and ZZ-6, showed superior performance in terms of bacterial diversity, root structure, soil parameters, and sucrose content.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2021)
Article
Agronomy
Gui Zhi Ling, Jing Lin Xiao, Shu Yang, Dong Ling Li, Xin Lian Tang, Xiao Xia Wang, Mu Qing Zhang, Xiao Feng Li
Summary: This study elucidates the mechanisms by which ammonium alleviates manganese toxicity in sugarcane, including reducing root pectin accumulation and cell wall PME activity, increasing cell wall PMD, and decreasing manganese accumulation. Additionally, nitric oxide mediates the accumulation of pectin and manganese under different nitrogen sources.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2022)
Article
Chemistry, Analytical
Xiuhua Li, Yuxuan Ba, Muqing Zhang, Mengling Nong, Ce Yang, Shimin Zhang
Summary: This study used drone multispectral images to predict canopy nitrogen concentration and irrigation levels for sugarcane, and established corresponding prediction models. The results demonstrated that high resolution multispectral images can effectively provide relevant information for sugarcane crop.
Article
Environmental Sciences
Shimin Zhang, Xiuhua Li, Yuxuan Ba, Xuegang Lyu, Muqing Zhang, Minzan Li
Summary: This study aimed to detect and classify BFW-infected banana canopies using multispectral images and different classification methods, providing recommendations for banana plantation management and approaches for plant disease detection.
Article
Microbiology
Abdullah Khan, Hongtao Jiang, Junyao Bu, Muhammad Adnan, Syeda Wajeeha Gillani, Muhammad Azhar Hussain, Muqing Zhang
Summary: Minimizing the use of chemical fertilizers and determining an appropriate ecofriendly level of nitrogen fertilizer are crucial for sustainable agriculture. This study evaluated the effects of different nitrogen levels on the rhizosphere bacterial composition of sugarcane in the Guangxi region. The results showed that medium-level nitrogen application significantly altered the bacterial community, soil properties, and sugarcane yield. Acidobacteria and Proteobacteria were important phyla in the medium-level treatment. Environmental factors such as pH, available phosphorus and potassium, and soil catalase were found to be key factors influencing the sugarcane rhizosphere microbiome composition.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Chuanyu Yang, Veronica Ancona
Summary: This review summarizes the main aspects of mechanisms against citrus greening, including inhibition of virulence genes, activation of plant defense genes and metabolites, and the role of citrus microbiome in combatting the disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agronomy
Xueting Li, Yirong Guo, Fei Huang, Qiusong Wang, Jin Chai, Fan Yu, Jiayun Wu, Muqing Zhang, Zuhu Deng
Summary: This study used ARMS PCR, chromosome counting, and GISH technology to identify the authenticity of sugarcane and wild sugarcane. The analysis revealed the presence of genuine sugarcane and wild sugarcane materials, which is of great significance for sugarcane breeding.