Article
Horticulture
Margarita Perez-Jimenez, Alfonso Guevara-Gazquez, Antonio Carrillo-Navarro, Jose Cos-Terrer
Summary: The study found that the seed coat of peach seeds had no effect on in vitro germination, while the growth of seeds in media with different concentrations of glucose and sucrose showed significant differences. The root development of seeds was promoted in media with 15 or 30 g.L-1 of sucrose concentration.
Article
Food Science & Technology
Dorine Duijsens, Sarah H. E. Verkempinck, Audrey De Coster, Katharina Paelchen, Marc Hendrickx, Tara Grauwet
Summary: The cooking time of lentils has a significant impact on their microstructure and digestion functionality. Lentils cooked for 15 minutes have slower starch digestion, those cooked for 60 minutes have digestion kinetics similar to cotyledon cells, while those cooked for 30 minutes are in between.
Article
Plant Sciences
Ahmed M. Abdelghaffar, Said. S. Soliman, Tarek A. Ismail, Ahmed M. Alzohairy, Arafat Abdel Hamed Abdel Latef, Khadiga Alharbi, Jameel M. Al-Khayri, Nada Ibrahim M. Aljuwayzi, Diaa Abd El-Moneim, Abdallah. A. Hassanin
Summary: Immature female inflorescences can be used as explants for successful date palm micropropagation, and the choice of culture media is important for different stages and varieties. The concentrations and types of growth regulators also play a crucial role in the success of micropropagation.
Article
Biochemistry & Molecular Biology
Ji Hye Kim, Shucheng Duan, You Rang Park, Seok Hyun Eom
Summary: Thermal processing has an impact on the beneficial antioxidants in lentil seeds. Germinated seeds tend to have lower levels of total phenolic content, major flavonoid content, and antioxidant activity compared to non-germinated seeds, except for one cultivar without a seed coat. The reduction of antioxidants in the seed coat contributes to the decrease in antioxidant activity in germinated seeds of certain cultivars.
Article
Chemistry, Multidisciplinary
Paolo Raffa, Maria Easler, Francesca Cecchinato, Beatrice Auletta, Valentina Scattolini, Silvia Perin, Mattia Francesco Maria Gerli, Paola Caccin, Nicola Elvassore, Paolo De Coppi, Anna Urciuolo
Summary: The study successfully developed a 3D model of neuromuscular tissue using decellularized skeletal muscle constructs, which demonstrated functional contraction and maintained viability of multiple cell types. Additionally, a customized culture system was developed to facilitate easy access, imaging, and analysis of the in vitro engineered co-cultures.
APPLIED SCIENCES-BASEL
(2021)
Article
Horticulture
Li Xu, Fangyun Cheng, Yuan Zhong
Summary: This study presents a protocol for in vitro immature embryo culture in Paeonia ostii 'Feng Dan', which successfully achieved seed germination, rooting, and acclimatization through two steps. The protocol provides a feasible reference for embryo rescue and propagation of tree peony and helps shorten the breeding cycle.
Article
Obstetrics & Gynecology
Evelyn E. Telfer, Claus Yding Andersen
Summary: The cryopreservation of ovarian tissue is widely used to preserve fertility in high-risk females. Besides transplantation, in vitro maturation of oocytes from ovarian tissue can also be used to restore fertility safely in cases where transplantation is contraindicated.
FERTILITY AND STERILITY
(2021)
Article
Multidisciplinary Sciences
Takafumi Matsumura, Kumiko Katagiri, Tatsuma Yao, Yu Ishikawa-Yamauchi, Shino Nagata, Kiyoshi Hashimoto, Takuya Sato, Hiroshi Kimura, Takashi Shinohara, Makoto Sanbo, Masumi Hirabayashi, Takehiko Ogawa
Summary: More than a decade ago, a method using mouse testicular tissue to produce fertile sperm in vitro was established. Although this method has not been effective in other animal species, we recently improved the culture condition to induce spermatogenesis of rats. By introducing acrosin-EGFP transgenic rats and analyzing the metabolomics of the culture media, we successfully induced rat spermatogenesis up to haploid cell production, leading to the production of healthy and fertile offspring through microinsemination. This is the first demonstration of functional haploid cell production and offspring generation in animals other than mice, contributing to the development of an in vitro spermatogenesis system applicable to various mammals.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Applied
Siyuan Liu, Yikai Ren, Hanyue Yin, Michael Nickerson, Mark Pickard, Yongfeng Ai
Summary: The present study found that infrared treatments can reduce the resistant starch content and increase the protein digestibility and soluble dietary fiber content of lentil flours. However, it also decreases the levels of heat-labile B vitamins. These findings are important for the agri-food industry to improve the nutritional profiles of lentil and other flours using infrared processing.
Article
Reproductive Biology
Rob Simmons, Des AR. Tutt, Gizem Guven-Ates, Wing Yee Kwong, R. Labrecque, Federico Randi, Kevin D. Sinclair
Summary: The production of cattle embryos in vitro requires maturation of oocytes in vitro before fertilization. Progesterone support during ovarian stimulation can improve the success of in vitro production. This study investigated the effects of increased peripheral progesterone concentrations during ovarian stimulation cycles.
Article
Plant Sciences
Angela Carra, Caterina Catalano, Ranjith Pathirana, Maurizio Sajeva, Paolo Inglese, Antonio Motisi, Francesco Carimi
Summary: O. ficus-indica is a significant source of forage and food in arid and semiarid ecosystems, and it is widely cultivated worldwide. The study investigated the potential use of in ovulo embryo rescue to increase the proportion of zygotic seedlings in four Italian cultivars. Results showed that embryo culture at 35 post-anthesis days produced the highest percentage of zygotic seedlings. This research provides a pathway to enhance the availability of zygotic seedlings in O. ficus-indica breeding programs.
Article
Multidisciplinary Sciences
Alan Gasinski, Joanna Kawa-Rygielska
Summary: Malting technique can improve the technological parameters, taste, and aroma of legume food products. The malting process significantly influences the volatile composition of lentil seeds, leading to changes in the contribution of different compounds to the overall volatilome.
Article
Multidisciplinary Sciences
Tatsuya Kobayashi, Hiroshi Ishikawa, Kumiko Ishii, Asuka Sato, Natsuko Nakamura, Yoshiko Saito, Hisataka Hasegawa, Maki Fujita, Akira Mitsuhashi, Makio Shozu
Summary: The incidence of 0PN-derived embryos decreased more significantly through time-lapse monitoring (TLM) compared to fixed-point observation. Embryos with early pronucleus (PN) fading formed good-quality blastocysts, and their pregnancy outcomes were similar to those of other embryos.Missed observation of PN appearance in early-cleaved embryos in fixed-point assessment may lead to the formation of 0PN-derived embryos.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Yan Shui, Ming Zhan, Jiajia Li, Shengyan Su
Summary: The present study assessed general strategies for the isolation and in vitro culture of immature ovary-derived cells (oocytes) in red swamp crayfish (Procambarus clarkii). The effects of different digestive enzymes on cell retrieval were examined to facilitate efficient oocyte isolation. Furthermore, the study demonstrated that the exogenous hormone 17-OHP could induce oocyte maturation in vitro.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Sajitha Biju, Sigfredo Fuentes, Claudia Gonzalez Viejo, Damir D. Torrico, Sumayya Inayat, Dorin Gupta
Summary: The study found that lentil seeds grown in an Si-supplied drought-stressed environment showed significant increases in nutritional and antioxidant content, as well as significant reductions in antinutrients. These seeds also received higher acceptability scores among consumers, indicating that Si supplementation can enhance the nutritional and sensory properties of seeds.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Review
Biotechnology & Applied Microbiology
Ileana Gatti, Fernanda Guindon, Carolina Bermejo, Andrea Esposito, Enrique Cointry
PLANT CELL TISSUE AND ORGAN CULTURE
(2016)
Article
Biochemistry & Molecular Biology
I. Gatti, M. A. Esposito, P. Almiron, V. P. Cravero, E. L. Cointry
GENETICS AND MOLECULAR RESEARCH
(2011)
Article
Biochemistry & Molecular Biology
M. A. Esposito, P. Almiron, I. Gatti, V. P. Cravero, F. S. L. Anido, E. L. Cointry
GENETICS AND MOLECULAR RESEARCH
(2012)
Article
Horticulture
Maria Andrea Esposito, Carolina Bermejo, Ileana Gatti, Maria Fernanda Guindon, Vanina Cravero, Enrique Luis Cointry
SCIENTIA HORTICULTURAE
(2014)
Article
Agronomy
Esposito, Maria Andrea, Gatti, Ileana, Cointry, Enrique
Article
Biotechnology & Applied Microbiology
Carolina Bermejo, Maria Fernanda Guindon, Laura Tatiana Palacios, Federico Cazzola, Ileana Gatti, Enrique Cointry
PLANT CELL TISSUE AND ORGAN CULTURE
(2020)
Review
Agriculture, Multidisciplinary
Maria Fernanda Guindon, Federico Cazzola, Tatiana Palacios, Ileana Gatti, Carolina Bermejo, Enrique Cointry
Summary: Biofortification is an approach to increase micronutrient concentrations in edible parts of plants, with various breeding methods available. Peas, recognized for their nutritional value, have untapped micronutrient potential that needs to be considered in development, ensuring nutrient retention post processing and cooking for improved agricultural productivity.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Agriculture, Multidisciplinary
Maria Fernanda Guindon, Maria Gabriela Aguero, Ileana Gatti, Enrique Cointry
Summary: The study of 16 promising pea genotypes revealed that yellow varieties showed superior values in weight and size, while wrinkled varieties performed poorly in terms of moisture content and protein content. By examining protein composition and conducting accelerated aging experiments, varieties suitable for the food industry and highly tolerant to storage could be identified, providing potential for industry exploitation or quality breeding programs.
REVISTA CORPOICA-CIENCIA Y TECNOLOGIA AGROPECUARIA
(2021)
Review
Agriculture, Multidisciplinary
Federico Cazzola, Carolina Julieta Bermejo, Ileana Gatti, Enrique Cointry
Summary: Traditional pulse breeding programs are time consuming, but speed breeding technology can improve efficiency and reduce costs by accelerating photosynthesis and flowering through optimizing light, temperature, etc., without involving transgenesis or gene editing.
CROP & PASTURE SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Maria Andrea Esposito, Ileana Gatti, Carolina Julieta Bermejo, Enrique Luis Cointry
REVISTA DE LA FACULTAD DE CIENCIAS AGRARIAS
(2020)
Article
Agriculture, Multidisciplinary
Carolina Bermejo, Gustavo Rodriguez, Ileana Gatti, Enrique Cointry
Article
Agronomy
Romina M. Cattaneo, Ileana Gatti, Enrique L. Cointry
CIENCIAS AGRONOMICAS
(2015)
Article
Agriculture, Multidisciplinary
I Gatti, VP Cravero, FSL Anido, EL Cointry
PESQUISA AGROPECUARIA BRASILEIRA
(2000)
Article
Biochemistry & Molecular Biology
Ileana Gatti, Fernando Lopez Anido, Vanina Cravero, Pablo Asprelli, Enrique Cointry
GENETICS AND MOLECULAR RESEARCH
(2005)