期刊
PLANT CELL REPORTS
卷 35, 期 6, 页码 1309-1319出版社
SPRINGER
DOI: 10.1007/s00299-016-1963-5
关键词
Cyanovirin-N; Rice crude extract; Endosperm; Anti-HIV; Microbicide
资金
- Ministerio de Ciencia e Innovacion, Spain [BIO2012-35359]
- Center CONSOLIDER on Agrigenomics - MICINN, Spain
- COST Action [FA0804]
- National Cancer Institute, National Institutes of Health [HHSN26120080001E]
- Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research
- ICREA Funding Source: Custom
Cyanovirin-N produced in rice endosperm provides efficient pre-exposure prophylaxis against HIV-1 (BaL) infection in vitro. Cyanovirin-N (CV-N) is a lectin with potent antiviral activity that has been proposed as a component of microbicides for the prevention of infection with Human immunodeficiency virus (HIV). The production of protein-based microbicide components requires a platform that is sufficiently economical and scalable to meet the demands of the large at-risk population, particularly in resource poor developing countries. We, therefore, expressed CV-N in rice endosperm, because the dried seed is ideal for storage and transport and crude extracts could be prepared locally and used as a microbicide component without further purification. We found that crude extracts from rice seeds expressing up to 10 A mu g CV-N per gram dry seed weight showed dose-dependent gp120 binding activity, confirming that the protein was soluble, correctly folded and active. The recombinant lectin ((CV)-C-OS-N) reduced the infectivity of HIV-1(BaL) (an R5 virus strain representing the majority of transmitted infections) by similar to 90 % but showed only weak neutralization activity against HIV-1(RF) (representative of X4 virus, rarely associated with transmission), suggesting it would be highly effective for pre-exposure prophylaxis against the vast majority of transmitted strains. Crude extracts expressing (CV)-C-OS-N showed no toxicity towards human cells at working dilutions indicating that microbicide components produced in rice endosperm are safe for direct application as topical microbicides in humans.
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