Article
Plant Sciences
Shengnan Liu, Yunlu Shi, Fang Liu, Yan Guo, Minhui Lu
Summary: This study presents an optimized transformation system that improves the infection and genetic transformation frequency of immature maize embryos mediated by Agrobacterium tumefaciens. The use of LaCl3 pretreatment enhances the infection frequency, increases the proportion of positive callus, yields more positive regenerated plantlets, and significantly increases the transformation frequency from 8.40% to 17.60%.
PLANT CELL REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Dave Peterson, Pierluigi Barone, Brian Lenderts, Chris Schwartz, Lanie Feigenbutz, Grace St Clair, Spencer Jones, Sergei Svitashev
Summary: CRISPR-Cas is a powerful tool for DNA double-strand break technology, with wide applications in plant genome modification. Advancements in maize transformation and vector design have greatly increased the efficiency of targeted gene insertion, allowing for potential trait improvement in a wide range of crop species.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Xudong Ye, Zarir Vaghchhipawala, Edward J. Williams, Changlin Fu, Jinyuan Liu, Fengming Lu, Erin L. Hall, Shirley X. Guo, LaRee Frank, Larry A. Gilbertson
Summary: Efficient selectable marker gene autoexcision in transgenic plants of soybean, cotton, canola, and maize is achieved by effective Cre recombinase expression. Different crops require diverse promoters to drive Cre expression, and the efficiency of marker-free progeny generation varies among different crops.
PLANT CELL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Ina Becker, Binod Prasad, Maria Ntefidou, Viktor Daiker, Peter Richter, Michael Lebert
Summary: Euglena gracilis demonstrates high sensitivity to hygromycin and zeocin, offering potential resistance marker genes for transformant selection. Agrobacterium-mediated transformation (ATMT) yielded hygromycin-resistant cells, but GUS-negative cells indicated explicit silencing of the gus gene. Optimization of cocultivation factors led to higher transformant numbers and an efficiency of 8.26 +/- 4.9% under optimized conditions, demonstrating potential for bioengineering E. gracilis for high-value product production and fundamental studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yanping Song, Wenbo Luo, Ying Wu, Xiaojie Li, Nick W. W. Albert, Yinhai Zhang, Xiangtao Chen, Kui Lin-Wang, Cecilia H. H. Deng, Zhubing Hu, Bingsong Zheng, Xiaofei Wang, Richard V. V. Espley, Fuqiang Cui
Summary: This study provides a propagation method and a gene transformation method for bilberry, which will benefit bilberry breeding and bioreactor development.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Min Wang, Yang-Yang Qin, Nan-Nan Wei, Huan-Ying Xue, Wen-Shan Dai
Summary: In this study, a rapid, simple and highly efficient hairy root transformation system was developed for functional analysis of citrus genes. The system enables subcellular localization, gene overexpression and gene editing studies. The results demonstrate the significant value of this technology in citrus gene research.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Horticulture
Shiwei Zhong, Bin Dong, Jie Zhou, Yunfeng Miao, Liyuan Yang, Yiguang Wang, Zhen Xiao, Qiu Fang, Qianqian Wan, Hongbo Zhao
Summary: This study aims to develop an efficient transient expression protocol for sweet osmanthus to quickly identify gene functions. It was found that the optimal protocol for transient expression varied among different tissues, playing a crucial role in improving transformation efficiency.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Kuang-Teng Wang, Ming-Chang Hong, Yu-Sheng Wu, Tsung-Meng Wu
Summary: Duckweed is one of the smallest flowering plants in the world and is used for livestock as biofactors and feedstuff additives due to its high reproduction rate and biomass. This study successfully established a micropropagation technique and Agrobacterium-mediated transformation in duckweed.
Article
Plant Sciences
Stijn Aesaert, Lennert Impens, Griet Coussens, Els Van Lerberge, Rudy Vanderhaeghen, Laurence Desmet, Yasmine Vanhevel, Shari Bossuyt, Angeline Ndele Wambua, Mieke Van Lijsebettens, Dirk Inze, Ellen De Keyser, Thomas B. Jacobs, Mansour Karimi, Laurens Pauwels
Summary: This study made a breakthrough in plant transformation by co-transforming morphogenic transcription factors to induce somatic embryogenesis. It also showed that modifying tissue culture media and using specific constructs and control systems can reduce undesirable phenotypes and increase transformation efficiency and gene editing functionality.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Xiaoyu Zhang, Gencheng Xu, Chaohua Cheng, Lei Lei, Jian Sun, Ying Xu, Canhui Deng, Zhigang Dai, Zemao Yang, Xiaojun Chen, Chan Liu, Qing Tang, Jianguang Su
Summary: This study successfully generated gene-edited plants in Cannabis sativa using an Agrobacterium-mediated transformation method, improving shoot regeneration efficiency and editing the phytoene desaturase gene with CRISPR/Cas9 technology.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemical Research Methods
Jiaying Sun, Jiamei Zhao, Hongming Huang, Jiaqi Jia, Mingyue Yuan, Shuqin Xiao, Chunsheng Xue
Summary: In this study, a gene knockout transformation system was established to explore the molecular mechanisms of fungal virulence to maize. By knocking out specific genes, it was found that the mutant strains caused significantly reduced disease index and lesion number in maize, indicating a decrease in fungal virulence due to the absence of these genes.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Zhelong Lin, Lei Chen, Shanjie Tang, Mengjie Zhao, Tong Li, Jia You, Changqing You, Boshu Li, Qinghua Zhao, Dongmei Zhang, Jianli Wang, Zhongbao Shen, Xianwei Song, Shuaibin Zhang, Xiaofeng Cao
Summary: The lack of genome editing platforms has hindered the study and improvement of forage crops that can be grown on lands not suitable for other crops. This study established an efficient CRISPR/Cas9 genome editing method in a stress-tolerant forage grass called sheepgrass, and knocking out the TB1 gene significantly increased tiller number and biomass.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Patricia L. Polowick, Wei Yan
Summary: A reliable protocol was developed for Agrobacterium-mediated genetic transformation of Lens culinaris Medik with a higher frequency than previous reports, multiple shoots per transgenic event, consistent rooting, minimal escapes, no evidence of chimaeras and confirmed inheritance. The protocol utilized longitudinal slices of embryo axes from imbibed mature seed as a starting explant and a plasmid containing a beta-glucuronidase:neomycin phosphotransferase (gus:nptII) fusion gene in Agrobacterium strain EHA105. Various factors such as the optical density of the Agrobacterium suspension and type and concentration of plant growth regulators were tested. The gus reporter gene expression was confirmed through histochemical staining and molecular analysis.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Kari Johnson, Uyen Cao Chu, Geny Anthony, Emily Wu, Ping Che, Todd J. Jones
Summary: Two wheat transformation methods using morphogenic regulator genes, with or without gene excision, have been developed. These methods achieve independent transformation efficiencies of up to 58% and 75%, respectively, and significantly reduce tissue culture duration. The procedures are simplified, less labor-intensive, higher-throughput, and cost-effective.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Savio D. Rodrigues, Mansour Karimi, Lennert Impens, Els Van Lerberge, Griet Coussens, Stijn Aesaert, Debbie Rombaut, Dominique Holtappels, Heba M. M. Ibrahim, Marc Van Montagu, Jeroen Wagemans, Thomas B. Jacobs, Barbara De Coninck, Laurens Pauwels
Summary: Agrobacterium spp. are important plant pathogens that can be genetically modified using CRISPR-mediated base editing to introduce targeted point mutations, improving the efficiency and functionality of the strains for plant transformation and gene editing. The method developed in this study has shown high efficiency and potential for future advancements in engineering Agrobacterium strains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Plant Sciences
Elohim Bello-Bello, Damar Lopez-Arredondo, Thelma Y. Rico-Chambron, Luis Herrera-Estrella
Summary: Global agriculture and food security are facing significant challenges due to climate change and land degradation, particularly regarding human-induced soil compaction and its negative impacts on soil fertility, root system development, and crop yield. To address this challenging scenario, it is necessary to develop compaction-resilient crops with enhanced root system penetrability through approaches such as root-targeted genetic engineering and genomics-assisted breeding. These approaches have the potential to enhance crop resilience to climate change and marginal soils, as well as promote CO2 sequestration and storage in deeper soil strata.
TRENDS IN PLANT SCIENCE
(2022)
Editorial Material
Plant Sciences
Horacio Esteban Hopp, German Spangenberg, Luis Herrera-Estrella
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jonathan Odilon Ojeda-Rivera, Mauricio Ulloa, Philip A. Roberts, Pratibha Kottapalli, Congli Wang, Hector-Rogelio Najera-Gonzalez, Paxton Payton, Damar Lopez-Arredondo, Luis Herrera-Estrella
Summary: Cotton is an important crop for textile fiber production, but it is threatened by parasitic nematodes such as Meloidogyne incognita. This study explores the molecular mechanisms of resistance to M. incognita in cotton and finds that the constitutive activation of defense-related genes and phytohormone responses, specifically jasmonic and salicylic acid, in the roots of resistant cotton cultivars contribute to RKN resistance. The findings suggest promising candidate genes for introducing RKN resistance into commercial cotton varieties.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ricardo A. Chavez A. Montes, Mauricio Ulloa, Tammy Biniashvili, Arik Zackay, Nir Kfir, Damar Lopez-Arredondo, Luis Herrera-Estrella
Summary: This study reports a high-quality genome assembly and annotation of Pima-S6 cotton and compares it with other Gossypium genome assemblies at the chromosome and protein level. The results reveal important differences in chromosome structure and annotated protein content between Pima-S6 and other G. barbadense assemblies, as well as across Gossypium assemblies in general. Detailed synteny analyses demonstrate chromosomal rearrangements between Pima-S6 and other Pima genomes, raising questions about the true chromosome structure of Gossypium barbadense genomes.
Article
Plant Sciences
Feifei Yi, Aosong Song, Kai Cheng, Jinlei Liu, Chenxiao Wang, Lili Shao, Shuang Wu, Ping Wang, Jiaxuan Zhu, Zhilin Liang, Ying Chang, Zongyan Chu, Chaowei Cai, Xuebin Zhang, Pei Wang, Aimin Chen, Jin Xu, David J. Burritt, Luis Herrera-Estrella, Lam-Son Phan Tran, Weiqiang Li, Yingfan Cai
Summary: Strigolactones enhance cotton resistance to Verticillium wilt by interacting with other hormone signaling pathways and regulating hormone homeostasis through a negative feedback loop. Strigolactone is involved in Verticillium wilt resistance in cotton. The analog rac-GR24 enhances cotton resistance to Verticillium wilt, while the biosynthesis inhibitor TIS108 decreases resistance.
Editorial Material
Plant Sciences
Nidhi Kandhol, Vijay Pratap Singh, Luis Herrera-Estrella, Lam-Son Phan Tran, Durgesh Kumar Tripathi
Summary: Genetic modification enables the alteration of important traits in crops, but it involves costly and laborious procedures to modify the genetic background. Thagun et al. recently introduced a nanocarrier-based foliar spray technique that allows the translocation of bioactive molecules into plant cells without the need for introducing transgenes, thus enabling the engineering of important traits.
TRENDS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Long Wang, Xianqing Jia, Lei Xu, Jiahong Yu, Suna Ren, Yujie Yang, Kaibin Wang, Damar Lopez-Arredondo, Luis Herrera-Estrella, Hans Lambers, Keke Yi
Summary: As a finite and non-renewable resource, phosphorus is crucial for crop growth and food production. However, agricultural use and associated loss of phosphorus to the aquatic environment are causing environmental pollution and threatening global food security. Microalgae-based biological phosphorus recovery is considered a promising solution, but the low accumulation capacity of algae restricts its application. By manipulating genes involved in cellular phosphorus accumulation and signaling, researchers have tripled the phosphorus storage capacity of algae and significantly improved its phosphorus recovery efficiency from wastewater. This genetic engineering approach has the potential to enhance phosphorus uptake and accumulation in microalgae, and accelerate the application of algae for phosphorus recovery and closing the phosphorus cycle.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Editorial Material
Plant Sciences
Nidhi Kandhol, Sangeeta Pandey, Vijay Pratap Singh, Luis Herrera-Estrella, Jose Lopez Bucio, Lam-Son Phan Tran, Durgesh Kumar Tripathi
TRENDS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Valeria Flores Tinoco, Luis Herrera-Estrella, Damar Lopez-Arredondo
Summary: Only two primary photosynthetic endosymbiosis are known to have occurred throughout evolution, resulting in the formation of Archaeplastida and Paulinella spp. The fundamental questions surrounding primary endosymbiosis remain unanswered, but recent advancements in chimeric photosynthetic life-forms offer new opportunities for studying them. Cournoyer et al. have successfully established artificial photosynthetic endosymbiosis between yeast and cyanobacteria.
TRENDS IN PLANT SCIENCE
(2023)
Article
Biology
Gerardo Alejo-Jacuinde, Hector-Rogelio Najera-Gonzalez, Ricardo A. Chavez A. Montes, Cristian D. Gutierrez D. Reyes, Alfonso Carlos Barragan-Rosillo, Benjamin Perez Sanchez, Yehia Mechref, Damar Lopez-Arredondo, Lenin Yong-Villalobos, Luis Herrera-Estrella
Summary: This study presents a chromosome-level reference genome for chia and analyzes the transcriptome, proteome, and metabolome to uncover the molecular basis of the unique characteristics of chia seeds. Chia is an emerging crop with functional food properties, but the genetic basis for traits like seed mucilage and polyphenol content remains unclear.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biology
Alethia A. Brito-Bello, Damar Lopez-Arredondo
Summary: The excessive use of synthetic pesticides has led to environmental problems, human health risks, and the development of resistance in various organisms. Allelochemicals produced by plants and microorganisms are a potential alternative to synthetic pesticides in addressing these problems. Microalgae, in particular, offer new opportunities to discover secondary metabolites with pesticide activities and novel modes of action.
