Article
Plant Sciences
Sakiko Ishida, Hidemasa Suzuki, Aya Iwaki, Shogo Kawamura, Shohei Yamaoka, Mikiko Kojima, Yumiko Takebayashi, Katsushi Yamaguchi, Shuji Shigenobu, Hitoshi Sakakibara, Takayuki Kohchi, Ryuichi Nishihama
Summary: In this study, it was demonstrated that in the liverwort Marchantia polymorpha, the decrease in endogenous auxin level at the cut surface of decapitated explants induced the expression of a key transcription factor gene, MpLAXR. The expression of MpLAXR was able to overcome the inhibition of regeneration by exogenously applied auxin, and ectopic expression of MpLAXR promoted cell proliferation in normally quiescent tissues. These findings provide insights into stem cell regulation and apical dominance establishment in land plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Li Zhang, Tianhong Li, Shengzhong Su, Hao Peng, Sudi Li, Ke Li, Luyao Ji, Yaoyun Xing, Junchuan Zhang, Xinglin Du, Mingdi Bian, Yuying Liao, Zhenming Yang, Zecheng Zuo
Summary: This study found that the ubiquitination of the photo-responsive transcription factor HY5 by the COP1/SPA complex is conserved in the liverwort Marchantia polymorpha, but unlike in Arabidopsis, the blue light receptor CRY does not inhibit this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hidemasa Suzuki, Hirotaka Kato, Megumi Iwano, Ryuichi Nishihama, Takayuki Kohchi
Summary: Auxin perception by TIR1/AFBs receptors regulates gene expression and plays pleiotropic roles in plant development. The essentiality of TIR1-mediated auxin perception for cell viability in Arabidopsis thaliana is uncertain. Here we show that the single TIR1/AFB receptor in the liverwort M. polymorpha, MpTIR1, functions as an evolutionarily conserved auxin receptor. Genetic, biochemical, and transcriptomic analyses reveal that MpTIR1 is involved in the developmental transition and establishment of stem cells during organogenesis in M. polymorpha. Our findings suggest that, in this model plant, auxin signaling is dispensable for cell division but essential for the three-dimensional patterning of the plant body through pluripotent stem cell establishment.
Article
Developmental Biology
Naoki Minamino, Takuya Norizuki, Shoji Mano, Kazuo Ebine, Takashi Ueda
Summary: Gametogenesis is a crucial process in sexual reproduction, and the sperm development in bryophytes involves intricate cellular reorganization. This study classified the developmental processes during sperm formation in the liverwort Marchantia polymorpha based on changes in cellular and nuclear shapes, as well as flagellar development. The researchers also investigated the remodeling of microtubules and the reorganization of endomembrane organelles. The findings revealed changes in the glutamylation of tubulin during flagella and spline formation, and the precise and regulated reorganization of plasma membrane and endomembrane organelles involving the functions of endosomal sorting complexes required for transport (ESCRT) machineries. These findings provide valuable insights for the classification of sperm development in bryophytes.
Article
Biochemistry & Molecular Biology
Rui-Lin Xiong, Jiao-Zhen Zhang, Xin-Yan Liu, Jian-Qun Deng, Ting-Ting Zhu, Rong Ni, Hui Tan, Ju-Zheng Sheng, Hong-Xiang Lou, Ai-Xia Cheng
Summary: Liverworts contain bibenzyls and O-glycosides with antioxidant activity. Two bibenzyl UDP-glucosyltransferases, MpUGT737B1 and MpUGT741A1, were identified from the liverwort Marchantia polymorpha. MpUGT741A1 specifically accepted the bibenzyl lunularin as a substrate, while MpUGT737B1 accepted bibenzyls, dihydrochalcone, and phenylpropanoids as substrates and converted phloretin to phloretin-4-O-glucoside and phloridzin. Both enzymes showed selectivity towards UDP-glucose as a sugar donor. The expression levels of these enzymes increased after UV irradiation, suggesting their role in plant stress adaptation. Subcellular localization revealed their presence in the cytoplasm and nucleus. These enzymes have potential for synthesizing bioactive bibenzyl O-glucosides and enhancing plant antioxidant capacity.
Article
Plant Sciences
Haruka Shinkawa, Masataka Kajikawa, Tomoyuki Furuya, Ryuichi Nishihama, Hirokazu Tsukaya, Takayuki Kohchi, Hideya Fukuzawa
Summary: This study discovered the important role of DYRKs protein kinases, specifically the MpYAK1 subfamily, in regulating plant growth and development in the liverwort Marchantia polymorpha. MpYAK1 was found to be involved in maintaining plant responses to light conditions and nutrient signaling.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Takuya Norizuki, Naoki Minamino, Miyuki Sato, Takashi Ueda
Summary: This study investigates the involvement of autophagy in the degradation and reorganization of plastids during spermiogenesis in the liverwort Marchantia polymorpha. The results show that autophagy-dependent degradation of plastids occurs during spermiogenesis and impaired autophagy leads to defective morphological changes and starch accumulation in the plastid.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Conner M. Lay, Morgan Sindall, Sheldon K. McLetchie, D. Nicholas McLetchie
Summary: Phytochromes are important in allowing plants to sense and react to environmental changes. This research investigated the germination responses of sexual and asexual offspring of a tropical liverwort and found that they are mediated by phytochromes. This suggests that they may germinate and grow in similar microhabitats.
Editorial Material
Cell Biology
Takuya Norizuki, Takashi Ueda
Summary: Sperm mitochondria in bryophytes undergo reorganization through mitochondrial fission and degradation processes. Other cytoplasmic components are also degraded via autophagy, but mitochondria have a distinct degradation time. This study highlights the dynamic reorganization of organelles through multiple degradation pathways during spermiogenesis in M. polymorpha.
Article
Evolutionary Biology
George Sandler, Aneil F. Agrawal, Stephen Wright
Summary: This study examines the genetic diversity of the facultatively sexual liverwort Marchantia polymorpha and finds no evidence of strictly clonal descent or isolation by distance. Instead, evidence of recent recombination and gene flow between geographically distant isolates suggests a modest frequency of sexual reproduction. However, the genome of M. polymorpha has low levels of nucleotide diversity and signs of inefficient selection, indicating possible linked selection and a small effective population size due to asexual propagation. Overall, the genome reveals a complex history of both sexual and asexual reproduction in M. polymorpha.
GENOME BIOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Mateusz Stelmasiewicz, Lukasz Swiatek, Agnieszka Ludwiczuk
Summary: Liverwort endophytes, particularly those from spore-forming plants, could be a valuable source of new biologically active substances not found in other organisms. In this study, volatile compounds obtained from Marchantia polymorpha L. liverwort and its endophytes were analyzed. Diketopiperazine compounds were identified in the endophyte extract, while the liverwort volatile extract contained sesquiterpenoids of cuparane, chamigrane, acorane, and thujopsane types. The ethyl acetate extracts from the endophytic microorganisms showed significant anticancer potential against hypopharyngeal squamous cell carcinoma and cervical adenocarcinoma.
Article
Plant Sciences
Go Takahashi, Shigeyuki Betsuyaku, Natsuki Okuzumi, Tomohiro Kiyosue, Yuki Hirakawa
Summary: Research has shown that peptide hormones MpCLE1 and MpCLE2 in Marchantia polymorpha play crucial roles in cell division and sexual reproduction by interacting with specific receptors at the apical meristem. Compared to other plants, Marchantia polymorpha possesses a minimal set of CLE family peptides, exhibiting higher conservation in evolutionary terms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Shahin Imran, Masumi Oyama, Rie Horie, Natsuko Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie
Summary: This study investigates the structure and function of the high-affinity potassium transporter (MpHKT1) in the liverwort Marchantia polymorpha. The C-terminal hydrophilic domain of MpHKT1 is found to play a unique role in regulating transport activity and ion selectivity. Overexpression of MpHKT1 leads to higher Na+ accumulation, while phenotypes with truncated C-terminal domains are weaker.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Ulf Lagercrantz, Anja Billhardt, Sabine N. Rousku, Martina Leso, Salim Hossain Reza, D. Magnus Eklund
Summary: Previous studies on the evolution of plant circadian clocks have often focused on genes and models defined in Arabidopsis, but this study took a more unbiased approach by looking at clock genes in liverwort Marchantia polymorpha. The results showed that a homologue to the transcriptional co-repressor Arabidopsis DE-ETIOLATED1 plays an important role in clock gene expression and rhythms, particularly in the liverwort's circadian clock.
Article
Plant Sciences
Erika Bellini, Elena Bandoni, Silvia Giardini, Carlo Sorce, Carmelina Spano, Stefania Bottega, Debora Fontanini, Arian Kola, Daniela Valensin, Andrea Bertolini, Alessandro Saba, Luca Paoli, Andrea Andreucci, Mingai Li, Claudio Varotto, Luigi Sanita di Toppi
Summary: Plants have developed mechanisms to control the uptake and accumulation of both essential and non-essential metals. This includes chelation and sequestration of metals by thiol ligands, such as glutathione and phytochelatins. In the liverwort Marchantia polymorpha, the cell wall moderately reduces the influx of cadmium into cells, and intracellularly, cadmium induces the activity of phytochelatin synthase, leading to synthesis of phytochelatins. Glutathione and phytochelatins chelate cadmium at the cytosolic level, allowing its detoxification.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Xiaojuan Zhang, Mika Nomoto, Marta Garcia-Leon, Naoki Takahashi, Mariko Kato, Kei Yura, Masaaki Umeda, Vicente Rubio, Yasuomi Tada, Tsuyoshi Furumoto, Takashi Aoyama, Tomohiko Tsuge
Summary: This study found that the plant CFI 25 subunit plays an important role in maintaining the diversity of mRNA 3' ends. Multiple forms of CFI exist in plants, and AtCFI 25a is essential for maintaining the proper diversity of 3' end lengths of transcripts coding for CFI subunits and plays important roles in Arabidopsis development.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tomoyuki Furuya, Ryuichi Nishihama, Kimitsune Ishizaki, Takayuki Kohchi, Hiroo Fukuda, Yuki Kondo
Summary: Plants maintain continuous development by precisely balancing cell proliferation and differentiation. A study on the liverwort Marchantia polymorpha revealed that MpGSK, a member of the GSK3 kinase family, plays an important role in regulating cell differentiation and proliferation.
PLANT BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Mai Uzaki, Kotaro Yamamoto, Akio Murakami, Yushiro Fuji, Miwa Ohnishi, Kimitsune Ishizaki, Hidehiro Fukaki, Masami Yokota Hirai, Tetsuro Mimura
Summary: This study compared the changes in idioblast cells and laticifer cells during Catharanthus roseus seedling growth, and found that the accumulation of fluorescent metabolites in these cells was differentially regulated. Analysis revealed that the fluorescence observed in these cells was emitted from the compound serpentine. The serpentine content in leaves also increased with leaf growth. These findings suggest that idioblast cells and laticifer cells have different biological roles in MIA biosynthesis and regulation.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Plant Sciences
Shahin Imran, Masumi Oyama, Rie Horie, Natsuko Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie
Summary: This study investigates the structure and function of the high-affinity potassium transporter (MpHKT1) in the liverwort Marchantia polymorpha. The C-terminal hydrophilic domain of MpHKT1 is found to play a unique role in regulating transport activity and ion selectivity. Overexpression of MpHKT1 leads to higher Na+ accumulation, while phenotypes with truncated C-terminal domains are weaker.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yuuki Sakai, Takumi Higaki, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Seiichiro Hasezawa
Summary: This study used live-cell imaging to analyze the microtubule dynamics during the first asymmetric division of the spore in Marchantia polymorpha. The researchers found that the preprophase band was not formed in the spore and that the bipolar prospindle migrated from the center to the periphery, indicating cell polarity. They also discovered that actin filaments played a role in the occurrence of asymmetric division. These findings provide insights into cell-autonomous asymmetric division in plants.
PLANT BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Richalynn Leong, Javier Jingheng Tan, Sally Shuxian Koh, Ting-Ying Wu, Kimitsune Ishizaki, Daisuke Urano
Summary: This study investigated the cellular mechanisms of calcium deficiency in plants using the bryophyte Marchantia polymorpha as a model. The results revealed metabolic compounds and genetic pathways involved in the occurrence and expansion of foliar tipburn. Furthermore, comparable changes in gene expression were observed in other plant species, suggesting the conservation of these pathways.
Article
Biochemistry & Molecular Biology
Hidemasa Suzuki, Hirotaka Kato, Megumi Iwano, Ryuichi Nishihama, Takayuki Kohchi
Summary: Auxin perception by TIR1/AFBs receptors regulates gene expression and plays pleiotropic roles in plant development. The essentiality of TIR1-mediated auxin perception for cell viability in Arabidopsis thaliana is uncertain. Here we show that the single TIR1/AFB receptor in the liverwort M. polymorpha, MpTIR1, functions as an evolutionarily conserved auxin receptor. Genetic, biochemical, and transcriptomic analyses reveal that MpTIR1 is involved in the developmental transition and establishment of stem cells during organogenesis in M. polymorpha. Our findings suggest that, in this model plant, auxin signaling is dispensable for cell division but essential for the three-dimensional patterning of the plant body through pluripotent stem cell establishment.
Article
Plant Sciences
Nobiza Khatun, Akihisa Shinozawa, Kosaku Takahashi, Hideyuki Matsuura, Akida Jahan, Mousona Islam, Masudul Karim, Sk Rahul, Mikako Yoshikawa, Kimitsune Ishizaki, Yoichi Sakata, Daisuke Takezawa
Summary: Low-molecular-weight sugars and the stress hormone abscisic acid (ABA) play important roles in protecting cellular membranes and macromolecules during dehydration. ABA also regulates the positive feedback loop for sugar-induced sugar accumulation in liverworts, enhancing desiccation tolerance.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Aoi Narutaki, Prihardi Kahar, Shunji Shimadzu, Shota Maeda, Tomoyuki Furuya, Kimitsune Ishizaki, Hidehiro Fukaki, Chiaki Ogino, Yuki Kondo
Summary: In this study, the effects of sugars on vascular cell differentiation were examined using a vascular cell induction system. Sucrose was found to have the strongest inhibitory effect on xylem differentiation. Transcriptome analysis revealed that sucrose suppresses xylem and phloem differentiation in cambial cells. The central regulator BRI1-EMS-SUPPRESSOR1 transcription factor was suggested to be involved in the sucrose-mediated regulation of vascular cell differentiation.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Miki Hatada, Ryota Akiyama, Moeko Yamagishi, Kimitsune Ishizaki, Masaharu Mizutani
Summary: This study analyzed the sterol profiles in the bryophyte model plant Marchantia polymorpha L. and identified the presence of sterol biosynthetic genes. Functional analysis revealed the importance of one of these genes for the normal growth and development of the plant.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Chieko Goto, Akira Ikegami, Tatsuaki Goh, Kaisei Maruyama, Hiroyuki Kasahara, Yumiko Takebayashi, Yuji Kamiya, Koichi Toyokura, Yuki Kondo, Kimitsune Ishizaki, Tetsuro Mimura, Hidehiro Fukaki
Summary: In Arabidopsis thaliana, the mutation in the GNOM gene severely inhibits lateral root (LR) formation, while the fewer roots suppressor1 (fsp1) mutation partially restores LR formation. It has been found that fsp1 mutation increases endogenous IAA levels and the number of sites where auxin response accumulates before LR formation, and SUR2 gene affects IAA accumulation in the pericycle cells at the xylem pole.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rui Sun, Maiko Okabe, Sho Miyazaki, Toshiaki Ishida, Kiyoshi Mashiguchi, Keisuke Inoue, Yoshihiro Yoshitake, Shohei Yamaoka, Ryuichi Nishihama, Hiroshi Kawaide, Masatoshi Nakajima, Shinjiro Yamaguchi, Takayuki Kohchi
Summary: In the liverwort Marchantia polymorpha, the synthesis of gibberellin and related genes play important roles in light responses.
Article
Plant Sciences
Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima
Summary: This study analyzed the impact of membrane lipid remodeling on inorganic phosphate uptake and discovered the crucial role of MpPAH in the growth of Marchantia polymorpha. The mutation of MpPAH affects plant growth by suppressing the synthesis of plastid glycolipids through the endoplasmic reticulum pathway.
Article
Plant Sciences
Sayuri Ando, Mika Nomoto, Hidekazu Iwakawa, Simon Vial-Pradel, Lilan Luo, Michiko Sasabe, Iwai Ohbayashi, Kotaro T. Yamamoto, Yasuomi Tada, Munetaka Sugiyama, Yasunori Machida, Shoko Kojima, Chiyoko Machida
Summary: Arabidopsis ASYMMETRIC LEAVES2 (AS2) is crucial for the formation of flat symmetric leaves. AS2 represses the expression of ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3) gene. The formation of AS2 bodies is tightly linked to the morphology and functions of the nucleolus.