期刊
PLANT BREEDING
卷 135, 期 6, 页码 721-727出版社
WILEY-BLACKWELL
DOI: 10.1111/pbr.12427
关键词
male; marker-assisted selection; OGI; persimmon (Diospyros kaki Thunb.); sex determination
资金
- Special Scientific Research Fund of Agricultural Public Welfare Profession of China [201203047]
- Special Fund for National Science & Technology Basic Research [2012 FY110100]
- Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains [2015TD01]
It is crucial to develop a rapid technique for identifying sexuality in the seedling stage of persimmon (Diospyros kaki Thunb.), and the elimination of male progeny has been regarded as an important strategy for enhancing breeding efficiency. In this study, phenotype characterization and genotyping of the male-linked OGI marker were carried out using 205 accessions, including persimmon cultivars, F-1 progeny and nine related Diospyros species. All persimmon cultivars displayed consistent results regarding OGI amplification and sex phenotype. A total of 143 F-1 progeny were derived from 11 crosses, among which 95 individuals had flowered. In the flowering full-sib families, the amplification of the OGI marker in agreement with the sex phenotype was obtained in 85 plants (89.5%). The segregation of OGI in Huashi 1'xLuotian Tianshi' and Huashi 1'xMale 3 F-1 populations fit a 1:1 ratio. Furthermore, high OGI transferability was observed in nine related species. Overall, the results indicated that the OGI locus could be used to distinguish male from female persimmon plants at an early stage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据