Article
Biochemical Research Methods
Josie L. McQuillan, Anthony J. Berndt, Ashley E. Sproles, Stephen P. Mayfield, Jagroop Pandhal
Summary: In this study, novel genetic devices for transgene expression in Chlamydomonas reinhardtii have been developed by identifying cis-regulatory DNA elements capable of driving high transgene expression. This provides a new toolbox of cis-regulatory elements for driving transgene expression at various levels and reveals insights into promoter structure and gene regulation in C. reinhardtii.
Article
Engineering, Environmental
Antonia Schad, Heiko Wagner, Christian Wilhelm
Summary: One of the central goals of algal biotechnology is to replace fossil fuels with autotrophic production of organic matter. Hence, numerous studies are currently focusing on improving the efficiency of algal biotechnology approaches. In this paper, a multidimensional analysis of the growing conditions is used to estimate the energetic and economic parameters of the process control in the production of glycolate using the green alga Chlamydomonas reinhardtii. Under optimized conditions, a high yield of up to 7.5 g glycolate per g biomass can be achieved.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Microbiology
Emma O. Fuentes-Ramirez, Miriam Vazquez-Acevedo, Alfredo Cabrera-Orefice, Sergio Guerrero-Castillo, Diego Gonzalez-Halphen
Summary: This study provides a morphological and proteomic characterization of the colorless alga Polytomella parva, identifying several plastid proteins involved in various metabolic pathways. The results contribute to a better understanding of the metabolism and biological activities of this unicellular, free-living alga.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sibaji K. Sanyal, Komal Sharma, Diksha Bisht, Sunita Sharma, Kumari Sushmita, Suneel Kateriya, Girdhar K. Pandey
Summary: Ca2+ signaling is crucial for perceiving and transmitting information in cells. The Ca2+-mediated CBL-CIPK module is a major sensor responder network for Ca2+ signaling, and its involvement in plant adaptation from aquatic to terrestrial habitats has been suggested. Experimental analysis showed that CrCBL1 protein interacts with Phototropin and Channelrhodopsin, and its expression is regulated by light. Comparing algal sequences, differences in CBL and CIPK proteins between aquatic and terrestrial plants were identified. Additionally, Klebsormidium CBL and CIPK genes were found to respond to abiotic stress, indicating their early role in terrestrial adaptations. Structure-based prediction and Ca2+-binding assays revealed a typical Ca2+-binding pocket in Klebsormidium's KnCBL1 protein. Overall, this study suggests that stress-responsive proteins facilitate crosstalk between Ca2+ and light signaling pathways during plant adaptation from aquatic to terrestrial habitats.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Jiale Qiu, Jie Zhang, Huihui Zhao, Cuiping Wu, Caoliang Jin, Xiangdong Hu, Jian Li, Xiuling Cao, Shenkui Liu, Xuejiao Jin
Summary: Carbonate stress has significant impacts on agriculture and industry, but the role of cell wall-related genes in resistance to carbonate remains poorly understood. This study reveals the critical role of cellulose in the NaHCO3 resistance of Chlorella JB17, and identifies a NaHCO3-tolerance gene that could be used for crop breeding and genetic modification of microalgae for biofuel production.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yasin Torres-Tiji, Francis J. Fields, Yanping Yang, Vanessa Heredia, Svein Jarle Horn, Saumya R. Keremane, Moonsoo M. Jin, Stephen P. Mayfield
Summary: Microalgae have been recognized as an alternative platform for producing high-quality biomass and bioproducts. This study examines the potential of the microalga Chlamydomonas reinhardtii to produce a complex human recombinant protein in a high-density heterotrophic culture. The results show that C. reinhardtii is a viable option for producing complex recombinant proteins at high concentrations.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Antonia Schad, Sonja Roessler, Raimund Nagel, Heiko Wagner, Christian Wilhelm
Summary: Research has shown that efficient excretion of glycolate can be achieved by mutating the carbon concentrating mechanisms and the C2 cycle in Chlamydomonas reinhardtii. This is significant for the production of chemical building blocks and the advancement of algal-based biotechnology.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Rulan Jiang, Miller Tran, Bo Lonnerdal
Summary: The study shows that recombinant bovine OPN and recombinant human OPN exhibit similar effects to bovine milk OPN and mouse milk OPN on stimulating infant development.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ashish K. Maurya, Piali Sengupta
Summary: The paper introduces a novel cilia regulator called XBX-4, which modulates the CCRK and MAK kinase pathway in C. elegans. Mutations associated with Joubert syndrome in the XBX-4 DUF3719 domain also result in cilia elongation.
Article
Multidisciplinary Sciences
Yi-Ping Hsiang, Yi-Ting Wang, Keng-Shiang Huang, Ting-Yu Huang, Mi-Hsueh Tai, Yu-Mei Lin, Chih-Hui Yang, Jei-Fu Shaw
Summary: The study designed a chlorophyll purification method using twice solvent extraction to enhance the purity of total chlorophylls and convert them into chlorophyllides. The purified chlorophyllides showed good biocompatibility and anticancer activity in breast cancer cell lines, suggesting their potential as a candidate for combination therapy with doxorubicin.
Article
Electrochemistry
Adnan Sayegh, Luca A. Perego, Marc Arderiu Romero, Louis Escudero, Jerome Delacotte, Manon Guille-Collignon, Laurence Grimaud, Benjamin Bailleul, Frederic Lemaitre
Summary: This study focuses on extracting energy from oxygenic photosynthesis by rerouting electron flow using intact photosynthetic organisms to generate photocurrent. Researchers found that some quinones can interact with the embedded photosynthetic chain, but the balance between toxicity and bioelectricity production needs to be considered.
Article
Immunology
Tiffany Kirkaldy Spaanager Sztuk, Neil Marcus Rigby, Lasse Norskov-Nielsen, Stef J. Koppelman, Ana Isabel Sancho, Niels-Peter Hell Knudsen, Justin Marsh, Philip Johnson, Shashank Gupta, Alan Robert Mackie, Jeppe Madura Larsen, Katrine Lindholm Bogh
Summary: This study compared the preventive effect of allergen-specific immunotherapy through different routes of peanut allergen delivery in rats. Results showed that high dose oral and sublingual administration, as well as high and medium dose intragastric administration, prevented peanut allergy. Therefore, the route of allergen delivery plays a significant role in preventing peanut allergy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Allergy
Marie Bastin, Warner W. W. Carr, Carla M. M. Davis, David M. M. Fleischer, Jay A. A. Lieberman, S. Shahzad Mustafa, Thibault Helleputte, Timothee Bois, Dianne E. E. Campbell, Todd D. D. Green, Matthew Greenhawt
Summary: This study examined the serum-specific IgG4 and IgE levels in peanut-allergic children receiving Viaskin Peanut treatment, and found that IgG4 rise most clearly differentiated treatment responders from non-responders. IgG4/IgE ratios >20.1 and the combination of Ara h 1 and peanut IgG4/IgE showed moderate ability to predict treatment response.
Article
Genetics & Heredity
Jihye Yeon, Stephen M. M. Miller, Wipawee Dejtisakdi
Summary: Microalgae are a promising platform for producing valuable commercial products, and this study developed synthetic operon vectors to express multiple proteins from a single chloroplast transcription unit. The results showed successful expression of two proteins, but some coding sequences did not work well in synthetic operons in this alga.
Review
Biotechnology & Applied Microbiology
Ke Ma, Lei Deng, Haizhen Wu, Jianhua Fan
Summary: Microalgae are widely distributed organisms in nature that play a vital role in reducing industrial carbon emissions. They have the unique advantage of being able to carry out photosynthesis and harbor both prokaryotic and eukaryotic expression systems, making them a popular host for recombinant proteins. This article focuses on the latest technological progress in selectable markers for genetic engineering of microalgae, particularly the model species Chlamydomonas reinhardtii. The development of genetic tools and strategies has made it more feasible to develop microalgae chloroplasts as green factories for producing recombinant proteins. However, further techniques and toolkits need to be developed for efficient and markerless editing of plastid genomes.
BIORESOURCES AND BIOPROCESSING
(2022)
Article
Allergy
Jennifer S. Chen, Jessica D. S. Grassmann, Uthaman Gowthaman, Sam J. Olyha, Tregony Simoneau, M. Cecilia Berin, Stephanie C. Eisenbarth, Adam Williams
Summary: TFH13 cells regulate the production of high-affinity IgE in both mice and allergy patients, and can be identified in both species using optimized protocols.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Cell Biology
James A. Gregory, Emily Hoelzli, Rawan Abdelaal, Catherine Braine, Miguel Cuevas, Madeline Halpern, Natalie Barretto, Nadine Schrode, Guney Akbalik, Kristy Kang, Esther Cheng, Kathryn Bowles, Steven Lotz, Susan Goderie, Celeste M. Karch, Sally Temple, Alison Goate, Kristen J. Brennand, Hemali Phatnani
Article
Allergy
Amy M. Scurlock, A. Wesley Burks, Scott H. Sicherer, Donald Y. M. Leung, Edwin H. Kim, Alice K. Henning, Peter Dawson, Robert W. Lindblad, M. Cecilia Berin, Christine B. Cho, Wendy F. Davidson, Marshall Plaut, Hugh A. Sampson, Robert A. Wood, Stacie M. Jones
Summary: In this study, extended treatment with VP250 was well tolerated, and desensitization observed at week 52 persisted between weeks 52 and 130. Treatment success was predominantly observed in younger participants.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Editorial Material
Medicine, Research & Experimental
M. Cecilia Berin
Summary: The rise in food allergy prevalence suggests a role for environmental factors and intestinal microbiota. By comparing twins with different food allergies, researchers identified specific health-associated microbes and commensal species, providing insights for next-generation probiotic therapies.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Review
Allergy
M. Cecilia Berin
Summary: This review provides an overview of our current understanding of the mechanisms of food protein-induced enterocolitis syndrome (FPIES). Studies have shown marked activation of innate immune and neuroendocrine pathways during FPIES reactions, but specific recognition mechanisms of foods remain elusive.
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
(2021)
Article
Immunology
Xin Chen, M. Cecilia Berin, Virginia L. Gillespie, Hugh A. Sampson, David Dunkin
Summary: Epicutaneous immunotherapy inducing Tregs that produce IL-10 and TGF-beta shows promising potential as a treatment for IBD. OVA TCR enriched T cells from Foxp3-DTR mice prevent colitis, while those derived from IL-10(-/-) or CD4-dnTGFBRII mice do not.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Allergy
Daniel Lozano-Ojalvo, Scott R. Tyler, M. Cecilia Berin
Summary: This commentary summarizes a recent study by Tortola et al, which highlights the plasticity of T helper (Th) 17 cells and suggests their potential role as an important source of Th2 cells in specific inflammatory conditions.
Article
Allergy
M. Cecilia Berin, Daniel Lozano-Ojalvo, Charuta Agashe, Mary Grace Baker, J. Andrew Bird, Anna Nowak-Wegrzyn
Summary: The study found that FPIES challenges led to significant elevation of inflammatory mediators and cytokines, unique IL-17 signature, and activation of innate lymphocytes.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Review
Allergy
Nicole Ramsey, M. Cecilia Berin
Summary: Research on the immune basis and treatment of food allergy is rapidly advancing, with a focus on local tissue factors and cytokines in the development and treatment of food allergy. The role of IL-13 and the targeting of IL-4 and IL-13 are significant in the treatment of food allergy, while low specific IgE and elevated specific IgG4 are predictive of treatment outcomes.
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2021)
Review
Multidisciplinary Sciences
Justine C. Noel, M. Cecilia Berin
Summary: Allergic diseases, such as asthma, food allergy, eczema, and allergic rhinitis, are becoming more common. Both adaptive and innate immune systems play important roles in the pathophysiology of allergies. The communication between epithelial cells and mononuclear phagocytes is crucial in controlling allergy and tolerance.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Editorial Material
Allergy
M. Cecilia Berin
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Allergy
M. Cecilia Berin, Charuta Agashe, A. Wesley Burks, David Chiang, Wendy F. Davidson, Peter Dawson, Alexander Grishin, Alice K. Henning, Stacie M. Jones, Edwin H. Kim, Donald Y. M. Leung, Madhan Masilamani, Amy M. Scurlock, Scott H. Sicherer, Robert A. Wood, Hugh A. Sampson
Summary: This study examined the relationship between food allergen-specific T-cell response and food allergy. It found that food-specific type 2 T cells at baseline can predict the threshold of reactivity and response to immunotherapy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Allergy
Daniel Lozano-Ojalvo, Xin Chen, David Dunkin, Charuta Agashe, Mary Grace Baker, J. Andrew Bird, Elena Molina, Anna Nowak-Wegrzyn, M. Cecilia Berin
Summary: Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergy with distinct symptoms and a poorly understood link between immune activation and symptoms. This study used untargeted metabolomics to identify novel pathways associated with FPIES reactions and found that the activation of the purinergic pathway may connect inflammation and vomiting through serotonin release from gastric and duodenal mucosa.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Review
Allergy
Cecilia Berin
Summary: There has been rapid growth in the field of immunoglobulin E-mediated food allergy therapeutics, with 1 US Food and Drug Administration-approved therapy in 2020 and several others in various stages of investigation. Oral immunotherapy is the approach with the longest track record of study and provides desensitization for most individuals undertaking the therapy. However, the therapy must be maintained for continued clinical protection, and adverse effects of the therapy are frequent. There is a need to improve allergen immunotherapy safety and durability and to provide a treatment that can target multiple food allergies.
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
(2023)
Review
Immunology
M. Cecilia Berin
Summary: IgE-mediated food allergy affects 6-8% of the population in the United States. Type 2 immune responses play a central role in the pathogenesis of food allergy, with heterogeneity observed in type 2 CD4(+) T cell responses. Oral immunotherapy has limited effectiveness in targeting type 2 immunity in food allergy, but new therapeutics targeting different levels of type 2 immunity are being developed. Understanding the heterogeneity of type 2 immunity and the contribution of different cell types is crucial for the development of effective treatments for food allergy.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)