Article
Plant Sciences
Megan McCaghey, Dandan Shao, Jake Kurcezewski, Ally Lindstrom, Ashish Ranjan, Steven A. Whitham, Shawn P. P. Conley, Brett Williams, Damon L. Smith, Mehdi Kabbage
Summary: Targeting the key enzyme responsible for oxalic acid production in Sclerotinia sclerotiorum using BPMV vectors and environmental RNAs can decrease its expression and enhance soybean resistance to the pathogen.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Magdalena Serafin-Andrzejewska, Marcin Kozak, Andrzej Kotecki
Summary: Pod sealant application has a positive impact on the quantity and quality traits of field peas, improving traits such as number of pods per plant, seed weight per pod, seed yield, protein content in seeds, and total protein productivity. However, the effect on number of seeds per pod is not significant, and the interaction between cultivar and pod sealant application showed significant effects on certain traits in 2019.
Article
Plant Sciences
Andrey D. Bovin, Olga A. Pavlova, Aleksandra V. Dolgikh, Irina V. Leppyanen, Elena A. Dolgikh
Summary: This study investigates the role of heterotrimeric G-proteins in nodulation in Medicago truncatula and Pisum sativum plants. The research provides evidence that G-protein beta subunits are involved in symbiosis development, as their suppression significantly reduces nodule formation. The expression of G-protein beta subunit is detected in developing nodule primordia and young nodules, with the most intense staining in the meristem and invasion zone. Protein-protein interaction studies suggest a cross-talk between G-protein- and phospholipase C-mediated signaling pathways.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Nawab Singh, Raufur Rahman Khan, Weihui Xu, Steven A. Whitham, Liang Dong
Summary: This study presents a miniaturized sensor for rapid, selective, and sensitive detection of bean pod mottle virus (BPMV) in soybean plants. The sensor employs molecularly imprinted polymer technology to generate BPMV-specific nanocavities in porous polypyrrole. The sensor outperforms conventional methods in terms of sensitivity, concentration range, detection time, and detection limit. It can distinguish infected plants from non-infected ones and reveal the spatial distribution of virus concentration across leaves. Moreover, the sensor can be easily restored and reactivated for multiple uses.
Article
Plant Sciences
Federico Betti, Maria Jose Ladera-Carmona, Daan A. Weits, Gianmarco Ferri, Sergio Iacopino, Giacomo Novi, Benedetta Svezia, Alicja B. Kunkowska, Antonietta Santaniello, Alberto Piaggesi, Elena Loreti, Pierdomenico Perata
Summary: Plants can uptake exogenous RNA to affect gene expression in nearby plants via RNAi mechanism, indicating a role of miRNA as signaling molecules for plant-to-plant communication. This communication mechanism requires the production of secondary siRNAs.
Article
Plant Sciences
Sasiprapa Sriwichai, Tidarat Monkham, Jirawat Sanitchon, Sanun Jogloy, Sompong Chankaew
Summary: This study aimed to evaluate the dual-purpose of winged beans, and found that the winged bean accessions W099 and W018 performed well in Thailand experiments, suitable for both pod and tuber yields, with high protein content and significant levels of fiber, energy, and minerals.
Article
Multidisciplinary Sciences
Qian-Qian Yang, Xing-Xing Zhao, Dao Wang, Peng-Jun Zhang, Xue-Nan Hu, Shuang Wei, Jing-Yuan Liu, Zi-Hong Ye, Xiao-Ping Yu
Summary: In this study, a fast and accurate detection method for Bean pod mottle virus (BPMV) was developed. The method targeting the coat protein gene of BPMV was highly specific, and had the advantages of visual determination and convenience.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Sun-Ju Rhee, Yoon Jeong Jang, Jun-Young Park, Jisu Ryu, Gung Pyo Lee
Summary: In this study, we developed a VIGS vector pCF93 for high-throughput analysis of gene function and identified candidate genes related to male sterility in watermelon. Silencing these genes revealed phenotypic features and developmental defects associated with male sterility. Our findings suggest that our VIGS system will facilitate high-throughput analysis of gene function in watermelons.
Article
Virology
Chouaib Meziadi, Julie Lintz, Masoud Naderpour, Charlotte Gautier, Sophie Blanchet, Alicia Noly, Ariane Gratias-Weill, Valerie Geffroy, Stephanie Pflieger
Summary: Elevated temperatures have a significant impact on plant-virus interactions, with resistance mediated by R-BPMV showing temperature-dependent changes. While R-BPMV remains heat-stable at 35 degrees Celsius, susceptible genotypes exhibit increased BPMV infection under elevated temperatures.
Article
Biology
Evelyn L. Eastwood, Kayla A. Jara, Susanne Bornelov, Marzia Munafo, Vasileios Frantzis, Emma Kneuss, Elisar J. Barbar, Benjamin Czech, Gregory J. Hannon
Summary: In animal gonads, the PIWI-interacting RNA (piRNA) pathway guards genome integrity by silencing transposon insertions co-transcriptionally. In Drosophila ovaries, the Piwi protein loaded with piRNA identifies nascent transposon transcripts and induces heterochromatin formation through the Panoramix-induced co-transcriptional silencing (PICTS) complex, which consists of Panoramix, Nxf2 and Nxt1. The study reveals that the dynein light chain LC8/Cut-up (Ctp) is a crucial component of the PICTS complex, essential for maintaining transposon silencing and repressive chromatin marks at transposon loci.
Article
Plant Sciences
Estefania Carrillo-Perdomo, Jean-Bernard Magnin-Robert, Blandine Raffiot, Chrystel Deulvot, Matthieu Floriot, Isabelle Lejeune-Henaut, Pascal Marget, Judith Burstin, Nadim Tayeh, Gregoire Aubert
Summary: In this study, QTL mapping in faba beans resulted in discovering five genomic regions associated with frost tolerance. The researchers also identified a major and stable QTL on the long-arm of LGI. Comparison with other legume species showed that the genetic control of frost tolerance in faba bean might be conserved. The study also provided four new RILs with high and stable tolerance for breeding programs.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Virology
Saeideh Ebrahimi, Omid Eini, Alexandra Bassler, Arvid Hanke, Zeynep Yildirim, Michael Wassenegger, Gabi Krczal, Veli Vural Uslu
Summary: Beet curly top Iran virus (BCTIV) has a unique genome organization and its V2 and Rep genes can suppress different types of gene silencing. Rep can suppress systemic silencing while V2 cannot, as demonstrated by experiments.
Article
Entomology
Jane C. Todd, Lucy R. Stewart, Margaret G. Redinbaugh, Jennifer R. Wilson, Arash Rashed
Summary: This study used electropenetography to examine the effects of two plant viruses on soybean aphid feeding behavior. The results showed little impact of soybean mosaic virus, but significant differences were observed in aphids feeding on bean pod mottle virus-infected plants. These findings contribute to further research on virus transmission and the impact on soybean aphid populations.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2022)
Article
Agronomy
Surjeet Kumar Arya, Sanchita Singh, Santosh Kumar Upadhyay, Vipin Tiwari, Gauri Saxena, Praveen C. Verma
Summary: This study provides evidence for the effectiveness of using RNAi technology to target the Kruppel homologue-1 gene in a hemipteran pest, P. solenopsis. The results show significant downregulation of gene expression for Kruppel homologue-1, demonstrating the potential of RNAi technology in pest control.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
S. Karthigai Devi, Geetika Banta, Vikas Jindal
Summary: RNA interference (RNAi) is a technique used to study gene functions in insects such as whitefly. The delivery of dsRNA into the insects is crucial for the effectiveness of RNAi, and this study developed a reliable system for delivering dsRNA to whitefly nymphs.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Agronomy
C. Le May, C. Onfroy, A. Moussart, D. Andrivon, A. Baranger, M. L. Pilet-Nayel, G. Vandemark
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2018)
Article
Plant Sciences
Aurore Desgroux, Valentin N. Baudais, Veronique Aubert, Gwenola Le Roy, Henri de Larambergue, Henri Miteul, Gregoire Aubert, Gilles Boutet, Gerard Duc, Alain Baranger, Judith Burstin, Maria Manzanares-Dauleux, Marie-Laure Pilet-Nayel, Virginie Bourion
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Plant Sciences
Marijn Knip, Manon M. S. Richard, Lisa Oskam, Hylco T. D. Van Engelen, Thomas Aalders, Frank L. W. Takken
MOLECULAR PLANT PATHOLOGY
(2019)
Article
Plant Sciences
Anne Quillevere-Hamard, Gwenola Le Roy, Anne Moussart, Alain Baranger, Didier Andrivon, Marie-Laure Pilet-Nayel, Christophe Le May
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Plant Sciences
Clarice J. Coyne, Lyndon D. Porter, Gilles Boutet, Yu Ma, Rebecca J. McGee, Angelique Lesne, Alain Baranger, Marie-Laure Pilet-Nayel
Article
Ecology
Maxime Bonhomme, Maria Ines Fariello, Helene Navier, Ahmed Hajri, Yacine Badis, Henri Miteul, Deborah A. Samac, Bernard Dumas, Alain Baranger, Christophe Jacquet, Marie-Laure Pilet-Nayel
Article
Plant Sciences
Estelle Billard, Anne Quillevere-Hamard, Clement Lavaud, Maire Laure Pilet-Nayel, Christophe Le May
JOURNAL OF PHYTOPATHOLOGY
(2019)
Article
Green & Sustainable Science & Technology
Marie-Benoit Magrini, Guillaume Cabanac, Matteo Lascialfari, Gael Plumecocq, Marie-Josephe Amiot, Marc Anton, Gaelle Arvisenet, Alain Baranger, Laurent Bedoussac, Jean-Michel Chardigny, Gerard Duc, Marie-Helene Jeuffroy, Etienne-Pascal Journet, Herve Juin, Colette Larre, Hugues Leiser, Valerie Micard, Dominique Millot, Marie-Laure Pilet-Nayel, Christophe Nguyen-The, Tristan Salord, Anne-Sophie Voisin, Stephane Walrand, Jacques Wery
Article
Genetics & Heredity
Manon M. S. Richard, Marijn Knip, Thomas Aalders, Machiel S. Beijaert, Frank L. W. Takken
FRONTIERS IN GENETICS
(2020)
Article
Plant Sciences
Anne Quillevere-Hamard, Gwenola Le Roy, Angelique Lesne, Christophe Le May, Marie-Laure Pilet-Nayel
Summary: The study evaluated the aggressiveness of diverse French isolates of Aphanomyces euteiches on Near Isogenic Lines (NILs) carrying different resistance QTLs. The isolates were categorized into three groups based on aggressiveness levels, while the pea lines were classified into three groups based on their resistance levels to the isolates carrying different resistance QTLs.
Article
Plant Sciences
Manon M. S. Richard, Ariane Gratias, Juan C. Alvarez Diaz, Vincent Thareau, Stephanie Pflieger, Chouaib Meziadi, Sophie Blanchet, William Marande, Elena Bitocchi, Roberto Papa, Phillip N. Miklas, Valerie Geffroy
Summary: The Co-x gene confers total resistance to a virulent strain of Colletotrichum lindemuthianum, providing a critical defense mechanism against anthracnose in common beans.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Manon M. S. Richard, Marijn Knip, Joelle Schachtschabel, Machiel S. Beijaert, Frank L. W. Takken
Summary: Plant intracellular immune receptors can trigger a hypersensitive response (HR) upon pathogen perception, but extreme resistance (ER) to viruses like potato virus X (PVX) does not involve cell death. Different variants of the immune receptor Rx1 have varying effects on viral infection, with wild-type Rx1 compromising CP protein accumulation. This suggests that translational arrest of viral genes is a major component of ER and is necessary for resistance to PVX, unlike the HR.
Article
Plant Sciences
Tieme A. Helderman, Laurens Deurhof, Andre Bertran, Manon M. S. Richard, Richard Kormelink, Marcel Prins, Matthieu H. A. J. Joosten, Harrold A. van den Burg
Summary: A VIGS screen was used to identify host factors for TSWV, resulting in the discovery of a gene family targeting the ribosomal protein S6 (RPS6) that provides resistance against the virus.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Genetics & Heredity
Juan C. Alvarez-Diaz, Manon M. S. Richard, Vincent Thareau, Gianluca Teano, Christine Paysant-Le-Roux, Guillem Rigaill, Stephanie Pflieger, Ariane Gratias, Valerie Geffroy
Summary: This study identified and analyzed the components and expression patterns of the RNA-guided silencing pathway in the non-model plant common bean. The findings provide insights into the biological processes and stress responses of common bean, which is important for its growth and development.
Article
Genetics & Heredity
Manon M. S. Richard, Ariane Gratias, Vincent Thareau, Kyung Do Kim, Sandrine Balzergue, Johann Joets, Scott A. Jackson, Valerie Geffroy
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)