Article
Entomology
Vladimir A. Lukhtanov, Nazar A. Shapoval, Alexander V. Dantchenko, Wolfgang Eckweiler
Summary: The study analyzed the partitioning of species groups in the butterfly subgenus Polyommatus (Agrodiaetus) using three datasets. It was found that the DNA barcoding approach alone was not able to reveal the phylogenetic structure, but the combined analysis of mitochondrial and nuclear sequences was successful in identifying species groups.
Article
Biochemistry & Molecular Biology
Lynne M. Christianson, Shannon B. Johnson, Darrin T. Schultz, Steven H. D. Haddock
Summary: Despite historical difficulties in studying ctenophores, new primers have successfully amplified COI fragments, highlighting high diversity and supporting ecological and morphological differences among species. These new tools provide important advancements for researchers in exploring ctenophore diversity on a global scale.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Multidisciplinary Sciences
Muhammad Ashfaq, Arif M. Khan, Akhtar Rasool, Saleem Akhtar, Naila Nazir, Nazeer Ahmed, Farkhanda Manzoor, Jayme Sones, Kate Perez, Ghulam Sarwar, Azhar A. Khan, Muhammad Akhter, Shafqat Saeed, Riffat Sultana, Hafiz Muhammad Tahir, Muhammad A. Rafi, Romana Iftikhar, Muhammad Tayyib Naseem, Mariyam Masood, Muhammad Tufail, Santosh Kumar, Sabila Afzal, Jaclyn McKeown, Ahmed Ali Samejo, Imran Khaliq, Michelle L. D'Souza, Shahid Mansoor, Paul D. N. Hebert
Summary: This study used DNA barcoding to survey the insect diversity in Pakistan and found that the knowledge about insect species in Pakistan is limited. Most species can only be classified into order and family, with only a small portion classified into genus and species. The study also found that a majority of the insect species in Pakistan are unique to the country, but some species are also reported in neighboring countries.
Article
Engineering, Marine
Bo Liu, Yali Yan, Nan Zhang, Huayang Guo, Baosuo Liu, Jingwen Yang, Kecheng Zhu, Dianchang Zhang
Summary: Species markers can be assessed quickly and accurately using DNA barcoding. In this study, we used DNA barcoding to investigate the parrotfish family Scaridae in Hainan, and found that DNA barcoding can be an effective tool for monitoring, protecting, and managing fisheries, as well as for addressing taxonomic issues.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Veterinary Sciences
Luis M. Hernandez-Triana, Javier A. Garza-Hernandez, Aldo Ortega Morales, Sean W. J. Prosser, Paul D. N. Hebert, Nadya Nikolova, Elsa Barrero, Erick de J. de Luna-Santillana, Vicente H. Gonzalez-Alvarez, Ramon Mendez-Lopez, Rahuel J. Chan-Chable, Anthony R. Fooks, Mario A. Rodriguez-Perez
Summary: This study in Mexico analyzed mosquito feeding preferences and blood meal sources from RNA extracts, identifying multiple organisms from a single extract for the first time. A total of 1,634 mosquito specimens were collected, with four new species records for Mexico and nine new records for Chiapas State. High intraspecific divergence values were observed in certain taxa, and blood meal analysis revealed feeding preferences of different mosquito species.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Jeganathan Pandiyan, Arun Zachariah, Bathrachalam Chandramohan, Shahid Mahboob, Khalid A. Al-Ghanim, Zaib-Un-Nisa, Marcello Nicoletti, Marimuthu Govindarajan
Summary: This study used cytochrome c oxidase I (COI) to analyze the DNA barcodes and assess the relationship among waterbird species. The phylogenetic tree and sequence alignment of COI revealed genetic differences and conserved haplotypes among the species.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Zoology
David Selnekovic, Katarina Goffova, Jan Soltys, Eva Kovacova, Jan Kodada
Summary: The study describes a new species of Mordellistena (s. str.) platypoda found on the island of Ischia in Italy, based on morphological characters and genetic analysis. The analysis shows that M. platypoda is closely related to M. tarsata, but they differ significantly in vestiture colouration, presence of lateral ctenidia on the third metatarsomere, and presence of sexual dimorphism on the protibia. Interestingly, the new species is currently known only from Ischia and no additional specimens were found in museum material.
Article
Entomology
Wolfgang Paill, Stephan Koblmueller, Thomas Friess, Barbara-Amina Gereben-Krenn, Christian Mairhuber, Michael J. Raupach, Lukas Zangl
Summary: The study provides the first reliable record of Pterostichus adstrictus in the Austrian Alps, confirming its southernmost distribution edge. Further research demonstrates the seasonal occurrence and habitat preferences of P. adstrictus in the region.
Article
Ecology
Lijuan Wang, Zhihao Wu, Guiqing Sun, Hui Chen, Hongjun Liu, Feng You
Summary: This study conducted DNA barcoding analysis on 309 fish species in Chinese coastal waters, confirming that DNA barcoding is a reliable method for fish species identification. The establishment of a barcode library will facilitate the monitoring and management of marine fishery resources in China.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Iole Di Capua, Rosa D'Angiolo, Roberta Piredda, Carmen Minucci, Ferdinando Boero, Marco Uttieri, Ylenia Carotenuto
Summary: Zooplankton molecular analyses provide accurate species identification, complementing traditional taxonomy. This study integrated morphological and molecular identification methods to assess taxonomic diversity in the Mediterranean Sea. The findings contribute to our understanding of coastal biodiversity and lay the groundwork for future studies.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Zoology
Xiao-Jiang Guo, Rui Cheng, Shan Jiang, Da-Yong Xue, Hong-Xiang Han
Summary: This article provides a review and annotated catalogue of the Chinese species of the genus Ditrigona Moore, 1888. Four new species are described and two existing species are reclassified. A newly recorded species in China is also reported. Illustrations of the new and known species' habitus and genitalia are presented.
Article
Fisheries
Yo Su, Hsiu-Chin Lin, Hsuan-Ching Ho
Summary: This article describes a new species of the roughy fish genus Hoplostethus, which can be distinguished from its congeners by a combination of characters, and provides comparisons with similar species. DNA barcoding confirms the monophyly of the new species, which appears to be closely related to Hoplostethus japonicus.
JOURNAL OF FISH BIOLOGY
(2022)
Review
Zoology
Cheng-Qi Zhu, Xiao-Dong Xu, Ying Zhen
Summary: The Luciolinae genus Emeia was studied and classified in this research. Phylogenetic analysis based on DNA barcoding sequences determined the classification of Emeia genus and revealed a new species. A key for identifying Emeia species using males is provided in the article.
Review
Agriculture, Dairy & Animal Science
Saima Naz, Ahmad Manan Mustafa Chatha, Rifat Ullah Khan
Summary: DNA barcoding and mini barcoding using COI gene are effective methods for accurate identification of species and biodiversity. This study aims to develop a reference database of fishes and evaluate the applicability of COI gene for species identification. The observed sequence length for fish species in DNA barcoding was 500-700 base pairs, while for DNA mini barcoding, it was 80-650 base pairs. These methods not only describe known and unknown samples, but also detect previously un-sampled species. Overall, these methods are important for species identification, conservation, and management of biodiversity.
ANNALS OF ANIMAL SCIENCE
(2023)
Article
Environmental Sciences
Edward Good, Luke E. Holman, Antonio Pusceddu, Tommaso Russo, Marc Rius, Claudio Lo Iacono
Summary: Using environmental DNA (eDNA) and taxonomic identification of meiofauna communities, this study finds that benthic trawling has community-wide impacts on deep-sea assemblages, with different trawling intensities leading to distinct communities.
MARINE POLLUTION BULLETIN
(2022)
Article
Ecology
Carlo L. Seifert, Patrick Strutzenberger, Axel Hausmann, Konrad Fiedler
Summary: Our study found that the range size and fundamental dietary breadth of European geometrid moths vary with latitude, and these two characteristics may be drivers of increased species richness towards lower latitudes. This supports the idea that both characteristics should be studied simultaneously in order to uncover mechanisms structuring biodiversity patterns at the macroecological scale.
GLOBAL ECOLOGY AND BIOGEOGRAPHY
(2022)
Review
Zoology
Hossein Rajaei, Axel Hausmann, Robert Trusch
Summary: This study provides a detailed investigation of the species of the genus Rhodostrophia in Iran, identifying 15 valid species and analyzing their distribution and morphological characteristics.
Review
Zoology
Gyula M. Laszlo, Axel Hausmann
Summary: This paper describes two new species of the genus Morabia and proposes new combinations based on integrative taxonomic analyses. The morphological data and DNA barcoding were used for detailed descriptions and illustrations of the species, including the female genitalia.
Article
Zoology
Guido Govi, Gabriele Fiumi, Jerome Barbut, Stefano Scalercio, Axel Hausmann
Summary: DNA barcoding analyses revealed unexpected genetic divergence in Phragmatiphila nexa populations in Italy, indicating the existence of three cryptic species. Wing patterns and genital characteristics support the recognition of three different species along the Italian peninsula.
Article
Biodiversity Conservation
Ilari E. Saaksjarvi, Kari M. Kaunisto, Michael Sharkey, Shelby Stedenfeld, M. Alex Smith, Winnie Hallwachs, Daniel Janzen
Summary: Three new species of the previously monotypic genus Creagrura Townes from Central and South America are described, highlighting the unknown diversity of parasitoid insects in the tropics. Detailed information on host relationships of the two Costa Rican species is provided. It is inferred that the Creagrura wasps lay their eggs on exposed caterpillars at night, rather than on concealed caterpillars during daylight hours.
BIODIVERSITY DATA JOURNAL
(2022)
Article
Biodiversity Conservation
Pagi S. S. Toko, Bonny Koane, Kenneth Molem, Scott E. E. Miller, Vojtech Novotny
Summary: The elevation gradients in tropical rainforests represent global maxima of biodiversity and are important for community studies. We surveyed geometrid moths in Papua New Guinea and found high diversity, with molecular data playing an important role in taxonomy. The richness of species and beta diversity of communities showed interesting patterns along the elevation gradient.
INSECT CONSERVATION AND DIVERSITY
(2023)
Article
Evolutionary Biology
Reza Zahiri, Jeremy D. Holloway, Jadranka Rota, B. Christian Schmidt, Markku J. Pellinen, Ian J. Kitching, Scott E. Miller, Niklas Wahlberg
Summary: In this study, a molecular phylogenetic analysis of the family Euteliidae was conducted to clarify deep divergences and evolutionary relationships. The analysis utilized a dataset consisting of mitochondrial and nuclear DNA loci and applied model-based phylogenetic methods. The results revealed two subfamilies, Euteliinae and Stictopterinae, and two tribes, Stictopterini and Odontini. Taxonomic revisions were needed as several genera were found to be polyphyletic. New genera were described and taxonomic changes were established. The study also provided insights into the divergence times and larval hostplants for the family.
SYSTEMATIC ENTOMOLOGY
(2023)
Article
Zoology
Elton Orlandin, Ryan A. St Laurent, Monica Piovesan, Winnie Hallwachs, Isidro Chacon, Daniel Janzen, Eduardo Carneiro
Summary: Notodontidae is a group of Lepidoptera insects that includes over 4,000 species distributed worldwide, with more than half of them found in the Neotropics. The classification of Notodontidae is still unsettled due to incomplete global sampling, and many taxa are informally classified. This study describes the immature stages of Anurocampa mingens from Brazil and identifies two new species in the genus from Costa Rica based on morphology and mitochondrial DNA. The systematic position of Anurocampa is also discussed.
Article
Zoology
Dominic Wanke, Asghar Shirvani, Axel Hausmann, Leidys Murillo-Ramos, Pasi Sihvonen
Summary: This study determined the phylogenetic position of the genus Eumera using genetic markers and morphological traits. It concluded that Eumera should be classified under the tribe Prosopolophini and described a new species, Eumera rajaeii sp. nov. Wanke & Shirvani, based on molecular data and morphology. The distribution of this Iranian species was shown on a map, and external characters and male genitalia of three related Eumera species were illustrated.
Article
Biodiversity Conservation
Bernardo F. Santos, Meredith E. Miller, Margarita Miklasevskaja, Jaclyn T. A. McKeown, Niamh E. Redmond, Jonathan A. Coddington, Jessica Bird, Scott E. Miller, Ashton Smith, Sean G. Brady, Matthew L. Buffington, M. Lourdes Chamorro, Torsten Dikow, Michael W. Gates, Paul Goldstein, Alexander Konstantinov, Robert Kula, Nicholas D. Silverson, M. Alma Solis, Stephanie L. de Waard, Suresh Naik, Nadya Nikolova, Mikko Pentinsaari, Sean W. J. Prosser, Jayme E. Sones, Evgeny V. Zakharov, Jeremy R. deWaard
Summary: The use of DNA barcoding has revolutionized biodiversity science, but the lack of comprehensive and reliable reference libraries for poorly known taxa remains a challenge. This study utilized the collections of the Smithsonian's National Museum of Natural History to generate DNA barcoding sequences for terrestrial arthropod genera that were previously not recorded in major public sequence databases. The combination of Sanger and Next-Generation Sequencing approaches maximized sequence recovery and minimized cost. The obtained sequences provide valuable resources for future biodiversity research and can be accessed through various data portals.
BIODIVERSITY DATA JOURNAL
(2023)
Article
Biodiversity Conservation
Valerie Levesque-Beaudin, Meredith E. Miller, Torsten Dikow, Scott E. Miller, Sean W. J. Prosser, Evgeny V. Zakharov, Jaclyn T. A. McKeown, Jayme E. Sones, Niamh E. Redmond, Jonathan A. Coddington, Bernardo F. Santos, Jessica Bird, Jeremy R. deWaard
Summary: Natural history collections are important repositories of knowledge on species and biodiversity. Making this knowledge accessible to society is crucial, and the development of a workflow for 'museum harvesting' of Diptera specimens from the Smithsonian Institution's National Museum of Natural History is a significant step in this direction. The workflow includes on-site and off-site processing, DNA barcoding, and the subsequent taxonomic assignment of specimens.
BIODIVERSITY DATA JOURNAL
(2023)
Article
Entomology
Ryan A. St Laurent, Paul Z. Goldstein, James S. Miller, Amanda Markee, Hermann S. Staude, Akito Y. Kawahara, Scott E. Miller, Robert K. Robbins
Summary: This study presents the first molecular phylogeny of Cerurinae moths, confirming their monophyletic status. The origin of Cerurinae is inferred to be Africa, with subsequent colonization of the Palearctic, the Americas, Indomalaya, and Australasia during the Miocene. The global spread of Cerurinae may have been facilitated by the distribution of their primary caterpillar foodplants in the Salicaceae family. State-dependent diversification analyses suggest that cerurines diversified most rapidly in temperate climates.
INSECT SYSTEMATICS AND DIVERSITY
(2023)
Review
Ecology
Caitlin N. Terry, Aura M. Alonso-Rodriguez, Scott E. Miller, Catherine M. Hulshof
Summary: This study summarizes past research on Lepidoptera in Puerto Rico and proposes areas of priority for future ecological research. The study found that there is a lack of understanding in native moth taxonomy and recent research has focused mainly on short-term evaluations of agricultural pests. The study recommends prioritizing ecological research to better understand the diversity and distribution changes of Lepidoptera and to conserve this biologically and economically significant group.
Article
Zoology
Gyula M. Laszlo, Axel Hausmann, Timm Karisch
Summary: The Afrotropical taxa of the Racotis s.l. generic complex were revised using integrative taxonomical methods. A new genus, Afroracotis gen. n., was established to include the Afrotropical Racotis species based on evaluation of genital morphology and DNA barcode analyses. The new genus is subdivided into 5 subgenera: Afroracotis subgen. n., Herbuloracotis subgen. n., Rwandaracotis subgen. n., Zebracotis subgen. n., and Sokokeracotis subgen. n..
Article
Biodiversity Conservation
John W. Brown, Scott E. Miller
Summary: Two new species of Xenolepis from Papua New Guinea were described and illustrated. Their larval forms were collected from various plant species, including the first reported food plants for Xenolepis. The study also provided DNA barcodes and illustrations of the male genitalia of an undescribed species.
JOURNAL OF ASIA-PACIFIC BIODIVERSITY
(2023)