Article
Chemistry, Analytical
Thomas Meikopoulos, Olga Begou, Georgios Theodoridis, Helen Gika
Summary: A new method for quantitative analysis of four ceramide species in dried blood samples was developed using LC-MS/MS. The Capitainer device was utilized to provide accurate sample volumes, allowing for accurate determinations. The study showed the potential application of at-home blood microsampling for health monitoring.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Isabel Ten-Domenech, Alvaro Solaz-Garcia, Inmaculada Lara-Canton, Alejandro Pinilla-Gonzalez, Anna Parra-Llorca, Maximo Vento, Guillermo Quintas, Julia Kuligowski
Summary: The GSH-to-GSSG ratio is critical for intracellular redox status. A new LC-MS method for measuring GSH and GSSG in DBS samples was developed, providing a convenient alternative to liquid blood samples. The levels of GSH-NEM and GSSG measured in DBS samples were comparable to those obtained from liquid blood samples in clinical scenarios.
Article
Biochemical Research Methods
Wenhao Qu, Xianglian Li, Guidi Tian, Linsheng Liu, Liyan Miao
Summary: In this study, a sensitive rapid assay for the simultaneous detection of warfarin enantiomers in mouse dried blood spot samples was developed and validated. The method showed high precision and reliability, and the samples remained stable at room temperature.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Jens Martens-Lobenhoffer, Stefan Angermair, Stefanie M. Bode-Boeger
Summary: This study developed a method for the simultaneous quantification of ceftazidime and avibactam, and evaluated dried blood spots (DBS) as a matrix for improved stability of the drugs. The results showed that the stability of ceftazidime and avibactam was better in DBS compared to plasma, extending the maximal transport times to 24 hours for DBS samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Milena Rmandic, Ana Stajic, Jasna Jancic, Janko Samardzic, Nebojsa Jovic, Andelija Malenovic
Summary: A UHPLC-MS/MS method was developed and validated for the determination of zonisamide in DPS and DBS samples, showing its suitability for clinical application.
Article
Medical Laboratory Technology
Michael J. Binks, Amy S. Bleakley, Geetha Rathnayake, Susan Pizzutto, Anne B. Chang, Brett McWhinney, Jacobus Ungerer
Summary: The study developed and validated a new method for rapid and accurate measurement of vitamin D metabolites in DBS samples, using a "bridging strategy" to correct for sample-method bias. Results showed a high correlation of 25OHD3 concentrations between DBS and plasma samples.
CLINICA CHIMICA ACTA
(2021)
Article
Infectious Diseases
Qian Liu, Lanyu Liu, Yu Yuan, Feifan Xie
Summary: This study developed a dried blood spots (DBS) method for pharmacokinetic studies in pediatric patients. The method used the Capitainer(R) qDBS device to collect a 10 mu L volume of blood on a paper card, and quantified the concentrations of eight antibiotics using UHPLC-MS/MS. The DBS method was shown to be simple, robust, and suitable for children with limited blood sampling.
Article
Biochemical Research Methods
Zi-Xuan Guo, Fan Yang, Johannes N. van den Anker, Yi Zheng, Wei Zhao
Summary: Bortezomib is a proteinase inhibitor used to treat multiple myeloma and mantle cell lymphoma, with high incidence of adverse reactions and large inter-individual differences in plasma concentrations. A LC-MS/MS method for quantitative analysis of bortezomib in dried blood spot samples was developed, showing no significant differences between bortezomib concentrations in DBS samples and plasma samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Medical Laboratory Technology
Chengfang Tang, Xuefang Jia, Fang Tang, Sichi Liu, Xiang Jiang, Xiaoyuan Zhao, Huiying Sheng, Minzhi Peng, Li Liu, Yonglan Huang
Summary: The study developed a simple, effective and accurate method for screening and diagnosing Gaucher disease using dried blood spot (DBS) samples, with Lyso-Gb1 identified as a reliable biomarker for GD. By establishing a normal reference interval of Lyso-Gb1 in DBS through analyzing samples from healthy controls, the study also detected GD patients and other abnormal cases through DBS analysis.
CLINICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Xiaolan Huang, Xiaofen Liu, Yu Wang, Jing Zhang
Summary: A novel quantification method of polymyxin B in dried blood spots (DBS) was developed and successfully applied in clinic. The method showed good accuracy, precision, matrix effects, and stability.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Matteo Moretti, Francesca Freni, Claudia Carelli, Carlo Previdere, Pierangela Grignani, Claudia Vignali, Maria Cobo-Golpe, Luca Morini
Summary: A liquid chromatographic tandem mass spectrometric procedure was developed and validated for the detection and quantitative determination of cannabinoids and metabolites in dried urine spots (DUS). The method fulfilled validation parameters for both DUS and urine. DUS seems to provide a good alternative storage condition for urine that should be checked for the presence of cannabinoids and metabolites.
Article
Chemistry, Analytical
Jie Wu, Zenghe Li, Baorong Chen
Summary: A rapid and accurate UPLC-MS/MS method was developed for measuring 19 steroid hormones in dried blood spots and achieving traceable analysis. The method utilized a BEH C8 column and UPLC-MS/MS for separation, with a simple and accurate extraction process. The distribution ranges of the 19 steroid hormones in four healthy individuals were determined simultaneously using positive and negative ionization modes, and the standard curves showed good linearity with correlation coefficients of R-2=0.997. The developed method provides important insights into the distribution of steroid hormones and enables traceable analysis in dried blood samples.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Ethel D. Weld, Teresa L. Parsons, Ryann Gollings, Marybeth McCauley, Beatriz Grinsztejn, Raphael J. Landovitz, Mark A. Marzinke
Summary: We developed and validated a LC-MS/MS assay for quantifying CAB and RPV from DBS, and derived conversion factors to estimate plasma concentrations from spotted blood.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Letter
Oncology
Scott R. Ceresnak, Yaqi Zhang, Xuefeng B. Ling, Kuo Jung Su, Qiming Tang, Bo Jin, James Schilling, C. James Chou, Zhi Han, Brendan J. Floyd, John C. Whitin, Kuo Yuan Hwa, Karl G. Sylvester, Henry Chubb, Ruben Y. Luo, Lu Tian, Harvey J. Cohen, Doff B. Mcelhinney
Summary: This study aimed to evaluate the feasibility of using dried blood spot (DBS) for screening and classifying congenital heart disease (CHD). The study analyzed metabolites in DBS samples to identify metabolic abnormalities in CHD patients and discovered potential biomarkers for CHD assessment and subtype classification. The study demonstrated for the first time that it is feasible to validate metabolite profiling results using long-term stored DBS samples.
BIOMARKER RESEARCH
(2023)
Article
Biochemical Research Methods
Harsha K. Tripathy, Vinay Kiran, Ashok Zakkula, A. B. Vinod, Rama Murthy Bestha, Sreekanth Dittakavi, Ramesh Mullangi
Summary: In this research, a validated LC-MS/MS method was used to quantify copanlisib from mouse dried blood spots. The method was validated in line with FDA guidelines and used liquid-liquid extraction technique to extract copanlisib from the DBS. The optimized chromatographic conditions allowed for accurate determination of copanlisib concentration, regardless of hematocrit levels.
BIOMEDICAL CHROMATOGRAPHY
(2023)