Review
Urology & Nephrology
Joana R. Martins, Dominik Haenni, Milica Bugarski, Marcello Polesel, Claus Schuh, Andrew M. Hall
Summary: The development of intravital imaging with multiphoton microscopy has revolutionized kidney research by allowing real-time visualization of dynamic cellular behavior and organelle function, providing insights into structure-function relationships in unprecedented detail. Advances in technology and image analysis have overcome practical challenges, opening up huge new possibilities for studying cellular mechanisms and disease states in the kidney.
KIDNEY INTERNATIONAL
(2021)
Article
Endocrinology & Metabolism
Melia D. Matthews, Emily Cook, Nada Naguib, Ulrich B. Wiesner, Karl J. Lewis
Summary: Osteocytes, as the resident mechanosensory cells in bone, play a crucial role in skeletal homeostasis and adaptation. This study demonstrates the usage of ultra-small and bright C'Dots as a novel in vivo imaging tool for osteocytes, which improves intravital imaging capabilities. It reveals distinct sex differences in nanoparticle intracellular dynamics and clearance in osteocytes and provides the first evidence of osteocyte integrin endocytosis and recycling in vivo. The results offer novel insights in osteocyte biology and open up new lines of investigation previously unavailable in vivo.
Review
Immunology
Philippe Bousso, Capucine L. L. Grandjean
Summary: Modulating the immune system can be used to treat various diseases and also improve our understanding of immune responses. This review discusses how imaging techniques can help clarify the mechanism of action of immunomodulatory strategies and guide the development of new drugs.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Ahmad Shirazi, Tayebeh Sahraeibelverdi, Miki Lee, Haijun Li, Joonyoung Yu, Sangeeta Jaiswal, Kenn R. Oldham, Thomas D. Wang
Summary: We demonstrate a side-view dual axes confocal endomicroscope that can be repetitively inserted in hollow organs of genetically engineered mice for in vivo real-time imaging in both horizontal and vertical planes. Near infrared (NIR) excitation at λex = 785 nm was used, and a monolithic 3-axis parametric resonance scan mirror fabricated using micro-electromechanical systems (MEMS) technology enabled post-objective scanning in the distal end of a 4.19 mm diameter instrument. Torsional and serpentine springs were designed to switch the mode of imaging between vertical and horizontal planes by tuning the actuation frequency. This system achieved real-time in-vivo images with a depth of 310 μm and lateral and axial resolution of 1.75 and 7.5 μm, respectively, in both horizontal and vertical planes, allowing identification of individual cells and discrete mucosal structures.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Physics, Applied
Kevin Keomanee-Dizon, Matt Jones, Peter Luu, Scott E. Fraser, Thai V. Truong
Summary: ExD-SPIM is an improved light-sheet microscopy strategy that solves the limitation of capturing fluorescence signals in large volumes by extending the depth of field (DOF) of high-NA detection objectives to match the thickness of the illumination light sheet. Compared to conventional light sheet imaging, ExD-SPIM improves the signal-to-noise ratio and reduces the rate of photobleaching. In whole-brain activity imaging, ExD-SPIM enhances signal sensitivity and volumetric coverage.
APPLIED PHYSICS LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Johannes Rebling, Maya Ben-Yehuda Greenwald, Mateusz Wietecha, Sabine Werner, Daniel Razansky
Summary: A large-scale label-free optoacoustic microscopy approach has been developed for rapid and non-invasive imaging of tissue regeneration, providing new insights into angiogenesis and enabling automatic analysis of key features in wound angiogenesis. This approach is a versatile tool for preclinical research in tissue engineering and regenerative medicine, allowing for high-throughput and quantitative studies of vascular remodeling and response to pharmacological interventions in vivo.
Article
Chemistry, Multidisciplinary
Yingxian Zhang, Jincheng Zhong, Hui Cheng, Jie Huang, Zhenhui Li, Chi Zhang, Zhiang Gao, Zhourui Xu, Gaixia Xu, Ping Qiu, Ke Wang
Summary: By detailed measurements and comparison of the effective attenuation length (l(e)) in different excitation wavelengths, this study demonstrates that 3-photon microscopy at 1700 nm enables the deepest penetration in brain imaging in vivo.
NANOSCALE ADVANCES
(2023)
Review
Cell Biology
Nazli Alizadeh-Tabrizi, Stefan Hall, Christian Lehmann
Summary: Intravital microscopy is a unique imaging method that provides real-time insights into cellular functions without the need for tissue extraction. Despite specific challenges in lung imaging, advancements in surgical and optical techniques have enabled researchers to uncover mechanisms of leukocyte recruitment and function in models of pulmonary inflammation and infection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Juhee Pae, Johanne T. Jacobsen, Gabriel D. Victora
Summary: Germinal centers serve as the site for antibody affinity maturation, and multiphoton laser scanning microscopy has allowed insight into the dynamic biological processes within GCs. This technical advancement has greatly contributed to our understanding of GC biology.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Cardiac & Cardiovascular Systems
Adam Nyul-Toth, Stefano Tarantini, Jordan DelFavero, Feng Yan, Priya Balasubramanian, Andriy Yabluchanskiy, Chetan Ahire, Tamas Kiss, Tamas Csipo, Agnes Lipecz, Attila E. Farkas, Imola Wilhelm, Istvan A. Krizbai, Qinggong Tang, Anna Csiszar, Zoltan Ungvari
Summary: Age-related blood-brain barrier disruption and cerebromicrovascular rarefaction play important roles in the pathogenesis of vascular cognitive impairment and dementia (VCID) and Alzheimer's disease (AD). Recent advances in geroscience research have provided new interventions for preventing these diseases. Sensitive and easy-to-adapt imaging methods are urgently needed for longitudinal assessment of changes in BBB permeability and brain capillarization in aged mice.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Biology
Kevin Schilling, Yuankn Zhai, Zhuang Zhou, Bin Zhou, Edward Brown, Xinping Zhang
Summary: We examined the formation and specification of blood vessels at the osteogenic and angiogenic interface in murine cranial bone defect repair using advanced optical techniques. Our study revealed dynamic changes in vessel types and their coupling with osteoblast expansion and bone tissue deposition/remodeling during repair. We also found heterogeneous oxygen distributions in capillary vessels at the healing site, with endothelial cells coupling to osteoblasts preferentially using glycolysis while osteoblasts rely more on OxPhos and potentially consume more oxygen at the repair site.
Review
Pharmacology & Pharmacy
Jeffrey Momoh, Dimitrios Kapsokalyvas, Michael Vogt, Sjoerd Hak, Fabian Kiessling, Marc van Zandvoort, Twan Lammers, Alexandros Marios Sofias
Summary: Intravital microscopy (IVM) has broad applications in various fields, including biology, physiology, immunology, and drug development. This review provides an overview of the modalities, methods, and model organisms used in IVM, as well as its applications in nanomedicine and biological process studies.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Mattia Sarti, Maria Parlani, Luis Diaz-Gomez, Antonios G. Mikos, Pietro Cerveri, Stefano Casarin, Eleonora Dondossola
Summary: This study developed an integrated computational pipeline based on an innovative variant of the U-Net neural network to segment and quantify cellular and extracellular structures of interest. The software shows promise for automatically detecting the elements of the FBR, unraveling the complexity of this pathophysiological process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Huan Liu, Hellen Ishikawa-Ankerhold, Julia Winterhalter, Michael Lorenz, Mykhailo Vladymyrov, Steffen Massberg, Christian Schulz, Mathias Orban
Summary: The thrombopoietic activity of fetal liver megakaryocytes was found to be higher than that of yolk sac megakaryocytes. Fetal platelets are released mainly through membrane budding. This study also demonstrated the independence of embryonic thrombopoiesis from definitive hematopoiesis during early development.
Article
Oncology
Samuel Nussbaum, Mira Shoukry, Mohammed Ali Ashary, Ali Abbaszadeh Kasbi, Mizba Baksh, Emmanuel Gabriel
Summary: This review discusses recent advances in cancer imaging, including artificial intelligence, molecular imaging, and intravital imaging. These technologies have the potential to improve the detection, monitoring, and treatment of cancer.
Article
Urology & Nephrology
Ivan Morace, Robert Pilz, Giuseppina Federico, Richard Jennemann, Damir Krunic, Viola Nordstroem, Johanna von Gerichten, Christian Marsching, Ina Maria Schiessl, Johannes Muething, Christian Wunder, Ludger Johannes, Roger Sandhoff, Hermann-Josef Groene
KIDNEY INTERNATIONAL
(2019)
Review
Urology & Nephrology
Ina Maria Schiessl
SEMINARS IN NEPHROLOGY
(2020)
Article
Medicine, Research & Experimental
Hiroyuki Kadoya, Ning Yu, Ina Maria Schiessl, Anne Riquier-Brison, Georgina Gyarmati, Dorinne Desposito, Kengo Kidokoro, Matthew J. Butler, Chaim O. Jacob, Janos Peti-Peterdi
Article
Multidisciplinary Sciences
Hans I-Chen Harn, Sheng-Pei Wang, Yung-Chih Lai, Ben Van Handel, Ya-Chen Liang, Stephanie Tsai, Ina Maria Schiessl, Arijita Sarkar, Haibin Xi, Michael Hughes, Stefan Kaemmer, Ming-Jer Tang, Janos Peti-Peterdi, April D. Pyle, Thomas E. Woolley, Denis Evseenko, Ting-Xin Jiang, Cheng-Ming Chuong
Summary: The study compares laboratory and African spiny mice, revealing a link between Twist1 and differences in hair follicle regenerative behavior after wounding. Through investigation of tissue mechanics and molecular responses, it demonstrates opposing trends in spatial and temporal morphogenetic field formation in these two mouse models following wound stiffness landscapes.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Dorinne Desposito, Ina Maria Schiessl, Georgina Gyarmati, Anne Riquier-Brison, Audrey K. Izuhara, Hiroyuki Kadoya, Balint Der, Urvi Nikhil Shroff, Young-Kwon Hong, Janos Peti-Peterdi
Summary: Utilizing multiphoton microscopy of transgenic Cdh5-Confetti mice, researchers were able to track and study the remodeling and repair of endothelial cells in the kidney and other organs, providing insights into the mechanisms of endothelial cells under physiological and disease conditions.
Article
Urology & Nephrology
Naomi Issler, Sara Afonso, Irith Weissman, Katrin Jordan, Alberto Cebrian-Serrano, Katrin Meindl, Eileen Dahlke, Konstantin Tziridis, Guanhua Yan, Jose M. Robles-Lopez, Lydia Tabernero, Vaksha Patel, Anne Kesselheim, Enriko D. Klootwijk, Horia C. Stanescu, Simona Dumitriu, Daniela Iancu, Mehmet Tekman, Monika Mozere, Graciana Jaureguiberry, Priya Outtandy, Claire Russell, Anna-Lena Forst, Christina Sterner, Elena-Sofia Heinl, Helga Othmen, Ines Tegtmeier, Markus Reichold, Ina Maria Schiessl, Katharina Limm, Peter Oefner, Ralph Witzgall, Lifei Fu, Franziska Theilig, Achim Schilling, Efrat Shuster Biton, Limor Kalfon, Ayalla Fedida, Elite Arnon-Sheleg, Ofer Ben Izhak, Daniella Magen, Yair Anikster, Holger Schulze, Christine Ziegler, Martin Lowe, Benjamin Davies, Detlef Boeckenhauer, Robert Kleta, Tzipora C. Falik Zaccai, Richard Warth
Summary: This study identified a previously unrecognized autosomal recessive disorder characterized by sensorineural deafness and tubular proteinuria caused by a homozygous missense variant of EHD1. Functional studies in patients and animal models revealed that the mutation impairs receptor-mediated endocytosis in proximal tubules, leading to impaired reabsorption of filtered proteins.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Anders M. Kristensen, Luca Bordoni, Layla Pohl, Donato Sardella, Hanne Kidmose, Ina Schiessl
Article
Multidisciplinary Sciences
Elisabeth Grimm, Franciscus van der Hoeven, Donato Sardella, Katrin Willig, Ulrike Engel, Nisha Veits, Robert Engel, Elisabetta Ada Cavalcanti-Adam, Felix Bestvater, Luca Bordoni, Richard Jennemann, Kai Schoenig, Ina Maria Schiessl, Roger Sandhoff
Summary: Clathrin-mediated endocytosis is a well-studied cellular uptake pathway, but the dynamic cooperation of its components in vivo is still not well understood. This study generated a reporter mouse model to track endocytosis by fusing eGFP to clathrin light chain a. The fusion protein was expressed in all tissues and could be visualized using fluorescence microscopy. The functionality of the reporter was confirmed by recruitment to nanobeads recorded by TIRF microscopy.
Article
Physiology
Donato Sardella, Anders M. Kristensen, Luca Bordoni, Hanne Kidmose, Ali Shahrokhtash, Duncan S. Sutherland, Sebastian Frische, Ina Maria Schiessl
Summary: Serial intravital 2-photon microscopy is a powerful technique to assess tissue function and structure over time. However, it requires expertise and faces challenges such as motion artifacts. Here, we present a setup and image processing protocols that lower the barrier of entry to intravital microscopy of the kidney.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Physiology
Layla Pohl, Ina Maria Schiessl
Summary: Renal endothelial cells have the ability to remodel during processes like angiogenic sprouting, vessel repair, and transition into mesenchymal cells. These processes can play important roles in renal disease progression or regeneration, and their signaling pathways overlap to some extent. Angiogenesis is involved in wound healing after kidney injury and modulating it pharmaceutically has therapeutic potential. The contribution of differentiated endothelial cells and their role in kidney fibrosis, as well as the fate of EndoMT cells in kidney disease, are still uncertain. Further research is needed to classify cellular markers and understand the implications of angiogenesis and EndoMT in the kidney.
Article
Multidisciplinary Sciences
Luca Bordoni, Anders M. Kristensen, Donato Sardella, Hanne Kidmose, Layla Pohl, Sren Rasmus Palmelund Krag, Ina Maria Schiessl
Summary: This study tracks the injury and remodeling of tubules after acute kidney injury (AKI) and finds that the injury may spread over time. The longitudinal analysis of tubule injury, remodeling, and fate provides important insights into AKI pathology.
NATURE COMMUNICATIONS
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Elisabeth Grimm, Donato Sardella, Luca Bordoni, Katrin Willig, Felix Bestvater, Ina Maria Schiessl, Roger Sandhoff
Meeting Abstract
Dermatology
H. Harn, S. Wang, Y. Lai, B. Van Handel, Y. Liang, S. Tsai, I. Maria Schiessl, A. Sarkar, H. Xi, M. Hughes, S. Kaemmer, M. Tang, J. Peti-Peterdi, A. D. Pyle, T. E. Woolley, D. Evseenko, T. Jiang, C. Chuong
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Urology & Nephrology
Luca Bordoni, Donato Sardella, Ina Maria Schiessl
Meeting Abstract
Biochemistry & Molecular Biology
Ina Maria Schiessl, Dorinne Desposito, Audrey Izuhara, Georgina Gyarmati, Janos Peti-Peterdi
