期刊
PARASITOLOGY INTERNATIONAL
卷 65, 期 5, 页码 378-388出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2016.05.012
关键词
Toxoplasma gondii; Evacuole; Glycosylphosphatidylinositol-anchor; Cholesterol; Rhoptry protein
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [23117007, 24390103]
- Program for the Promotion to Improve Independent Research Environments for Young Scientists by Japan Science and Technology Agency
- Ministry of Health, Labor and Welfare of Japan [H23-Shinkosaiko-ippan-014]
- Japan Health Sciences Foundation [KHA1101]
- Naito Foundation
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Sumitomo Foundation
- Takeda Science Foundation
- Uehara Memorial Foundation
- Grants-in-Aid for Scientific Research [24390103, 15H04726] Funding Source: KAKEN
Host cell microdomains are involved in the attachment, entry, and replication of intracellular microbial pathogens. Entry into the host cell of Toxoplasma gondii and the subsequent survival of this protozoan parasite are tightly coupled with the proteins secreted from organelle called rhoptry. The rhoptry proteins are rapidly discharged into clusters of vesicles, called evacuoles, which are then delivered to parasitophorous vacuoles (PVs) or nucleus. In this study, we examined the roles of two host cell microdomain components, cholesterol and glycosylphosphatidylinositol (GPI), in evacuole formation. The acute depletion of cholesterol from the host cell plasma membrane blocked evacuole formation but not invasion. Whereas the lack of host cell GPI also altered evacuole formation but not invasion, instead inducing excess evacuole formation. The latter effect was not influenced by the evacuole-inhibiting effects of host cell cholesterol depletion, indicating the independent roles of host GPI and cholesterol in evacuole formation. In addition, the excess formation of evacuoles resulted in the enhanced recruitment of host mitochondria and endoplasmic reticulum to PVs, which in turn stimulated the growth of the parasite. (C) 2016 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license.
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