期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 21, 期 11, 页码 4450-4456出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201406237
关键词
electrospinning; ion exchange; mesoporous materials; nanofibers; peptides
资金
- Major State Basic Research Development Program of China (973 Program) [2013CB910702]
- National Natural Science Foundation of China [21475098, 91217309]
A simple method was developed for the preparation of ordered mesoporous silica-carbon composite nanofibers (OMSCFs). The OMSCFs exhibited high carbon content, continuously long fibrous properties, uniform accessible mesopores, and a large surface area. The OMSCFs were also found to have ion-exchange capacity. On the basis of the size-exclusion effect of the mesopores and mixed-mode hydrophobic/ion-exchange interactions, the OMSCFs were applied for rapid enrichment of endogenous peptides by using a miniaturized solid-phase extraction format. The adsorption mechanism was studied, and the eluting solution was optimized with standard peptide/protein solutions and protein digests. Employing a successive three-step elution strategy, followed by LC-MS/MS analysis, led to excellent performance with this approach in the extraction and prefractionation of peptides from human serum.
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