Article
Engineering, Biomedical
Jonathan H. Galarraga, Hannah M. Zlotnick, Ryan C. Locke, Sachin Gupta, Natalie L. Fogarty, Kendall M. Masada, Brendan D. Stoeckl, Lorielle Laforest, Miguel Castilho, Jos Malda, Riccardo Levato, James L. Carey, Robert L. Mauck, Jason A. Burdick
Summary: Soft norbornene-modified hyaluronic acid (NorHA) hydrogels were reinforced with polycaprolactone using melt electrowriting (MEW) to fabricate composite scaffolds. These scaffolds supported chondrogenesis and cartilage formation of encapsulated porcine mesenchymal stromal cells (pMSC) and exhibited suitable mechanical properties. Fixation of the composites with biodegradable pins or fibrin glue ensured implant retention, but the use of pins resulted in inferior cartilage repair compared to the use of glue.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Orthopedics
Yong Sang Kim, Dong Suk Suh, Dae Hyun Tak, Pill Ku Chung, Yoo Beom Kwon, Tae Yong Kim, Yong Gon Koh
Summary: The study aimed to investigate the outcomes of MSC implantation with concomitant HTO for varus knee OA. Results showed significant improvements in clinical and radiographic outcomes, with encouraging cartilage regeneration. Patient age and number of MSCs were found to be important factors influencing the outcomes.
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE
(2021)
Review
Orthopedics
Hamid Rahmatullah Bin Abd Razak, Katia Corona, Trifon Totlis, Li Yi Tammy Chan, Jose Filipe Salreta, Obeida Sleiman, Michele Vasso, Mike H. Baums
Summary: According to the available literature, MSCs implantation in patients with mild to moderate knee osteoarthritis is safe and provides short-term clinical improvement and satisfactory cartilage restoration, either as a standalone procedure or combined with HTO.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Review
Engineering, Biomedical
Xiao Xu, Limei Xu, Jiang Xia, Caining Wen, Yujie Liang, Yuanmin Zhang
Summary: Osteoarthritis (OA) is a common disease characterized by cartilage degeneration in middle-aged and elderly people. Currently, there is no effective treatment for OA apart from total joint replacement in advanced stages. Mesenchymal stem cells (MSCs) have shown great potential in regulating the cartilage microenvironment, promoting cartilage regeneration, and alleviating OA symptoms, making them a promising source of cells for OA therapy.
ACTA BIOMATERIALIA
(2023)
Article
Plant Sciences
Yong-Hong Yang, Che-Sheng Wen, Yung-Ling Kuo, Su-Ling Fu, Tung-Yi Lin, Chao-Ming Chen, Po-Kuei Wu, Wei-Ming Chen, Jir-You Wang
Summary: This study aimed to investigate the effects of GuiLu-ErXian Glue (GLEXG) on MSC-induced chondrogenesis and its potential mechanisms. The results showed that GLEXG enhanced the chondrogenic differentiation of MSCs in vitro and inhibited the senescence process of MSCs through exosomes release. In addition, GLEXG was able to rescue cartilage defects in a rat osteoarthritis knee model. Therefore, GLEXG has potential pharmacological effects in the treatment of osteoarthritis.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Riccardo Giorgino, Domenico Albano, Stefano Fusco, Giuseppe M. Peretti, Laura Mangiavini, Carmelo Messina
Summary: Osteoarthritis (OA) is a common chronic orthopedic disorder that primarily affects hips and knees. The variability in the prevalence of knee OA across studies highlights the heterogeneity of reported data worldwide. The pathogenetic mechanism of knee OA involves an inflammatory process in the synovial membrane, mediated by immune system activation and damage-associated molecular patterns (DAMPs). Mesenchymal stem cells (MSCs) offer promising therapeutic options, but unresolved issues remain regarding the optimal cell source and quantity. Further research is necessary to address these uncertainties. This review provides a comprehensive overview of the molecular mechanisms of cartilage damage in knee OA and discusses current regenerative medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Xiao-Na Xiang, Si-Yi Zhu, Hong-Chen He, Xi Yu, Yang Xu, Cheng-Qi He
Summary: Osteoarthritis is a common degenerative disease that causes high socioeconomic costs and disability rates. The knee joint is most commonly affected, characterized by progressive destruction of articular cartilage, loss of extracellular matrix, and inflammation. MSC-based therapy has been explored as a new regenerative treatment for knee osteoarthritis. However, the detailed functions and mechanisms of MSC-based therapy, especially for cartilage regeneration, have not been explained.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Ophthalmology
Jyoti Sharma, Ravi Kumar Sharma, Pankaj Gupta, Nalini Gupta, Nirbhai Singh
Summary: The use of temperature responsive surfaces as carriers resulted in uniform and homogeneous delivery of MSC sheets over the damaged corneal surface. Corneal transparency improved from day 7 onwards post-MSC transplantation in the rabbit chemically injured cornea. Complete re-epithelialization of the injured cornea was observed 15 days after MSC transplantation.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Editorial Material
Medicine, Research & Experimental
Jose Maria Lamo-Espinosa, Felipe Prosper, Juan F. Blanco, Fermin Sanchez-Guijo, Mercedes Alberca, Veronica Garcia, Margarita Gonzalez-Vallinas, Javier Garcia-Sancho
Summary: The study found that using 10, 40, and 100 million autologous cells for knee osteoarthritis treatment provided similar and long-lasting results, providing strong evidence for the efficacy of single-cell injection therapy.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Review
Cell Biology
Nikhil Agarwal, Christopher Mak, Christine Bojanic, Kendrick To, Wasim Khan
Summary: Cell-based therapies derived from adipose tissue, specifically AMSC therapy and SVF therapy, have been shown to be safe and effective treatments for knee OA in old adults, reducing pain and improving knee function over time.
Article
Biochemistry & Molecular Biology
Adel Hinsenkamp, Kiara Kun, Fatime Gajnut, Aliz Majer, Zsombor Lacza, Istvan Hornyak
Summary: Fibrin membranes are widely used in regenerative medicine due to their biocompatibility, biodegradability, high growth factor content, and cell attachment support. Cryoprecipitate isolation can increase fibrinogen and platelet concentration in plasma, affecting membrane thickness and growth factor content. However, membrane thickness does not significantly influence cell attachment, as demonstrated by live-dead staining and XTT measurements.
Review
Chemistry, Medicinal
Gollahalli Shivashankar Prajwal, Naveen Jeyaraman, Krishna Kanth, Madhan Jeyaraman, Sathish Muthu, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Manish Khanna, Eun Jung Oh, Kang Young Choi, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Tissue engineering and regenerative medicine provide a new approach for treating musculoskeletal diseases, utilizing MSCs and growth factors to improve the regenerative environment. However, the selection of MSCs source and the long-term and short-term effects of MSCs treatment remain uncertain.
Review
Biochemistry & Molecular Biology
Pengxu Wei, Ruixue Bao
Summary: Knee osteoarthritis is a common problem, especially in older individuals, and can eventually lead to disability. Injecting mesenchymal stem cells (MSCs) into the knee joint has been shown to induce cartilage regeneration in several studies. Additionally, understanding the anatomical, biomechanical, and biochemical changes associated with knee osteoarthritis can help develop appropriate interventions to delay disease progression and improve cartilage repair induced by MSC injection. Post-injection rehabilitation programs and related mechanisms are also discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Fenglei Chen, Shan Wang, Chenying Zeng, Su'an Tang, Huimin Gu, Ziming Wang, Jinteng Li, Pei Feng, Yunhui Zhang, Peng Wang, Yanfeng Wu, Huiyong Shen
Summary: This study reveals that circSERPINE2 plays a regulatory role in stem cell senescence and has therapeutic potential in alleviating aging-related disorders.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Zehao Zhang, Sheng Zhao, Zhaofeng Sun, Chuanxing Zhai, Jiang Xia, Caining Wen, Yuge Zhang, Yuanmin Zhang
Summary: Osteoarthritis (OA) is a common joint disorder causing pain and disability, with unsatisfactory current treatment outcomes. Recent studies have shown that mesenchymal stem cell-derived exosomes (MSC-Exos) have a significant impact on OA and could be a potential cell-free therapy. This review provides an overview of the synthesis, composition, and mechanisms of action of MSC-Exos in OA, and discusses their potential as a therapeutic tool and bioengineering approaches to enhance their therapeutic potential.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)