Article
Oncology
Min Sup Song, Pier Paolo Pandolfi
Summary: Members of the HECT family of E3 ubiquitin ligases have significant regulatory roles in PTEN function, subcellular localization, and levels, enabling the identification of genes involved in tumorigenesis and cancer susceptibility. These findings hold promise for the development of novel therapeutic approaches for cancer prevention and treatment, as well as other diseases.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Emma I. Kane, Donald E. Spratt
Summary: Ankyrin repeat (AR) domains are the most common repetitive structure in eukaryotic proteins, mediating specific protein-protein interactions and recognizing a variety of intracellular substrates. Proteins with AR domains play crucial roles in biological processes and their dysfunction is linked to neurological diseases and disorders. Understanding the structure and mechanism of key AR-containing proteins can provide insights into how they support ubiquitylation and subsequent signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Sunil Singh, Joel Ng, J. Sivaraman
Summary: The HECT E3 ligase family consists of three sub-families: NEDD4, HERC, and Other, with the latter being less explored. Members of the Other subfamily have diverse N-terminal regions preceding the conserved HECT domains, which influence the stability and activity of the proteins. They assemble atypical ubiquitin linkages and cooperate with E3 ligases from other subfamilies to form branched ubiquitin chains on substrates, making them potential candidates for therapeutic drug development.
PHARMACOLOGY & THERAPEUTICS
(2021)
Review
Oncology
Zongdong Yu, Hong Li, Jie Zhu, Haibiao Wang, Xiaofen Jin
Summary: Hepatocarcinogenesis involves complex genetic and epigenetic alterations, with abnormal activation or inactivation of E3 ligases playing a crucial role in the development and progression of HCC. Targeted therapies against aberrant E3 ligases show promising potential for future treatment strategies against HCC.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Jun Goto, Yoichiro Otaki, Tetsu Watanabe, Masafumi Watanabe
Summary: Despite medical advances, cardiac disease remains a significant health issue with high mortality rates. Maladaptive cardiac remodeling, like hypertrophy and fibrosis, is a risk factor for heart failure, underscoring the important role of ubiquitylation in cardiac disease development. HECT-type E3 ligases play a crucial role in ubiquitylation and have been implicated in a wide range of human diseases, including cardiac diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Kan Lu, Yonglong Pan, Zhao Huang, Huifang Liang, Ze-Yang Ding, Bixiang Zhang
Summary: The TRIM protein family is a versatile and highly conserved group of proteins that function as E3 ligases, regulating the development of hepatocellular carcinoma.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Microbiology
Bing Zhu, Jere W. McBride
Summary: Ehrlichia chaffeensis effector TRP120 regulates host cell processes by ubiquitinating transcription and fate regulators, promoting infection. The study identifies a novel TRP120 substrate, alpha-enolase, and demonstrates its ubiquitination by TRP120, leading to ENO-1 degradation and implications for metabolic reprogramming in infection. Trim120's role extends to auto-ubiquitination, recruiting E2 ubiquitin-conjugating enzymes for potential functional activation in infection.
Article
Oncology
Chen Xiong, Zhihuai Wang, Guifu Wang, Chi Zhang, Shengjie Jin, Guoqing Jiang, Dousheng Bai
Summary: The study identified CDC20 as a hub E3 ubiquitin-protein ligase gene in HCC, showing significantly higher expression in HCC tissues compared to normal tissues. High CDC20 expression was associated with poor prognosis and may serve as an independent risk factor in HCC. Additionally, CDC20 was correlated with immune cell infiltration, suggesting its potential as an immune-associated therapeutic target in HCC.
INVESTIGATIONAL NEW DRUGS
(2021)
Article
Cell Biology
Lei Feng, Jieqing Wang, Jianmin Zhang, Jingfang Diao, Longguang He, Chaoyi Fu, Hui Liao, Xiaoping Xu, Yi Gao, Chenjie Zhou
Summary: This study comprehensively analyzed the clinical significance of E3 ligases in pancreatic cancer, revealing that RNF223 promotes cancer growth by regulating cell metabolism. Through quantitative proteomics analysis, the study identified the involvement of RNF223 in metabolism-related pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Busra Turgu, Amal El-Naggar, Melanie Kogler, Luigi Tortola, Hai-Feng Zhang, Mariam Hassan, Michael M. Lizardo, Sonia H. Y. Kung, Wan Lam, Josef M. Penninger, Poul H. Sorensen
Summary: HACE1 destabilizes mTOR by targeting RAC1 within mTOR-associated complexes, leading to decreased mTORC1 and mTORC2 activity. HACE1 deficiency results in enhanced mTORC1/2 activity, which can be reversed by inhibiting RAC1. These findings reveal a unique ubiquitin-dependent process to control the activity of mTOR signaling complexes.
Review
Oncology
Aiqin Sun, Xianyan Tian, Yifei Chen, Wannian Yang, Qiong Lin
Summary: Gastric cancer is a highly malignant gastrointestinal tumor with high incidence and mortality. Ubiquitination modification of cellular signaling proteins plays an important role in gastric cancer tumorigenesis, progression, and prognosis. The HECT E3 ubiquitin ligases have been found to be involved in gastric cancer progression, but their role as tumor promoters or tumor suppressors in gastric cancer is controversial. This review focuses on recent discoveries about the role of HECT E3 ubiquitin ligases, especially members of the NEDD4 and other HECT E3 ligase subfamilies, in gastric cancer.
PATHOLOGY & ONCOLOGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Review
Oncology
Ruixia Ma, Maomao Zhao, Xin Zou, Jianye Zhou, Zhongtian Bai
Summary: miRNA polymorphisms play a role in the pathogenesis and prognosis of hepatocellular carcinoma (HCC), suggesting they could be potential targets for diagnosis and treatment of HCC.
Article
Toxicology
Chenghui Ren, Longfei Ren, Jun Yan, Zhongtian Bai, Lei Zhang, Honglong Zhang, Ye Xie, Xun Li
Summary: This study revealed that Cadmium exposure induces liver damage in rats, with significant DNA methylation changes observed in the high-dose exposure group, mainly in the gene promoter region. GO and KEGG analysis showed that the affected genes were enriched in biological processes responding to stimulus and metabolic pathways.
TOXICOLOGY LETTERS
(2021)
Article
Oncology
Bin Ma, Jing Li, Wen-Ke Yang, Mei-Gui Zhang, Xiao-Dong Xie, Zhong-Tian Bai
Summary: N-trans-Feruloyloctopamine (FO) inhibits the malignant phenotype of tumor cells by modulating AKT and EMT-related signals, making it a potential drug for HCC treatment. FO affects tumor cell proliferation and apoptosis, mainly through regulating PI3K-AKT and apoptosis-related signals, with BBC3, DDIT3, NOXA, and CDKN1A identified as novel targets for inducing HCC cell apoptosis.
ANALYTICAL CELLULAR PATHOLOGY
(2021)
Article
Toxicology
Honglong Zhang, Jun Yan, Ye Xie, Xuhong Chang, Junliang Li, Chenghui Ren, Jun Zhu, Longfei Ren, Kuo Qi, Zhongtian Bai, Xun Li
Summary: In this rat study, cadmium (Cd) was found to induce liver injury and inhibit the progression of liver cancer by activating and blocking mitophagy, depending on the dosage of Cd.
TOXICOLOGY LETTERS
(2022)
Article
Pharmacology & Pharmacy
Chenghui Ren, Longfei Ren, Jun Yan, Zhongtian Bai, Lei Zhang, Honglong Zhang, Ye Xie, Xun Li
Summary: The study revealed significant upregulation of lipid metabolism related genes in response to chronic cadmium exposure, with key genes such as Cyp1a1 and Pla2g2d being overexpressed. GO and KEGG analysis further supported the impact of cadmium on lipid metabolism. The results suggest a potential link between cadmium exposure and NAFLD through modulation of liver lipid metabolism.
Review
Oncology
Jiahui Xi, Yonghong Li, Hui Zhang, Zhongtian Bai
Summary: This review discusses the pathological features of gastric mucosa, characteristics of gastric microbiota, and the interactions between microorganisms and their hosts at different stages in Correa's cascade. By analyzing the relevant markers, important clues for early cancer reversal are provided, offering new insights for reducing the incidence of gastric cancer and improving precise treatment.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Biotechnology & Applied Microbiology
Yan Lin, Mengchao Yan, Zhongtian Bai, Ye Xie, Longfei Ren, Jiayun Wei, Dan Zhu, Haiping Wang, Yonggang Liu, Junqian Luo, Xun Li
Summary: This study successfully identified and modified the targeting peptide HSTP1 on Huc-MSC-derived exosomes, which showed superior targeting ability to aHSCs and enhanced the therapeutic effect on liver fibrosis. The engineered exosomes were more efficiently internalized by HSC-T6 cells and outperformed other types of exosomes in promoting HSC-T6 reversion to a quiescent phenotype. The in vivo experiments demonstrated the specific targeting ability of HSTP1-Exos to the aHSC region and its enhanced therapeutic effect.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Oncology
Ruixia Ma, Meigui Zhang, Jiahui Xi, Jing Li, Yinxia Ma, Binxiao Han, Tuanjie Che, Zhihui Ma, Jinhui Tian, Zhongtian Bai
Summary: This study provides a qualitative and quantitative evaluation of the research progress on protein post-translational modifications (PTMs) in tumorigenesis. The results show an increasing number of PTMs-related papers in the cancer field, with the highest number of publications from the United States. The research mainly focuses on mechanisms, histones, P53, and key technologies, with emerging topics such as tumor metabolism, autophagy, protein-protein interaction, and histone ubiquitination.
FRONTIERS IN ONCOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Junmin Xi, Li-Li Tian, Jiahui Xi, Desire Girimpuhwe, Chongfei Huang, Ruixia Ma, Xiaojun Yao, Danfeng Shi, Zhongtian Bai, Quan-Xiang Wu, Jianguo Fang
Summary: This study isolated and characterized two compounds from an endophytic fungus and investigated the antitumor mechanism of one compound AP. The study found that AP inhibits the growth of liver cancer cells by targeting the selenoprotein thioredoxin reductase (TrxR) and ultimately induces cell apoptosis and ferroptosis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Zhongtian Bai, Zhihui Ma, Libaihe Jing, Yonghong Li, Shufan Wang, Bin-Guo Wang, Yan Wu, Xiaotao Han
Summary: This paper proposes an SEIR-type epidemic model that considers the impact of face-mask wearing and vaccination to simulate the spread of COVID-19 and develop effective control measures. The model parameters are estimated based on South Korea's data. The results show that increasing the face mask wearing rate by 15% reduces the cumulative and newly confirmed cases by 83% and 90% respectively after 60 days. Increasing the vaccination rate by 15% also leads to reductions of 75% and 80% in the cumulative and newly confirmed cases after the same period. A combined measure of mask wearing and vaccination is suggested for more effective prevention and control, and the importance of promoting both measures is emphasized.
SCIENTIFIC REPORTS
(2023)
Review
Nanoscience & Nanotechnology
Ruili Zhou, Meigui Zhang, Jiahui Xi, Jing Li, Ruixia Ma, Longfei Ren, Zhongtian Bai, Kuo Qi, Xun Li
Summary: This review summarizes the recent advances in gold nanorods-based photothermal therapy (PTT). Functionalized gold nanorods have optimized biocompatibility and targeting properties. The multifunctional nanoplatform composite structure of gold nanorods achieves synergistic effects of PTT, photoacoustic imaging, and other therapeutic methods. Gold nanorods-based PTT has remarkable curative effects in tumor targeting, tumor metastasis inhibition, and photothermal tumor ablation.
NANOSCALE RESEARCH LETTERS
(2022)
Article
Oncology
Yan-Ping Wang, Ting Wei, Xiao Ma, Xiao-Liang Zhu, Long-Fei Ren, Lei Zhang, Fang-Hui Ding, Xun Li, Hai-Ping Wang, Zhong-Tian Bai, Ke-Xiang Zhu, Long Miao, Jun Yan, Wen-Ce Zhou, Wen-Bo Meng, Yu-Qin Liu
Summary: This study used UPLC-HDMS metabolomics to analyze plasma samples from patients with superficial gastritis, early gastric cancer, and advanced gastric cancer, revealing metabolic phenotype changes and potential biomarkers. The results showed differences in biomarkers related to amino acid, lipid, and fatty acid metabolism pathways, with glycerophosphocholine and neopterin identified as key biomarkers associated with gastric cancer progression. This method could be useful for biomarker identification and potential diagnosis of gastric cancer.
Article
Health Care Sciences & Services
Liting Zhang, Yonghong Li, Jianye Zhou, Junfeng Li, Chongxiang Tong, Jing Cai, Zengwei Yang, Shuyan Li, Jinhong Zhu, Meie Zhao, Zhongtian Bai, Xiaodong Xie, Tuo Chen
Summary: Through analyzing the laboratory variables of COVID-19 patients, CAP patients, and healthy individuals, it was found that the correlations between laboratory variables in the COVID-19 group were more complex compared to the other two groups. The combination of different laboratory indicators can effectively predict the development and progression of COVID-19 infection.
ANNALS OF PALLIATIVE MEDICINE
(2021)
Article
Oncology
Xun Li, Yongqiang Zhou, Jinjing Hu, Zhongtian Bai, Wenbo Meng, Lei Zhang, Xiaojing Song, Yongjian Wei, Jun Yan, Yihua Zhou
Summary: The study found that NRP1 promotes the population of liver CSCs and enhances HCC migration, potentially serving as a novel target for HCC treatment.
Article
Pharmacology & Pharmacy
Xingwen Han, Jingjing He, Zhan Wang, Zhongtian Bai, Peng Qu, Zhengdong Song, Wenji Wang
Summary: The study aimed to investigate the effect of gelatin on stabilizing silver nanoparticles (AgNPs) and their potential use in bone fracture healing. Results showed that AgNPs-loaded Gel hydrogels are non-toxic to osteoblasts and can improve the survival and spreading of these cells, suggesting their potential in nursing care for bone fractures.
