期刊
ONCOLOGY REPORTS
卷 35, 期 5, 页码 2643-2650出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2016.4639
关键词
Nanog; cervical cancer; mRNA
类别
资金
- Hubei University of Medicine Juvenile Scientific and Technological Creativity team [2014 CXZ06]
- Educational Foundation of Hubei Province [B2015484]
- Major Science and Technology Projects of infectious such as AIDS and viral hepatitis prevention and control, China [2013ZX10001-004-002-005]
Nanog is a pluripotency-related factor. It was also found to play an important role in tumorigenesis. To date, the mechanisms underlying cervical tumorigenesis still need to be elucidated. In the present study, Nanog mRNA was synthesized in vitro and transfected into HeLa cells. After mRNA transfection, the forced expressed of Nanog in HeLa cells led to markedly increased invasion, migration, resistance to chemotherapeutic agents and dedifferentiation. In a subcutaneous xenograft assay, these cells had significantly increased tumorigenic capacity. Real-time PCR indicated that Nanog-induced dedifferentiation was associated with increased expression of endogenous Oct4, Sox2 and FoxD3. In addition, the dedifferentiated HeLa cells acquired features associated with cancer stem cells (CSCs), such as multipotent differentiation capacity, and expression of CSC markers such as CD133. These data imply that Nanog is a positive regulator of cervical cancer dedifferentiation.
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