期刊
ONCOGENE
卷 36, 期 5, 页码 700-713出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/onc.2016.241
关键词
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资金
- Australian National Health and Medical Research Council (NHMRC) [1060698]
- Mater Foundation
- NHMRC Principal Research Fellowship
- Australian Government
- National Health and Medical Research Council of Australia [1060698] Funding Source: NHMRC
MUC13 is a transmembrane mucin glycoprotein that is over produced by many cancers, although its functions are not fully understood. Nuclear factor-kappa B (NF-kappa B) is a key transcription factor promoting cancer cell survival, but therapeutically targeting this pathway has proved difficult because NF-kappa B has pleiotropic functions. Here, we report that MUC13 prevents colorectal cancer cell death by promoting two distinct pathways of NF-kappa B activation, consequently upregulating BCL-XL. MUC13 promoted tumor necrosis factor (TNF)-induced NF-kappa B activation by interacting with TNFR1 and the E3 ligase, cIAP1, to increase ubiquitination of RIPK1. MUC13 also promoted genotoxin-induced NF-kappa B activation by increasing phosphorylation of ATM and SUMOylation of NF-kappa B essential modulator. Moreover, elevated expression of cytoplasmic MUC13 and NF-kappa B correlated with colorectal cancer progression and metastases. Our demonstration that MUC13 enhances NF-kappa B signaling in response to both TNF and DNA-damaging agents provides a new molecular target for specific inhibition of NFB activation. As proof of principle, silencing MUC13 sensitized colorectal cancer cells to killing by cytotoxic drugs and inflammatory signals and abolished chemotherapy-induced enrichment of CD133(+) CD44(+) cancer stem cells, slowed xenograft growth in mice, and synergized with 5-fluourouracil to induce tumor regression. Therefore, these data indicate that combining chemotherapy and MUC13 antagonism could improve the treatment of metastatic cancers.
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