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Oncology
Xinrui Rao, Biyuan Xing, Zilong Wu, Yawen Bin, Yunshang Chen, Yingzhuo Xu, Dong Zhou, Xiaoshu Zhou, Chuangyan Wu, Wang Ye, Weibin Chen, Geng Wang, Sheng Zhang, Xiaorong Dong, Rui Meng, Gang Wu, Rui Zhou
Summary: POLQ, an overexpressed DNA polymerase in non-small-cell lung cancer (NSCLC), is correlated with high tumor stage, poor prognosis, increased tumor mutational burden, and ALK and TP5 mutation status. Inhibition of POLQ impairs lung tumorigenesis and enhances radiosensitivity by prolonging the G2/M phase and promoting faster DNA damage repair. Novobiocin (NVB), a specific POLQ inhibitor, targets cancer cells without causing significant toxicity to normal pulmonary epithelial cells. These findings suggest that POLQ is a promising target for improving radiotherapy efficacy in NSCLC.
Article
Multidisciplinary Sciences
Huijun Xue, Amit Bhardwaj, Yandong Yin, Carel Fijen, Anastasiya Ephstein, Lianglin Zhang, Xia Ding, John M. Pascal, Todd L. VanArsdale, Eli Rothenberg
Summary: PARP inhibitors modulate the retention of PARP1 on DNA lesions through a two-step mechanism, involving competitive inhibition of NAD(+) binding and allosteric modulation. The retention potency of PARPi is predominantly determined by its ability to outcompete NAD(+) binding.
Article
Oncology
Qiang Xie, Shilei Zhao, Wenzhi Liu, Yanwei Cui, Fengzhou Li, Zhuoshi Li, Tao Guo, Wendan Yu, Wei Guo, Wuguo Deng, Chundong Gu
Summary: Abnormal expression of Y-box-binding protein-1 (YBX1) is associated with various cancer characteristics. Low expression of both YBX1 and Mucin-1 (MUC1) in lung adenocarcinoma is an independent predictor of prognosis and recurrence. YBX1 promotes stemness and metastasis by targeting MUC1 in lung adenocarcinoma.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Lianmin Zhang, Yuan Wang, Yongsheng Sha, Bin Zhang, Rui Zhang, Hua Zhang, Shilei Xu, Hailong Wang, Yue Xu, Yulong Chen, Xiaoliang Zhao, Jianquan Zhu, Zhenfa Zhang, Changli Wang
Summary: CITED4, upregulated in lung adenocarcinoma tissues and cells, is a predictor for disease-free survival. It plays a key role in invasion and metastasis of ADC, functioning synergistically with CTNNB1 to enhance CLDN3 transcription and induce ADC invasion via a CLDN3-dependent pathway.
Article
Cell Biology
Feifei Wang, Odjo G. Gouttia, Ling Wang, Aimin Peng
Summary: First-line treatments for oral cancer include surgery, radiation, and chemotherapy. However, tumor recurrence and acquired resistance remain major challenges. PARP1 upregulation in recurrent tumor cells was found, and PARP inhibitors were shown to partially reverse treatment resistance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Elise Rouleau-Turcotte, Dragomir B. Krastev, Stephen J. Pettitt, Christopher J. Lord, John M. Pascal
Summary: PARP1 can rapidly detect DNA strand break damage and activate the production of poly(ADP-ribose) by signaling break detection to its catalytic domain. This study provides insights into the contributions of the regulatory helical domain (HD) to PARP1 allostery and the interaction with DNA damage, as well as the mechanisms of PARP1 catalytic activation and retention on DNA damage.
Article
Multidisciplinary Sciences
Nicholas A. W. Bell, Justin E. Molloy
Summary: In this study, a new experimental method was developed to measure the mechanical stability and interaction kinetics of proteins across the two ends of a DNA double-strand break. It was found that PARP2 forms a stable mechanical link across blunt-end 5;- phosphorylated DSBs and restores torsional continuity. In contrast, PARP1 was not observed to form a bridging interaction across blunt or short overhang DSBs and competed away PARP2 bridge formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Shanshan Lin, Guihui Tu, Zelei Yu, Qingna Jiang, Lingyu Zhang, Jingwen Liu, Quanyu Liu, Xiuwang Huang, Jianhua Xu, Youwen Lin, Yang Liu, Lixian Wu
Summary: In this study, a series of compounds were synthesized using PROTAC technology to degrade PARP1 protein. CN0 compound was found to inhibit DNA damage repair, leading to the accumulation of DNA fragments and activation of immune pathways, ultimately enhancing the killing of tumor cells.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Zhong-Xuan Wang, Yi Liu, Yao-Lin Li, Qiao Wei, Rong-Rong Lin, Ruiqing Kang, Yang Ruan, Zhi-Hao Lin, Nai-Jia Xue, Bao-Rong Zhang, Jia-Li Pu
Summary: DNA damage and defective DNA repair are implicated in neurodegeneration of Parkinson's disease. The study shows that the protein DJ-1 plays a crucial role in modulating DNA double-strand break repair by promoting both homologous recombination and nonhomologous end joining. DJ-1 interacts with PARP1 and enhances its enzymatic activity during DNA repair. Importantly, PD patients with DJ-1 mutation have impaired PARP1 activity and DSB repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Evgeniia Prokhorova, Florian Zobel, Rebecca Smith, Siham Zentout, Ian Gibbs-Seymour, Kira Schutzenhofer, Alessandra Peters, Josephine Groslambert, Valentina Zorzini, Thomas Agnew, John Brognard, Michael L. Nielsen, Dragana Ahel, Sebastien Huet, Marcin J. Suskiewicz, Ivan Ahel
Summary: Recent findings suggest that serine ADP-ribosylation plays a vital role in cellular responses to PARP1/PARP2 inhibitors. The efficient modification of three serine residues within PARP1 by HPF1 counters PARP1 trapping and contributes to inhibitor tolerance, implicating these residues as potential biomarkers for PARP inhibitor therapy.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Yuechao Liu, Yang Song, Mengru Cao, Weina Fan, Yaowen Cui, Yimeng Cui, Yuning Zhan, Ruixue Gu, Fanglin Tian, Shuai Zhang, Li Cai, Ying Xing
Summary: This study identified endocytic and metastasis-associated genes (EMGs) and investigated their biological functions and mechanisms. EHD1 was found to play a significant role in suppressing the traits and processes associated with cancer metastasis. The study provides insights into the regulatory mechanisms involving EHD1 and its interaction with CD44 in lung adenocarcinoma.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biology
Alvaro Gonzalez Rajal, Kamila A. Marzec, Rachael A. McCloy, Max Nobis, Venessa Chin, Jordan F. Hastings, Kaitao Lai, Marina Kennerson, William E. Hughes, Vijesh Vaghjiani, Paul Timpson, Jason E. Cain, D. Neil Watkins, David R. Croucher, Andrew Burgess
Summary: The study identified a non-genetic mechanism of resistance in human lung adenocarcinoma that is dependent on the cell cycle stage at the time of cisplatin exposure, using RNA sequencing and biosensors to track single-cell fates.
Article
Cell Biology
Hanlin Ma, Gonghua Qi, Fang Han, Wei Lu, Jiali Peng, Rongrong Li, Shi Yan, Cunzhong Yuan, Beihua Kong
Summary: This study found that the overexpression of HMGB3 is associated with shorter overall survival and drug resistance in high-grade serous ovarian carcinoma. HMGB3 interacts with PARP1 to promote PARPi resistance, and targeted inhibition of HMGB3 may overcome PARPi resistance in ovarian cancer therapy.
CELL DEATH & DISEASE
(2022)
Article
Pharmacology & Pharmacy
Manni Wang, Siyuan Chen, Yuquan Wei, Xiawei Wei
Summary: The study identified that the DNA-PK inhibitor M3814 enhanced the anti-tumor effect of chemotherapy in NSCLC cell lines, providing a theoretical basis for its potential use in clinical practice to optimize NSCLC treatment.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Food Science & Technology
S. Ahmad, M. L. Tan, Shahrul Hamid
Summary: Cardiovascular disease is a leading global cause of death, influenced by factors such as genetics, high-fat intake, smoking, hypertension, diabetes, and lifestyle. The disease is caused by the sudden production of reactive oxygen species, resulting in DNA damage and cell death. Various forms of DNA damage have been associated with cardiovascular disease. Therefore, reducing reactive oxygen species is crucial, and nutraceuticals and their bioactive compounds play a role in mitigating DNA damage.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Cell Biology
Soyoung Chang, Pil Soo Sung, Jungsul Lee, Junseong Park, Eui-Cheol Shin, Chulhee Choi
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2016)
Article
Biochemistry & Molecular Biology
Zheng Li, Seung-Wook Ryu, Jungsul Lee, Kyungsun Choi, Sunchang Kim, Chulhee Choi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Oncology
Woo-Yong Park, Beom-Jin Hong, Jungsul Lee, Chulhee Choi, Mi-Young Kim
Article
Multidisciplinary Sciences
Nambin Yim, Seung-Wook Ryu, Kyungsun Choi, Kwang Ryeol Lee, Seunghee Lee, Hojun Choi, Jeongjin Kim, Mohammed R. Shaker, Woong Sun, Ji-Ho Park, Daesoo Kim, Won Do Heo, Chulhee Choi
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Jingang Hou, Sunchang Kim, Changkeun Sung, Chulhee Choi
Article
Multidisciplinary Sciences
Woogwang Sim, Jungsul Lee, Chulhee Choi
SCIENTIFIC REPORTS
(2017)
Article
Engineering, Biomedical
Yuri An, Yujung Kang, Jungsul Lee, Chulwoo Ahn, Kihwan Kwon, Chulhee Choi
BIOMEDICAL ENGINEERING ONLINE
(2018)
Article
Biochemistry & Molecular Biology
Jingang Hou, Changhao Cui, Sunchang Kim, Changkeun Sung, Chulhee Choi
CHEMICO-BIOLOGICAL INTERACTIONS
(2018)
Article
Cell Biology
Yieun Jung, So-Hee Ahn, Hyunju Park, Sang Hui Park, Kyungsun Choi, Chulhee Choi, Jihee Lee Kang, Youn-Hee Choi
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
(2018)
Article
Multidisciplinary Sciences
Jungwhoi Lee, Jungsul Lee, Jeong Hun Yun, Chulhee Choi, Sayeon Cho, Seung Jun Kim, Jae Hoon Kim
SCIENTIFIC REPORTS
(2017)
Article
Cell & Tissue Engineering
Gyuhyeon Baek, Hojun Choi, Youngeun Kim, Hai-Chon Lee, Chulhee Choi
STEM CELLS TRANSLATIONAL MEDICINE
(2019)
Article
Biochemical Research Methods
Jungsul Lee, Chulhee Choi
Article
Biotechnology & Applied Microbiology
Woogwang Sim, Junghwa Cha, Chulhee Choi, Kyungsun Choi
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2017)
Article
Oncology
Cheryl Wanderi, Eunsoo Kim, Soyoung Chang, Chulhee Choi, Kyungsun Choi
ANTICANCER RESEARCH
(2016)