Article
Biochemistry & Molecular Biology
Ruoxuan Sun, Seung-Oe Lim
Summary: This study reveals a novel mechanism for the regulation of 4-1BB at the protein level, involving polyubiquitination and degradation via the ubiquitin-proteasomal system. These findings may provide guidance for the development of targeted therapies for cancer and other immune disorders.
Article
Multidisciplinary Sciences
Hao Zhang, Zebin Deng, Yilong Wang, Xiaoping Zheng, Lizhi Zhou, Shu Yan, Yinhuai Wang, Yingbo Dai, Yashpal. S. Kanwar, Fei Deng
Summary: This study reveals that CHIP regulates cell pyroptosis in septic acute kidney injury by promoting the degradation of NLRP3. CHIP may serve as a potential therapeutic target for S-AKI.
Article
Endocrinology & Metabolism
Yuguo Niu, Haizhou Jiang, Hanrui Yin, Fenfen Wang, Ronggui Hu, Xiaoming Hu, Bo Peng, Yousheng Shu, Zhigang Li, Shanghai Chen, Feifan Guo
Summary: Chronic inflammation in the liver leads to insulin resistance in the body and skeletal muscle. This study identified ERAP1 as a hepatokine induced by chronic inflammation and showed that increased ERAP1 impairs insulin sensitivity in the skeletal muscle. ERAP1 acts as an antagonist-like factor by interacting with ADRB2 and interfering with insulin signaling. Inhibiting ERAP1 may provide a therapeutic strategy for insulin resistance-related diseases.
Article
Multidisciplinary Sciences
Larissa Dietz, Cara J. Ellison, Carlos Riechmann, C. Keith Cassidy, F. Daniel Felfoldi, Adan Pinto-Fernandez, Benedikt M. Kessler, Paul R. Elliott
Summary: Certain members of the inhibitor of apoptosis (IAP) family, including BIRC6, regulate cell death by inhibiting caspases, while SMAC antagonizes IAPs and promotes cell death. BIRC6 directly inhibits caspase-3 and-7 and ubiquitinates caspase-3,-7, and-9, working with noncanonical E1, UBA6. SMAC suppresses both mechanisms by competitively displacing caspases.
Article
Oncology
Rati Lama, Chao Xu, Samuel L. Galster, Javier Querol-Garcia, Scott Portwood, Cory K. Mavis, Federico M. Ruiz, Diana Martin, Jin Wu, Marianna C. Giorgi, Jill Bargonetti, Eunice S. Wang, Francisco J. Hernandez-Ilizaliturri, Gerald B. Koudelka, Sherry R. Chemler, Ines G. Munoz, Xinjiang Wang
Summary: This study identifies MMRi62 as a novel MDM2-MDM4-targeting agent that can induce apoptosis in leukemia cells, including those with non-functional p53.
FRONTIERS IN ONCOLOGY
(2022)
Article
Neurosciences
Songwang Xie, Zhen Hong, Yan Li, Junyong Wang, Jian Wang, Shaoquan Li, Yongchang Liu
Summary: The study found that RNF216 is overexpressed in GBM and is associated with a poor prognosis. Overexpression of RNF216 promotes GBM cell growth and inhibits apoptosis, while knockdown of RNF216 has the opposite effects. RNF216 overexpression also degrades the p53 protein and negatively regulates the p53 pathway.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Immunology
Jing Yang, Hanh Chi Do-Umehara, Qiao Zhang, Huashan Wang, Changchun Hou, Huali Dong, Edith A. Perez, Marc A. Sala, Kishore R. Anekalla, James M. Walter, Shuwen Liu, Richard G. Wunderink, G. R. Scott Budinger, Jing Liu
Summary: JNK2 plays a crucial role in regulating lung inflammation and injury during sepsis and acute lung injury. The upregulation of miR-221-5p targets JNK2 mRNA leading to downregulation of JNK2, thereby exacerbating lung inflammation and injury during sepsis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Ophthalmology
Tiantian Qi, Ruihua Jing, Bo Ma, Conghui Hu, Chan Wen, Yongping Shao, Cheng Pei
Summary: This study reveals that the downregulation of RNF157 contributes to age-related cataract by upregulating p53, suggesting that modulation of RNF157 expression may serve as a potential therapeutic strategy for cataracts.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yuchen Feng, Aileen R. Ariosa, Ying Yang, Zehan Hu, Joern Dengjel, Daniel J. Klionsky
Summary: Macroautophagy/autophagy is a crucial molecular process for maintaining cellular homeostasis, recycling cytoplasmic materials, and responding to stress conditions. The posttranslational modification of Atg9, including ubiquitination and proteasome-dependent degradation, regulates the level of autophagy in cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Beibei Fu, Xiaoyuan Lin, Shun Tan, Rui Zhang, Weiwei Xue, Haiwei Zhang, Shanfu Zhang, Qingting Zhao, Yu Wang, Kelly Feldman, Lei Shi, Shaolin Zhang, Weiqi Nian, Krishna Chaitanya Pavani, Zhifeng Li, Xingsheng Wang, Haibo Wu
Summary: The research reveals that the miR-342-3p/SOCS6 axis plays a crucial role in regulating anti-Mtb immunity and the balance between cell apoptosis and necrosis during Mtb infection, providing new insights for developing host-directed therapies targeting these pathways.
Article
Oncology
Yucheng Liu, Xinyan Wang, Wucheng Zhu, Zhongheng Sui, Xiangqing Wei, Yang Zhang, Jiansong Qi, Yanhong Xing, Wuyang Wang
Summary: This study reveals the mechanism by which TRPML1 inhibits autophagy to regulate apoptosis, involving disrupted mitochondrial turnover, elevated ROS, DNA damage, and p53 activation. These findings have potential clinical implications for treating melanoma and glioblastoma.
Article
Biochemistry & Molecular Biology
John E. La Marca, Brandon J. Aubrey, Bruce Yang, Catherine Chang, Zilu Wang, Andrew Kueh, Lin Tai, Stephen Wilcox, Liz Milla, Susanne Heinzel, David Vremec, Lauren Whelan, Christina Konig, Deeksha Kaloni, Anne K. Voss, Andreas Strasser, Sarah T. Diepstraten, Marco J. Herold, Gemma L. Kelly
Summary: Whole-genome screens using CRISPR technologies can identify novel tumour suppressors and factors affecting cancer cell response to anti-cancer drugs. In this study, a genome-wide screen on lymphoma cells found that the absence of ARRDC3 enhances the survival and competitiveness of MYC-driven lymphoma cells treated with TRP53-activating anti-cancer drugs. Deleting Arrdc3 in mice resulted in perinatal lethality and cardiac defects. The absence of ARRDC3 significantly accelerated MYC-driven lymphoma development, highlighting its role as a new mediator of TRP53-mediated tumour suppression.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Cell Biology
Ying Liu, Hanwen Zhang, Yiwei Liu, Siyue Zhang, Peng Su, Lijuan Wang, Yaming Li, Yiran Liang, Xiaolong Wang, Weijing Zhao, Bing Chen, Dan Luo, Ning Zhang, Qifeng Yang
Summary: Mitophagy plays a crucial role in maintaining cellular homeostasis under stress conditions such as hypoxia. Dysregulation of mitophagy has been linked to disorders including neurodegenerative disease and cancer. This study identifies GPCPD1 as a key mediator of hypoxia-induced mitophagy in triple-negative breast cancer. GPCPD1 is depalmitoylated by LYPLA1, facilitating its relocation to the outer mitochondrial membrane and subsequent binding to VDAC1. The increased monomer of VDAC1 recruits PRKN-mediated polyubiquitination and triggers mitophagy. GPCPD1-mediated mitophagy promotes tumor growth and metastasis in triple-negative breast cancer. GPCPD1 could serve as an independent prognostic indicator in triple-negative breast cancer and a potential target for therapeutic development.
Article
Biochemistry & Molecular Biology
Yiming Tu, Lei Xu, Jia Xu, Zhongyuan Bao, Wei Tian, Yangfan Ye, Guangchi Sun, Zong Miao, Honglu Chao, Yongping You, Ning Liu, Jing Ji
Summary: This study identified USP2 as a new candidate gene that inhibits the development of GBM by deubiquitinating SMAD7 and suppressing the TGF-beta signaling pathway.
Article
Biology
Honglian Zhang, Ying Liu, Yali Xie, Yunji Zhu, Jingwen Liu, Falong Lu
Summary: DNA methylation and H3K27me3 are two chromatin modifications, and it is found that H3K27me3 can negatively regulate DNA methylation by inhibiting the methylation process. Loss of H3K27me3 leads to increased methylation at previously marked H3K27me3 sites, and genome-wide analysis shows the absence of H3 ubiquitination at H3K27me3 marked nucleosomes. In addition, loss of H3K27me3 modification induces an increase in H3K18 ubiquitination at the corresponding hyper-methylated loci. Importantly, it is demonstrated that H3K27me3 directly inhibits UHRF1-mediated H3 ubiquitination on nucleosomes.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Virology
Geng Yang, Jiajia Wang, Tao Luo, Xiaobo Zhang
Article
Biochemistry & Molecular Biology
Siyuan Zhang, Xiaobo Zhang
Article
Fisheries
Yuechao Sun, Xiaobo Zhang
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Yuechao Sun, Yu Zhang, Xiaobo Zhang
Article
Cell & Tissue Engineering
Fan Yang, Pei Cui, Yu Lu, Xiaobo Zhang
STEM CELL RESEARCH & THERAPY
(2019)
Article
Oncology
Yi Gong, Geng Yang, Qizhi Wang, Yumeng Wang, Xiaobo Zhang
MOLECULAR CANCER RESEARCH
(2020)
Article
Medicine, Research & Experimental
Song Zhang, Cuilian Liu, Xiaobo Zhang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2019)
Article
Cell & Tissue Engineering
Wenlin Wu, Chenxi Xu, Xiaobo Zhang, An Yu, Le Shu
STEM CELL RESEARCH & THERAPY
(2020)
Article
Microbiology
Xumei Sun, Junyi Yang, Minhui Zheng, Xiaobo Zhang
Summary: The study developed a biocoenosis generator that continuously released methane on the 1000-m deep-sea floor of the South China Sea, artificially constructing a deep-sea ecosystem biocoenosis. It was found that deep-sea biocoenosis consisted of anaerobic methane-oxidizing archaea, symbiotic archaea, bacteria, and animals, with viruses coexisting during the origin of the ecosystem.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Yalei Cui, Haitao Wan, Xiaobo Zhang
Summary: The study identified shrimp miR-34 as a potential antiviral miRNA against WSSV infection and suppressor of cancer progression, suggesting that animal antiviral miRNAs may have anti-tumor activity in humans.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Cell Biology
Chenxi Xu, Jianjian Zhuang, Xiaobo Zhang
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Siyuan Zhang, Fan Xin, Xiaobo Zhang
Summary: Compounds packaged in virions of white spot syndrome virus can trigger the viral life cycle by enhancing host glycolysis, leading to virus infection. This process involves the binding of palmitic amide to triosephosphate isomerase and the upregulation of HIF-1, enhancing glycolysis and promoting virus infection.
Review
Fisheries
Yi Gong, Xiaobo Zhang
Summary: Shrimp have developed innate immunity to fight against more than 20 viruses, with RNA interference (RNAi) playing a key role. Short interfering RNAs (siRNAs) and microRNAs (miRNAs) are important in virus-host interactions. The non-seed sequence in miRNAs is essential for their targeting function in regulating virus-host interactions.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Mengqi Chu, Haitao Wan, Xiaobo Zhang
Summary: The study revealed that hnRNP A2B1 is significantly upregulated in melanoma stem cells, playing an important role in promoting the expression of anti-apoptosis genes and suppressing pro-apoptosis genes by regulating their splicing. This ultimately leads to cell cycle arrest in G2 phase and apoptosis of melanoma stem cells, highlighting the crucial requirement of hnRNP A2B1 in tumorigenesis and providing novel insights into the role of hnRNPs in cancer stem cells.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Chemistry, Analytical
Jianjian Zhuang, Haitao Wan, Xiaobo Zhang
Summary: A biosensor using AuNP-modified AuE and CPs was developed for the detection of miR-100 in GC patients' sera. The biosensor showed high specificity and sensitivity in distinguishing miR-100 variants, with a detection range from 100 aM to 10 pM and a LOD of 100 aM. The cutoff for gastric cancer detection was determined to be 5 fM based on testing 100 human sera samples.