期刊
NUCLEIC ACIDS RESEARCH
卷 44, 期 9, 页码 4037-4051出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkw250
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资金
- NovoNordisk Foundation
- Danish Research Council for Strategic Research
- Lundbeck Foundation
- NovoNordisk Foundation centre grant (The Novo Nordisk Foundation section for Stem Cell biology in Human Disease)
- Lundbeck Foundation [R108-2012-10312] Funding Source: researchfish
- Novo Nordisk Foundation Section for Basic Stem Cell Biology [Porse group] Funding Source: researchfish
Formation of nucleosome free region (NFR) accompanied by specific histone modifications at flanking nucleosomes is an important prerequisite for enhancer and promoter activity. Due to this process, active regulatory elements often exhibit a distinct shape of histone signal in the form of a peak-valley-peak (PVP) pattern. However, different features of PVP patterns and their robustness in predicting active regulatory elements have never been systematically analyzed. Here, we present PARE, a novel computational method that systematically analyzes the H3K4me1 or H3K4me3 PVP patterns to predict NFRs. We show that NFRs predicted by H3K4me1 and me3 patterns are associated with active enhancers and promoters, respectively. Furthermore, asymmetry in the height of peaks flanking the central valley can predict the directionality of stable transcription at promoters. Using PARE on ChIP-seq histone modifications from four ENCODE cell lines and four hematopoietic differentiation stages, we identified several enhancers whose regulatory activity is stage specific and correlates positively with the expression of proximal genes in a particular stage. In conclusion, our results demonstrate that PVP patterns delineate both the histone modification landscape and the transcriptional activities governed by active enhancers and promoters, and therefore can be used for their prediction. PARE is freely available at http://servers.binf.ku.dk/pare.
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