Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes inEscherichia coli

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis inEscherichia coli

(2016) Bradley Walters Biggs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation

(2016) Iman Farasat et al.   PLoS Computational Biology

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways

(2015) Brady F Cress et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic pathway balancing and its role in the production of biofuels and chemicals

(2015) J Andrew Jones et al.   CURRENT OPINION IN BIOTECHNOLOGY

Versatile genetic assembly system (VEGAS) to assemble pathways for expression inS. cerevisiae

(2015) Leslie A. Mitchell et al.   NUCLEIC ACIDS RESEARCH

YeastFab: the design and construction of standard biological parts for metabolic engineering inSaccharomyces cerevisiae

(2015) Yakun Guo et al.   NUCLEIC ACIDS RESEARCH

Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage

(2015) Eric A. Josephs et al.   NUCLEIC ACIDS RESEARCH

A Cas9-guide RNA complex preorganized for target DNA recognition

(2015) F. Jiang et al.   SCIENCE

ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways

(2015) J. Andrew Jones et al.   Scientific Reports

Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9

(2015) Andriy Didovyk et al.   ACS Synthetic Biology

CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli

(2015) Brady F. Cress et al.   ACS Synthetic Biology

The Transcription Factor Titration Effect Dictates Level of Gene Expression

(2014) Robert C. Brewster et al.   CELL

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase

(2014) T. H. Segall-Shapiro et al.   Molecular Systems Biology

Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks

(2014) A. A. Nielsen et al.   Molecular Systems Biology

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression

(2014) Michelle L. Luo et al.   NUCLEIC ACIDS RESEARCH

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

(2014) Y. Lin et al.   NUCLEIC ACIDS RESEARCH

Planting the seed: target recognition of short guide RNAs

(2014) Tim Künne et al.   TRENDS IN MICROBIOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Advances in genetic circuit design: novel biochemistries, deep part mining, and precision gene expression

(2013) Alec AK Nielsen et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Genomic mining of prokaryotic repressors for orthogonal logic gates

(2013) Brynne C Stanton et al.   Nature Chemical Biology

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly

(2013) Joseph P. Torella et al.   NUCLEIC ACIDS RESEARCH

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

Folding RNA/DNA hybrid duplexes

(2012) Ronny Lorenz et al.   BIOINFORMATICS

A novel reporter system for bacterial and mammalian cells based on the non-ribosomal peptide indigoidine

(2012) Marius Müller et al.   METABOLIC ENGINEERING

A transcription activator-like effector toolbox for genome engineering

(2012) Neville E Sanjana et al.   Nature Protocols

A tunable zinc finger-based framework for Boolean logic computation in mammalian cells

(2012) Jason J. Lohmueller et al.   NUCLEIC ACIDS RESEARCH

Engineering synthetic TAL effectors with orthogonal target sites

(2012) Abhishek Garg et al.   NUCLEIC ACIDS RESEARCH

Modular control of multiple pathways using engineered orthogonal T7 polymerases

(2012) Karsten Temme et al.   NUCLEIC ACIDS RESEARCH

Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology

(2012) Benjamin A. Blount et al.   PLoS One

ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli

(2012) Peng Xu et al.   ACS Synthetic Biology

Thermodynamic contributions of single internal rA·dA, rC·dC, rG·dG and rU·dT mismatches in RNA/DNA duplexes

(2010) Norman E. Watkins et al.   NUCLEIC ACIDS RESEARCH