Article
Biochemistry & Molecular Biology
Stefan J. Kaliszuk, Natasha I. Morgan, Taylor N. Ayers, Courtney E. Sparacino-Watkins, Anthony W. DeMartino, Kaitlin Bocian, Venkata Ragireddy, Qin Tong, Jests Tejero
Summary: Cytoglobin is a hemoprotein that can reduce nitrite to nitric oxide and bind lipids. Mutations of specific amino acids such as His81 (E7), Arg84, and Lys116 have implications on the reactivity towards nitrite, affinity for lipids, and lipid binding stability. These gain and loss-of-function mutations can be useful tools to study the physiological role of cytoglobin functions in vivo.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nanako Yano, Toshifumi Minamoto, Hirosi Yamaguchi, Toshiyuki Goto, Takahito Nishikata
Summary: By studying the expression patterns of Ciona globin genes, it was found that two genes are similar to vertebrate hemoglobin-alpha and -beta, and are predominantly expressed in specific organs and induced under hypoxia. However, there was no robust similarity between the other Ciona globins and vertebrate globins. These results suggest that there is some physicochemical and physiological similarity between Ciona globins and vertebrate globins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
John Ukeri, Michael T. Wilson, Brandon J. Reeder
Summary: Cytoglobin has physiological roles that are not clearly understood, but may include regulation of nitric oxide, oxygen sensing, and protection against oxidative stress. Specific mutations can affect the nitric oxide binding and metabolism activities of cytoglobin, helping to understand its precise role under physiological and pathophysiological conditions.
Review
Biochemistry & Molecular Biology
Marina A. Semenova, Rita V. Chertkova, Mikhail P. Kirpichnikov, Dmitry A. Dolgikh
Summary: Neuroglobin, a heme protein predominantly expressed in nervous tissue, has been shown to promote neuronal survival. However, the molecular mechanisms underlying its neuroprotective function remain unclear. Recent experimental and computational work suggests that the interaction between neuroglobin and mitochondrial cytochrome c may contribute to neuroglobin-mediated neuroprotection, both in the cytoplasm and within the mitochondria.
Article
Biochemistry & Molecular Biology
Yan-Yan Li, Shuang-Shuang Long, Lu Yu, Ao-Kun Liu, Shu-Qin Gao, Xiangshi Tan, Ying-Wu Lin
Summary: The naturally occurring S47F/A mutations in hCyt c enhanced its peroxidase activity and reduced its binding affinity with hNgb. These findings provide valuable insights into the role of Ser47 in sustaining the structure and function of hCyt c.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Cecile Exertier, Federico Sebastiani, Ida Freda, Elena Gugole, Gabriele Cerutti, Giacomo Parisi, Linda Celeste Montemiglio, Maurizio Becucci, Cristiano Viappiani, Stefano Bruno, Carmelinda Savino, Carlotta Zamparelli, Massimiliano Anselmi, Stefania Abbruzzetti, Giulietta Smulevich, Beatrice Vallone
Summary: We produced a variant of neuroglobin, named Ngb CDless, and investigated the role of the CDloop-D-helix unit in its structure dynamics and ligand binding. The results showed that Ngb CDless retains ligand binding ability while abolishing the structure dynamics of CDloop-D-helix.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joey De Backer, Darko Maric, Karim Zuhra, Annemie Bogaerts, Csaba Szabo, Wim Vanden Berghe, David Hoogewijs
Summary: This study investigated the role of CYGB in melanoma cells. The findings suggest that CYGB knockdown increases the sensitivity of cells to ferroptosis and pyroptosis cell death signaling pathways, supporting a tumor-suppressive role for CYGB and its modulation of cancer therapy sensitivity.
Article
Reproductive Biology
Derya Sagrac, Selinay Senkal, Taha Bartu Hayal, Selami Demirci, Hatice Burcu Sisli, Ayla Burcin Asutay, Aysegul Dogan
Summary: CYGB and NGB proteins play an important role in protecting Leydig cells from dysfunction under inflammatory and oxidative stress conditions. Downregulation of CYGB and NGB leads to increased release of nitride oxide, blocked cell cycle progression, reduced testosterone production, and increased expression of inflammatory and apoptotic pathway genes. On the other hand, overexpression of CYGB and NGB reduces the expression of inflammatory proteins, increases the expression of testosterone biogenesis pathway genes, and improves testosterone production.
REPRODUCTIVE BIOLOGY
(2022)
Review
Electrochemistry
Xingmei Lu, Haoqiang Song, Jinmeng Cai, Siyu Lu
Summary: This review focuses on the latest research progress in the field of electrochemical nitrate reduction reaction to ammonia, discussing the mechanism, catalysts, and detection methods, and exploring the future challenges and prospects.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Inorganic & Nuclear
Yan Zhou, Yunlong Meng, Xingzhao Wang, Jiabing Luo, Hanhan Xia, Wenle Li, Jun Zhang
Summary: Improving the performance of CuO in electrocatalytic nitrite reduction to ammonia (NIRA) is the primary goal. This is achieved by growing Co3O4 nanospheres on CuO, enhancing its electrocatalytic activity. Comparing Co3O4@CuO with mechanically mixed CuO and Co3O4 on a rotating ring-disk electrode, it was found that the enhancement was due to a dual-site catalytic pathway.
DALTON TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Valerio G. Giacobelli, Kosuke Fujishima, Martin Lepsik, Vyacheslav Tretyachenko, Tereza Kadava, Mikhail Makarov, Lucie Bednarova, Petr Novak, Klara Hlouchova
Summary: RNA-peptide/protein interactions are crucial to life and have been studied in the context of early evolution. This study focused on an RNA-binding variant of the ribosomal uL11 C-terminal domain, selected from a library of prebiotically plausible amino acid sequences. The study found that the selected variant binds RNA with a similar overall affinity, utilizing ion bridging interactions for stabilization in the absence of aromatic/basic residues. These findings provide insights into how early protein-RNA interactions may have evolved.
MOLECULAR BIOLOGY AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Suresh Velnati, Sara Centonze, Federico Girivetto, Daniela Capello, Ricardo M. Biondi, Alessandra Bertoni, Roberto Cantello, Beatrice Ragnoli, Mario Malerba, Andrea Graziani, Gianluca Baldanzi
Summary: PKC zeta and PKC iota/lambda form the atypical protein kinase C subgroup, characterized by their regulation distinct from conventional PKCs. Both PKC zeta and PKC iota interact with phosphatidic acid and phosphatidylserine, while PKC iota uniquely binds to phosphatidylinositol-monophosphates. Additionally, phosphatidylinositol 4-phosphate specifically activates PKC iota, indicating a specific regulation of PKC iota by certain membrane lipids.
Article
Genetics & Heredity
Daniela Giordano, Paola Corti, Daniela Coppola, Giovanna Altomonte, Jianmin Xue, Roberta Russo, Guido di Prisco, Cinzia Verde
Summary: Antarctic fish species, particularly the icefish, have unique adaptations to cope with cold environments but the regulatory mechanisms for temperature and oxygen-induced gene expression changes are still largely unknown. Proteins such as globins play crucial roles in mediating Antarctic fish adaptations to environmental changes.
Review
Chemistry, Inorganic & Nuclear
Wei Song, Luchao Yue, Xiaoya Fan, Yongsong Luo, Binwu Ying, Shengjun Sun, Dongdong Zheng, Qian Liu, Mohamed S. Hamdy, Xuping Sun
Summary: This review discusses the reaction mechanism of the electrochemical reduction of NO3- and systematically summarizes recent developments in electrocatalysts for the NO3-RR. Various design strategies to enhance the performance of NO3-RR, such as defect engineering, rational structure design, strain engineering, and constructing heterostructures, are discussed. The authors also illustrate how understanding these optimization strategies can provide fundamental insights into the yield rate, faradaic efficiency, and selectivity of electrocatalysts for NH3 synthesis.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Physical
Jeonghoon Lim, Yu Chen, David A. Cullen, Seung Woo Lee, Thomas P. Senftle, Marta C. Hatzell
Summary: By controlling the copper surface coverage, palladium nanocubes as electrocatalysts can effectively reduce nitrate (NO3-) and increase the selectivity for the reduction of nitrite (NO2-) to N2 or NH4+. Partial copper-coated Pd nanocubes can reduce 95% of NO3- and have 89% selectivity for NO2- reduction over 20 cycles (80 h). Complete copper-covered Pd nanocubes can reduce about 99% of NO3- and selectively reduce NO2- to NH4+ with a 70% selectivity over 20 cycles (80 h).
Article
Biochemistry & Molecular Biology
Elizabeth R. Rochon, Maria Azzurra Missinato, Jianmin Xue, Jesus Tejero, Michael Tsang, Mark T. Gladwin, Paola Corti
ANTIOXIDANTS & REDOX SIGNALING
(2020)
Article
Biochemistry & Molecular Biology
Jesus Tejero
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Pharmacology & Pharmacy
Ling Wang, Courtney E. Sparacino-Watkins, Jun Wang, Nadeem Wajih, Paul Varano, Qinzi Xu, Eric Cecco, Jesus Tejero, Manoocher Soleimani, Daniel B. Kim-Shapiro, Mark T. Gladwin
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jason J. Rose, Kaitlin A. Bocian, Qinzi Xu, Ling Wang, Anthony W. DeMartino, Xiukai Chen, Catherine G. Corey, Danielle A. Guimaraes, Ivan Azarov, Xueyin N. Huang, Qin Tong, Lanping Guo, Mehdi Nouraie, Charles F. McTiernan, Christopher P. O'Donnell, Jesus Tejero, Sruti Shiva, Mark T. Gladwin
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Hematology
Anthony W. DeMartino, Jason J. Rose, Matthew B. Amdahl, Matthew R. Dent, Faraaz A. Shah, William Bain, Bryan J. McVerry, Georgios D. Kitsios, Jesus Tejero, Mark T. Gladwin
Article
Medicine, Research & Experimental
Tolani F. Olonisakin, Tomeka Suber, Shekina Gonzalez-Ferrer, Zeyu Xiong, Hernan F. Penaloza, Rick van der Geest, Yuting Xiong, David O. Osei-Hwedieh, Jesus Tejero, Matthew R. Rosengart, Wendy M. Mars, Daria Van Tyne, Andreas Perlegas, Samuel Brashears, Daniel B. Kim-Shapiro, Mark T. Gladwin, Michael A. Bachman, Eldad A. Hod, Claudette St Croix, Yulia Y. Tyurina, Valerian E. Kagan, Rama K. Mallampalli, Anuradha Ray, Prabir Ray, Janet S. Lee
Summary: Macrophages play a crucial role in heme metabolism and immune defense, but excessive heme can lead to immunosuppression. The clearance of senescent RBCs by macrophages impairs immunity and is independent of bacterial iron acquisition.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Hematology
Ravi Vats, Tomasz W. Kaminski, Eun-Mi Ju, Tomasz Brozska, Egemen Tutuncuoglu, Jesus Tejero, Enrico M. Novelli, Prithu Sundd, Tirthadipa Pradhan-Sundd
Summary: The chronic deficiency of P-selectin attenuates liver ischemia but fails to prevent hepatobiliary injury in SCD mice, possibly due to an increase in cellular senescence and reduced epithelial cell proliferation in the liver. This highlights the importance of investigating the long-term effects of chronic P-selectin inhibition therapy on liver pathophysiology in patients with SCD.
Article
Cardiac & Cardiovascular Systems
Jeffrey Wang, Willard N. Applefeld, Junfeng Sun, Steve B. Solomon, Jing Feng, Zoe G. Couse, Thomas F. Risoleo, Robert L. Danner, Jesus Tejero, Juan Lertora, Elmira Alipour, Swati Basu, Vandana Sachdev, Daniel B. Kim-Shapiro, Mark T. Gladwin, Harvey G. Klein, Charles Natanson
Summary: The study found that elevated CFH levels in septic shock lead to various pathophysiological changes, including increased pulmonary arterial pressures and right ventricular afterload, depressed cardiac function, worsening shock, lactate levels, metabolic acidosis, and multiorgan failure. These effects are mainly attributed to nitric oxide scavenging and iron supply promoting bacterial growth.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Matthew R. Dent, Anthony W. DeMartino, Jesus Tejero, Mark T. Gladwin
Summary: Interdisciplinary research has revealed the essential roles of nitric oxide (NO) as a signaling molecule in various physiological pathways relevant to human health and disease. Hemeproteins, including hemoglobin and myoglobin, play central roles in generating and maintaining NO as well as serving as a stable reservoir for NO in the form of nitrite. These pathways involve complex chemical and biochemical reactions that govern NO and nitrite signaling in vivo, presenting potential therapeutic targets for treating a wide range of diseases.
INORGANIC CHEMISTRY
(2021)
Article
Hematology
Ravi Vats, Tomasz W. Kaminski, Tomasz Brzoska, John A. Leech, Egemen Tutuncuoglu, Omika Katoch, Jude Jonassaint, Jesus Tejero, Enrico M. Novelli, Tirthadipa Pradhan-Sundd, Mark T. Gladwin, Prithu Sundd
Summary: This study reveals for the first time that the sterile inflammatory milieu in SCD promotes caspase-4/11-dependent activation of GSDMD, and facilitates the liver-to-lung embolic translocation of NETs, leading to the development of acute lung injury.
Article
Biochemistry & Molecular Biology
Stefan J. Kaliszuk, Natasha I. Morgan, Taylor N. Ayers, Courtney E. Sparacino-Watkins, Anthony W. DeMartino, Kaitlin Bocian, Venkata Ragireddy, Qin Tong, Jests Tejero
Summary: Cytoglobin is a hemoprotein that can reduce nitrite to nitric oxide and bind lipids. Mutations of specific amino acids such as His81 (E7), Arg84, and Lys116 have implications on the reactivity towards nitrite, affinity for lipids, and lipid binding stability. These gain and loss-of-function mutations can be useful tools to study the physiological role of cytoglobin functions in vivo.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Qinzi Xu, Jason J. Rose, Xiukai Chen, Ling Wang, Anthony W. DeMartino, Matthew R. Dent, Sagarika Tiwari, Kaitlin Bocian, Xueyin N. Huang, Qin Tong, Charles F. McTiernan, Lanping Guo, Elmira Alipour, Trevor C. Jones, K. Burak Ucer, Daniel B. Kim-Shapiro, Jesus Tejero, Mark T. Gladwin
Summary: This study evaluates different hemoproteins and finds that 2,3-diphosphoglycerate stripped human hemoglobin (StHb) and N-ethylmaleimide modified hemoglobin (NEMHb) have high CO binding affinities and efficiently scavenge CO from erythrocytes, suggesting their potential therapeutic role in CO poisoning.
Article
Biochemistry & Molecular Biology
Anthony W. DeMartino, Laxman Poudel, Matthew R. Dent, Xiukai Chen, Qinzi Xu, Brendan S. Gladwin, Jesus Tejero, Swati Basu, Elmira Alipour, Yiyang Jiang, Jason J. Rose, Mark T. Gladwin, Daniel B. Kim-Shapiro
Summary: Nitric oxide (NO) is a signaling molecule that plays a role in regulating blood flow and platelet activation. However, the diffusion of NO is limited by reactions with hemoproteins. This study suggests that NO can be stabilized as NO-ferroheme and transported within the body to promote vasodilation.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anthony W. DeMartino, Matthew B. Amdahl, Kaitlin Bocian, Jason J. Rose, Jesus Tejero, Mark T. Gladwin
Summary: The research indicates that the His81 binding equilibrium of cytoglobin is modulated by the formation of a disulfide bridge between Cys38 and Cys83. Experimental results show the presence of at least two kinetically distinct subpopulations in cytoglobin, depending on the thiol oxidation states.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Anthony DeMartino, Jesus Tejero, Mark Gladwin
FREE RADICAL BIOLOGY AND MEDICINE
(2020)