Article
Biochemistry & Molecular Biology
Xingxing Wang, Wenjing Sun, Shiyuan Fang, Boran Dong, JinXing Li, Zongyou Lv, Wankui Li, Wansheng Chen
Summary: Artemisinin, extracted from Artemisia annua L., is an important drug for malaria treatment and has potential applications in other diseases. The regulatory mechanism of artemisinin production is still not well understood. In this study, WRKY6 was identified as a key transcription factor that binds to the promoter of the artemisinin biosynthesis gene DBR2, thereby regulating artemisinin synthesis and content during the growth cycle of A. annua.
Article
Plant Sciences
Danial Hassani, Ayat Taheri, Xueqing Fu, Wei Qin, Liu Hang, Yanan Ma, Kexuan Tang
Summary: The study focused on increasing the artemisinin content in Artemisia annua plants through genetic engineering. By overexpressing three artemisinin biosynthesis enzymes and two transcription factors, the content of artemisinin in the transgenic plants was significantly increased in both T0 and T1 generations.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zongyou Lv, JinXing Li, Shi Qiu, Fei Qi, Hang Su, Qitao Bu, Rui Jiang, Kexuan Tang, Lei Zhang, Wansheng Chen
Summary: This study reveals that the transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin content in Artemisia annua by lowering gibberellin levels, which may provide a means to enhance artemisinin yields.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Shuwei Chang, Qi Li, Baokang Huang, Wansheng Chen, Hexin Tan
Summary: This study systematically identified and characterized 226 putative bHLH transcription factors in A. annua. These transcription factors play important roles in regulating key biological processes such as artemisinin biosynthesis and trichome development.
Article
Plant Sciences
Yongpeng Li, Wei Qin, Xueqing Fu, Yaojie Zhang, Danial Hassani, Sadaf-Ilyas Kayani, Lihui Xie, Hang Liu, Tiantian Chen, Xin Yan, Bowen Peng, Kuanyu Wu-Zhang, Chen Wang, Xiaofen Sun, Ling Li, Kexuan Tang
Summary: UV-B radiation plays a pivotal role in regulating plant growth and metabolite biosynthesis. In Artemisia annua, the transcriptional responses to UV-B radiation revealed potential involvement of specific TFs, such as AaMYB6 and AaMYB4, in the regulation of artemisinin and flavonoid biosynthesis pathways.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Sadaf-Ilyas Kayani, Yanan Ma, Xueqing Fu, Qian Shen, Yongpeng Li, Saeed-ur Rahman, Bowen Peng, Liu Huang, Kexuan Tang
Summary: This study reveals the interaction between AaYABBY5 and AaWRKY9, which indirectly regulates artemisinin production. AaYABBY5 promotes the activity of AaGSW1 and AaDBR2, while AaJAZ8 inhibits the activity of AaYABBY5. This study provides the molecular basis for the regulation of artemisinin biosynthesis through YABBY-WRKY interactions and highlights AaYABBY5 overexpression plants as a valuable genetic resource.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jiao Li, Xiao-Hui Chu, Xiao-Yu Wang, Bao-Min Feng, Zong-Xia Yu
Summary: Research shows that as the plant ages, artemisinin (ART) gradually accumulates in the leaves of Artemisia annua. Analysis at the sRNA and transcriptome levels indicates that age plays a significant regulatory role in the biosynthesis of ART.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Environmental
Kaiser Iqbal Wani, M. Naeem, M. Masroor A. Khan, Tariq Aftab
Summary: This study investigates the role of strigolactones (SLs) in alleviating cadmium (Cd) toxicity in Artemisia annua plants. The results show that SLs improve the photosynthetic performance and Cd tolerance by modulating the antioxidant enzyme system, protecting chloroplasts and pigments, and enhancing glandular trichome (GT) attributes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Limeng Zhou, Yingzhang Huang, Qi Wang, Dianjing Guo
Summary: Our study identified high and low accessible regions in glandular trichomes, suggesting their contribution to specific biological functions. Certain artemisinin biosynthetic genes in glandular trichomes showed higher accessible regions compared to leaf, indicating potential regulation by chromatin accessibility. Transcription factor binding motifs for MYB, bZIP, C2H2, and AP2 were overrepresented in highly accessible chromatin regions associated with artemisinin biosynthetic genes.
Article
Pharmacology & Pharmacy
Guoping Shu, Yueli Tang, Mingyuan Yuan, Ning Wei, Fangyuan Zhang, Chunxian Yang, Xiaozhong Lan, Min Chen, Kexuan Tang, Lien Xiang, Zhihua Liao
Summary: The AabZIP1-AaMYC2 transcriptional module is revealed as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis, which may have general implications.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Plant Sciences
Hang Liu, Ling Li, Xueqing Fu, Yongpeng Li, Tiantian Chen, Wei Qin, Xin Yan, Zhangkuanyu Wu, Lihui Xie, Sadaf-llyas Kayani, Danial Hassani, Xiaofen Sun, Kexuan Tang
Summary: In this study, a transcription factor named AaMYB108 was identified as a positive regulator of artemisinin biosynthesis in A. annua. AaMYB108 promotes artemisinin biosynthesis by interacting with a previously characterized positive regulator, AaGSW1. Additionally, AaMYB108 interacts with AaCOP1 and AaJAZ8, and its function is influenced by these proteins. Through experiments with transgenic plants, it was found that the promotion of artemisinin by light and JA depends on the presence of AaMYB108. Overall, this study reveals the molecular mechanism of JA regulating artemisinin biosynthesis depending on light and provides new insights into the integration of light and phytohormone signaling in plants.
Article
Plant Sciences
Tian-Tian Chen, Xing-Hao Yao, Hang Liu, Yong-Peng Li, Wei Qin, Xin Yan, Xiu-Yun Wang, Bo-Wen Peng, Yao-Jie Zhang, Jin Shao, Xin-Yi Hu, Qing Miao, Xue-Qing Fu, Yu-Liang Wang, Ling Li, Ke-Xuan Tang
Summary: A MADS transcription factor, AaSEP4, has been identified as a positive regulator of artemisinin biosynthesis in Artemisia annua. AaSEP4 activates the expression of GLANDULAR TRICHOME-SPECIFIC WRKY1 and directly binds to its promoter region. Overexpression of AaSEP4 significantly increases artemisinin content in A. annua, making it a potential target for enhancing artemisinin production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Jose Abramo Marchese, Jorge F. S. Ferreira, Rita Maria Moraes, Franck E. Dayan, Michelle F. F. Rodrigues, Jorge Jamhour, Lucas Vinicius Dallacorte
Summary: In this study, a phenological scale was established for Artemisia annua L. to facilitate successful cross-pollination and breeding. The scale divided the plant's growth cycle into the vegetative phase and reproductive phase, with distinct stages identified through daily observations and photographs. The experiments showed that genotypes from different geographic origins demonstrate better synchronization under both short days and low temperatures, and the concentration and yield of artemisinin vary depending on the photoperiod and growth phase.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Boran Dong, Xingxing Wang, Rui Jiang, Shiyuan Fang, Jinxing Li, Qing Li, Zong You Lv, Wan Sheng Chen
Summary: In this study, AaCycTL was found to regulate trichome development and affect the content of artemisinin in Artemisia annua. Overexpression of AaCycTL in Arabidopsis and A. annua resulted in changes in wax coverage on the plant surface and decreased trichome density. The interaction between AaCycTL and AaTAR1 was identified, indicating a potential role of AaCycTL in regulating trichome density and artemisinin biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yongpeng Li, Tiantian Chen, Hang Liu, Wei Qin, Xin Yan, Kuanyu Wu-Zhang, Bowen Peng, Yaojie Zhang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: A new promoter, tpACT, was discovered in this study, showing higher activity than the commonly used CaMV35S promoter in artemisinin-producing plants. Application of tpACT in genetic transformation of Artemisia annua can increase the production of artemisinin.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zongyou Lv, JinXing Li, Shi Qiu, Fei Qi, Hang Su, Qitao Bu, Rui Jiang, Kexuan Tang, Lei Zhang, Wansheng Chen
Summary: This study reveals that the transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin content in Artemisia annua by lowering gibberellin levels, which may provide a means to enhance artemisinin yields.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
S. Ochatt, A. R. Alan, A. Bhattacharya, C. Hano, K. Kiselev, P. L. Marconi, W. C. Otoni, S. Y. Park, K. X. Tang, P. J. Weathers
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Yilong He, Xueqing Fu, Ling Li, Xiaofen Sun, Kexuan Tang, Jingya Zhao
Summary: In this study, AaSPL9 was identified as a positive regulator of glandular trichome initiation in Artemisia annua. Overexpression of AaSPL9 increased the density of glandular trichomes and the content of artemisinin. The study also revealed the molecular mechanism by which AaSPL9 promotes glandular trichome initiation by activating the expression of AaHD1.
Article
Pharmacology & Pharmacy
Guoping Shu, Yueli Tang, Mingyuan Yuan, Ning Wei, Fangyuan Zhang, Chunxian Yang, Xiaozhong Lan, Min Chen, Kexuan Tang, Lien Xiang, Zhihua Liao
Summary: The AabZIP1-AaMYC2 transcriptional module is revealed as a point of cross-talk between ABA and JA signaling in artemisinin biosynthesis, which may have general implications.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Plant Sciences
Yongpeng Li, Tiantian Chen, Hang Liu, Wei Qin, Xin Yan, Kuanyu Wu-Zhang, Bowen Peng, Yaojie Zhang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: A new promoter, tpACT, was discovered in this study, showing higher activity than the commonly used CaMV35S promoter in artemisinin-producing plants. Application of tpACT in genetic transformation of Artemisia annua can increase the production of artemisinin.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Qian Shen, Huayi Huang, Lihui Xie, Xiaolong Hao, Sadaf-Ilyas Kayani, Hang Liu, Wei Qin, Tiantian Chen, Qifang Pan, Pin Liu, Kexuan Tang
Summary: Plants have evolved complex systems to regulate the biosynthesis of specialized phytochemicals. Artemisinin, an important anti-malaria drug, is produced by the plant Artemisia annua. However, the amount of artemisinin in A. annua is low and cannot meet market demands. This study identified two transcription factors as novel regulators of artemisinin biosynthesis and proposed a model for how different transcription factors coordinate to regulate artemisinin production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Hang Liu, Ling Li, Xueqing Fu, Yongpeng Li, Tiantian Chen, Wei Qin, Xin Yan, Zhangkuanyu Wu, Lihui Xie, Sadaf-llyas Kayani, Danial Hassani, Xiaofen Sun, Kexuan Tang
Summary: In this study, a transcription factor named AaMYB108 was identified as a positive regulator of artemisinin biosynthesis in A. annua. AaMYB108 promotes artemisinin biosynthesis by interacting with a previously characterized positive regulator, AaGSW1. Additionally, AaMYB108 interacts with AaCOP1 and AaJAZ8, and its function is influenced by these proteins. Through experiments with transgenic plants, it was found that the promotion of artemisinin by light and JA depends on the presence of AaMYB108. Overall, this study reveals the molecular mechanism of JA regulating artemisinin biosynthesis depending on light and provides new insights into the integration of light and phytohormone signaling in plants.
Article
Plant Sciences
Sadaf-Ilyas Kayani, Yanan Ma, Xueqing Fu, Qian Shen, Yongpeng Li, Saeed-ur Rahman, Bowen Peng, Liu Huang, Kexuan Tang
Summary: This study reveals the interaction between AaYABBY5 and AaWRKY9, which indirectly regulates artemisinin production. AaYABBY5 promotes the activity of AaGSW1 and AaDBR2, while AaJAZ8 inhibits the activity of AaYABBY5. This study provides the molecular basis for the regulation of artemisinin biosynthesis through YABBY-WRKY interactions and highlights AaYABBY5 overexpression plants as a valuable genetic resource.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Tian-Tian Chen, Hang Liu, Yong-Peng Li, Xing-Hao Yao, Wei Qin, Xin Yan, Xiu-Yun Wang, Bo-Wen Peng, Yao-Jie Zhang, Jin Shao, Xin-Yi Hu, Xue-Qing Fu, Ling Li, Yu-Liang Wang, Ke-Xuan Tang
Summary: In this study, a MADS-box transcription factor AaSEP1, induced by jasmonate and light signaling, was identified as a positive regulator of glandular secretory trichome (GST) initiation in Artemisia annua. AaSEP1 enhanced the function of AaHD1 activation and interacted with AaMYB16 and AaJAZ8, contributing to GST initiation mediated by the jasmonate signaling pathway. AaSEP1 also interacted with AaCOP1, a major repressor of light signaling. This study provides important insights into the regulatory mechanism of GST initiation in A. annua.
Article
Biochemistry & Molecular Biology
Chen Wang, Tiantian Chen, Yongpeng Li, Hang Liu, Wei Qin, Zhangkuanyu Wu, Bowen Peng, Xiuyun Wang, Xin Yan, Xueqing Fu, Ling Li, Kexuan Tang
Summary: Exploring the genetic network of glandular trichomes and manipulating genes relevant to secondary metabolite biosynthesis are of great importance and value. Artemisinin, a key antimalarial drug ingredient, is synthesized and stored in glandular secretory trichomes (GSTs) in Artemisia annua. WIN/SHN proteins, a clade of AP2/ERF family, are known as regulators for cuticle biosynthesis. However, their function in glandular trichome development is less unknown.
Article
Plant Sciences
Danial Hassani, Ayat Taheri, Xueqing Fu, Wei Qin, Liu Hang, Yanan Ma, Kexuan Tang
Summary: The study focused on increasing the artemisinin content in Artemisia annua plants through genetic engineering. By overexpressing three artemisinin biosynthesis enzymes and two transcription factors, the content of artemisinin in the transgenic plants was significantly increased in both T0 and T1 generations.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Sadaf-Ilyas Kayani, Saeed-ur-Rahman, Qian Shen, Yi Cui, Wei Liu, Xinjuan Hu, Feifei Zhu, Shuhao Huo
Summary: Microalgae, especially Haematococcus pluvialis, are preferred for the production of astaxanthin due to their low toxicity risk compared to chemical synthesis. This critical review discusses the strategies employed to enhance astaxanthin biosynthesis, including cultivation conditions and transcriptional regulation by factors that are yet to be identified. The review also proposes future approaches such as cloning and overexpression of positive regulators, gene editing, and hormonal modulation of transcription factors for efficient astaxanthin production. Overall, this study provides significant insights into the regulation and improvement of astaxanthin biosynthesis in H. pluvialis.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Yinkai Yang, Chengyu Xiao, Yan Cai, Jingyi Wang, Qingyan Ruan, Miaomiao Sheng, Ling Li, Kexuan Tang, Guoyin Kai, Xiaolong Hao
Summary: This study revealed the synergistic effect of the phytohormone jasmonic acid (JA) and light on the biosynthesis of anti-malarial drug artemisinin in traditional Chinese medicinal plant Artemisia annua. A JA-responsive R2R3-MYB transcription factor, AaJRM1, was found to be highly expressed in trichomes and rapidly responded to JA treatment. Overexpression and suppression of AaJRM1 increased and decreased the accumulation of artemisinin, respectively. The study elucidated the molecular mechanism of JA-responsive AaJRM1 transcription factor regulating artemisinin biosynthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2023)