期刊
CHEMICO-BIOLOGICAL INTERACTIONS
卷 239, 期 -, 页码 111-117出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2015.06.039
关键词
Citronellol; Monoterpenes; Hyperalgesia; Spinal Cord; Fos; Pain
资金
- National Council of Technological and Scientific Development (CNPq/Brazil)
- Research Supporting Foundation of the State of Sergipe (FAPITEC-SE/Brazil)
We evaluated the anti-hyperalgesic effect of citronellol (CT) and investigated the spinal cord lamina I involvement in this effect. Male mice were pre-treated with CT (25, 50 and 100 mg/kg, i.p.), indomethacin (10 mg/kg, i.p.), dipyrone (60 mg/kg, i.p.) or vehicle (saline + Tween 80 0.2%). Thirty minutes after the treatment, 20 pi of carrageenan (CG; 300 mu g/paw), PGE(2) (100 ng/paw), dopamine (DA; 30 mu g/paw) or TNF-alpha, (100 pg/paw) were injected into the hind paw subplantar region and the mechanical threshold was evaluated with an electronic anesthesiometer. The CT effect on edema formation was evaluated after the right paw subplantar injection of CG (40 mu L; 1%) through the plethysmometer apparatus. To evaluate the CT action on the spinal cord, the animals were treated with CT (100 mg/kg; i.p.) or vehicle (Saline + Tween 80 0.2%; i.p.) and, after 30 min, 20 mu L of CG (300 mu g/paw; i.pl.) was injected. Ninety minutes after the treatment, the animals were perfused, the lumbar spinal cord collected, crioprotected, cut and submitted in an immunofluorescence protocol for Fos protein. CT administration produced a significantly reduction (p < 0.05) in the mechanical hyperalgesia induced by CG, TNF-alpha, PGE2 and DA when compared with control group. The treatment with CT also significantly (p < 0.05) decreased the paw edema. The immunofluorescence showed that the CT decrease significantly (p < 0.05) the spinal cord lamina I activation. Thus, our results provide that CT attenuates the hyperalgesia, at least in part, through the spinal cord lamina I inhibition. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据